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物理化学诱变选育冠菌素高产菌株
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科技导报 |研究论文 2009 , 27 (0912) : 34 -37
物理化学诱变选育冠菌素高产菌株
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吴慧玲,李召虎
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    中国农业大学农学与生物技术学院
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吴慧玲
Screening of High Coronatine Producing Mutants by Mutagenized with UV and NTG
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    出版时间: 2009-06-28
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    冠菌素是一种能够引起大豆叶片黄萎病的致病因子。它作为茉莉酸类物质的结构类似物在植物抵抗各种环境胁迫中起着重要作用。但是目前冠菌素产量比较低,这就限制了冠菌素生理作用的广泛研究。本试验通过紫外、亚硝基胍结合诱变选育冠菌素高产突变体,得到的突变体冠菌素产量是出发菌株的1.8倍,命名为M-18。该菌株在18℃下发酵生产冠菌素,产量大幅度提高,发酵周期明显缩短。
    冠菌素  /  丁香假单胞菌大豆致病变种  /  紫外-亚硝基胍
    Coronatine(COR) is a phytotoxin that can induce bacterial blight in soybean (Glycinea max L.). It is a structural and functional analogue of jasmonic acid, produced by many plant species in response to stress. COR might be employed in agriculture to elicit plant resistance against various aggressors. But the low yield of COR has limited its applications in research. In our study, COR biosynthesis is enhanced by combination of ultraviolet (UV) radiation and N-methyl-N′-nitro-N-nitrosoguanidine (NTG) mutagenesis in Pseudomonas syringae pv. glycinea PG4180. Wild-type P. syringae pv. glycinea PG4180 is exposed to UV and NTG, to create a mutant M-18 with a high yield of COR. The yield of COR of strain M-18 is increased about 1.8-fold and reaches the maximum COR production in less than one-half of the time that the wild-type P. syringae pv. glycinea PG4180 takes. The pH of the culture medium is decreased much earlier with the mutant M-18 than the wild-type, which confirms that the fermentation period of the mutant is shorter than that of the wild strain.
    coronatine  /  P.syringae pv.Glycinea  /  UV-NTG
    吴慧玲;李召虎. 物理化学诱变选育冠菌素高产菌株. 科技导报, 2009 , 27 (0912) : 34 -37 .
    . Screening of High Coronatine Producing Mutants by Mutagenized with UV and NTG[J]. Science & Technology Review, 2009 , 27 (0912) : 34 -37 .

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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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