Article(id=1242134487262376917, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242134390566887892, articleNumber=null, orderNo=42, doi=10.3981/j.issn.1000-7857.2016.2.036, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1428854400000, receivedDateStr=2015-04-13, revisedDate=1434384000000, revisedDateStr=2015-06-16, acceptedDate=null, acceptedDateStr=null, onlineDate=1454555982870, onlineDateStr=2016-02-04, pubDate=1453910400000, pubDateStr=2016-01-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1454555982870, onlineIssueDateStr=2016-02-04, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774077539495, creator=sys-migrate, updateTime=1774077539495, updator=sys-migrate, issue=Issue{id=1242134390566887892, tenantId=1146029695717560320, journalId=1146031591421210625, year='2016', volume='34', issue='2', pageStart='1', pageEnd='332', issueExtLink='null', onlineDate='null', pubDate='1453910400000', pubDateStr='2016-01-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774077516449, creator='sys-migrate', updateTime=1774077516449, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=215, endPage=220, ext={EN=ArticleExt(id=1242134493205701606, articleId=1242134487262376917, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Molecular cloning and sequence analysis of sodium-bile acid cotransporter from Echinococcus granulosus in Xinjiang, China, columnId=1242116810380743325, journalTitle=Science & Technology Review, columnName=Reviews, runingTitle=null, highlight=null, articleAbstract=Sodium-bile acid cotransporter (EgSBACT) functions in the sodium-bile acid transportation in Echinococcus granulosus (Eg) Protoscolex and the sequence is unknown. The specific primers for EgSBACT mRNA are designed and the EgSBACT gene is amplified by RT-PCR from Eg protoscolex total RNA. Then the EgSBACT gene fragment is cloned into pET30a vector which is a prokaryotic expression vector for sequencing and analyzing by bioinformatics method. The PCR products of EgSBACT is about 650 bp whose length is 654 bp and encodes a protein containing 217 amino acids and the pI of this protein is predicted as 9.28. Homology analysis shows that there is 43% of similarity between EgSBACT and the trematode SBACT (GenBank: GAA57409.1). Phylogenetic analysis shows that EgSBACT is clustered with Fluke, and has a lower homology to other species. EgSBACT gene is cloned from protoscolex of Echinococcus granulosus in Xinjiang, which lays a foundation for further study of EgSBACT function in the development and differentiation of PSCs into adult worms. This research provides a way to express the EgSBACT protein in vitro and taking advantage of this protein further research can reveal the exact functions of EgSBACT in the sodium-bile acid transportation in Echinococcus granulosus., authors=YANG Mei^1,2, LIANG Xiaodi², LI Jun³, LI Liang³, ZHANG Fuchun¹, ZHANG Wenbao³, authorsList=YANG Mei, LIANG Xiaodi, LI Jun, LI Liang, ZHANG Fuchun, ZHANG Wenbao, authorCompany=1. Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, China;
2. Basic Medical College, Xinjiang Medical University, Urumqi 830011, China;
3. State Key Laboratory Incubation Base of Xinjiang Major Diseases Research, the First Affiliated Hospital of Xinjiang Medical University, Urumqi 830011, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=WBQW+Oif1M2qD2zTCOwx6A==, pdfFileSize=5247468, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242134490479403972, articleId=1242134487262376917, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=细粒棘球绦虫新疆株胆汁酸钠协同转运蛋白基因的克隆及序列分析, columnId=1242116809164390686, journalTitle=科技导报, columnName=综述文章, runingTitle=null, highlight=null, articleAbstract=从新疆株细粒棘球绦虫(Echinococcus granulosus,Eg)原头蚴中克隆胆汁酸钠协同转运蛋白(sodium-bile acid co-transporter,EgSBACT)基因,进行序列测定和生物信息学分析。首先设计EgSBACT 基因特异性引物,用RT 及PCR 方法从新疆株细粒棘球绦虫原头蚴提取总RNA,将其反转录成cDNA 后扩增EgSBACT 基因并将其克隆至原核表达载体pET30a 中,测序鉴定序列并进行生物信息学分析。结果显示,RT-PCR 扩增出一长度约650 bp 的基因,测序显示其长度为654 bp,序列与Gen-bank 公布的EUB59978.1 完全一致,其编码217 个氨基酸,预测其等电点为9.28。同源性分析发现,EgSBACT 蛋白序列与中华枝睾吸虫SBACT(GenBank:GAA57409.1)同源性最高,达到43%。进化树分析表明,细粒棘球绦虫的SBACT 蛋白与吸虫纲的SBACT 相聚集,在进化树上处于一枝,而与其他物种亲缘性较远。由此表明,本研究成功克隆出细粒棘球绦虫EgSBACT 基因。, authors=杨梅^1,2, 梁小弟², 李军³, 李亮³, 张富春¹, 张文宝³, authorsList=杨梅, 梁小弟, 李军, 李亮, 张富春, 张文宝, authorCompany=1. 新疆大学生命科学与技术学院, 新疆生物资源基因工程重点实验室, 乌鲁木齐 830046;
2. 新疆医科大学基础医学院, 乌鲁木齐 830011;
3. 新疆医科大学第一附属医院, 新疆重大疾病医学重点实验室-省部共建国家重点实验室培育基地, 乌鲁木齐 830011, correspAuthors=null, authorNote=杨梅,博士研究生,研究方向为新疆重大疾病的分子生物学机制,电子信箱:768144163@qq.com, correspAuthorsNote=张文宝,研究员,研究方向为包虫病的防控,电子信箱:wenbaozhang2013@163.com;张富春(共同通信作者),教授,研究方向为包虫病的防控,电子信箱:zfcxju@xju.edu.cn, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=YuwyI+O+O3VVW0u5HY4jeg==, pdfFileSize=5247468, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=国家自然科学基金项目(31260272);新疆医科大学科研创新基金项目(XYDCX201423))}, authors=[Author(id=1280066514652999682, tenantId=1146029695717560320, journalId=null, articleId=1242134487262376917, orderNo=null, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=null, email=null, emailSecond=null, emailThird=null, correspondingAuthor=null, authorType=null, ext={EN=AuthorExt(id=null, tenantId=null, journalId=1146031591421210625, articleId=1242134487262376917, authorId=1280066514652999682, language=EN, stringName=YANG Mei, LIANG Xiaodi, LI Jun, LI Liang, ZHANG Fuchun, ZHANG Wenbao, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=null, tenantId=null, journalId=1146031591421210625, articleId=1242134487262376917, authorId=1280066514652999682, language=CN, stringName=杨梅, 梁小弟, 李军, 李亮, 张富春, 张文宝, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=null, address=null, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=null)], 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