Article(id=1242116072778830342, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242116066525118632, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1241971200000, receivedDateStr=2009-05-11, revisedDate=-2209017600000, revisedDateStr=1900-01-01, acceptedDate=null, acceptedDateStr=null, onlineDate=1246118400000, onlineDateStr=2009-06-28, pubDate=1246118400000, pubDateStr=2009-06-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1246118400000, onlineIssueDateStr=2009-06-28, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774073149146, creator=sys-migrate, updateTime=1774073149146, updator=sys-migrate, issue=Issue{id=1242116066525118632, tenantId=1146029695717560320, journalId=1146031591421210625, year='2009', volume='27', issue='0912', pageStart='3', pageEnd='126', issueExtLink='null', onlineDate='null', pubDate='1246118400000', pubDateStr='2009-06-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774073147655, creator='sys-migrate', updateTime=1774073147655, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=19, endPage=22, ext={EN=ArticleExt(id=1242116081456845355, articleId=1242116072778830342, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=The Cloning, Expression and Purification of F3 Recombinant Toxin, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=F3 is inserted into PQE80L carrier, after being amplified by PCR, with the plasmid pEKH-F3 as the template, to obtain PQE80L－F3 recombinated plasmid. With extracted pseudomonas aeruginosa strain chromosome DNA as the template, PE40, the pseudomonas aeruginosa exotoxin A catalysis area, is amplified by PCR. Then PQE80-F3 is recombinated with PE40, to obtain PQE80L-F3-PE40 carrier. The DH5a and BL21 are obtained by transformation. The results show that F3－PE40 fusion protein is expressed and the quantity of expressed fusion protein accounts for about 20% of the total bacterial proteins. Through extraction of plasmid, PCR amplification, and F3-PE40 construction, E. coli expression vector is obtained by transformation to successfully express the fusion protein F3-PE40, which lays a foundation for large-scale expression and purification of F3-PE40 for further functionality., authors=null, authorsList=null, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=PMygzWeHICU2eDDeQh46rA==, pdfFileSize=446522, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242116080542487078, articleId=1242116072778830342, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=F3重组毒素的克隆表达及纯化, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=以pEKH-F3 质粒为模板，PCR扩增F3片段，将F3插入克隆质粒PQE80L，转化大肠杆菌，筛选，获得含有F3的PQE80L重组载体的克隆。提取绿脓杆菌菌株染色体DNA为模板，PCR扩增绿脓杆菌外毒素A催化区，PE40。然后将PQE80-F3和PE40重组，得到表达PQE80L-F3-PE40载体。转化至大肠杆菌DH5a,BL21，表达融合蛋白F3-PE40。结果显示，大肠杆菌表达了融合蛋白F3-PE40，表达的融合蛋白量大概占菌体总体蛋白量的20%。通过质粒提取、PCR扩增构建F3-PE40表达载体转化大肠杆菌，成功地表达了融合蛋白F3-PE40，为大规模表达、纯化F3-PE40的进一步功能奠定了基础。, authors=朱海兵,曾昭淳,黄轶, authorsList=朱海兵;曾昭淳;黄轶, authorCompany=重庆医科大学生物化学及分子生物学教研室, correspAuthors=null, authorNote=null, correspAuthorsNote=曾昭淳, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=eqUDU19PPziG9yi9YdAWMQ==, pdfFileSize=446522, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null)}, authors=null, 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