Article(id=1309634620182856341, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, articleNumber=null, orderNo=null, doi=10.12048/j.issn.1674-229X.2026.03.005, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1750262400000, receivedDateStr=2025-06-19, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790170825285, onlineDateStr=2026-09-23, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790170825285, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790170825285, creator=13701087609, updateTime=1790170825285, updator=13701087609, issue=Issue{id=1309634614356968036, tenantId=1146029695717560320, journalId=1227999776411738115, year='2026', volume='36', issue='3', pageStart='161', pageEnd='240', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790170823897, creator='13701087609', updateTime=1790219059643, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309836929789682362, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309836929789682363, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=185, endPage=190, ext={EN=ArticleExt(id=1309634620363211414, articleId=1309634620182856341, tenantId=1146029695717560320, journalId=1227999776411738115, language=EN, title=Uncertainty Analysis of Serum Vortioxetine Concentration in Patients with Mental Disorders Based on LC-MS/MS Method, columnId=null, journalTitle=Pharmacy Today, columnName=null, runingTitle=null, highlight=null, articleAbstract=
OBJECTIVE To assess uncertainty in the determination of vortioxetine concentrations in serum from patients with psychiatric disorders using HPLC-MS/MS methodology.
METHODS For the determination of vortioxetine concentration in human serum, the uncertainty introduced by repeated measurements was evaluated using the type A evaluation. The uncertainties contributed from standard weighing, purity, solution preparation, preparation of drug-containing serum, extraction recovery, matrix effect, calibration curve fitting and instrumental precision were assessed using the type B evaluation. Subsequently, the synthetic and extended uncertainties were calculated.
RESULTS The extended uncertainties for low (0.6 ng·mL-1), medium (60 ng·mL-1), and high (120 ng·mL-1) concentrations of vortioxetine in human serum were 0.67, 4.10, and 10.49 ng·mL-1, respectively, when confidence probability was P = 95% (k = 2).
CONCLUSION The method employed in this study exhibits high accuracy and is suitable for the therapeutic drug monitoring of vortioxetine. The primary source of uncertainty arises from the calibration curve fitting, with particularly elevated uncertainty values introduced at the low concentration (0.6 ng·mL -1). Implementing a rational linear range and optimizing calibration curve fitting can enhance the accuracy of measurement results.
, authors=Yongyu DING
1, Lijing DAI
2, Guilan WU
1, Yi ZOU
1, Yuqing CHEN
2, Yaoqi WEN
2, Zhenlin WANG
2, Yuguan WEN
2, *, authorsList=Yongyu DING, Lijing DAI, Guilan WU, Yi ZOU, Yuqing CHEN, Yaoqi WEN, Zhenlin WANG, Yuguan WEN, authorCompany=null, correspAuthors=Yuguan WEN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1309634620702950039, articleId=1309634620182856341, tenantId=1146029695717560320, journalId=1227999776411738115, language=CN, title=基于LC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度分析, columnId=1228268589086606089, journalTitle=今日药学, columnName=论著, runingTitle=null, highlight=null, articleAbstract=
目的 分析基于HPLC-MS/MS法测定精神障碍患者血清中伏硫西汀浓度的不确定度。
方法 测定人血清中伏硫西汀浓度,采用A类评定法计算重复性测量引入的不确定度,采用B类评定法计算标准品称量、纯度、溶液配制、含药血清配制、提取回收率、基质效应、标曲拟合以及仪器测定引入的不确定度,并计算合成和扩展不确定度。
结果 当P=95%置信概率(k=2)时,人血清中低(0.6 ng·mL-1)、中(60 ng·mL-1)、高(120 ng·mL-1)浓度伏硫西汀的扩展不确定度分别为0.67、4.10和10.49 ng·mL-1。
结论 本研究方法准确度高,适用于伏硫西汀的血药浓度监测。不确定度主要由标曲拟合引入,低浓度时标曲拟合引入的不确定度值较高。设定合理的线性范围可提高测定结果准确性。
, authors=丁勇宇
1, 戴丽静
2, 巫贵兰
1, 邹祎
1, 陈玉清
2, 温耀祺
2, 王震霖
2, 温预关
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丁勇宇,主管药师,研究方向:医院药学个体化药学服务
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