Article(id=1242117110495772899, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242117110185398817, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=-2209017600000, receivedDateStr=1900-01-01, revisedDate=-2209017600000, revisedDateStr=1900-01-01, acceptedDate=null, acceptedDateStr=null, onlineDate=1235750400000, onlineDateStr=2009-02-28, pubDate=1235750400000, pubDateStr=2009-02-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1235750400000, onlineIssueDateStr=2009-02-28, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774073396560, creator=sys-migrate, updateTime=1774073396560, updator=sys-migrate, issue=Issue{id=1242117110185398817, tenantId=1146029695717560320, journalId=1146031591421210625, year='2009', volume='27', issue='0904', pageStart='3', pageEnd='126', issueExtLink='null', onlineDate='null', pubDate='1235750400000', pubDateStr='2009-02-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774073396484, creator='sys-migrate', updateTime=1774073396484, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=75, endPage=79, ext={EN=ArticleExt(id=1242117112903307826, articleId=1242117110495772899, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Tanshinone Effect on theExpression of IRS-1 and p-ERK in Trophoblastic Cells Under Insulin Resistance, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract= This paper investigates the expression of mitogen-
activated protein kinase and insulin receptor substrate-1 in
trophoblastic cells and the relationship with hypertensive disorder complicating pregnancy. With primary culture of human placental villus in vitro, trophoblastic cells were exposed to 2.5 ?滋mol/L wortmannin for 48 hours to establish insulin-resistant trophoblastic cell. Protein expression of insulin receptor substrate-1 and extracellular signal-regulated kinase was obtained by western
blotting. Under the microscope, the polygon cell took the shape of monolayer slice and there were some syncytio-trophoblasts by cell fusion; with insulin resistance, the level of insular signal transmission of the main protein IRS-1 was risen 26.58% above the normal level. With treatment of tanshinone, it was reduced 16.42% as compared with the model. With treatment of metformin, the level of insular signal transmission of the main protein IRS-1 reduced 6.66% as compared with the model. With insulin resistance, protein expression of extracellular signal-regulated kinase was risen 56.93% above the normal level. With treatment of tanshinone, protein expression of extracellular signal-regulated kinase reduced 26.63% as compared with the model. With treatment of metformin, protein expression of extracellular signal-regulated kinase reduced 10.18% as compared with the model. It is concluded that with the trophoblastic cell in insulin resistance, the protein expression of IRS-1 and p-ERK will rise. Use of tanshinone2 and metformin will reduce the protein expression of IRS-1 and p-ERK., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242117112446128686, articleId=1242117110495772899, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=丹参酮对滋养细胞胰岛素抵抗下IRS-1和p-ERK表达的影响, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract= 为了不同药物干预组的绒毛膜滋养层细胞中胰岛素受体底物-1（IRS-1）和丝裂原活化蛋白激酶（MAPK）蛋白的表达及其与妊娠期高血压的关系，采用以下方法：① 体外原代培养人绒毛膜滋养细胞，用沃漫青霉素作用于滋养细胞诱发胰岛素抵抗的细胞模型；② 用蛋白印迹法检测IRS-1和磷酸化细胞外信号调节激酶（p-ERK）在正常组、溶剂组、胰岛素抵抗组、丹参酮组和二甲双胍组中的变化。结果显示：① 显微镜下可见细胞呈多边形，细胞平铺生长，有细胞融合后形成的合体滋养层细胞；② 当细胞胰岛素抵抗时，IRS-1的GSR为0.685 7，较正常对照组GSR（0.541 7）升高了26.58%，加入药物改善胰岛素抵抗后，丹参酮组的GSR（0.589 0）降低了16.42%，二甲双胍组的GSR（0.642 9）降低了6.66%；③ 在沃漫青霉素诱发胰岛素抵抗时，p-ERK的GSR为1.7，较正常对照组GSR（1.083 3）升高了56.93%；加入药物改善胰岛素抵抗后丹参酮组的GSR（1.342 5）较抵抗组降低了26.63%，二甲双胍组的GSR（1.542 9）较抵抗组降低了10.18%。由此得出结论：① 丹参酮2对IRS-1的胰岛素抵抗改善水平优于二甲双胍；② 丹参酮2和二甲双胍对p-ERK均有降调作用；③ 丹参酮2通过对IRS-1、p-ERK的调节，改善了妊娠期高血压疾病的胰岛素抵抗状态。, authors=行俊秀，张跃辉，胡 敏，胥风华，吴效科，侯丽辉, authorsList=行俊秀;张跃辉;胡 敏;胥风华;吴效科;侯丽辉, authorCompany=黑龙江中医药大学附属第一医院妇产科, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, 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