Article(id=1242128914085122150, tenantId=1146029695717560320, journalId=1146031591421210625, issueId=1242128894485139484, articleNumber=null, orderNo=null, doi=10.3981/j.issn.1000-7857.2012.15.009, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1330272000000, receivedDateStr=2012-02-27, revisedDate=1335369600000, revisedDateStr=2012-04-26, acceptedDate=null, acceptedDateStr=null, onlineDate=1338134400000, onlineDateStr=2012-05-28, pubDate=1338134400000, pubDateStr=2012-05-28, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1338134400000, onlineIssueDateStr=2012-05-28, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774076210753, creator=sys-migrate, updateTime=1774076210753, updator=sys-migrate, issue=Issue{id=1242128894485139484, tenantId=1146029695717560320, journalId=1146031591421210625, year='2012', volume='30', issue='15', pageStart='3', pageEnd='95', issueExtLink='null', onlineDate='null', pubDate='1338134400000', pubDateStr='2012-05-28', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=3, issueType=-1, specialIssue=null, createTime=1774076206082, creator='sys-migrate', updateTime=1774076206082, updator='sys-migrate', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=57, endPage=60, ext={EN=ArticleExt(id=1242128917235044467, articleId=1242128914085122150, tenantId=1146029695717560320, journalId=1146031591421210625, language=EN, title=Effects of Xipayi Mouth Rinse on the PGE₂ Production of Human Gingival Fibroblast (HGF) Induced by IL-1β, columnId=1242116080374710456, journalTitle=Science & Technology Review, columnName=Articles, runingTitle=null, highlight=null, articleAbstract=To observe the effects of Xipayi mouth rinse on the prostaglandin E₂ (PGE₂) production of Human Gingival Fibroblast (HGF) by stimulation of interleukin-1β (IL-1β), the HGF cells cultured by healthy human gingival tissue are used. With 1ng/mL IL-1β as a stimulating factor, HGF cells with 5×10⁴/mL cell density were inoculated into a culture plate with 24 holes, each containing 200μL cell suspension. 24h after incubation and adhering to the wall, the original culture liquid was abandoned and the mouth was washed 2 times after adding the drugs, which were grouped into control group (with 20m/L fetal bovine serum medium DMEM), IL-1β group (beta concentration is 1ng/mL), IL-1β+Xipayi mouth rinse group (end concentrations of were 50, 100, 200g/mL, respectively ), IL-1β+indomethacin (100ng/mL of endcentration) group. The enzyme immunoassay method is used to check the PGE₂ and observe the effects of different concentrations of Xipayi mouth rinse on PGE₂ levels in the HGF supernatant. Results show that Xipayi mouth rinse at different concentrations suppresses the PGE₂ production of HGF stimulated with 1ng/mL IL-1β significantly in a dose dependent manner. It is concluded that the human gingival fibroblast has the ability of synthesizing and secreting PGE₂, and IL-1β is a potent stimulation for HGF cell to generate PGE₂; and Xipayi mouth rinse can significantly inhibit the PGE₂ production of HGF by stimulation of IL-1β., authors=YUSUF Abibai¹, AIZEZI Renaguli², UPUR Halmurat², authorsList=YUSUF Abibai;AIZEZI Renaguli;UPUR Halmurat, authorCompany=1. Department of Endocrinology, The First Affiliated Hospital of Xinjiang Medical University, Urumqi 830054, China;2. Department of Uygur Medicine, Xinjiang Medical University, Urumqi 830054, China, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=SRtkgUvfBU8hTdsASSBXkQ==, pdfFileSize=1522631, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1242128916261965930, articleId=1242128914085122150, tenantId=1146029695717560320, journalId=1146031591421210625, language=CN, title=西帕依固龈液对IL-1β刺激人牙龈成纤维细胞产生PGE₂的影响, columnId=1146540929516700224, journalTitle=科技导报, columnName=研究论文, runingTitle=null, highlight=null, articleAbstract=研究不同浓度西帕依固龈液对白细胞介素-1β(Interleukin-1β,IL-1β)刺激人牙龈成纤维细胞(Human Gingival Fibroblast,HGF)产生前列腺素E₂(Prostaglandin E₂,PGE₂)水平的影响。采用健康人牙龈组织原代培养的HGF细胞,以1ng/mL的IL-1β作为刺激因子,将HGF细胞按5×10⁴/mL的细胞密度接种到24孔培养板中,每孔200μL的细胞悬液。孵育24h贴壁后,弃原培养液,清洗2次后加药,分组为空白对照组(用含20mL/L的胎牛血清的DMEM培养液),IL-1β组(浓度为1ng/mL),IL-1β+西帕依固龈液组(西帕依固龈液终末浓度分别为12.5、25、50、100、200μg/mL),IL-1β+消炎痛(消炎痛浓度为100ng/mL)。用酶联免疫法测定PGE₂含量,观察不同浓度的西帕依固龈液(分别为12.5、25、50、100、200μg/mL)对HGF培养上清中PGE₂水平的影响。结果显示,5种不同浓度的西帕依固龈液均能显著抑制1ng/mL的IL-1β刺激后HGF产生PGE₂,随着浓度的增加,抑制效果也增加,但5种浓度的西帕依固龈液抑制效果均低于100ng/mL的消炎痛。由此得出,HGF具有合成和分泌PGE₂的功能,IL-1β能有效刺激HGF产生PGE₂;西帕依固龈液对IL-1β刺激HGF合成和分泌PGE₂具有显著抑制作用,其抑制作用在一定范围内呈浓度依赖性。, authors=艾比拜·玉素甫¹, 热娜古丽·艾则孜², 哈木拉提·吾甫尔², authorsList=艾比拜·玉素甫;热娜古丽·艾则孜;哈木拉提·吾甫尔, authorCompany=1. 新疆医科大学第一附属医院内分泌科,乌鲁木齐 830054;2. 新疆医科大学维吾尔医药系,乌鲁木齐 830054, correspAuthors=null, authorNote=null, correspAuthorsNote=哈木拉提·吾甫尔,教授,研究方向为新疆重大疾病的维吾尔医干预,电子信箱：Halmurat@263.net, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=7KwmoWgiwdQPIwJoKNX5Dw==, pdfFileSize=1522631, pdfExtLink=null, richHtmlUrl=null, 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