Article(id=1242117529984897893, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242116865502282190, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1227542400000, receivedDateStr=2008-11-25, revisedDate=1231344000000, revisedDateStr=2009-01-08, acceptedDate=null, acceptedDateStr=null, onlineDate=1234454400000, onlineDateStr=2009-02-13, pubDate=1234454400000, pubDateStr=2009-02-13, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1234454400000, onlineIssueDateStr=2009-02-13, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774073496570, creator=sys-migrate, updateTime=1774073496570, updator=sys-migrate, issue=Issue{id=1242116865502282190, tenantId=1146029695717560320, journalId=1146031591421210625, year='2009', volume='27', issue='0903', pageStart='3', pageEnd='126', issueExtLink='null', onlineDate='null', pubDate='1234454400000', pubDateStr='2009-02-13', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774073338149, creator='sys-migrate', updateTime=1774073338149, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=0, endPage=0, ext={EN=ArticleExt(id=1242117531977187815, articleId=1242117529984897893, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=TANSHINONE EFFECT ON IRS-1, P-ERK EXPRESSION IN THE TROPHOBLASTIC CELLS UNDER INSULIN RESISTANCE, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=To investigate the expression of mitogen- activated protein kinase and insulin receptor substrate-1 in trophoblastic cell and the relationship between pertensive disorder complicating pregnancy.METHODS:(1)primary culture of human placental villus in vitro, trophoblastic cells were exposed to 2.5umol/L wortmannin for 48 hours to establish insulin-resistant trophoblastic cell. (2)Protein expression of insulin receptor substrate-1 and extracellular signal-regulated kinase was detected by western blotting RESULTS:(1)Under the microscope, the polygon cell grew monolayer slice and there were some syncytio- trophoblast by cell fusion. (2) Where there is insulin resistance, the level of insular signal transmission main protein IRS-1 rised 26.58% compared with normal. With treatment of tanshinone, the level of insular signal transmission main protein IRS-1 reduced 16.42% compared with model. With treatment of metformin, the level of insular signal transmission main protein IRS-1 reduced 6.66% compared with model.(3) Where there is insulin resistance, protein expression of extracellular signal-regulated kinase rised 56.93% compared with normal. With treatment of tanshinone, protein expression of extracellular signal-regulated kinase reduced 26.63% compared with model. With treatment of metformin, protein expression of extracellular signal-regulated kinase reduced 10.18% compared with model.CONCLUSIONS: When trophoblastic cell has insulin resistance, protein expression of IRS-1 and p-ERK rised. Use of tanshinone and metformin reduced protein expression of IRS-1 and p-ERK., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242117531826192870, articleId=1242117529984897893, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=丹参酮对滋养细胞胰岛素抵抗下IRS-1 、 p-ERK表达的影响, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=目的：研究不同药物干预组的绒毛膜滋养层细胞中胰岛素受体底物-1（IRS-1）和丝裂原活化蛋白激酶（MAPK）蛋白的表达及其与妊娠期高血压的关系。方法：(1)体外原代培养人绒毛膜滋养细胞，用沃漫青霉素作用于滋养细胞诱发胰岛素抵抗的细胞模型；(2)用蛋白印迹法检测胰岛素受体底物-1（IRS-1）和磷酸化细胞外信号调节激酶（p-ERK）在正常组、溶剂组、胰岛素抵抗组、丹参酮组和二甲双胍组中的变化。结果：（1）显微镜下可见细胞呈多边形，细胞平铺生长，有细胞融合后形成的合体滋养层细胞；（2）当细胞胰岛素抵抗时，IRS-1的GSR为0.6857，较正常对照组GSR（0.5417）升高了26.58%，加入药物改善胰岛素抵抗后丹参酮组的GSR（0.5890）降低了16.42%，二甲双胍组的GSR（0.6429）降低了6.66%；（3）在沃漫青霉素诱发胰岛素抵抗时，p-ERK的GSR为1.7，较正常对照组GSR（1.0833）升高了56.93%；加入药物改善胰岛素抵抗后丹参酮组的GSR（1.3425）较抵抗组降低了26.63%，二甲双胍组的GSR（1.5429）较抵抗组降低了10.18%。结论：1、丹参酮对IRS-1的胰岛素抵抗改善水平优于二甲双胍；2、丹参酮和二甲双胍对p-ERK均有降调作用；3、丹参酮通过对IRS-1、p-ERK的调节，改善了妊娠期高血压疾病的胰岛素抵抗状态。, authors=张跃辉¹,胡敏¹,吴效科¹,侯丽辉², authorsList=张跃辉;胡敏;吴效科;侯丽辉, authorCompany=1. 黑龙江中医药大学附属第一医院妇产科2. 黑龙江中医药大学附属第一医院, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, 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