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细胞膜蛋白是药物作用的关键靶点,而核酸适体是一类能够高特异性、高亲和力结合靶标分子的寡核苷酸。然而,传统的核酸适体筛选方法效率低、过程繁杂,且难以在生理相关环境下系统发现全新的疾病标志物。

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    细胞膜蛋白是药物作用的关键靶点,而核酸适体是一类能够高特异性、高亲和力结合靶标分子的寡核苷酸。然而,传统的核酸适体筛选方法效率低、过程繁杂,且难以在生理相关环境下系统发现全新的疾病标志物。

    . 癌细胞表面布下雷达网可实现精准医疗. 科技导报, 2026 , 44 (1) : 2 -2 .
    . Science & Technology Review, 2026 , 44 (1) : 2 -2 .
    细胞膜蛋白是药物作用的关键靶点,而核酸适体是一类能够高特异性、高亲和力结合靶标分子的寡核苷酸。然而,传统的核酸适体筛选方法效率低、过程繁杂,且难以在生理相关环境下系统发现全新的疾病标志物。
    中国科学院院士、中国科学院杭州医学所所长谭蔚泓与中国科学院杭州医学所研究员吴芩团队成功构建了全球首个在单细胞分辨率下,同步实现细胞膜表面标志物发现与靶向核酸适体探针获取的一体化平台——SPARK-seq,重新定义了核酸适体的筛选与分析范式。2026年1月1日,相关研究成果发表于《Science》。
    SPARK-seq平台的诞生解决了这一挑战。相较于传统方法,SPARK-seq平台筛选效率提升达百倍以上,并能以前所未有的精度锁定潜在的癌症标志物与治疗靶点。“我们研发SPARK-seq的初衷,是为了攻克像三阴性乳腺癌这类缺乏明确治疗靶点的临床难题。”吴芩表示,该平台在单细胞层面部署的“分子雷达”,能够大规模、并行地精准识别细胞表面与疾病相关的靶标,并同步获取能特异性结合它的核酸适体探针。
    SPARK-seq工作原理主要分为3个关键步骤:第1步,利用CRISPR基因编辑技术对大量癌细胞进行“微创手术”,精准剪掉特定的膜蛋白;第2步,引入包含海量候选分子的核酸适体库;第3步,借助单细胞测序技术,为每个细胞拍摄“高清全景照”,在同一细胞里同步读取“谁被剪掉了”“细胞变了吗”“谁结合上去了”的关键信息。
    SPARK-seq作为自主可控的原创性平台,为“无药可靶”疾病的靶点研究与治疗开发提供了全新范式,使得科研与临床工作者能够基于中国独特的临床资源,系统性地发现具有自主知识产权的新靶点与新工具。
    (综合:《Science》、科学网、新华网)

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    2种不同金属材料的力学参数

    Family
    属数
    Number of
    genus
    种数
    Number of
    species
    占总种数比例
    Percentage of
    total species (%)

    Genus
    种数
    Number of
    species
    占总种数比例
    Percentage of total
    species (%)
    鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
    小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
    多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
    红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
    小菇属 Mycena 11 5.26
    光柄菇属 Pluteus 5 2.39
    红菇属 Russula 17 8.13
    栓菌属 Trametes 5 2.39
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