Article(id=1309634587958014013, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634582362809158, articleNumber=null, orderNo=null, doi=10.12048/j.issn.1674-229X.2026.04.005, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1749484800000, receivedDateStr=2025-06-10, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790170817602, onlineDateStr=2026-09-23, pubDate=1777046400000, pubDateStr=2026-04-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790170817602, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790170817602, creator=13701087609, updateTime=1790170817602, updator=13701087609, issue=Issue{id=1309634582362809158, tenantId=1146029695717560320, journalId=1227999776411738115, year='2026', volume='36', issue='4', pageStart='241', pageEnd='320', issueExtLink='null', onlineDate='null', pubDate='1777046400000', pubDateStr='2026-04-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790170816268, creator='13701087609', updateTime=1790219015105, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309836742979576497, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634582362809158, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309836742979576498, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634582362809158, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=276, endPage=281, ext={EN=ArticleExt(id=1309634588121591870, articleId=1309634587958014013, tenantId=1146029695717560320, journalId=1227999776411738115, language=EN, title=Simultaneous Determination of Eight Components in
Qiju Dihuang Condensed Pills by UPLC-DAD, columnId=null, journalTitle=Pharmacy Today, columnName=null, runingTitle=null, highlight=null, articleAbstract=
OBJECTIVE To establish an ultra-performance liquid chromatography-diode array detector (UPLC-DAD) method for the simultaneous determination of 5-hydroxymethylfurfural, morroniside, chlorogenic acid, loganin, paeoniflorin, 4, 5-O-dicaffeoylquinic acid, benzoylpaeoniflorin, and paeonol in Qiju Dihuang condensed pills.
METHODS Separation was performed on a Waters ACQUITY HSS T3 C18 column (2.1 mm × 100 mm, 1.8 μm) using a gradient elution program consisting of methanol, acetonitrile, and 0.3% phosphoric acid. Detection wavelengths were set at 230 nm (paeoniflorin, benzoylpaeoniflorin), 240 nm (morroniside, loganin), 274 nm (paeonol), 284 nm (5-hydroxymethylfurfural), 325 nm (chlorogenic acid), and 330 nm (4, 5-O-dicaffeoylquinic acid). The column temperature was maintained at 35 ℃， with a flow rate of 0.3 mL·min -1 and an injection volume of 1 μL.
RESULTS The calibration curves for 5-hydroxymethylfurfural, morroniside, chlorogenic acid, loganin, paeoniflorin, 4, 5-O-dicaffeoylquinic acid, benzoylpaeoniflorin, and paeonol exhibited good linearity within concentration ranges of 4.69-234.53 (r = 0.999 3), 2.43-121.52 (r=0.999 8), 1.12-56.09 (r = 0.999 9), 2.81-140.33 (r = 0.999 9), 2.55-127.64 (r = 0.999 8), 1.17-58.60 (r = 0.999 8), 2.22-110.75 (r=0.999 5), and 4.93-246.25 μg·mL-1 (r = 0.999 9), respectively. The average recoveries were 99.67%, 99.30%, 97.64%, 99.88%, 99.90%, 102.60%, 99.65%, 100.59% with corresponding RSDs of 0.97%, 1.36%, 0.75%, 1.03%, 1.72%, 0.64%, 1.01% and 1.00%.
CONCLUSION The developed method is rapid, accurate, sensitive, efficient, and reproducible, making it suitable for the determination of multi-index components, quality control and quality evaluation of Qiju Dihuang condensed pill.
, authors=Chengshuai WANG, Zhan SHU, Lanjun ZHENG, Jiaqi WU, authorsList=Chengshuai WANG, Zhan SHU, Lanjun ZHENG, Jiaqi WU, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309634588431970367, articleId=1309634587958014013, tenantId=1146029695717560320, journalId=1227999776411738115, language=CN, title=UPLC-DAD法同时测定杞菊地黄丸中8种成分含量, columnId=1228268589086606089, journalTitle=今日药学, columnName=论著, runingTitle=null, highlight=null, articleAbstract=
目的 建立超高效液相色谱-二极管阵列检测器法（UPLC-DAD）同时测定杞菊地黄丸（浓缩丸）中5-羟甲基糠醛、莫诺苷、绿原酸、马钱苷、芍药苷、4，5-O-二咖啡酰奎宁酸、苯甲酰芍药苷、丹皮酚的含量。
方法 采用Waters ACQUITY HSS T3 C18色谱柱（2.1 mm × 100 mm，1.8 μm）；以甲醇-乙腈-0.3%磷酸为流动相梯度程序洗脱；检测波长：230 nm（芍药苷、苯甲酰芍药苷）、240 nm（莫诺苷、马钱苷）、274 nm（丹皮酚）、284 nm（5-羟甲基糠醛）、325 nm（绿原酸）、330 nm（4，5-O-二咖啡酰奎宁酸）；柱温为35℃；流速为0.3 mL·min–1；进样量为1 μL。
结果 8种指标成分5-羟甲基糠醛、莫诺苷、绿原酸、马钱苷、芍药苷、4，5-O-二咖啡酰奎宁酸、苯甲酰芍药苷、丹皮酚分别在4.69 ~ 234.53（r = 0.9993）、2.43 ~ 121.52（r = 0.9998）、1.12 ~ 56.09（r = 0.9999）、2.81 ~140.33（r = 0.9999）、2.55 ~ 127.64（r = 0.9998）、1.17 ~ 58.60（r = 0.9998）、2.22 ~ 110.75（r = 0.9995）、4.93 ~ 246.25 μg·mL–1（r = 0.9999）浓度范围内线性关系良好，平均加样回收率分别为99.67%、99.30%、97.64%、99.88%、99.90%、102.60%、99.65%、100.59%，RSD分别为0.97%、1.36%、0.75%、1.03%、1.72%、0.64%、1.01%、1.00%。
结论 该方法简便、快速准确、重复性好，可用于杞菊地黄丸（浓缩丸）多指标成分测定及质量控制和评价。
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