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The regenerated plantlets were obtained from the cotyledons after shoot and root inducing cultivations. The PCR analysis showed that the hEGF gene has been integrated into the mini-tomato genome. Radioimmunoassay (RIA) showed that the mean yield of rhEGF in the fresh fruits of the transgenic mini-tomatoes amounted to 3.85 ± 0.86 ng /g fruit. Ingestion via gastric intubation of the transgenic mini-tomato juice significantly protected the mice from the alcohol-induced acute peptic ulceration. The ulcer index, expressed as a degree of the stomach lesion, decreased from 52.20±23.24 to 21.20±6.84,and the inhibition rate got to 59.39%., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=y8x8FzndVsX7R24rutJX0g==, pdfFileSize=217926, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242117860990976763, articleId=1242117859946595056, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=rhEGF转基因迷你番茄研究, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=为了使人表皮生长因子(hEGF)基因在番茄中表达,构建了hEGF基因的表达载体p2300-2A11-hEGF,通过电激法转化到农杆菌菌株C58中,采用叶盘法转化番茄品系cg2002-14。子叶经发芽及生根诱导培养后得到了再生植株。PCR分析表明,hEGF基因已整合到番茄的基因组中。放射免疫法(RIA)测得重组人表皮生长因子(rhEGF)在鲜重果实中平均含量为3.85±0.86ng/g,对酒引起的小鼠急性胃溃疡有明显的预防作用,溃疡指数由52.20±23.24降至21.20±6.84,抑制率达59.39%。, authors=智庆文^1，2，4,张凤英⁴,柴敏³,李仕贵2，孙曼霁¹, authorsList=智庆文;;张凤英;柴敏;李仕贵;孙曼霁, authorCompany=1. 军事医学科学院毒物药物研究所 北京 2 四川农业大学水稻研究所 成都3. 北京市农林科学院蔬菜研究所 北京 4. 中国人民解放军防化指挥工程学院 北京, correspAuthors=null, authorNote=null, correspAuthorsNote=智庆文, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=QieOsqCClXncNibaZKdDLQ==, pdfFileSize=217926, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=null, keywords=[Keyword(id=1242117860634460917, tenantId=1146029695717560320, 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