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科技导报
| 专题论文 2014, 32(12): 26-30
粉红粘帚霉ACM941固体发酵条件的优化
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作者信息
通讯作者:
刘伟成，研究员，研究方向为植物真菌病害及其生物防治，电子信箱：liuwich@163.com
Optimization of Solid Fermentation of Gliocladium roseum ACM941
Affiliations
出版时间: 2014-04-28
doi: 10.3981/j.issn.1000-7857.2014.12.003
文章导航
为提高粉红粘帚霉ACM941 发酵过程中的分生孢子产量，本研究对其固体发酵条件进行了优化。以产孢量为测定指标，通过单因子和正交试验，确定ACM941 固体发酵基础培养基为麦麸和棉籽壳混合物，其比例为4：6；在基础培养基中添加2%玉米粉、2%燕麦粉、3%牛肉膏、2%蛋白胨可获得更大产孢量；对发酵温度、接种量等发酵条件进行了优化，确定发酵培养基初始含水量为30%；种龄48 h；接种量6%；发酵过程中24 h 全光照；菌丝生长温度25℃，产孢温度20℃。在最佳发酵条件下，ACM91产孢量达1.17×109 cfu/g 培养物，比初始发酵水平提高了12.4 倍，为生防制剂的开发奠定了基础。
粉红粘帚霉
/
孢子浓度
/
固体发酵培养基
/
发酵条件
The solid fermentation of Gliocladium roseum ACM941 has not been well studied so far,this paper proposes to improve the fermentation titer of ACM941 through the single factor and orthogonal design. The optimum fermentation conditions are obtained as follows: The bran and cottonseed shell ratio is 4:6, the corn flour 2%, the oat flour 2%, the beef extract 3%, and the peptone 2%. The optimum mycelial growth and sporulation temperatures are 25℃ and 20℃. The initial water content is 30% and the inoculation volume is 6%. Under these conditions, the maximum conidial yield comes to 1.17×109 cfu/g, 12.4 times higher than the initial fermentation level. This provides a support for the development of the biocontrol agent.
Clonostachys rosea
/
spore concentration
/
solid-state fermentation
/
fermentation conditions
张涛涛, 刘伟成, 吴慧玲, 董丹, 刘霆.
粉红粘帚霉ACM941固体发酵条件的优化.
科技导报,
2014
, 32
(12)
: 26
-30
.
DOI: 10.3981/j.issn.1000-7857.2014.12.003
ZHANG Taotao, LIU Weicheng, WU Huiling, DONG Dan, LIU Ting.
Optimization of Solid Fermentation of Gliocladium roseum ACM941[J].
Science & Technology Review ,
2014
, 32
(12)
: 26
-30
.
DOI: 10.3981/j.issn.1000-7857.2014.12.003
2014年第32卷第12期
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文章信息
doi: 10.3981/j.issn.1000-7857.2014.12.003
接收时间：2014-01-27
首发时间：2014-05-13
出版时间：2014-04-28
收稿日期：2014-01-27
修回日期：2014-03-26
通讯作者:
刘伟成，研究员，研究方向为植物真菌病害及其生物防治，电子信箱：liuwich@163.com
https://castjournals.cast.org.cn/joweb/kjdb/CN/10.3981/j.issn.1000-7857.2014.12.003
引用本文
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2种不同金属材料的力学参数
科 属数 种数 占总种数比例 属 种数 占总种数比例 鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78 小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39 多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39 红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87 小菇属 Mycena 11 5.26 光柄菇属 Pluteus 5 2.39 红菇属 Russula 17 8.13 栓菌属 Trametes 5 2.39
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