Article(id=1309634620182856341, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, articleNumber=null, orderNo=null, doi=10.12048/j.issn.1674-229X.2026.03.005, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1750262400000, receivedDateStr=2025-06-19, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790170825285, onlineDateStr=2026-09-23, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790170825285, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790170825285, creator=13701087609, updateTime=1790170825285, updator=13701087609, issue=Issue{id=1309634614356968036, tenantId=1146029695717560320, journalId=1227999776411738115, year='2026', volume='36', issue='3', pageStart='161', pageEnd='240', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790170823897, creator='13701087609', updateTime=1790219059643, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309836929789682362, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309836929789682363, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=185, endPage=190, ext={EN=ArticleExt(id=1309634620363211414, articleId=1309634620182856341, tenantId=1146029695717560320, journalId=1227999776411738115, language=EN, title=Uncertainty Analysis of Serum Vortioxetine Concentration in Patients with Mental Disorders Based on LC-MS/MS Method, columnId=null, journalTitle=Pharmacy Today, columnName=null, runingTitle=null, highlight=null, articleAbstract=
OBJECTIVE

To assess uncertainty in the determination of vortioxetine concentrations in serum from patients with psychiatric disorders using HPLC-MS/MS methodology.

METHODS

For the determination of vortioxetine concentration in human serum, the uncertainty introduced by repeated measurements was evaluated using the type A evaluation. The uncertainties contributed from standard weighing, purity, solution preparation, preparation of drug-containing serum, extraction recovery, matrix effect, calibration curve fitting and instrumental precision were assessed using the type B evaluation. Subsequently, the synthetic and extended uncertainties were calculated.

RESULTS

The extended uncertainties for low (0.6 ng·mL-1), medium (60 ng·mL-1), and high (120 ng·mL-1) concentrations of vortioxetine in human serum were 0.67, 4.10, and 10.49 ng·mL-1, respectively, when confidence probability was P = 95% (k = 2).

CONCLUSION

The method employed in this study exhibits high accuracy and is suitable for the therapeutic drug monitoring of vortioxetine. The primary source of uncertainty arises from the calibration curve fitting, with particularly elevated uncertainty values introduced at the low concentration (0.6 ng·mL -1). Implementing a rational linear range and optimizing calibration curve fitting can enhance the accuracy of measurement results.

, authors=Yongyu DING1, Lijing DAI2, Guilan WU1, Yi ZOU1, Yuqing CHEN2, Yaoqi WEN2, Zhenlin WANG2, Yuguan WEN2, *, authorsList=Yongyu DING, Lijing DAI, Guilan WU, Yi ZOU, Yuqing CHEN, Yaoqi WEN, Zhenlin WANG, Yuguan WEN, authorCompany=null, correspAuthors=Yuguan WEN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309634620702950039, articleId=1309634620182856341, tenantId=1146029695717560320, journalId=1227999776411738115, language=CN, title=基于LC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度分析, columnId=1228268589086606089, journalTitle=今日药学, columnName=论著, runingTitle=null, highlight=null, articleAbstract=
目的

分析基于HPLC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度。

方法

测定人血清中伏硫西汀浓度,采用A类评定法计算重复性测量引入的不确定度,采用B类评定法计算标准品称量、纯度、溶液配制、含药血清配制、提取回收率、基质效应、标曲拟合以及仪器测定引入的不确定度,并计算合成和扩展不确定度。

结果

当P=95%置信概率(k=2)时,人血清中低(0.6 ng·mL-1)、中(60 ng·mL-1)、高(120 ng·mL-1)浓度伏硫西汀的扩展不确定度分别为0.67、4.10和10.49 ng·mL-1。

结论

本研究方法准确度高,适用于伏硫西汀的血药浓度监测。不确定度主要由标曲拟合引入,低浓度时标曲拟合引入的不确定度值较高。设定合理的线性范围可提高测定结果准确性。

, authors=丁勇宇1, 戴丽静2, 巫贵兰1, 邹祎1, 陈玉清2, 温耀祺2, 王震霖2, 温预关2, *, authorsList=丁勇宇, 戴丽静, 巫贵兰, 邹祎, 陈玉清, 温耀祺, 王震霖, 温预关, authorCompany=null, correspAuthors=温预关, authorNote=

丁勇宇,主管药师,研究方向:医院药学个体化药学服务

, correspAuthorsNote=

*

温预关,主任药师,研究方向:个体化用药及药代动力学研究,E-mail:
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丁勇宇,主管药师,研究方向:医院药学个体化药学服务

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基于LC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度分析
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丁勇宇 1 , 戴丽静 2 , 巫贵兰 1 , 邹祎 1 , 陈玉清 2 , 温耀祺 2 , 王震霖 2 , 温预关 2, *
今日药学 | 论著 2026,36(3): 185-190
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今日药学 |论著 2026 , 36 (3) : 185 -190
基于LC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度分析
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丁勇宇,主管药师,研究方向:医院药学个体化药学服务

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丁勇宇1, 戴丽静2, 巫贵兰1, 邹祎1, 陈玉清2, 温耀祺2, 王震霖2, 温预关2, *
作者信息
  • 1.惠州市第二人民医院药学部,广东 惠州 516001
  • 2.广州医科大学附属脑科医院药学部,广东 广州 510370
通讯作者:

*

温预关,主任药师,研究方向:个体化用药及药代动力学研究,E-mail:
作者简介:

丁勇宇,主管药师,研究方向:医院药学个体化药学服务

Uncertainty Analysis of Serum Vortioxetine Concentration in Patients with Mental Disorders Based on LC-MS/MS Method
Yongyu DING1, Lijing DAI2, Guilan WU1, Yi ZOU1, Yuqing CHEN2, Yaoqi WEN2, Zhenlin WANG2, Yuguan WEN2, *
Affiliations
  • 1.Department of Pharmacy, Huizhou Second People's Hospital, Huizhou, Guangdong 516001, China
  • 2.Department of Pharmacy, The Affiliated Brain Hospital, Guangzhou Medical University, Guangzhou, Guangdong 510370, China
出版时间: 2026-03-25 doi: 10.12048/j.issn.1674-229X.2026.03.005
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目的

分析基于HPLC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度。

方法

测定人血清中伏硫西汀浓度,采用A类评定法计算重复性测量引入的不确定度,采用B类评定法计算标准品称量、纯度、溶液配制、含药血清配制、提取回收率、基质效应、标曲拟合以及仪器测定引入的不确定度,并计算合成和扩展不确定度。

结果

当P=95%置信概率(k=2)时,人血清中低(0.6 ng·mL-1)、中(60 ng·mL-1)、高(120 ng·mL-1)浓度伏硫西汀的扩展不确定度分别为0.67、4.10和10.49 ng·mL-1。

结论

本研究方法准确度高,适用于伏硫西汀的血药浓度监测。不确定度主要由标曲拟合引入,低浓度时标曲拟合引入的不确定度值较高。设定合理的线性范围可提高测定结果准确性。

伏硫西汀  /  不确定度  /  HPLC-MS/MS  /  血清药物浓度
OBJECTIVE

To assess uncertainty in the determination of vortioxetine concentrations in serum from patients with psychiatric disorders using HPLC-MS/MS methodology.

METHODS

For the determination of vortioxetine concentration in human serum, the uncertainty introduced by repeated measurements was evaluated using the type A evaluation. The uncertainties contributed from standard weighing, purity, solution preparation, preparation of drug-containing serum, extraction recovery, matrix effect, calibration curve fitting and instrumental precision were assessed using the type B evaluation. Subsequently, the synthetic and extended uncertainties were calculated.

RESULTS

The extended uncertainties for low (0.6 ng·mL-1), medium (60 ng·mL-1), and high (120 ng·mL-1) concentrations of vortioxetine in human serum were 0.67, 4.10, and 10.49 ng·mL-1, respectively, when confidence probability was P = 95% (k = 2).

CONCLUSION

The method employed in this study exhibits high accuracy and is suitable for the therapeutic drug monitoring of vortioxetine. The primary source of uncertainty arises from the calibration curve fitting, with particularly elevated uncertainty values introduced at the low concentration (0.6 ng·mL -1). Implementing a rational linear range and optimizing calibration curve fitting can enhance the accuracy of measurement results.

vortioxetine  /  uncertainty  /  HPLC-MS/MS  /  serum drug concentration
丁勇宇, 戴丽静, 巫贵兰, 邹祎, 陈玉清, 温耀祺, 王震霖, 温预关. 基于LC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度分析. 今日药学, 2026 , 36 (3) : 185 -190 . DOI: 10.12048/j.issn.1674-229X.2026.03.005
Yongyu DING, Lijing DAI, Guilan WU, Yi ZOU, Yuqing CHEN, Yaoqi WEN, Zhenlin WANG, Yuguan WEN. Uncertainty Analysis of Serum Vortioxetine Concentration in Patients with Mental Disorders Based on LC-MS/MS Method[J]. Pharmacy Today, 2026 , 36 (3) : 185 -190 . DOI: 10.12048/j.issn.1674-229X.2026.03.005
  • 广东省药学会科学研究基金(2024A34)
  • 2024年惠州市医疗卫生领域科技计划项目(2024CZ010140)
  • 中华国际医学交流基金会医药临床与成果转化基金-临床药学发展专项基金项目(Z-2014-08-2309-5)
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doi: 10.12048/j.issn.1674-229X.2026.03.005
  • 接收时间:2025-06-19
  • 首发时间:2026-09-23
  • 出版时间:2026-03-25
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出版历史
  • 收稿日期:2025-06-19
基金
广东省药学会科学研究基金(2024A34)
2024年惠州市医疗卫生领域科技计划项目(2024CZ010140)
中华国际医学交流基金会医药临床与成果转化基金-临床药学发展专项基金项目(Z-2014-08-2309-5)
作者信息
    1.惠州市第二人民医院药学部,广东 惠州 516001
    2.广州医科大学附属脑科医院药学部,广东 广州 510370

通讯作者:

*

温预关,主任药师,研究方向:个体化用药及药代动力学研究,E-mail:
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https://castjournals.cast.org.cn/joweb/jryx/CN/10.12048/j.issn.1674-229X.2026.03.005
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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