今日药学

癌因性疲乏小鼠疲乏特点探索研究

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叶小榕 ¹ , 温玉莹 ² , 张文强 ² , 黄远铿 ² , 代彩玲 ² , 郭健敏 ²^,^*

今日药学 | 论著 2026,36(6): 421-427

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今日药学 |论著 2026 , 36 (6) : 421 -427

癌因性疲乏小鼠疲乏特点探索研究

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叶小榕¹, 温玉莹², 张文强², 黄远铿², 代彩玲², 郭健敏²^,^*

作者信息

^1．四川省绵阳市中医医院，四川绵阳 621000

^2．广州湾区生物医药研究院，广东莱恩医药研究院有限公司，广东省药物非临床评价与研究重点实验室，国家中药现代化工程技术研究中心中药非临床评价分中心，广东省创新药物评价与研究工程技术研究中心，从化区抗感染药物研究与评价工程技术研究中心，广东广州 510990

通讯作者:

郭健敏，硕士研究生导师，正高级工程师，研究方向：新药药理毒理，E-mail: guojianmin@lewwin.com.cn

作者简介:

叶小榕，副主任医师，研究方向：儿内科临床工作

Exploration and Study on the Fatigue Characteristics of Cancer-Related Fatigue Mice

Xiaorong YE¹, Yuying WEN², Wenqiang ZHANG², Yuankeng HUANG², Cailing DAI², Jianmin GUO²^,^*

Affiliations

^1.Mianyang Hospital of Traditional Chinese Medicine, Mianyang, Sichuan 621000, China

^2.Guangzhou Bay Area Institute of Biomedicine, Guangdong Lewwin Pharmaceutical Research Institute Co., Ltd., Guangdong Provincial Key Laboratory of Drug Non-Clinical Evaluation and Research, Non-clinical Evaluation Sub-center for Traditional Chinese Medicine of National Engineering Research Center for Modernization of Traditional Chinese Medicine, Guangdong Engineering Research Center for Innovative Drug Evaluation and Research, Conghua District Anti-infective Drug Research and Evaluation Engineering and Technology Research Center, Guangzhou, Guangdong 510990, China

出版时间: 2026-06-25 doi: 10.12048/j.issn.1674-229X.2026.06.004

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摘要

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目的

癌因性疲乏（cancer-related fatigue, CRF）是肿瘤患者最常见的症状之一，本研究通过建立Lewis肺癌、CT-26结肠癌和WEHI-3白血病小鼠CRF模型，探索CRF的动态特点及药物治疗评价指标。

方法

以C57BL/6小鼠和BALB/c小鼠为研究对象，分别采用小鼠Lewis肺癌细胞、小鼠CT-26结肠癌细胞、小鼠WEHI-3白血病细胞来诱导CRF模型，通过转棒试验检测小鼠造模后运动耐力的变化，ELISA检测小鼠造模后血清中尿素氮（BUN）、肌酸激酶（CK）、促红细胞生成素（EPO）、白介素1（IL-1）、肿瘤坏死因子α（TNF-α）、超敏C反应蛋白（hs-CRP）含量变化和骨骼肌中超氧化物岐化酶（SOD）和乳酸脱氢酶（LDH）含量变化。

结果

3种模型小鼠在造模后均出现被毛蓬松、弓背、活动减少、行动迟缓等症状，运动耐力均显著降低。Lewis肺癌、CT-26结肠癌、WEHI-3白血病模型小鼠在肿瘤发展后期运动耐力下降幅度均变大，分别在造模后第17天、第28天、第19天血清中BUN含量均显著下降，CK含量均显著升高。WEHI-3白血病模型小鼠在造模后第19天血清中TNF-α显著升高，CT-26结肠癌模型小鼠在造模后第28天骨骼肌中LDH含量显著降低，血清中IL-1、TNF-α、hs-CRP含量具有升高的趋势。

结论

Lewis肺癌、CT-26结肠癌、WEHI-3白血病小鼠模型，可用于观察由肿瘤引起的疲劳行为。转棒时间变化、血清中CK、BUN、IL-1、TNF-α、hs-CRP以及骨骼肌中LDH等指标可作为非临床研究中CRF药物疗效评价指标。

关键词

癌因性疲乏 / 荷瘤模型 / 转棒试验 / 疲乏相关因子

Abstract

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OBJECTIVE

Cancer-related fatigue (CRF) is one of the most common symptoms in cancer patients. In this study, CRF models of Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia mice were established to investigate the dynamic characteristics of CRF and explore evaluation indicators for drug treatment of CRF.

METHODS

C57BL/6 mice and BALB/c mice were inoculated with Lewis lung cancer cells, CT-26 colon cancer cells and WEHI-3 leukemia cells to induce CRF models. The rotarod test was employed to assess changes in the exercise endurance of the mice following model induction. ELISA was used to detect the changes in the contents of urea nitrogen (BUN), creatine kinase (CK), erythropoietin (EPO), interleukin-1 (IL-1), tumor necrosis factor-α (TNF-α), and high-sensitivity C-reactive protein (hs-CRP) in the serum of mice post-modeling, as well as the levels of superoxide dismutase (SOD) and lactate dehydrogenase (LDH) in skeletal muscle.

RESULTS

All three types of model mice showed symptoms such as fluffy coat, hunched back, reduced activity and slow movement after modeling. Their exercise endurance was significantly reduced. The decrease in exercise endurance among Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia model mice increased during the later stage of tumor development. The BUN content in serum decreased significantly on days 17, 28 and 19 post-modeling respectively. Meanwhile the CK content increased significantly. TNF-α levels in serum from the WEHI-3 leukemia model mice significantly increased on day 19 post-modeling. The LDH content within skeletal muscle from CT-26 colon cancer model mice significantly decreased on day 28 post-modeling, and the contents of IL-1, TNF-α and hs-CRP in the serum showed an increasing trend.

CONCLUSION

Mouse models of Lewis lung cancer, CT-26 colon cancer and WEHI-3 leukemia can be utilized to observe fatigue behavior induced by tumors. Changes in rotarod test time as well as serum CK, BUN, IL-1, TNF-α, hs-CRP and skeletal muscle LDH may serve effectively as evaluation indicators for the efficacy of CRF therapeutic drugs in non-clinical studies.

Key words

cancer-related fatigue (CRF) / tumor-bearing model / rotarod test / fatigue-related factors

引用本文

叶小榕, 温玉莹, 张文强, 黄远铿, 代彩玲, 郭健敏. 癌因性疲乏小鼠疲乏特点探索研究. 今日药学, 2026 , 36 (6) : 421 -427 . DOI: 10.12048/j.issn.1674-229X.2026.06.004

Xiaorong YE, Yuying WEN, Wenqiang ZHANG, Yuankeng HUANG, Cailing DAI, Jianmin GUO. Exploration and Study on the Fatigue Characteristics of Cancer-Related Fatigue Mice[J]. Pharmacy Today, 2026 , 36 (6) : 421 -427 . DOI: 10.12048/j.issn.1674-229X.2026.06.004

基金

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广东省科技计划项目(2023B1212070029)
“广东特支计划”科技创业领军人才(2021TY060021)
科技创业领军人才(SQ2024RA3E000198)

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2026年第36卷第6期

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文章信息

doi: 10.12048/j.issn.1674-229X.2026.06.004

接收时间：2025-07-11
首发时间：2026-09-23
出版时间：2026-06-25

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收稿日期：2025-07-11

基金

广东省科技计划项目(2023B1212070029)

“广东特支计划”科技创业领军人才(2021TY060021)

科技创业领军人才(SQ2024RA3E000198)

作者信息

^1．四川省绵阳市中医医院，四川绵阳 621000

通讯作者:

郭健敏，硕士研究生导师，正高级工程师，研究方向：新药药理毒理，E-mail: guojianmin@lewwin.com.cn

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https://castjournals.cast.org.cn/joweb/jryx/CN/10.12048/j.issn.1674-229X.2026.06.004

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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