Article(id=1242131306939756638, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242131258810118155, articleNumber=null, orderNo=null, doi=10.3981/j.issn.1000-7857.2013.35.011, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1372348800000, receivedDateStr=2013-06-28, revisedDate=1383580800000, revisedDateStr=2013-11-05, acceptedDate=null, acceptedDateStr=null, onlineDate=1387296000000, onlineDateStr=2013-12-18, pubDate=1387296000000, pubDateStr=2013-12-18, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1387296000000, onlineIssueDateStr=2013-12-18, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774076781246, creator=sys-migrate, updateTime=1774076781246, updator=sys-migrate, issue=Issue{id=1242131258810118155, tenantId=1146029695717560320, journalId=1146031591421210625, year='2013', volume='31', issue='35', pageStart='3', pageEnd='96', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774076769767, creator=sys-migrate, updateTime=1774076769767, updator=sys-migrate, preIssue=null, nextIssue=null, ext=null, issueFiles=null}, startPage=60, endPage=63, ext={EN=ArticleExt(id=1242131315353526989, articleId=1242131306939756638, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Construction of Lentivirus-mediated HPV16-E7 Gene RNAi Cell Model, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=The HPV16-E7 ggene RNAi cell model was constructed. A recombinant lentiviral siRNA expression vector was constructed using a HPV16-E7 oncogene specific siRNA fragment, and an RNAi cell model stably expressing the HPV16-E7-siRNA was established by transfection of 293FT virus-packiging cells with the viral vector followed by infection of HPV16-positive SiHa carcinoma cells with recombinant virus, antibiotica selection and molecular biological characterization. RNAi cell model was successfully established, stably transcribing the HPV16-E7-siRNA fragment, and the inhitory effect on target gene (E7) was also characterized by western blotting and RT-PCR. The RNAi cell model expressing the HPV16-E7 gene-specific siRNA fragment using lentiviral vector was stable, effective and feasible, which lays the foundation for the role study and carcinogenic mechanism of E7 protein., correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=q4hIOtPeYFeVQjbnojLgZQ==, pdfFileSize=1010925, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. The First Afiliated Hospital, Xinjiang Medical University, Urumqi 830011, China;2. College of Basic Medicine, Xinjiang Medical University, Urumqi 830011, China;3. Xinjiang Key Laboratory of Molecular Biology and Endemic Diseases, Xinjiang Medical University, Urumqi 830011, China, fund=null, authors=ABUDUKADEER Abida¹, HASIMU Axiangu², AINIWAER Saimire², ABULIZI Abudula³, SHENG Lei³, authorsList=ABUDUKADEER Abida;HASIMU Axiangu;AINIWAER Saimire;ABULIZI Abudula;SHENG Lei), CN=ArticleExt(id=1242131311062753967, articleId=1242131306939756638, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=慢病毒介导的HPV16-E7基因RNAi细胞模型的建立, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=建立HPV16-E7基因的RNAi细胞模型，可为进一步研究HPV16-E7蛋白作用及致癌机制提供物质前提。选择HPV16-E7基因特异的siRNA片段，构建慢病毒siRNA重组表达载体，经293FT病毒包装细胞转染产生重组病毒，并以此感染HPV16阳性SiHa宫颈癌细胞、抗菌素筛选和分子生物学鉴定，建立了稳定表达HPV16-E7-siRNA的RNAi细胞模型。该细胞模型，对目标基因（E7）表达的抑制效率，也以蛋白免疫印迹和RT-PCR确证。由此得出，利用慢病毒载体和HPV16-E7基因特异的siRNA片段，可以建立一种稳定、有效和可行的RNAi细胞模型，为进一步研究E7蛋白的作用及致癌机制奠定了重要的物质基础。, correspAuthors=null, authorNote=null, correspAuthorsNote=盛磊，实验师，研究方向为宫颈癌的发病机制与早期预警，电子信箱：shenglei950505@163.com, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=tosfGdwcLbBbyVSzTqdM8Q==, pdfFileSize=1010925, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=1. 新疆医科大学第一附属医院, 乌鲁木齐 830011;2. 新疆医科大学基础医学院, 乌鲁木齐 830011;3. 新疆医科大学新疆地方病分子生物学重点实验室, 乌鲁木齐 830011, fund=null, authors=阿比达·阿不都卡德尔¹, 阿仙姑·哈斯木², 赛米热·艾尼瓦尔², 阿布力孜·阿布杜拉³, 盛磊³, authorsList=阿比达·阿不都卡德尔;阿仙姑·哈斯木;赛米热·艾尼瓦尔;阿布力孜·阿布杜拉;盛磊)}, authors=null, keywords=[Keyword(id=1242131309884154533, tenantId=1146029695717560320, journalId=1146031591421210625, articleId=1242131306939756638, language=CN, orderNo=1, keyword=RNAi), Keyword(id=1242131310064509606, tenantId=1146029695717560320, journalId=1146031591421210625, 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