Article(id=1242117012353254295, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242116994296778923, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1239120000000, receivedDateStr=2009-04-08, revisedDate=1248019200000, revisedDateStr=2009-07-20, acceptedDate=null, acceptedDateStr=null, onlineDate=1250092800000, onlineDateStr=2009-08-13, pubDate=1250092800000, pubDateStr=2009-08-13, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1250092800000, onlineIssueDateStr=2009-08-13, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774073373160, creator=sys-migrate, updateTime=1774073373160, updator=sys-migrate, issue=Issue{id=1242116994296778923, tenantId=1146029695717560320, journalId=1146031591421210625, year='2009', volume='27', issue='0915', pageStart='3', pageEnd='140', issueExtLink='null', onlineDate='null', pubDate='1250092800000', pubDateStr='2009-08-13', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774073368854, creator='sys-migrate', updateTime=1774073368854, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=44, endPage=47, ext={EN=ArticleExt(id=1242117015662560180, articleId=1242117012353254295, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Influence of Bacterial Endotoxin on Survival Rate and Birth Rate of Genetic Immunised Mice, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=As a third generation vaccine, the nucleic acid vaccine is easy to operate, and of low cost. However, there are some intrinsic risks involved, such as bacterial endotoxin, which should be studied and avoided. Bacterial endotoxin is contained in the outer layer surrounding cell wall of Gram negative bacteria. After being released in vivo, it can cause many pathological changes, such as fever and shock. Some previous in vitro studies show that endotoxin had bad effects on reproductive system of mice. This paper presents experimental studies on the effect of endotoxin on the survival rate and birth rate of immunised mice in vivo. In the experiment, two types of pCR3.1-Sry were extracted by two different methods and used to immunise mice. One method uses the general method, and the other uses a reagent kit. Their difference lies in the fact whether the endotoxin is removed or not. And, their purity and concentration were analyzed and controlled by agarose gel electrophoresis and spectrophotometry. At the same time, sterile ddH2O was injected into mice as control. Then, the immunising and mating tests of mice were carried out. Molecular biological results show that two types of nucleic acid vaccine were at proper concentration and high purity too. The immunity and statistical results show that compared with the group whose endotoxin was removed, the survival rate and the birth rate of the group with endotoxin were severally decreased from 93% to 84% and from 87.5% to 35.7%, respectively. At the same time, data for endotoxin-removed group is close to the control group. All the results indicate that endotoxin interfused in the nucleic acid vaccine has adverse effect on immunised mice, especially, on the reproductive system., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=3FLdhxpt01pvc0Tu89nXMA==, pdfFileSize=396686, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242117014735622380, articleId=1242117012353254295, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=细菌内毒素对基因免疫小鼠存活率和产仔率的影响, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=作为第三代疫苗，核酸疫苗具有易操作、低成本等优点，但是其内在危险性还有待进一步研究和排除，在其制备过程中可能掺入的细菌内毒素就是影响因素之一。细菌内毒素是革兰氏阴性菌细胞壁外膜结构中的脂多糖，体内释放后会引起发热、休克等多种病理变化。国外已有体外实验证明内毒素会对小鼠的生殖系统产生影响，本研究旨在通过在体实验探讨内毒素对免疫小鼠的存活率和产仔率的影响。采用常规方法（碱裂解法）、试剂盒2种方法，提取2种pCR3.1-Sry质粒（2种质粒的区别在于是否去除内毒素，其浓度和纯度均经过琼脂糖凝胶电泳和紫外分光光度法分析和控制），作为核酸疫苗对小鼠进行免疫；全程统计免疫小鼠的存活率和产仔率，同时注射灭菌ddH2O作为对照。分子生物学实验结果显示，所制备疫苗具有适宜的浓度和较高的纯度；免疫实验和数字统计结果显示，未去除内毒素实验组小鼠较去除内毒素组的存活率由93%下降到84%、产仔率由87.5%下降到35.7%，而去除内毒素组却与对照组没有明显差异。总之，核酸疫苗制备过程中掺入的细菌内毒素会对免疫小鼠造成较大不良影响，特别是生殖系统，体现在产仔率大幅降低。, authors=张艳萍, authorsList=张艳萍, authorCompany=青岛农业大学生命科学学院, correspAuthors=null, authorNote=null, correspAuthorsNote=朱宝长, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=cOMCuxDdHnYebcGUZwKa+w==, pdfFileSize=396686, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, 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