Article(id=1309634750638285202, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634747681304819, articleNumber=null, orderNo=null, doi=10.12048/j.issn.1674-229X.2026.01.003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1761667200000, receivedDateStr=2025-10-29, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790170856389, onlineDateStr=2026-09-23, pubDate=1769270400000, pubDateStr=2026-01-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790170856389, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790170856389, creator=13701087609, updateTime=1790170856389, updator=13701087609, issue=Issue{id=1309634747681304819, tenantId=1146029695717560320, journalId=1227999776411738115, year='2026', volume='36', issue='1', pageStart='1', pageEnd='80', issueExtLink='null', onlineDate='null', pubDate='1769270400000', pubDateStr='2026-01-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790170855683, creator='13701087609', updateTime=1790219109143, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309837137445482891, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634747681304819, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309837137445482892, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634747681304819, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=12, endPage=15, ext={EN=ArticleExt(id=1309634752236315027, articleId=1309634750638285202, tenantId=1146029695717560320, journalId=1227999776411738115, language=EN, title=A Method for Determining the Isoelectric Point of Human Basic Fibroblast Growth Factor, columnId=null, journalTitle=Pharmacy Today, columnName=null, runingTitle=null, highlight=null, articleAbstract=
OBJECTIVE

To establish an isoelectric focusing (IEF) analysis method for the physicochemical testing of human basic fibroblast growth factor (hbFGF).

METHODS

By comparing different IEF test conditions, the optimal method for determining the isoelectric point of hbFGF protein was screened, and the specificity, precision and durability of this method were methodologically verified.

RESULTS

The optimal system was the ampholyte system of Bio-Lyte and Ampholyte (2∶1). PBS buffer showed no interference with this method. The average pI value for repeatability was 10.0, with an RSD of 0.82% (≤3.0%). The average pI value for intermediate precision was 10.0, with a total RSD of 0.98% (≤6.0%). In terms of durability under different voltages (460, 480 and 500 V), the average pI value was 10.0, with an RSD of 1.3% (≤3.0%). In terms of durability under different electrophoresis times (70 min, 90 min, and 110 min), the average pI value was 10.0, with an RSD of 2.0% (≤3.0%).

CONCLUSION

The IEF method established for detecting the isoelectric point of hbFGF protein is accurate, durable and highly specific. It has high stability and can be used for the routine daily isoelectric point detection of hbFGF protein.

, authors=Junfang SHI1, 2, Yachao TAN2, Xiao FENG2, Wei XUE1, *, authorsList=Junfang SHI, Yachao TAN, Xiao FENG, Wei XUE, authorCompany=null, correspAuthors=Wei XUE, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309634752542499220, articleId=1309634750638285202, tenantId=1146029695717560320, journalId=1227999776411738115, language=CN, title=一种用于人碱性成纤维细胞生长因子等电点测定的方法, columnId=1228268589086606089, journalTitle=今日药学, columnName=论著, runingTitle=null, highlight=null, articleAbstract=
目的

建立一种用于检验人碱性成纤维细胞生长因子(hbFGF)理化检验的等电点聚焦电泳(IEF)分析方法。

方法

通过对比不同IEF法试验条件筛选针对hbFGF蛋白的等电点测定的最优方法,并对该方法的专属性、精密度及耐用性进行方法验证。

结果

两性电解质Bio-Lyte与Ampholyte(2∶1)体系为最优;PBS缓冲液不干扰该法;重复性的平均pI值为10.0,RSD为0.82(≤3.0%);中间精密度的平均pI值为10.0,总RSD为0.98%(≤6.0%);耐用性不同电压(460、480、500 V)的平均pI值为10.0,RSD为1.3%(≤3.0%);耐用性不同电泳时长(70 min、90 min、110 min)的平均pI值为10.0,RSD为2.0%(≤3.0%)。

结论

所建立的用于检测hbFGF蛋白等电点的IEF电泳法准确耐用,专属性强,稳定性高,可用于日常hbFGF蛋白的等电点检测。

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石俊芳,生物学博士,研究方向:生物制药

, correspAuthorsNote=

*

薛巍,教授,研究方向:医用高分子材料,E-mail:
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一种用于人碱性成纤维细胞生长因子等电点测定的方法
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石俊芳 1, 2 , 谭雅超 2 , 冯笑 2 , 薛巍 1, *
今日药学 | 论著 2026,36(1): 12-15
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今日药学 |论著 2026 , 36 (1) : 12 -15
一种用于人碱性成纤维细胞生长因子等电点测定的方法
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石俊芳1, 2, 谭雅超2, 冯笑2, 薛巍1, *
作者信息
  • 1.暨南大学生物医学工程系,广东 广州 510632
  • 2.朗肽生物制药股份有限公司,广东 佛山 528000
通讯作者:

*

薛巍,教授,研究方向:医用高分子材料,E-mail:
作者简介:

石俊芳,生物学博士,研究方向:生物制药

A Method for Determining the Isoelectric Point of Human Basic Fibroblast Growth Factor
Junfang SHI1, 2, Yachao TAN2, Xiao FENG2, Wei XUE1, *
Affiliations
  • 1.Jinan University School of Biomedical Engineering, Guangzhou, Guangdong 510632, China
  • 2.Langtai Biopharmaceutical Co., Ltd., Foshan, Guangdong 528000, China
出版时间: 2026-01-25 doi: 10.12048/j.issn.1674-229X.2026.01.003
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目的

建立一种用于检验人碱性成纤维细胞生长因子(hbFGF)理化检验的等电点聚焦电泳(IEF)分析方法。

方法

通过对比不同IEF法试验条件筛选针对hbFGF蛋白的等电点测定的最优方法,并对该方法的专属性、精密度及耐用性进行方法验证。

结果

两性电解质Bio-Lyte与Ampholyte(2∶1)体系为最优;PBS缓冲液不干扰该法;重复性的平均pI值为10.0,RSD为0.82(≤3.0%);中间精密度的平均pI值为10.0,总RSD为0.98%(≤6.0%);耐用性不同电压(460、480、500 V)的平均pI值为10.0,RSD为1.3%(≤3.0%);耐用性不同电泳时长(70 min、90 min、110 min)的平均pI值为10.0,RSD为2.0%(≤3.0%)。

结论

所建立的用于检测hbFGF蛋白等电点的IEF电泳法准确耐用,专属性强,稳定性高,可用于日常hbFGF蛋白的等电点检测。

人碱性成纤维细胞生长因子(hbFGF)  /  等电聚焦电泳法(IEF)  /  方法学验证
OBJECTIVE

To establish an isoelectric focusing (IEF) analysis method for the physicochemical testing of human basic fibroblast growth factor (hbFGF).

METHODS

By comparing different IEF test conditions, the optimal method for determining the isoelectric point of hbFGF protein was screened, and the specificity, precision and durability of this method were methodologically verified.

RESULTS

The optimal system was the ampholyte system of Bio-Lyte and Ampholyte (2∶1). PBS buffer showed no interference with this method. The average pI value for repeatability was 10.0, with an RSD of 0.82% (≤3.0%). The average pI value for intermediate precision was 10.0, with a total RSD of 0.98% (≤6.0%). In terms of durability under different voltages (460, 480 and 500 V), the average pI value was 10.0, with an RSD of 1.3% (≤3.0%). In terms of durability under different electrophoresis times (70 min, 90 min, and 110 min), the average pI value was 10.0, with an RSD of 2.0% (≤3.0%).

CONCLUSION

The IEF method established for detecting the isoelectric point of hbFGF protein is accurate, durable and highly specific. It has high stability and can be used for the routine daily isoelectric point detection of hbFGF protein.

human basic fibroblast growth factor (hbFGF)  /  isoelectric focusing electrophoresis (IEF)  /  methodological validation
石俊芳, 谭雅超, 冯笑, 薛巍. 一种用于人碱性成纤维细胞生长因子等电点测定的方法. 今日药学, 2026 , 36 (1) : 12 -15 . DOI: 10.12048/j.issn.1674-229X.2026.01.003
Junfang SHI, Yachao TAN, Xiao FENG, Wei XUE. A Method for Determining the Isoelectric Point of Human Basic Fibroblast Growth Factor[J]. Pharmacy Today, 2026 , 36 (1) : 12 -15 . DOI: 10.12048/j.issn.1674-229X.2026.01.003
  • 2022年佛山市南海区重点领域科技攻关专项(2230032004639)
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doi: 10.12048/j.issn.1674-229X.2026.01.003
  • 接收时间:2025-10-29
  • 首发时间:2026-09-23
  • 出版时间:2026-01-25
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  • 收稿日期:2025-10-29
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2022年佛山市南海区重点领域科技攻关专项(2230032004639)
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    1.暨南大学生物医学工程系,广东 广州 510632
    2.朗肽生物制药股份有限公司,广东 佛山 528000

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薛巍,教授,研究方向:医用高分子材料,E-mail:
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2种不同金属材料的力学参数
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species
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鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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