Article(id=1309634628969914522, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, articleNumber=null, orderNo=null, doi=10.12048/j.issn.1674-229X.2026.03.002, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1752508800000, receivedDateStr=2025-07-15, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790170827380, onlineDateStr=2026-09-23, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790170827380, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790170827380, creator=13701087609, updateTime=1790170827380, updator=13701087609, issue=Issue{id=1309634614356968036, tenantId=1146029695717560320, journalId=1227999776411738115, year='2026', volume='36', issue='3', pageStart='161', pageEnd='240', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790170823897, creator='13701087609', updateTime=1790219059643, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309836929789682362, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309836929789682363, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634614356968036, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=171, endPage=174, ext={EN=ArticleExt(id=1309634629120909467, articleId=1309634628969914522, tenantId=1146029695717560320, journalId=1227999776411738115, language=EN, title=Exploration of Allergy Testing Method for Cattle Encephalon Glycoside and Ignotin Injection Based on Mouse Popliteal Lymph Node Model, columnId=null, journalTitle=Pharmacy Today, columnName=null, runingTitle=null, highlight=null, articleAbstract=
OBJECTIVE To improve the allergic reaction test method of Cattle Encephalon Glycoside and Ignotin Injection by using the direct popliteal lymph node (D-PLNA) and reporter antigen popliteal lymph node (RA-PLNA) models in mice.
METHODS In the D-PLNA model, the Cattle Encephalon Glycoside and Ignotin Injection group, normal saline control group and positive drug D-penicillamine hydrochloride control group were set up. In the RA-PLNA model, the above groups were respectively added with reporter antigens TNP-Ficoll and TNP-OVA, and 50 μL of the drug was administered to the left toe pad. On the 7th day, the mice were sacrificed, and the popliteal lymph nodes were isolated, ground to prepare lymphocyte single-cell suspensions and counted to calculate the cell index. The cell suspension was cultured in 96-well plates, and after co-incubation with stimulators, the contents of IgE and MCP-1 in the cell supernatant were determined by ELISA.
RESULTS Compared with the normal saline control group, in the RA-PLNA model of mice, the number of cells in the popliteal lymph nodes on the drug administration side increased in the group added with TNP-OVA (P < 0.05), and the contents of IgE and MCP-1 both increased (P < 0.05).
CONCLUSION Cattle encephalon glycoside and ignotin injection only caused a positive lymph node reaction in mice when TNP-OVA was added, indicating that there may be small molecule substances in Cattle Encephalon Glycoside and Ignotin Injection that cooperate with TNP-OVA to induce allergic reactions.
, authors=Wei SHEN, Yibo LIANG, Nan XIE
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目的 利用小鼠直接腘窝淋巴结（D-PLNA）和报告抗原腘窝淋巴结（RA-PLNA）模型对脑苷肌肽注射液的过敏检查方法进行改进，以寻求新的针对该品种更敏感、更准确的检查方法。
方法 D-PLNA模型中设脑苷肌肽注射液组，生理盐水对照组和阳性药物盐酸D-青霉胺对照组；RA-PLNA模型中上述各组分别加入报告抗原TNP-Ficoll和TNP-OVA，于左足趾部给药50 μL。第7天处死小鼠分离腘窝淋巴结，研磨制备淋巴单细胞悬液并进行细胞计数，计算细胞指数；将细胞悬液培养于96孔板中，加入刺激剂共孵育后，利用ELISA技术测定细胞上清液中IgE和MCP-1的含量。
结果 与生理盐水对照组比较，小鼠RA-PLNA模型中，加入TNP-OVA组的给药侧腘窝淋巴结细胞数增加（P<0.05），IgE与MCP-1含量均升高（P<0.05）。
结论 脑苷肌肽注射液仅在加入报告抗原TNP-OVA后引起小鼠淋巴结阳性反应，说明脑苷肌肽注射液中可能存在小分子物质与TNP-OVA协同诱发过敏反应。
, authors=沈薇, 梁一博, 谢楠
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