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Neighbor-Joining phylogenetic tree based on PAL sequence of bamboo and other 27 plants indicated that PAL gene sequence in Bamboo is relatively conservative，and bamboo had very close relationship with that of Zea may and Saccharum officinarum , and far relationship with that of Capsicum annuum et al., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=6GA2WRjblf9wF0iuYUHPOg==, pdfFileSize=1017530, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242117737955262724, articleId=1242117735036027124, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=4种竹子木质素合成酶PAL的基因克隆和序列分析, 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科技导报
|研究论文
2009
, 27
(0922) :
19
-25
4种竹子木质素合成酶PAL的基因克隆和序列分析
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李潞滨1 ,刘蕾2 ,张淑萍2，3 ,何聪芬2 ,胡桃1 ,庄彩云1
作者信息
1. 中国林业科学研究院林业研究所；国家林业局林木培育重点实验室，北京 1000912. 北京工商大学化学与环境工程学院；植物资源研究开发北京市重点实验室，北京 1000483. 首都师范大学生命科学学院，北京 100048
通讯作者:
何聪芬
Gene cloning and sequence analysis of phenylalanine ammonia-lyase (PAL) in bamboo lignin synthase
Affiliations
出版时间: 2009-11-28
文章导航
苯丙氨酸解氨酶（Phenylalanine Ammonia-Lyase，PAL；EC 4.3.1.5）是木质素生物合成过程的关键酶和限速酶。应用RACE（Rapid-Amplification of cDNA Ends）方法，获得了白哺鸡竹（Phyllostachys dulcis）、高节竹（Phyllostachys prominens）、龙鳞竹（Phyllostachys pubescens heterocycla）、丽水苦竹（Pleioblastus maculosoides Wen）等4种竹子PAL基因的全长序列，并进行了生物信息学分析。结果表明，PAL基因的开放读码框长度为2 136 bp，共编码712个氨基酸，具有2个外显子和1个内含子。其中高节竹、龙鳞竹、丽水苦竹PAL基因内含子长度为121 bp，白哺鸡竹PAL基因内含子长度为84 bp。推测其氨基酸序列，并分析PAL蛋白单体的三维结构，结果显示PAL蛋白均含有大量的α螺旋和β折叠结构。基于邻接法的进化树对31个物种的PAL基因的氨基酸序列分析表明，竹类植物PAL基因的保守性较高，与禾本科植物玉米、甘蔗的亲缘关系较近，与双子叶植物辣椒等的亲缘关系较远。
PAL (phenylalanine ammonia-lyase, EC 4.3.1.5) is one of the key enzymes in the process of lignin synthesis. In this paper, PAL gene sequence from four vaireties of bamboo: Phyllostachys dulcis, Phyllostachys prominens, Phyllostachys pubescens heterocycla, and Pleioblastus maculosoides Wenwere obtained by RACE (rapid-amplification of cDNA ends). The results show that each PAL gene has two exons and an intron and every ORF (Open Reading Frames) is 2136 bp length, encoding 712 amino acids. And then the bamboo PAL amino acid sequences and three-dimensional structures were speculated and analyzed, the results indicate that all of the PAL proteins contain a large number of α-helix and β plated sheet structure. Neighbor-Joining phylogenetic tree based on PAL sequence of bamboo and other 27 plants indicated that PAL gene sequence in Bamboo is relatively conservative，and bamboo had very close relationship with that of Zea may and Saccharum officinarum , and far relationship with that of Capsicum annuum et al.
bamboo
/
lignin
/
PAL
/
RACE
李潞滨;刘蕾;张淑萍;何聪芬;胡桃;庄彩云.
4种竹子木质素合成酶PAL的基因克隆和序列分析.
科技导报,
2009
, 27
(0922)
: 19
-25
.
.
Gene cloning and sequence analysis of phenylalanine ammonia-lyase (PAL) in bamboo lignin synthase[J].
Science & Technology Review ,
2009
, 27
(0922)
: 19
-25
.
2009年第27卷第0922期
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文章信息
接收时间：2009-10-10
首发时间：2009-11-28
出版时间：2009-11-28
收稿日期：2009-10-10
修回日期：2009-11-02
https://castjournals.cast.org.cn/joweb/kjdb/CN/1242117735036027124
引用本文
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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