Article(id=1309634772016652864, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634747681304819, articleNumber=null, orderNo=null, doi=10.12048/j.issn.1674-229X.2026.01.002, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1754928000000, receivedDateStr=2025-08-12, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1790170861485, onlineDateStr=2026-09-23, pubDate=1769270400000, pubDateStr=2026-01-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1790170861485, onlineIssueDateStr=2026-09-23, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1790170861485, creator=13701087609, updateTime=1790170861485, updator=13701087609, issue=Issue{id=1309634747681304819, tenantId=1146029695717560320, journalId=1227999776411738115, year='2026', volume='36', issue='1', pageStart='1', pageEnd='80', issueExtLink='null', onlineDate='null', pubDate='1769270400000', pubDateStr='2026-01-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1790170855683, creator='13701087609', updateTime=1790219109143, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1309837137445482891, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634747681304819, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1309837137445482892, tenantId=1146029695717560320, journalId=1227999776411738115, issueId=1309634747681304819, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=6, endPage=11, ext={EN=ArticleExt(id=1309634772436083267, articleId=1309634772016652864, tenantId=1146029695717560320, journalId=1227999776411738115, language=EN, title=The Impact of Active Probiotics on Promoting Lead Excretion and Relieving Constipation, columnId=null, journalTitle=Pharmacy Today, columnName=null, runingTitle=null, highlight=null, articleAbstract=
OBJECTIVE To investigate the effects of active liquid probiotics on the laxative efficacy and promotion of lead excretion in zebrafish.
METHODS Zebrafish at 5 dpf (days post fertilization) were stained with Nile Red and then divided into 3 groups, treated at 28 °C for 1 day, and their intestinal fluorescence intensity was analyzed. Zebrafish at 6 hpf (hours post fertilization) were used to establish a lead poisoning model, treated at 28 ℃ for 4 days, and then the lead content in vivo was determined.
RESULTS The maximum tested concentration (MTC) of active liquid probiotics for laxative effect in zebrafish was 15.6 µL·mL-1. At this concentration, the intestinal fluorescence intensity of the probiotic group was significantly reduced (compared with the normal control group, P < 0.001). Similarly, for its effect on promoting lead excretion in zebrafish, the MTC was 7.81 µL·mL-1, and the lead content in the probiotic group at this concentration decreased by approximately 50% compared with the model group (P < 0.001).
CONCLUSION Active liquid probiotics can rapidly colonize the zebrafish intestine and promote the proliferation of beneficial flora, thereby achieving laxative effects and regulating intestinal vitality. Active liquid probiotics can utilize their unique ion-binding capacity to specifically adsorb heavy metal lead for excretion, enhancing lead excretion.
, authors=Zhili YAN
1, Xinyue ZHANG
2, Wenqing CHEN
1, Yiju WEI
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目的 探讨活性液体益生菌对斑马鱼润肠通便功效以及促进其体内排铅作用的影响。
方法 将5 dpf（days post fertilization，受精后天数）斑马鱼经尼罗红染色后分为3组，28 ℃处理1 d，分析肠道荧光强度；将6 hpf（hours post fertilization，受精后小时数）斑马鱼建立铅中毒模型，28 ℃处理4 d后检测体内铅含量。
结果 活性液体益生菌对润肠通便功效斑马鱼最大检测浓度（maximum tested concentration, MTC）为15.6 µL·mL-1，此浓度的益生菌组肠道荧光强度显著降低（与正常对照组比较，P<0.001）对促进排铅功效斑马鱼MTC为7.81 µL·mL-1，此浓度的益生菌组体内铅含量较模型组下降约50%（P<0.001）。
结论 活性液体益生菌能够快速定植肠道，促进有益菌群增殖，从而实现润肠通便、调节肠道活力；活性液体益生菌可以利用独特离子结合能力，靶向吸附并排出重金属铅，促进排铅功效。
, authors=严致立
1, 张馨月
2, 陈文清
1, 魏义举
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严致立，在读硕士研究生，研究方向：益生菌调控肠道细胞死亡与营养干预
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