首页 期刊 论文 学科刊群 资讯 加盟 关于
EN
image
Article(id=1244313109855318302, tenantId=1146029695717560320, journalId=1149651085930835976, issueId=1244313103459008874, articleNumber=null, orderNo=null, doi=10.3969/j.issn.0253-4193.2020.12.008, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=research-article, receivedDate=1569168000000, receivedDateStr=2019-09-23, revisedDate=1578412800000, revisedDateStr=2020-01-08, acceptedDate=null, acceptedDateStr=null, onlineDate=1774596963608, onlineDateStr=2026-03-27, pubDate=1608825600000, pubDateStr=2020-12-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1774596963608, onlineIssueDateStr=2026-03-27, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1774596963608, creator=13701087609, updateTime=1774596963608, updator=13701087609, issue=Issue{id=1244313103459008874, tenantId=1146029695717560320, journalId=1149651085930835976, year='2020', volume='42', issue='12', pageStart='1', pageEnd='128', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1774596962084, creator=13701087609, updateTime=1774597044552, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1244313449409393475, tenantId=1146029695717560320, journalId=1149651085930835976, issueId=1244313103459008874, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1244313449409393476, tenantId=1146029695717560320, journalId=1149651085930835976, issueId=1244313103459008874, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=72, endPage=81, ext={EN=ArticleExt(id=1244313111537234253, articleId=1244313109855318302, tenantId=1146029695717560320, journalId=1149651085930835976, language=EN, title=Distribution of betaine lipids in 14 species of microalgae, columnId=1194652705852465724, journalTitle=Haiyang Xuebao, columnName=Article, runingTitle=null, highlight=null, articleAbstract=

The qualitative and quantitative analyses on betaine lipids of microalgae were conducted by means of the quadrupole flight time ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-Q-TOF-MS) using electron spraying ionization. As a result, 133 betaine lipids including 53 DGCC, 41 DGTS and 39 DGTA were identified from 14 species of microalgae in four major phyla. DGCC was the dominant betaine lipid in dinoflagellates and haptophytes, while DGTS was the main betaine lipid in chlorophytes. There were two main types of betaine lipid in diatoms: DGCC in centric diatom and DGTA in pennate diatom. The differences of fatty acid of betaine lipid in marine microalgae were only observed at the phyla and class levels, but at lower taxonomic levels the differences were less stable. DGCC existed in diatoms, dinoflagellates and chlorophytes, which contained C14−C18 saturated and unsaturated fatty acids and C20 and C22 polyunsaturated fatty acids. The difference in DGCC is that saturated C14−C18 fatty acids existed in dinoflagellates, while the fatty acids of odd carbon numbers C19 occurred only in diatoms. The results of description and molecular characterization of betaine lipid in microalgae can be served in future investigation in chemotaxonomy, physiology, ecological role of microalgae and functional properties of these phosphorous-lacking polar lipids.

, correspAuthors=Jilin Xu, authorNote=null, correspAuthorsNote=null, copyrightStatement=Haiyang Xuebao, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yanrong Li, Tong Mu, Lili Huang, Jilin Xu, Chengxu Zhou, Xiaojun Yan), CN=ArticleExt(id=1244313112380289400, articleId=1244313109855318302, tenantId=1146029695717560320, journalId=1149651085930835976, language=CN, title=甜菜碱脂在14种海洋微藻中的分布研究, columnId=1149698756456657529, journalTitle=海洋学报, columnName=论文, runingTitle=null, highlight=null, articleAbstract=

采用超高效液相色谱−四极杆−飞行时间质谱联用分析系统(UPLC -Q- TOF MS)，对4个微藻门中的14种微藻的甜菜碱脂的分子组成及其相应脂肪酸的组成及分布进行分析。结果表明：在14种微藻中共鉴定出133种甜菜碱脂，包括53种DGCC、41种DGTS和39种DGTA。其中甲藻和定鞭藻中主要的甜菜碱脂种类为DGCC，绿藻中主要的甜菜碱脂种类为DGTS；而在硅藻中的甜菜碱脂主要包括两种：中心硅藻纲中为DGCC，羽纹硅藻纲中为DGTA。此外，不同微藻中甜菜碱脂脂肪酸组成差异仅限制在门或纲的水平上，在较低的分类水平上差异不明显。硅藻门、甲藻门和定鞭藻门中均含有DGCC，其脂肪酸链的组成均含有C14−C18的脂肪酸以及C20和C22的多不饱和脂肪酸，但是甲藻的C14−C18脂肪酸链为饱和的，而C19奇数碳原子的脂肪酸链仅在硅藻中发现。认为海洋微藻甜菜碱脂的研究可以为海洋微藻化学分类学以及缺磷极性脂类的生理、生态作用和功能特性研究提供重要参考依据。

, correspAuthors=徐继林, authorNote=null, correspAuthorsNote=
*徐继林（1965-），男，研究员，主要从事微藻生物化学研究。E-mail：
, copyrightStatement=版权所有©《海洋学报》编辑部 2023
李艳荣，牟桐，黄莉莉，等. 甜菜碱脂在14种海洋微藻中的分布研究[J]. 海洋学报，2020，42(12)：72–81Li Yanrong，Mu Tong，Huang Lili, et al. Distribution of betaine lipids in 14 species of microalgae[J]. Haiyang Xuebao，2020, 42(12)：72–81
, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=oYl2ZqohFFJzMtNGfrYHTA==, magXml=DfTXchoIJvU+wrdhW2Qehg==, pdfUrl=null, pdf=dfOezr0H/jW8fgkvqWX1Yg==, pdfFileSize=915519, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=ncptEF/Fw4GklShR2Y2YIQ==, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=88H3exy0HqtUX4P4NPv2Pw==, mapNumber=null, authorCompany=null, fund=null, authors=

李艳荣（1989-），女，山东省郓城县人，博士研究生，从事微藻生物化学研究。E-mail：

, authorsList=李艳荣, 牟桐, 黄莉莉, 徐继林, 周成旭, 严小军)}, authors=[Author(id=1246521514397421626, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=lyr200810211@163.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1246521514498084933, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521514397421626, language=EN, stringName=Yanrong Li, firstName=Yanrong, middleName=null, lastName=Li, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1 Ningbo Institute of Oceanography, Ningbo 315832, China
2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1246521514607136842, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521514397421626, language=CN, stringName=李艳荣, firstName=艳荣, middleName=null, lastName=李, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1 宁波海洋研究院，浙江 宁波 315832
2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832, bio={"content":"

李艳荣（1989-），女，山东省郓城县人，博士研究生，从事微藻生物化学研究。E-mail：

"}, bioImg=null, bioContent=

李艳荣（1989-），女，山东省郓城县人，博士研究生，从事微藻生物化学研究。E-mail：

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1246521514204483626, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=1, ext=[AuthorCompanyExt(id=1246521514212872235, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Ningbo Institute of Oceanography, Ningbo 315832, China), AuthorCompanyExt(id=1246521514221260845, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 宁波海洋研究院，浙江 宁波 315832)]), AuthorCompany(id=1246521514288369713, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=2, ext=[AuthorCompanyExt(id=1246521514292564018, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China), AuthorCompanyExt(id=1246521514300952627, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832)])]), Author(id=1246521514707800147, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1246521514808463449, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521514707800147, language=EN, stringName=Tong Mu, firstName=Tong, middleName=null, lastName=Mu, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 Ningbo Institute of Oceanography, Ningbo 315832, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1246521514938486879, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521514707800147, language=CN, stringName=牟桐, firstName=桐, middleName=null, lastName=牟, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 宁波海洋研究院，浙江 宁波 315832, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1246521514204483626, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=1, ext=[AuthorCompanyExt(id=1246521514212872235, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Ningbo Institute of Oceanography, Ningbo 315832, China), AuthorCompanyExt(id=1246521514221260845, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 宁波海洋研究院，浙江 宁波 315832)])]), Author(id=1246521516565876850, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1246521516712677504, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521516565876850, language=EN, stringName=Lili Huang, firstName=Lili, middleName=null, lastName=Huang, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 Ningbo Institute of Oceanography, Ningbo 315832, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1246521516796563594, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521516565876850, language=CN, stringName=黄莉莉, firstName=莉莉, middleName=null, lastName=黄, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1 宁波海洋研究院，浙江 宁波 315832, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1246521514204483626, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=1, ext=[AuthorCompanyExt(id=1246521514212872235, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Ningbo Institute of Oceanography, Ningbo 315832, China), AuthorCompanyExt(id=1246521514221260845, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 宁波海洋研究院，浙江 宁波 315832)])]), Author(id=1246521516884643984, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, orderNo=3, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=xujilin@nbu.edu.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1246521516976918686, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521516884643984, language=EN, stringName=Jilin Xu, firstName=Jilin, middleName=null, lastName=Xu, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, *, address=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1246521517098553511, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521516884643984, language=CN, stringName=徐继林, firstName=继林, middleName=null, lastName=徐, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, *, address=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1246521514288369713, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=2, ext=[AuthorCompanyExt(id=1246521514292564018, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China), AuthorCompanyExt(id=1246521514300952627, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832)])]), Author(id=1246521517199216816, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, orderNo=4, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1246521517283102901, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521517199216816, language=EN, stringName=Chengxu Zhou, firstName=Chengxu, middleName=null, lastName=Zhou, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1246521517383766207, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521517199216816, language=CN, stringName=周成旭, firstName=成旭, middleName=null, lastName=周, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1246521514288369713, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=2, ext=[AuthorCompanyExt(id=1246521514292564018, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China), AuthorCompanyExt(id=1246521514300952627, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832)])]), Author(id=1246521517480235210, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, orderNo=5, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1246521517572509905, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521517480235210, language=EN, stringName=Xiaojun Yan, firstName=Xiaojun, middleName=null, lastName=Yan, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1246521517681561820, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, authorId=1246521517480235210, language=CN, stringName=严小军, firstName=小军, middleName=null, lastName=严, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=2, address=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1246521514288369713, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=2, ext=[AuthorCompanyExt(id=1246521514292564018, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China), AuthorCompanyExt(id=1246521514300952627, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832)])])], keywords=[Keyword(id=1246521517853528296, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, orderNo=1, keyword=microalgae), Keyword(id=1246521517933220079, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, orderNo=2, keyword=betaine lipids), Keyword(id=1246521518021300469, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, orderNo=3, keyword=UPLC-Q-TOF-MS), Keyword(id=1246521518130352382, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, orderNo=1, keyword=海洋微藻), Keyword(id=1246521518222627081, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, orderNo=2, keyword=甜菜碱脂), Keyword(id=1246521518285541652, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, orderNo=3, keyword=超高效液相色谱−四极杆−飞行时间质谱)], refs=[Reference(id=1246521521901031801, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=1, rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=Dembitsky V M. Betaine ether-linked glycerolipids: chemistry and biology[J]. Progress in Lipid Research, 1996, 35(1): 1−51., articleTitle=null, refAbstract=null), Reference(id=1246521522043638140, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=2, rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=Kumari P, Bijo A J, Mantri V A, et al. Fatty acid profiling of tropical marine macroalgae: an analysis from chemotaxonomic and nutritional perspectives[J]. Phytochemistry, 2013, 86: 44−56., articleTitle=null, refAbstract=null), Reference(id=1246521522161078660, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=3, rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=Armada I, Hachero-Cruzado I, Mazuelos N, et al. Differences in betaine lipids and fatty acids between Pseudoisochrysis paradoxa VLP and Diacronema vlkianum VLP isolates (Haptophyta)[J]. Phytochemistry, 2013, 95: 224−233., articleTitle=null, refAbstract=null), Reference(id=1246521522261741959, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=4, rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=Banskota A H, Stefanova R, Sperker S, et al. New diacylglyceryltrimethylhomoserines from the marine microalga Nannochloropsis granulata and their nitric oxide inhibitory activity[J]. Journal of Applied Phycology, 2013, 25(5): 1513−1521., articleTitle=null, refAbstract=null), Reference(id=1246521522341433739, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=5, rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=Laloknam S, Tanaka K, Buaboocha T, et al. Halotolerant cyanobacterium Aphanothece halophytica contains a Betaine transporter active at alkaline pH and high salinity[J]. Applied and Environmental Microbiology, 2006, 72(9): 6018−6026., articleTitle=null, refAbstract=null), Reference(id=1246521522412736912, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=6, rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=Nakanishi K, Deuchi K. Culture of a high-chlorophyll-producing and halotolerant Chlorella vulgaris[J]. Journal of Bioscience and Bioengineering, 2014, 117(5): 617−619., articleTitle=null, refAbstract=null), Reference(id=1246521522484040086, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=7, rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=Bigogno C, Khozin-Goldberg I, Boussiba S, et al. Lipid and fatty acid composition of the green oleaginous alga Parietochloris incisa, the richest plant source of arachidonic acid[J]. Phytochemistry, 2002, 60(5): 497−503., articleTitle=null, refAbstract=null), Reference(id=1246521522593091994, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=8, rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=Sanina N M, Goncharova S N, Kostetsky E Y. Fatty acid composition of individual polar lipid classes from marine macrophytes[J]. Phytochemistry, 2004, 65(6): 721−730., articleTitle=null, refAbstract=null), Reference(id=1246521522689560991, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=9, rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=Martin P, Van Mooy B A S, Heithoff A, et al. Phosphorus supply drives rapid turnover of membrane phospholipids in the diatom Thalassiosira pseudonana[J]. The ISME Journal, 2010, 5(6): 1057−1060., articleTitle=null, refAbstract=null), Reference(id=1246521522769252774, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=10, rfOrder=9, authorNames=null, journalName=null, refType=null, unstructuredReference=Van Mooy B A S, Fredricks H F, Pedler B E, et al. Phytoplankton in the ocean use non-phosphorus lipids in response to phosphorus scarcity[J]. Nature, 2009, 458(7234): 69−72., articleTitle=null, refAbstract=null), Reference(id=1246521522861527465, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=11, rfOrder=10, authorNames=null, journalName=null, refType=null, unstructuredReference=Vogel G, Eichenberger W. Betaine lipids in lower plants. Biosynthesis of DGTS and DGTA in Ochromonas danica (Chrysophyceae) and the possible role of DGTS in lipid metabolism[J]. Plant and Cell Physiology, 1992, 33(4): 427−436., articleTitle=null, refAbstract=null), Reference(id=1246521522987356591, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=12, rfOrder=11, authorNames=null, journalName=null, refType=null, unstructuredReference=Eichenberger W, Gfeller H, Grey P, et al. Gas chromatographic-mass spectrometric identification of betaine lipids in Chroomonas salina[J]. Phytochemistry, 1996, 42(4): 967−972., articleTitle=null, refAbstract=null), Reference(id=1246521523088019890, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=13, rfOrder=12, authorNames=null, journalName=null, refType=null, unstructuredReference=Abida H, Dolch L J, Meï C, et al. Membrane Glycerolipid remodeling triggered by nitrogen and phosphorus starvation in Phaeodactylum tricornutum[J]. Plant Physiology, 2015, 167(1): 118−136., articleTitle=null, refAbstract=null), Reference(id=1246521523171905973, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=14, rfOrder=13, authorNames=null, journalName=null, refType=null, unstructuredReference=Roche S A, Leblond J D. Betaine lipids in chlorarachniophytes[J]. Phycological Research, 2010, 58(4): 298−305., articleTitle=null, refAbstract=null), Reference(id=1246521523280957881, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=15, rfOrder=14, authorNames=null, journalName=null, refType=null, unstructuredReference=Cañavate J P, Armada I, Ríos J L, et al. Exploring occurrence and molecular diversity of betaine lipids across taxonomy of marine microalgae[J]. Phytochemistry, 2016, 124: 68−78., articleTitle=null, refAbstract=null), Reference(id=1246521523385815485, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=16, rfOrder=15, authorNames=null, journalName=null, refType=null, unstructuredReference=Kröger N, Poulsen N. Diatoms-from cell wall biogenesis to nanotechnology[J]. Annual Review of Genetics, 2008, 42: 83−107., articleTitle=null, refAbstract=null), Reference(id=1246521523461312960, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=17, rfOrder=16, authorNames=null, journalName=null, refType=null, unstructuredReference=Li Yanrong, Lou Yamin, Mu Tong, et al. Simultaneous structural identification of diacylglyceryl-N-trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl-N, N, N-trimethyl-β-alanine (DGTA) in microalgae using dual Li+/H+ adduct ion mode by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. Rapid Communications in Mass Spectrometry, 2017, 31(5): 457−468., articleTitle=null, refAbstract=null), Reference(id=1246521523532616132, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=18, rfOrder=17, authorNames=null, journalName=null, refType=null, unstructuredReference=Kato M, Sakai M, Adachi K, et al. Distribution of betaine lipids in marine algae[J]. Phytochemistry, 1996, 42(5): 1341−1345., articleTitle=null, refAbstract=null), Reference(id=1246521523603919304, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=19, rfOrder=18, authorNames=null, journalName=null, refType=null, unstructuredReference=Flaim G, Obertegger U, Anesi A, et al. Temperature-induced changes in lipid biomarkers and mycosporine-like amino acids in the psychrophilic dinoflagellate Peridinium aciculiferum[J]. Freshwater Biology, 2014, 59(5): 985−997., articleTitle=null, refAbstract=null), Reference(id=1246521523675222478, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=20, rfOrder=19, authorNames=null, journalName=null, refType=null, unstructuredReference=Anesi A, Guella G. A fast liquid chromatography-mass spectrometry methodology for membrane lipid profiling through hydrophilic interaction liquid chromatography[J]. Journal of Chromatography A, 2015, 1384: 44−52., articleTitle=null, refAbstract=null), Reference(id=1246521523805245904, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=21, rfOrder=20, authorNames=null, journalName=null, refType=null, unstructuredReference=Van Mooy B A S, Fredricks H F. Bacterial and eukaryotic intact polar lipids in the eastern subtropical South Pacific: water-column distribution, planktonic sources, and fatty acid composition[J]. Geochimica et Cosmochimica Acta, 2010, 74(22): 6499−6516., articleTitle=null, refAbstract=null), Reference(id=1246521523905909207, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=22, rfOrder=21, authorNames=null, journalName=null, refType=null, unstructuredReference=Haigh W G, Yoder T F, Ericson L, et al. The characterisation and cyclic production of a highly unsaturated homoserine lipid in Chlorella minutissima[J]. Biochimica et Biophysica Acta (BBA)—Lipids and Lipid Metabolism, 1996, 1299(2): 183−190., articleTitle=null, refAbstract=null), Reference(id=1246521525453607388, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=23, rfOrder=22, authorNames=null, journalName=null, refType=null, unstructuredReference=Galloway A W E, Britton-Simmons K H, Duggins D O, et al. Fatty acid signatures differentiate marine macrophytes at ordinal and family ranks[J]. Journal of Phycology, 2012, 48(4): 956−965., articleTitle=null, refAbstract=null), Reference(id=1246521525592019425, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=24, rfOrder=23, authorNames=null, journalName=null, refType=null, unstructuredReference=Dalsgaard J, John M S, Kattner G, et al. Fatty acid trophic markers in the pelagic marine environment[J]. Advances in Marine Biology, 2003, 46: 225−340., articleTitle=null, refAbstract=null), Reference(id=1246521525675905506, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=25, rfOrder=24, authorNames=null, journalName=null, refType=null, unstructuredReference=Thomas F, Ladislav H, Imke L, et al. Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection[J]. BMC Plant Biology, 2011, 11(1): 124., articleTitle=null, refAbstract=null), Reference(id=1246521525776568805, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=26, rfOrder=25, authorNames=null, journalName=null, refType=null, unstructuredReference=Popendorf K J, Tanaka T, Pujo-Pay M, et al. Gradients in intact polar diacylglycerolipids across the Mediterranean Sea are related to phosphate availability[J]. Biogeosciences, 2011, 8(12): 3733−3745., articleTitle=null, refAbstract=null), Reference(id=1246521525860454890, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=27, rfOrder=26, authorNames=null, journalName=null, refType=null, unstructuredReference=Brandsma J, Hopmans E C, Brussaard C P D, et al. Spatial distribution of intact polar lipids in North Sea surface waters: relationship with environmental conditions and microbial community composition[J]. Limnology and Oceanography, 2013, 57(4): 959−973., articleTitle=null, refAbstract=null)], funds=[Fund(id=1246521521674539376, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, awardId=null, language=CN, fundingSource=国家重点研发计划（2019YFD0900400）；浙江省科技重大专项（2019C02057）；宁波市“科技创新2025”重大专项（2019B10006）；国家现代农业产业技术体系建设专项资金项目(CARS-49)；宁波市十三五海洋经济创新发展示范项目（NBHY-2017-P2）。, fundOrder=null, country=null)], companyList=[AuthorCompany(id=1246521514204483626, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=1, ext=[AuthorCompanyExt(id=1246521514212872235, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 Ningbo Institute of Oceanography, Ningbo 315832, China), AuthorCompanyExt(id=1246521514221260845, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514204483626, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1 宁波海洋研究院，浙江 宁波 315832)]), AuthorCompany(id=1246521514288369713, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, xref=2, ext=[AuthorCompanyExt(id=1246521514292564018, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China), AuthorCompanyExt(id=1246521514300952627, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, companyId=1246521514288369713, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832)])], figs=[ArticleFig(id=1246521518486868255, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, label=Fig. 1, caption=EIC of 16:0/22:6 DGCC ([M+H]+, m/z= 800.61) in T. pseudonana at the low collision energy of 6 V in the positive mode with the MSE technique (a); the MS2 spectrum of the [M+H]+ ion at m/z=800.61 of 16:0/22:6 DGCC at retention time 12.98 min extracted from T. pseudonana (b), figureFileSmall=omsVDp+4247Lhelf48VY0Q==, figureFileBig=EG32765YkVtlfMQ6C0ixcg==, tableContent=null), ArticleFig(id=1246521518583337255, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, label=图1, caption=正离子MSE模式，假微型海链藻中16:0/22:6 DGCC ([M+H]+，m/z=800.61)低能量下提取离子流色谱图 （a）；正离子模式下假微型海链藻保留时间为12.98 min16:0/22:6 DGCC [M+H]+（m/z=800.61）二级质谱图（b）, figureFileSmall=omsVDp+4247Lhelf48VY0Q==, figureFileBig=EG32765YkVtlfMQ6C0ixcg==, tableContent=null), ArticleFig(id=1246521518700777775, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, label=Fig. 2, caption=TIC of the lipid mixture extracted from P. carterae at the low collision energy of 6 V in the positive mode with the MSE technique (a); EIC of m/z=104.11 for characteristic product ion of DGCC in P. carterae from high collision energy scans (ramp of 30–60 V) in the positive mode with the MSE technique (b), figureFileSmall=fC10SiM4Ll/kpwmUG62syg==, figureFileBig=pV1plFdsTuMjP2GCrkSrqg==, tableContent=null), ArticleFig(id=1246521518788858163, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, label=图2, caption=正离子MSE模式，颗石藻总脂混合物的低能量总离子流色谱图（a）；高能量MSE色谱图中提取DGCC特征碎片离子m/z=104.11的离子流色谱图（b）, figureFileSmall=fC10SiM4Ll/kpwmUG62syg==, figureFileBig=pV1plFdsTuMjP2GCrkSrqg==, tableContent=null), ArticleFig(id=1246521518910492988, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, label=Table 1, caption=

The strain number and species name of 14 species of microalgae

, figureFileSmall=null, figureFileBig=null, tableContent=
序号分类种质编号中文名称拉丁名称
1甲藻门NMBjah045东海原甲藻Prorocentrum donghaiense
2NMBjah047-1卡罗藻Karlodinum veneficum
3NMBjah043微小亚历山大藻Alexandrium minutum
4绿藻门NMBluh015-1小球藻Chlorella sp.
5NMBluh014微绿球藻Nannochloropsis oceanica
6NMBluh014-1云微藻Nannochloris sp.
7定鞭藻门NMBjih026-1颗石藻Pleurochrysis carterae
8NMBjih022-2湛江等鞭金藻Isochrysis zhanjiangensis
9NMBjih021-2球等鞭金藻Isochrysis galbana
10硅藻门NMBguh003-4角毛藻Chaetoceros sp.
11NMBguh005假微型海链藻Thalassiosira pseudonana
12NMBguh021威氏海链藻Conticribra weissflogii
13NMBguh001三角褐指藻Phaeodactylum tricornutum
14NMBguh002小新月菱形藻Nitzschia closterium f. minutissima
), ArticleFig(id=1246521519006961985, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, label=表1, caption=

14种海洋微藻的种名和种质编号

, figureFileSmall=null, figureFileBig=null, tableContent=
序号分类种质编号中文名称拉丁名称
1甲藻门NMBjah045东海原甲藻Prorocentrum donghaiense
2NMBjah047-1卡罗藻Karlodinum veneficum
3NMBjah043微小亚历山大藻Alexandrium minutum
4绿藻门NMBluh015-1小球藻Chlorella sp.
5NMBluh014微绿球藻Nannochloropsis oceanica
6NMBluh014-1云微藻Nannochloris sp.
7定鞭藻门NMBjih026-1颗石藻Pleurochrysis carterae
8NMBjih022-2湛江等鞭金藻Isochrysis zhanjiangensis
9NMBjih021-2球等鞭金藻Isochrysis galbana
10硅藻门NMBguh003-4角毛藻Chaetoceros sp.
11NMBguh005假微型海链藻Thalassiosira pseudonana
12NMBguh021威氏海链藻Conticribra weissflogii
13NMBguh001三角褐指藻Phaeodactylum tricornutum
14NMBguh002小新月菱形藻Nitzschia closterium f. minutissima
), ArticleFig(id=1246521519124402504, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, label=Table 2, caption=

The composition and content of DGCC in 9 microalgae（nmol/mg dry microalgae）

, figureFileSmall=null, figureFileBig=null, tableContent=
组成[M+H]+sn-1/sn-2硅藻门甲藻门定鞭藻门
假微型海链藻威氏海链藻角毛藻 东海原甲藻卡罗藻微小亚历山大藻 颗石藻湛江等鞭金藻球等鞭金藻
DGCC-1462.3414:00.026±0.0020.057±0.0080.017±0.0010.013±0.0030.007±0.0020.010±0.0020.059±0.010
DGCC-2486.3516:20.004±0.001
DGCC-3488.3616:10.017±0.0020.474±0.031
DGCC-4490.3716:00.055±0.0040.219±0.0270.139±0.0140.125±0.0310.075±0.0151.053±0.0800.025±0.003
DGCC-5504.3917:00.016±0.004
DGCC-6510.3418:40.077±0.005
DGCC-7514.3718:20.191±0.0190.059±0.003
DGCC-8516.3918:10.009±0.0010.153±0.0160.096±0.0060.133±0.011
DGCC-9518.418:00.122±0.006
DGCC-10536.3620:50.046±0.0040.628±0.0910.423±0.0280.418±0.034
DGCC-11538.3720:40.218±0.009
DGCC-12562.3722:60.053±0.0040.739±0.0860.272±0.0180.046±0.0081.419±0.0540.135±0.0360.691±0.065
DGCC-13672.5414:0/14:00.016±0.0020.020±0.0020.118±0.007
DGCC-14698.5614:0/16:10.144±0.0050.326±0.020
DGCC-15700.5314:0/16:00.027±0.0070.025±0.0030.281±0.0200.047±0.005
DGCC-16720.5316:0/16:40.178±0.009
DGCC-17720.5414:0/18:40.298±0.0490.112±0.0340.139±0.012
DGCC-18724.5716:1/16:10.091±0.0050.430±0.024
DGCC-19726.5916:0/16:10.175±0.0090.205±0.0200.421±0.0160.030±0.005
DGCC-20728.616:0/16:00.092±0.0310.012±0.003
DGCC-21746.5616:0/18:50.255±0.012
DGCC-22746.5514:0/20:50.404±0.0131.762±0.1700.010±0.003
DGCC-23748.5716:0/18:40.103±0.0042.990±0.059
DGCC-24752.5916:0/18:20.095±0.0070.244±0.034
DGCC-25754.6316:0/18:10.055±0.0020.164±0.022
DGCC-26756.6416:0/18:00.031±0.007
DGCC-27768.5416:3/20:50.072±0.005
DGCC-28770.5616:2/20:50.153±0.010
DGCC-29772.5816:1/20:50.728±0.0450.855±0.0111.002±0.065
DGCC-30772.5714:0/22:60.902±0.0600.574±0.0280.097±0.0181.542±0.2609.156±0.935
DGCC-31774.5914:0/22:50.017±0.002
DGCC-32774.5816:0/20:50.124±0.0050.993±0.1730.354±0.0180.023±0.0048.025±0.171
DGCC-33776.6116:0/20:40.131±0.0120.478±0.033
DGCC-34780.6318:1/18:10.056±0.003
DGCC-35794.5518:4/20:50.142±0.0090.224±0.005
DGCC-36794.5516:3/22:60.077±0.038
DGCC-37796.5818:3/20:50.355±0.063
DGCC-38798.616:1/22:60.179±0.0100.145±0.016
DGCC-39798.5716:0/22:50.073±0.014
), ArticleFig(id=1246521519216677197, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, label=表2, caption=

9种海洋微藻中DGCC的组成及含量（nmol/mg干藻粉）

, figureFileSmall=null, figureFileBig=null, tableContent=
组成[M+H]+sn-1/sn-2硅藻门甲藻门定鞭藻门
假微型海链藻威氏海链藻角毛藻 东海原甲藻卡罗藻微小亚历山大藻 颗石藻湛江等鞭金藻球等鞭金藻
DGCC-1462.3414:00.026±0.0020.057±0.0080.017±0.0010.013±0.0030.007±0.0020.010±0.0020.059±0.010
DGCC-2486.3516:20.004±0.001
DGCC-3488.3616:10.017±0.0020.474±0.031
DGCC-4490.3716:00.055±0.0040.219±0.0270.139±0.0140.125±0.0310.075±0.0151.053±0.0800.025±0.003
DGCC-5504.3917:00.016±0.004
DGCC-6510.3418:40.077±0.005
DGCC-7514.3718:20.191±0.0190.059±0.003
DGCC-8516.3918:10.009±0.0010.153±0.0160.096±0.0060.133±0.011
DGCC-9518.418:00.122±0.006
DGCC-10536.3620:50.046±0.0040.628±0.0910.423±0.0280.418±0.034
DGCC-11538.3720:40.218±0.009
DGCC-12562.3722:60.053±0.0040.739±0.0860.272±0.0180.046±0.0081.419±0.0540.135±0.0360.691±0.065
DGCC-13672.5414:0/14:00.016±0.0020.020±0.0020.118±0.007
DGCC-14698.5614:0/16:10.144±0.0050.326±0.020
DGCC-15700.5314:0/16:00.027±0.0070.025±0.0030.281±0.0200.047±0.005
DGCC-16720.5316:0/16:40.178±0.009
DGCC-17720.5414:0/18:40.298±0.0490.112±0.0340.139±0.012
DGCC-18724.5716:1/16:10.091±0.0050.430±0.024
DGCC-19726.5916:0/16:10.175±0.0090.205±0.0200.421±0.0160.030±0.005
DGCC-20728.616:0/16:00.092±0.0310.012±0.003
DGCC-21746.5616:0/18:50.255±0.012
DGCC-22746.5514:0/20:50.404±0.0131.762±0.1700.010±0.003
DGCC-23748.5716:0/18:40.103±0.0042.990±0.059
DGCC-24752.5916:0/18:20.095±0.0070.244±0.034
DGCC-25754.6316:0/18:10.055±0.0020.164±0.022
DGCC-26756.6416:0/18:00.031±0.007
DGCC-27768.5416:3/20:50.072±0.005
DGCC-28770.5616:2/20:50.153±0.010
DGCC-29772.5816:1/20:50.728±0.0450.855±0.0111.002±0.065
DGCC-30772.5714:0/22:60.902±0.0600.574±0.0280.097±0.0181.542±0.2609.156±0.935
DGCC-31774.5914:0/22:50.017±0.002
DGCC-32774.5816:0/20:50.124±0.0050.993±0.1730.354±0.0180.023±0.0048.025±0.171
DGCC-33776.6116:0/20:40.131±0.0120.478±0.033
DGCC-34780.6318:1/18:10.056±0.003
DGCC-35794.5518:4/20:50.142±0.0090.224±0.005
DGCC-36794.5516:3/22:60.077±0.038
DGCC-37796.5818:3/20:50.355±0.063
DGCC-38798.616:1/22:60.179±0.0100.145±0.016
DGCC-39798.5716:0/22:50.073±0.014
), ArticleFig(id=1246521519334117715, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, label=Table 4, caption=

The composition and content of betaine lipid in 3 microalgae（nmol/mg dry microalgae）

, figureFileSmall=null, figureFileBig=null, tableContent=
组成[M+H]+sn-1/sn-2硅藻门甲藻门
小新月菱形藻三角褐指藻 卡罗藻
DGTA-1472.3616:10.173±0.001
DGTA-2496.3618:30.598±0.016
DGTA-3498.3818:20.554±0.0500.523±0.033
DGTA-4500.3918:11.744±0.1290.686±0.040
DGTA-5520.3520:50.227±0.0171.115±0.102
DGTA-6522.3820:40.172±0.0081.593±0.095
DGTA-7546.3722:60.383±0.0340.336±0.019
DGTA-8654.5314:0/14:10.146±0.004
DGTA-9656.5414:0/14:00.579±0.035
DGTA-10682.5714:0/16:10.188±0.0280.556±0.033
DGTA-11704.5514:0/18:40.060±0.006
DGTA-12706.5714:0/18:30.408±0.023
DGTA-13708.5816:1/16:10.081±0.005
DGTA-14708.5714:0/18:20.066±0.0070.621±0.039
DGTA-15710.5914:0/18:11.024±0.1520.863±0.049
DGTA-16730.5714:0/20:50.268±0.0311.260±0.106
DGTA-17732.5714:0/20:40.072±0.0211.303±0.045
DGTA-18734.5914:0/20:30.301±0.033
DGTA-19734.5916:1/18:40.047±0.002
DGTA-20736.6216:1/18:10.361±0.011
DGTA-21736.616:0/18:20.193±0.012
DGTA-22738.6216:0/18:10.125±0.0150.126±0.008
DGTA-23752.5516:3/20:50.021±0.004
DGTA-24754.5616:2/20:50.026±0.0030.014±0.002
DGTA-25756.5716:1/20:50.094±0.0070.209±0.030
DGTA-26756.5714:0/22:60.025±0.0020.507±0.026
DGTA-27758.5916:1/20:40.350±0.016
DGTA-28758.616:0/20:50.187±0.014
DGTA-29760.6116:0/20:40.026±0.0030.209±0.015
DGTA-30764.6418:1/18:10.112±0.021
DGTA-31778.5618:4/20:50.035±0.003
DGTA-32780.5818:3/20:50.030±0.0010.431±0.032
DGTA-33782.5916:1/22:60.070±0.005
DGTA-34784.6118:1/20:50.517±0.0260.603±0.042
DGTA-35804.5720:5/20:50.776±0.1440.588±0.029
DGTA-36806.5920:4/20:50.078±0.0130.358±0.039
DGTA-37806.5920:5/20:41.189±0.098
DGTA-38808.620:4/20:40.226±0.012
DGTA-39830.5920:5/22:60.065±0.0030.247±0.015
), ArticleFig(id=1246521519434781012, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, label=表4, caption=

3种海洋微藻中甜菜碱脂的组成及含量（nmol/mg干藻粉）

, figureFileSmall=null, figureFileBig=null, tableContent=
组成[M+H]+sn-1/sn-2硅藻门甲藻门
小新月菱形藻三角褐指藻 卡罗藻
DGTA-1472.3616:10.173±0.001
DGTA-2496.3618:30.598±0.016
DGTA-3498.3818:20.554±0.0500.523±0.033
DGTA-4500.3918:11.744±0.1290.686±0.040
DGTA-5520.3520:50.227±0.0171.115±0.102
DGTA-6522.3820:40.172±0.0081.593±0.095
DGTA-7546.3722:60.383±0.0340.336±0.019
DGTA-8654.5314:0/14:10.146±0.004
DGTA-9656.5414:0/14:00.579±0.035
DGTA-10682.5714:0/16:10.188±0.0280.556±0.033
DGTA-11704.5514:0/18:40.060±0.006
DGTA-12706.5714:0/18:30.408±0.023
DGTA-13708.5816:1/16:10.081±0.005
DGTA-14708.5714:0/18:20.066±0.0070.621±0.039
DGTA-15710.5914:0/18:11.024±0.1520.863±0.049
DGTA-16730.5714:0/20:50.268±0.0311.260±0.106
DGTA-17732.5714:0/20:40.072±0.0211.303±0.045
DGTA-18734.5914:0/20:30.301±0.033
DGTA-19734.5916:1/18:40.047±0.002
DGTA-20736.6216:1/18:10.361±0.011
DGTA-21736.616:0/18:20.193±0.012
DGTA-22738.6216:0/18:10.125±0.0150.126±0.008
DGTA-23752.5516:3/20:50.021±0.004
DGTA-24754.5616:2/20:50.026±0.0030.014±0.002
DGTA-25756.5716:1/20:50.094±0.0070.209±0.030
DGTA-26756.5714:0/22:60.025±0.0020.507±0.026
DGTA-27758.5916:1/20:40.350±0.016
DGTA-28758.616:0/20:50.187±0.014
DGTA-29760.6116:0/20:40.026±0.0030.209±0.015
DGTA-30764.6418:1/18:10.112±0.021
DGTA-31778.5618:4/20:50.035±0.003
DGTA-32780.5818:3/20:50.030±0.0010.431±0.032
DGTA-33782.5916:1/22:60.070±0.005
DGTA-34784.6118:1/20:50.517±0.0260.603±0.042
DGTA-35804.5720:5/20:50.776±0.1440.588±0.029
DGTA-36806.5920:4/20:50.078±0.0130.358±0.039
DGTA-37806.5920:5/20:41.189±0.098
DGTA-38808.620:4/20:40.226±0.012
DGTA-39830.5920:5/22:60.065±0.0030.247±0.015
), ArticleFig(id=1246521520995062108, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, label=Table 3, caption=

The composition and content of DGTS in 8 microalgae（nmol/mg dry microalgae）

, figureFileSmall=null, figureFileBig=null, tableContent=
组成[M+H]+ sn-1/sn-2硅藻门甲藻门绿藻门定鞭藻门
威氏海链藻 卡罗藻 小球藻微绿球藻云微藻 颗石藻湛江等鞭金藻球等鞭金藻
DGTS-1472.3616:10.214±0.0450.332±0.060
DGTS-2474.3816:00.156±0.0270.175±0.0320.045±0.002
DGTS-3496.3618:30.191±0.0340.215±0.005
DGTS-4498.3818:20.039±0.0060.053±0.0100.063±0.014
DGTS-5520.3620:51.305±0.3222.247±0.303
DGTS-6522.3720:40.136±0.0280.487±0.099
DGTS-7682.5714:0/16:10.434±0.0820.105±0.007
DGTS-8706.5616:0/16:30.168±0.039
DGTS-9708.5714:0/18:20.080±0.008
DGTS-10708.5716:1/16:10.258±0.0460.099±0.004
DGTS-11708.5916:0/16:20.077±0.013
DGTS-12710.5914:0/18:10.568±0.0820.482±0.051
DGTS-13710.5716:0/16:10.138±0.0131.509±0.1830.748±0.031
DGTS-14724.5916:0/17:10.071±0.007
DGTS-15728.5516:3/18:30.035±0.006
DGTS-16730.5716:3/18:20.037±0.005
DGTS-17730.5514:0/20:50.546±0.0450.263±0.006
DGTS-18732.5714:0/20:40.131±0.023
DGTS-19732.5816:3/18:10.045±0.010
DGTS-20734.5816:0/18:30.045±0.0060.130±0.016
DGTS-21734.5918:0/16:30.286±0.049
DGTS-22736.6116:0/18:20.119±0.0230.480±0.064
DGTS-23736.618:0/16:20.091±0.013
DGTS-24738.6316:0/18:10.033±0.0040.069±0.0060.037±0.001
DGTS-25754.5516:2/20:50.108±0.017
DGTS-26756.5716:1/20:51.186±0.0760.537±0.007
DGTS-27758.5916:0/20:52.622±0.2781.683±0.050
DGTS-28758.618:2/18:30.066±0.015
DGTS-29760.6118:2/18:20.055±0.003
DGTS-30760.6116:0/20:40.477±0.0770.417±0.011
DGTS-31762.6220:0/16:30.402±0.055
DGTS-32762.6318:1/18:20.011±0.00
DGTS-33764.6518:1/18:10.017±0.0060.020±0.002
DGTS-34764.6420:0/16:20.152±0.026
DGTS-35782.5918:2/20:50.340±0.056
DGTS-36784.618:1/20:50.286±0.038
DGTS-37784.5916:0/22:60.352±0.017
DGTS-38786.5918:1/20:40.062±0.007
DGTS-39792.6820:0/18:20.041±0.003
DGTS-40804.5620:5/20:53.023±0.3040.855±0.057
DGTS-41806.5820:4/20:51.015±0.0830.565±0.046
), ArticleFig(id=1246521521112502624, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, label=表3, caption=

8种海洋微藻中DGTS的组成及含量（nmol/mg干藻粉）

, figureFileSmall=null, figureFileBig=null, tableContent=
组成[M+H]+ sn-1/sn-2硅藻门甲藻门绿藻门定鞭藻门
威氏海链藻 卡罗藻 小球藻微绿球藻云微藻 颗石藻湛江等鞭金藻球等鞭金藻
DGTS-1472.3616:10.214±0.0450.332±0.060
DGTS-2474.3816:00.156±0.0270.175±0.0320.045±0.002
DGTS-3496.3618:30.191±0.0340.215±0.005
DGTS-4498.3818:20.039±0.0060.053±0.0100.063±0.014
DGTS-5520.3620:51.305±0.3222.247±0.303
DGTS-6522.3720:40.136±0.0280.487±0.099
DGTS-7682.5714:0/16:10.434±0.0820.105±0.007
DGTS-8706.5616:0/16:30.168±0.039
DGTS-9708.5714:0/18:20.080±0.008
DGTS-10708.5716:1/16:10.258±0.0460.099±0.004
DGTS-11708.5916:0/16:20.077±0.013
DGTS-12710.5914:0/18:10.568±0.0820.482±0.051
DGTS-13710.5716:0/16:10.138±0.0131.509±0.1830.748±0.031
DGTS-14724.5916:0/17:10.071±0.007
DGTS-15728.5516:3/18:30.035±0.006
DGTS-16730.5716:3/18:20.037±0.005
DGTS-17730.5514:0/20:50.546±0.0450.263±0.006
DGTS-18732.5714:0/20:40.131±0.023
DGTS-19732.5816:3/18:10.045±0.010
DGTS-20734.5816:0/18:30.045±0.0060.130±0.016
DGTS-21734.5918:0/16:30.286±0.049
DGTS-22736.6116:0/18:20.119±0.0230.480±0.064
DGTS-23736.618:0/16:20.091±0.013
DGTS-24738.6316:0/18:10.033±0.0040.069±0.0060.037±0.001
DGTS-25754.5516:2/20:50.108±0.017
DGTS-26756.5716:1/20:51.186±0.0760.537±0.007
DGTS-27758.5916:0/20:52.622±0.2781.683±0.050
DGTS-28758.618:2/18:30.066±0.015
DGTS-29760.6118:2/18:20.055±0.003
DGTS-30760.6116:0/20:40.477±0.0770.417±0.011
DGTS-31762.6220:0/16:30.402±0.055
DGTS-32762.6318:1/18:20.011±0.00
DGTS-33764.6518:1/18:10.017±0.0060.020±0.002
DGTS-34764.6420:0/16:20.152±0.026
DGTS-35782.5918:2/20:50.340±0.056
DGTS-36784.618:1/20:50.286±0.038
DGTS-37784.5916:0/22:60.352±0.017
DGTS-38786.5918:1/20:40.062±0.007
DGTS-39792.6820:0/18:20.041±0.003
DGTS-40804.5620:5/20:53.023±0.3040.855±0.057
DGTS-41806.5820:4/20:51.015±0.0830.565±0.046
), ArticleFig(id=1246521521242526051, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=EN, label=Table 5, caption=

Characteristics of fatty acids of betaine lipids in marine microalgae

, figureFileSmall=null, figureFileBig=null, tableContent=
硅藻门甲藻门绿藻门定鞭藻门
DGTA14:0, 16:0, 16:1, 16:2, 16:3, 18:1, 18:2, 18:3, 18:4, 20:3, 20:4, 20:5, 22:614:0, 14:114:0, 16:1, 18:3, 18:4, 20:5, 22:6
DGTS14:0, 15:0, 16:0,16:1, 17:1, 18:1, 18:218:1, 18:214:0, 16:0, 16:1, 16:2, 16:3, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:4, 20:514:0, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 18:4, 20:5, 22:6
DGCC14:0, 16:0, 16:1, 16:2, 16:3, 16:4, 18:1, 18:2, 18:3, 18:4, 19:0, 20:4, 20:5, 22:614:0, 16:0, 17:0, 18:0, 20:5, 22:614:0, 16:0, 16:1, 18:1, 18:3, 18:4, 18:5, 20:4, 20:5, 22:5, 22:6
), ArticleFig(id=1246521521376743782, tenantId=1146029695717560320, journalId=1149651085930835976, articleId=1244313109855318302, language=CN, label=表5, caption=

4个门的微藻中甜菜碱脂脂肪酸的组成特点

, figureFileSmall=null, figureFileBig=null, tableContent=
硅藻门甲藻门绿藻门定鞭藻门
DGTA14:0, 16:0, 16:1, 16:2, 16:3, 18:1, 18:2, 18:3, 18:4, 20:3, 20:4, 20:5, 22:614:0, 14:114:0, 16:1, 18:3, 18:4, 20:5, 22:6
DGTS14:0, 15:0, 16:0,16:1, 17:1, 18:1, 18:218:1, 18:214:0, 16:0, 16:1, 16:2, 16:3, 17:1, 18:0, 18:1, 18:2, 18:3, 20:0, 20:4, 20:514:0, 16:0, 16:1, 18:0, 18:1, 18:2, 18:3, 18:4, 20:5, 22:6
DGCC14:0, 16:0, 16:1, 16:2, 16:3, 16:4, 18:1, 18:2, 18:3, 18:4, 19:0, 20:4, 20:5, 22:614:0, 16:0, 17:0, 18:0, 20:5, 22:614:0, 16:0, 16:1, 18:1, 18:3, 18:4, 18:5, 20:4, 20:5, 22:5, 22:6
)], attaches=null, journal=Journal(id=1146441459026210850, delFlag=0, nameCn=海洋学报, nameEn=Haiyang Xuebao, nameHistory1=null, nameHistory2=null, issn=0253-4193, eissn=null, cn=11-2055/P, coden=null, periodic=0, language=CN, oaType=否, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=uYi7hkkrve+l8pIcwqcaQQ==, journalPrice=null, startedYear=null, abbrevIsoEn=null, journalRemark=null, publicationField=null, createdTime=1751262543687, updatedTime=1761729782936, createdBy=18614031015, updatedBy=13701087609, firstLetterCn=H, firstLetterEn=H, subjectCode=Natural Sciences, subjectName=Natural Sciences, subjectCodeEn=Natural Sciences, subjectNameEn=null, picCn=uYi7hkkrve+l8pIcwqcaQQ==, picEn=C0WLQb7uW3ok8EkkVOAGuw==, jcr=null, cjcr=null, exts=[JournalExt(id=1190344242636624294, language=CN, name=海洋学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=http://www.hyxbocean.cn/, createdTime=1761729782971, updatedTime=1761729782971, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://www.hyxb.org.cn/aos/ch/author/login.aspx, submissionEditorUrl=http://www.hyxb.org.cn/aos/ch/login.aspx, submissionReviewUrl=http://www.hyxb.org.cn/aos/ch/auditor/login.aspx, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1190344242712121767, language=EN, name=Haiyang Xuebao, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=http://www.aosocean.com/, createdTime=1761729782989, updatedTime=1761729782989, createdBy=13701087609, updatedBy=13701087609, submissionGuidelinesUrl=, submissionAuthorUrl=http://www.hyxb.org.cn/aos/ch/author/login.aspx, submissionEditorUrl=http://www.hyxb.org.cn/aos/ch/login.aspx, submissionReviewUrl=http://www.hyxb.org.cn/aos/ch/auditor/login.aspx, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1149651085930835976, websiteList=[Website(id=1188165202219512001, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1149651085930835976, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/hyxb/CN, language=CN, createTime=1761210259251, createBy=18614031015, updateTime=1761210330879, updateBy=18614031015, name=海洋学报-中文, tplId=1146099689490845704, title=海洋学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1188166688563413602, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202219512001, code=articleTextType, value=kx, createTime=1761210613623, updateTime=1761210613623, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166688538247775, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202219512001, code=banner, value=null, createTime=1761210613617, updateTime=1761210613617, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166688529859166, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202219512001, code=logo, value=https://castjournals.cast.org.cn/joweb/hyxb/CN/file/pic?fileId=BDEio/cxHnid8OD4QxrAYQ==, createTime=1761210613615, updateTime=1761210613615, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166688555024993, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202219512001, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/hyxb/CN/file/pic, createTime=1761210613621, updateTime=1761210613621, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166688546636384, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202219512001, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1761210613619, updateTime=1761210613619, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166688575996515, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202219512001, code=themeColor, value=null, createTime=1761210613626, updateTime=1761210613626, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166688596968036, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202219512001, code=themeStyle, value=null, createTime=1761210613631, updateTime=1761210613631, creator=18614031015, updator=18614031015)]), Website(id=1188165202282426564, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1149651085930835976, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/hyxb/EN, language=EN, createTime=1761210259266, createBy=18614031015, updateTime=1761210377920, updateBy=18614031015, name=海洋学报-英文, tplId=1146101810881728533, title=Haiyang Xuebao, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1188166798101856873, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202282426564, code=articleTextType, value=kx, createTime=1761210639739, updateTime=1761210639739, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166798076691046, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202282426564, code=banner, value=null, createTime=1761210639733, updateTime=1761210639733, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166798068302437, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202282426564, code=logo, value=https://castjournals.cast.org.cn/joweb/hyxb/EN/file/pic?fileId=BDEio/cxHnid8OD4QxrAYQ==, createTime=1761210639731, updateTime=1761210639731, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166798093468264, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202282426564, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/hyxb/EN/file/pic, createTime=1761210639737, updateTime=1761210639737, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166798085079655, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202282426564, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1761210639735, updateTime=1761210639735, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166798106051178, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202282426564, code=themeColor, value=null, createTime=1761210639740, updateTime=1761210639740, creator=18614031015, updator=18614031015), WebsiteProps(id=1188166798110245483, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1188165202282426564, code=themeStyle, value=null, createTime=1761210639741, updateTime=1761210639741, creator=18614031015, updator=18614031015)])], journalTitle=海洋学报, weixinUrl=null, journalUrl=http://www.hyxbocean.cn/, iacademicId=null, status=1, seqNo=null, journalTitleEn=Haiyang Xuebao, journalPhotoCn=uYi7hkkrve+l8pIcwqcaQQ==, journalPhotoEn=C0WLQb7uW3ok8EkkVOAGuw==, journalFirstLetter=H, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false), detailUrlCn=https://castjournals.cast.org.cn/joweb/hyxb/CN/10.3969/j.issn.0253-4193.2020.12.008, detailUrlEn=https://castjournals.cast.org.cn/joweb/hyxb/EN/10.3969/j.issn.0253-4193.2020.12.008, pdfUrlCn=https://castjournals.cast.org.cn/joweb/hyxb/CN/PDF/10.3969/j.issn.0253-4193.2020.12.008, pdfUrlEn=https://castjournals.cast.org.cn/joweb/hyxb/EN/PDF/10.3969/j.issn.0253-4193.2020.12.008, aliStartDate=null, aliEndDate=null, collectionFlag=false, citedCount=null, citedUrl=null, reference=null)
收藏切换
甜菜碱脂在14种海洋微藻中的分布研究
收藏切换
PDF下载
李艳荣 1, 2 , 牟桐 1 , 黄莉莉 1 , 徐继林 2, * , 周成旭 2 , 严小军 2
海洋学报 | 论文 2020,42(12): 72-81
收起
收藏切换
海洋学报 | 论文 2020, 42(12): 72-81
甜菜碱脂在14种海洋微藻中的分布研究
全屏
李艳荣1, 2 , 牟桐1, 黄莉莉1, 徐继林2, * , 周成旭2, 严小军2
作者信息
  • 1 宁波海洋研究院，浙江 宁波 315832
  • 2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832

    • 李艳荣（1989-），女，山东省郓城县人，博士研究生，从事微藻生物化学研究。E-mail：

通讯作者:

*徐继林（1965-），男，研究员，主要从事微藻生物化学研究。E-mail：
Distribution of betaine lipids in 14 species of microalgae
Yanrong Li1, 2 , Tong Mu1, Lili Huang1, Jilin Xu2, * , Chengxu Zhou2, Xiaojun Yan2
Affiliations
  • 1 Ningbo Institute of Oceanography, Ningbo 315832, China
  • 2 Key Laboratory of Applied Marine Biotechnology, Ministry of Education, Ningbo University, Ningbo 315832, China
出版时间: 2020-12-25 doi: 10.3969/j.issn.0253-4193.2020.12.008
文章导航
收藏切换
摘要
收起

采用超高效液相色谱−四极杆−飞行时间质谱联用分析系统(UPLC -Q- TOF MS)，对4个微藻门中的14种微藻的甜菜碱脂的分子组成及其相应脂肪酸的组成及分布进行分析。结果表明：在14种微藻中共鉴定出133种甜菜碱脂，包括53种DGCC、41种DGTS和39种DGTA。其中甲藻和定鞭藻中主要的甜菜碱脂种类为DGCC，绿藻中主要的甜菜碱脂种类为DGTS；而在硅藻中的甜菜碱脂主要包括两种：中心硅藻纲中为DGCC，羽纹硅藻纲中为DGTA。此外，不同微藻中甜菜碱脂脂肪酸组成差异仅限制在门或纲的水平上，在较低的分类水平上差异不明显。硅藻门、甲藻门和定鞭藻门中均含有DGCC，其脂肪酸链的组成均含有C14−C18的脂肪酸以及C20和C22的多不饱和脂肪酸，但是甲藻的C14−C18脂肪酸链为饱和的，而C19奇数碳原子的脂肪酸链仅在硅藻中发现。认为海洋微藻甜菜碱脂的研究可以为海洋微藻化学分类学以及缺磷极性脂类的生理、生态作用和功能特性研究提供重要参考依据。

海洋微藻  /  甜菜碱脂  /  超高效液相色谱−四极杆−飞行时间质谱

The qualitative and quantitative analyses on betaine lipids of microalgae were conducted by means of the quadrupole flight time ultra-high performance liquid chromatography tandem mass spectrometry (UPLC-Q-TOF-MS) using electron spraying ionization. As a result, 133 betaine lipids including 53 DGCC, 41 DGTS and 39 DGTA were identified from 14 species of microalgae in four major phyla. DGCC was the dominant betaine lipid in dinoflagellates and haptophytes, while DGTS was the main betaine lipid in chlorophytes. There were two main types of betaine lipid in diatoms: DGCC in centric diatom and DGTA in pennate diatom. The differences of fatty acid of betaine lipid in marine microalgae were only observed at the phyla and class levels, but at lower taxonomic levels the differences were less stable. DGCC existed in diatoms, dinoflagellates and chlorophytes, which contained C14−C18 saturated and unsaturated fatty acids and C20 and C22 polyunsaturated fatty acids. The difference in DGCC is that saturated C14−C18 fatty acids existed in dinoflagellates, while the fatty acids of odd carbon numbers C19 occurred only in diatoms. The results of description and molecular characterization of betaine lipid in microalgae can be served in future investigation in chemotaxonomy, physiology, ecological role of microalgae and functional properties of these phosphorous-lacking polar lipids.

microalgae  /  betaine lipids  /  UPLC-Q-TOF-MS
李艳荣, 牟桐, 黄莉莉, 徐继林, 周成旭, 严小军. 甜菜碱脂在14种海洋微藻中的分布研究. 海洋学报, 2020 , 42 (12) : 72 -81 . DOI: 10.3969/j.issn.0253-4193.2020.12.008
Yanrong Li, Tong Mu, Lili Huang, Jilin Xu, Chengxu Zhou, Xiaojun Yan. Distribution of betaine lipids in 14 species of microalgae[J]. Haiyang Xuebao, 2020 , 42 (12) : 72 -81 . DOI: 10.3969/j.issn.0253-4193.2020.12.008
正文
收起
1 引言
收起
甜菜碱脂是一类不含磷的甘油脂类，由两分子的脂肪酸和一分子的极性头部组成，Dembitsky[1]对甜菜碱脂的生化结构和生物学功能做了详细的报道。海洋微藻中的甜菜碱脂主要包括3大类：二酰甘油-N-三甲基高丝氨酸（diacylgycerol-N-trimethylhomoserine，DGTS），二酰甘油-羟甲基-N,N,N-三甲基-β-丙氨酸（diacylglyceryl-hydroxymethy-N,N,N- trimethyl-β-alanine，DGTA）和二酰甘油-O-羧羟甲基胆碱（diacylglyceryl-carboxyhydroxymethylcholine，DGCC），是海洋微藻中的三大膜脂之一。甜菜碱脂的组成和代谢对于微藻的化学分类具有重要的意义，甜菜碱脂的脂肪酸组成和完整极性脂分子可以作为营养标记，可进一步确认初级生产者在食物网中的作用[2-3]。Banskota等[4]在微绿球藻(Nannochloropsis granulata)中分离出6种DGTS，即(2S)-1,2-bis-Oeicosapentaenoylglyceryl-3-O-4′-(N,N,N-trimethyl)-homoserine, (2S)-1-O-eicosapentaenoyl-2-O-arachidonoylglyceryl-3-O-4′-(N,N,N-trimethyl)-homoserine, (2S)-1-Oeicosapentaenoyl-2-O-myristoylglyceryl-3-O-4′-(N,N,N-trimethyl) -homoserine, (2S)-1-O-eicosapentaenoyl-2-O-palmitoylglyceryl-3-O-4′-(N,N,N-trimethyl)-homoserine, (2S)-1-O-eicosapentaenoyl-2-O-palmitoleoylglyceryl-3-O-4′-(N,N,N-trimethyl)-homoserine和(2S)-1-O-eicosapentaenoyl-2-O-linoleoylglyceryl-3-O-4′-(N,N,N-trimethyl)-homoserine。这些DGTS可以通过下调一氧化氮合成酶的表达来抑制RAW264.7巨噬细胞中NO的产生，首次表明DGTS或许具有抗炎活性。此外，甜菜碱脂与微藻的生长和耐盐性有关，DGTS的含量测定有助于了解微藻生长或耐盐的机制，进而有益于培养出更多使用性微藻[5-6]。
海洋微藻是海洋中最主要的光合生物，硅藻、绿藻、甲藻和定鞭藻是4种常见的微藻种类，其季节、种类的变化在海洋生态环境、海区生产力、全球碳循环等方面起着重要作用[7-8]。甜菜碱脂是一类极性脂，是生物膜的重要组成成分，在磷胁迫时，甜菜碱脂可以替代磷脂酰胆碱（PC）维持藻类细胞正常的生命活动[9-10]。对于甜菜碱脂的研究，大多数研究者是将甜菜碱脂经过复杂的衍生化或酸化后用液相色谱、气相色谱或气相色谱−质谱联用的方法测定甜菜碱脂的组成[11-13]。近年来，液相色谱和质谱联用系统为海洋微藻中甜菜碱脂的研究提供了一种新的方法[3, 14-15]。本文利用超高效液相色谱−四级杆−飞行时间质谱联用分析系统（UPLC-Q-TOF MS）建立了一种同时检测海洋微藻中3类甜菜碱脂的分析方法，对4个微藻门中的14种微藻的甜菜碱脂分子结构及其相应脂肪酸的组成及分布进行分析。为海洋微藻生态学研究提供理论依据，是进一步阐明脂质代谢必然要做的一项工作，有助于更好地了解甜菜碱脂在人类健康领域的作用；另一方面，对不同种类海洋微藻中甜菜碱脂分布的研究，也可以为微藻的系统发育及化学分类提供新的见解。
2 材料与方法
收起
2.1 实验材料
DGTS标品1,2-Dipalmitoyl-sn-glycero-3-O-4-(N,N,N-trimethyl) homoserine (16:0-16:0 DGTS) 以及DGTS的同位素标准品（1,2-di-palmitoyl-sn-glycero-3-O-4'-[N,N,N-trimethyl（D9）-homoserine）均购买自美国Avanti Polar Lipids公司；所有用于样品提取、液质分析的试剂均为色谱级；纯水来自于Milli-Q纯水仪；抗氧化剂2，6-二叔丁基对甲酚（BHT，>99.9%），购自美国Sigma公司。
2.2 样品的培养和收集
表1所示的14种微藻均由宁波大学浙江省海洋生物重点实验室微藻种质库提供。用于微藻培养的海水均经脱脂棉过滤、煮沸消毒，所有容器均高温灭菌。培养液采用f/2配方[16]，藻种在5 000 mL锥形瓶中（20±2）℃自然光培养，每天摇动数次，并用颗粒粒度计数分析仪（上海求精生化试剂仪器有限公司）测量藻类密度，14种微藻均在平台期收获，将收获的藻液在5 000 r/min的条件下高速离心10 min获取藻泥，冷冻干燥机（美国Labconco公司）干燥后备用。样品平行培养3份。
2.3 样品制备
分别称取各藻类细胞50 mg干粉，加入氯仿/甲醇（1∶1，V/V）混合溶液提取总脂，氮气浓缩仪（美国ATR公司）吹干，甲醇复溶后经孔径为0.22 μm的超滤膜（美国Mil-lipore公司）离心过滤，然后进行UPLC-Q-TOF-MS（美国Waters公司）分析。所有溶剂中均加入50 μg/mL BHT。
2.4 UPLC-Q-TOF分析
用于样品分析的液相色谱和质谱条件见参考文献[17]。
2.5 甜菜碱脂的半定量分析
甜菜碱脂的半定量：分别在每一种微藻样品中加入5 μg/mL的1,2-dipalmitoyl-sn-glycero-3-O-4’-[N,N,Ntrimethyl(D9)] homoserine DGTS同位素内标（由于目前市场上只有DGTS的同位素内标和标准品，微藻中所有甜菜碱脂的半定量分析参照相应DGTS内标的峰面积）对样品进行半定量分析。根据流动相比例的改变，获得相应甜菜碱脂的离子化效果，从低碰撞能量下的色谱图中提取样品中每一种甜菜碱脂的离子色谱图；根据不同流动相比例下的甜菜碱脂的离子化效果，获得相应峰面积积分值。根据峰面积与浓度的线性关系，利用同位素标准品的浓度和峰面积半定量样品中每毫克干藻粉中甜菜碱脂含量，取3个平行结果的均值。
3 结果与讨论
收起
3.1 甜菜碱脂质谱规律及鉴定方法研究
由于目前市场上购买不到DGCC的相关标准品，文中选用文献已报道过的富含DGCC的微藻进行DGCC结构质谱碎裂规律的分析。据文献[15]报道，假微型海链藻中可检测到分子组成为16:0/22:6 DGCC，其母离子质荷比为799.59。利用UPLC-Q-TOF MS对假微型海链藻中16:0/22:6 DGCC的分子离子峰进行一级和二级质谱扫描。ESI源正离子模式的一级质谱图上(图1a)，m/z=800.61代表的是16:0/22:6 DGCC的[M+H]+的母离子峰；在碰撞能量为45 eV时的二级质谱图中（图1b），m/z=104.11、m/z=132.10和m/z=178.11的碎片离子的元素组成分别为[C5H14NO]+、[C6H14NO2]+和[C7H16NO4]+，这与Kato等[18]的研究结果一致，其中m/z=104.11碎片离子的丰度较高，可以作为DGCC的特征碎片离子，用于DGCC的定性。此外，m/z=472.37（[M+H-R2COOH]+）和m/z=544.36（[M+H-R2COOH]+）的碎片离子峰，可用于DGCC脂肪酸链的分析。
基于已总结的DGCC的质谱碎裂规律，采用MSE技术对微藻样品中的DGCC进行分析，可以在一次进样全扫中通过高低能量转换得到样品中每一个物质的母离子和对应子离子精确质量数。在MSE的二级质谱图中，母离子和子离子可以同时存在，从高碰撞能量扫描的总离子流图中提取含有m/z=104.11的特征碎片离子的色谱图；然后在相同保留时间点，从低碰撞能量扫描的总离子流图中找到相应碎片离子的[M+H]+的母离子；最后针对每一个母离子进行二级质谱分析，进一步确定DGCC的结构。例如，在颗石藻的高碰撞能量扫描的总离子流图中提取m/z=104.11碎片离子的色谱峰（图2b），从低碰撞能量扫描的总离子流图的对应保留时间找到每一个DGCC的母离子（图2a），然后针对每一个母离子分别进行二级质谱分析，对每一个DGCC的分子组成进行鉴定。通过进一步的二级质谱分析，在颗石藻中一共检测到17种DGCC甜菜碱脂（表2）。
DGTS和DGTA是两种同分异构体，[M+H]+的二级质谱碎裂方式非常相似，均可以产生特征碎片离子m/z=236.15和m/z=144.10。为了更有效地鉴定和区分这两种甜菜碱脂的分子组成，本文采用[M+H]+和[M+Li]+两种加合形式相结合的方法。在流动相中加入0.01% LiCl和0.1% HCOOH，可以同时检测到DGTS和DGTA的[M+H]+和[M+Li]+的母离子峰。首先在[M+H]+的条件下通过特征碎片离子和脂肪酸链的碎片离子鉴定出DGTS或DGTA的分子组成，然后在[M+Li]+的二级质谱图中根据碎片离子区分DGTS和DGTA。关于DGTS和DGTA具体的二级碎裂规律及详细鉴定方法参照本实验室已发表文献[17]。
3.2 14种海洋微藻中甜菜碱脂的组成和分布
根据以上研究的甜菜碱脂的特征碎片离子及其分析鉴定方法，对4个门的14种微藻中的甜菜碱脂组成进行分析。共鉴定出133种甜菜碱脂，包括53种DGCC、41种DGTS和39种DGTA（表2至表4）。本研究基于MSE技术建立的系统分析海洋微藻中甜菜碱脂的检测方法灵敏度更高，检测到甜菜碱脂的种类更多。Cañavate等[15] 在假微型海链藻中共检测到8种DGCC，而本研究在假微型海链藻中共检测出26种DGCC分子，其中还包括6种只含有一条脂肪酸链的甜菜碱脂，该方法鉴定的种类和数量具有明显优势。
Armada等[3]对两种定鞭金藻Pseudoisochrysis paradoxa VLP（等鞭金藻纲，Isochrysidaceae）和Diacronema vlkianum VLP（巴夫藻纲，Pavlovaceae）中的甜菜碱脂的研究发现，等鞭金藻纲的P. paradoxa VLP可以同时检测到3种类型的甜菜碱脂，而巴夫藻纲的D. vlkianum VLP中仅检测到DGCC和DGTS两种类型的甜菜碱脂，且14:0/18:1 DGTS占D. vlkianum VLP中总DGTS的90.9%以上。本研究的3种定鞭金藻中，湛江等鞭金藻和球等鞭金藻属于等鞭金藻纲，而颗石藻属于颗石藻纲，在湛江等鞭金藻和球等鞭金藻中除了DGCC外均检测到14:0/18:1 DGTS，这与Armada等[3]的研究结果一致，而颗石藻种检测到的DGTS脂肪酸链组成为16:0/22:6。研究结果表明，等鞭金藻纲主要的甜菜碱脂为DGCC，且仅含有或主要含有14:0/18:1 DGTS，进一步证明14:0/18:1 DGTS及其含量占比可以作为等鞭金藻纲的特征甜菜碱脂，为等鞭金藻门等鞭金藻纲藻的系统分类提供一种新的依据。
假微型海链藻中发现26种DGCC，威氏海链藻中发现25种DGCC和1种DGTS，角毛藻中发现16种DGCC；在小新月菱形藻和三角褐指藻中没有发现DGCC，但分别检测到27种和30种DGTA。由结果可知，中心硅藻纲（centric ditom）的3种硅藻，即假微型海链藻、威氏海链藻和角毛藻中检测到的甜菜碱脂主要为DGCC，没有检测到DGTA，而在羽纹硅藻纲（pennate diatom）的两种硅藻小新月菱形藻和三角褐指藻中检测到甜菜碱脂仅有DGTA。根据Vogel和Eichenberger[11]的研究，DGTS是DGTA合成的前体，即DGTA是通过DGTS极性头部脱羧基和重新形成羧基，以及甘油分子脱酰化和再酰化形成的。因此，所有含有DGTA的微藻原则上都应含有少量或痕量的DGTS，DGTS的水平取决于它转换成DGTA的速率。而小新月菱形藻和三角褐指藻中仅检测到DGTA，可能是因为DGTS含量太低，没有达到相应分析的检测限。双眉藻（Amphora sp.）属于羽纹硅藻纲的硅藻，Li等[17]在双眉藻（Amphora sp.）中共检测到16种DGTA和9种DGTS，没有检测到DGCC，且DGTA的种类和含量多于DGTS。说明这种生化组成的不同有助于鉴别硅藻的两个不同纲，相关的研究也证明了这一结论[9-10, 15, 18]。因此，海洋微藻进化过程中两个不同纲的硅藻甜菜碱脂的合成机制仍需要大量的研究去探索。
绿藻门的3种微藻中检测到的甜菜碱脂仅为DGTS，其种类和含量远远高于其他门微藻中的DGTS。目前关于甲藻门中甜菜碱脂分布的研究较少，Cañavate等[15]在环沟藻中仅检测到1种DGCC（16:0/16:0），Flaim等[19]和Anesi等[20]在甲藻中仅检测到DGCC存在。本研究中，东海原甲藻和微小亚历山大藻分别发现3种和10种DGCC，而在卡罗藻中同时检测到3种甜菜碱脂的存在，即11种DGCC、2种DGTS和2种DGTA。根据已有报道，本研究的卡罗藻是目前唯一报道过的同时含有3种甜菜碱脂的甲藻。
Cañavate等[15]利用主坐标分析（PCO）方法对微藻中的甜菜碱脂进行研究，结果表明绿藻纲、共球藻纲和大眼藻纲的微藻聚在第一坐标轴，与DGTS含量成正相关；DGTA和DGCC在第二坐标轴上分开，隐藻门和硅藻门的甜菜碱脂主要是DGTA，间藻门和甲藻门唯一的甜菜碱脂为DGCC。包括本研究在内的甜菜碱脂的研究都仅是一个初步的研究，还不能全面了解甜菜碱脂在微藻中的分布，但可以将甜菜碱脂作为完整极性脂的生物标记物，用于评估浮游植物天然的种群结构和生理状态[21]。
3.3 微藻中甜菜碱脂的脂肪酸组成
为了更好地了解不同门的微藻中甜菜碱脂分子的脂肪酸组成特点，将本研究中甜菜碱脂的脂肪酸组成检测结果与部分文献资料[3, 15, 18, 22]报道过的微藻中甜菜碱脂的脂肪酸组成相结合，对甲藻门、绿藻门、定鞭藻门和硅藻门四大类微藻中甜菜碱脂的脂肪酸组成进行了总结（表5）。由结果可知，硅藻门的甜菜碱脂DGTS中脂肪酸组成主要是C14−C18偶数个碳原子的饱和以及低不饱和短链脂肪酸，而DGTA和DGCC中的脂肪酸组成除了短链脂肪酸外，还含有多不饱和的脂肪酸和长链的多不饱和脂肪酸，如C20:4、C20:5和C22:6，该结果与Li等[17]对双眉藻Amphora sp.的脂肪酸组成研究结果一致。定鞭藻门3种类型甜菜碱脂的脂肪酸组成差别不大，基本为C14脂肪酸、C16脂肪酸、C18脂肪酸以及C20:5脂肪酸和C22:6脂肪酸；甲藻门中DGCC脂肪酸组成为C14饱和短链脂肪酸、C16饱和短链脂肪酸、C17饱和短链脂肪酸和C18饱和短链脂肪酸以及C20:5多不饱和脂肪酸和C22:6多不饱和脂肪酸，而DGTA和DGTS中仅含有短链脂肪酸。
此外，对不同门微藻中同一种类型甜菜碱脂的脂肪酸分布进行了分析，结果表明，硅藻门、甲藻门和定鞭藻门中均含有DGCC，但是甲藻门C14−C18脂肪酸链均为饱和的，而硅藻门中含有C19奇数碳原子的脂肪酸链。对于DGTS，硅藻中主要含有C14−C18的脂肪酸，甲藻中只含有C18的脂肪酸，C20的脂肪酸仅在绿藻门中检测到，而C22:6脂肪酸仅在定鞭藻中发现，这些特殊的脂肪酸组成的甜菜碱脂可在水产食物网研究中用作营养标记。
以上研究结果表明，不同门的微藻甜菜碱脂的脂肪酸组成有其各自的特点，同一个门内的不同微藻间甜菜碱脂脂肪酸的组成差别不大。相关研究表明，海洋大型植物中脂肪酸分布在科的水平上就可以很好地区分[23]，而微藻中甜菜碱脂的脂肪酸分布的特点与海洋大型植物中脂肪酸分布存在不同。Dalsgaard等[24]研究表明，微藻的脂肪酸组成在较高的分类水平上表现出一定的特点，在水产食物网研究中被广泛用作营养标记；Thomas等[25]研究也表明不同微藻脂肪酸组成差异只能限制在门和纲的水平上，在较低的分类水平上差异不明显。甜菜碱脂作为一种完整的极性脂已用于浮游植物自然群落结构的研究[26-27]，对甜菜碱脂脂肪酸组成的研究有望提高不同物种区分的分辨度，可以为微藻的系统发育及化学分类提供新的见解。
4 结论
收起
本研究运用超高效液相色谱−四级杆−飞行时间质谱建立了海洋微藻中甜菜碱脂的分析鉴定方法，对4个微藻门中的14种微藻的甜菜碱脂分子结构及其相应脂肪酸的组成及分布进行了研究。研究结果表明，这种简单快速的方法可以同时检测出微藻中3种类型的甜菜碱脂。不同门的微藻中甜菜碱脂分布的种类不同，定鞭藻和甲藻中主要的甜菜碱脂是DGCC，绿藻中主要的甜菜碱脂为DGTS，而在硅藻中的甜菜碱脂主要包括两种，即DGCC（中心硅藻纲）和DGTA（羽纹硅藻纲）。不同门的微藻甜菜碱脂的脂肪酸组成有其各自的特点，根据脂肪酸组成特点对微藻分类只能限制在门和纲的水平上，在较低的分类水平上差异不明显。本研究对微藻中甜菜碱脂的种类和分布特性进行了研究，对于进一步研究这些缺磷极性脂类的生理、生态作用和功能特性具有重要的意义，也可以为微藻的系统发育及化学分类提供新的见解。
基金
收起
  • 国家重点研发计划（2019YFD0900400）；浙江省科技重大专项（2019C02057）；宁波市“科技创新2025”重大专项（2019B10006）；国家现代农业产业技术体系建设专项资金项目(CARS-49)；宁波市十三五海洋经济创新发展示范项目（NBHY-2017-P2）。
文献
收起
参考文献 引证文献
排序方式：
1
Dembitsky V M. Betaine ether-linked glycerolipids: chemistry and biology[J]. Progress in Lipid Research, 1996, 35(1): 1−51.
2
Kumari P, Bijo A J, Mantri V A, et al. Fatty acid profiling of tropical marine macroalgae: an analysis from chemotaxonomic and nutritional perspectives[J]. Phytochemistry, 2013, 86: 44−56.
3
Armada I, Hachero-Cruzado I, Mazuelos N, et al. Differences in betaine lipids and fatty acids between Pseudoisochrysis paradoxa VLP and Diacronema vlkianum VLP isolates (Haptophyta)[J]. Phytochemistry, 2013, 95: 224−233.
4
Banskota A H, Stefanova R, Sperker S, et al. New diacylglyceryltrimethylhomoserines from the marine microalga Nannochloropsis granulata and their nitric oxide inhibitory activity[J]. Journal of Applied Phycology, 2013, 25(5): 1513−1521.
5
Laloknam S, Tanaka K, Buaboocha T, et al. Halotolerant cyanobacterium Aphanothece halophytica contains a Betaine transporter active at alkaline pH and high salinity[J]. Applied and Environmental Microbiology, 2006, 72(9): 6018−6026.
6
Nakanishi K, Deuchi K. Culture of a high-chlorophyll-producing and halotolerant Chlorella vulgaris[J]. Journal of Bioscience and Bioengineering, 2014, 117(5): 617−619.
7
Bigogno C, Khozin-Goldberg I, Boussiba S, et al. Lipid and fatty acid composition of the green oleaginous alga Parietochloris incisa, the richest plant source of arachidonic acid[J]. Phytochemistry, 2002, 60(5): 497−503.
8
Sanina N M, Goncharova S N, Kostetsky E Y. Fatty acid composition of individual polar lipid classes from marine macrophytes[J]. Phytochemistry, 2004, 65(6): 721−730.
9
Martin P, Van Mooy B A S, Heithoff A, et al. Phosphorus supply drives rapid turnover of membrane phospholipids in the diatom Thalassiosira pseudonana[J]. The ISME Journal, 2010, 5(6): 1057−1060.
10
Van Mooy B A S, Fredricks H F, Pedler B E, et al. Phytoplankton in the ocean use non-phosphorus lipids in response to phosphorus scarcity[J]. Nature, 2009, 458(7234): 69−72.
11
Vogel G, Eichenberger W. Betaine lipids in lower plants. Biosynthesis of DGTS and DGTA in Ochromonas danica (Chrysophyceae) and the possible role of DGTS in lipid metabolism[J]. Plant and Cell Physiology, 1992, 33(4): 427−436.
12
Eichenberger W, Gfeller H, Grey P, et al. Gas chromatographic-mass spectrometric identification of betaine lipids in Chroomonas salina[J]. Phytochemistry, 1996, 42(4): 967−972.
13
Abida H, Dolch L J, Meï C, et al. Membrane Glycerolipid remodeling triggered by nitrogen and phosphorus starvation in Phaeodactylum tricornutum[J]. Plant Physiology, 2015, 167(1): 118−136.
14
Roche S A, Leblond J D. Betaine lipids in chlorarachniophytes[J]. Phycological Research, 2010, 58(4): 298−305.
15
Cañavate J P, Armada I, Ríos J L, et al. Exploring occurrence and molecular diversity of betaine lipids across taxonomy of marine microalgae[J]. Phytochemistry, 2016, 124: 68−78.
16
Kröger N, Poulsen N. Diatoms-from cell wall biogenesis to nanotechnology[J]. Annual Review of Genetics, 2008, 42: 83−107.
17
Li Yanrong, Lou Yamin, Mu Tong, et al. Simultaneous structural identification of diacylglyceryl-N-trimethylhomoserine (DGTS) and diacylglycerylhydroxymethyl-N, N, N-trimethyl-β-alanine (DGTA) in microalgae using dual Li+/H+ adduct ion mode by ultra-performance liquid chromatography/quadrupole time-of-flight mass spectrometry[J]. Rapid Communications in Mass Spectrometry, 2017, 31(5): 457−468.
18
Kato M, Sakai M, Adachi K, et al. Distribution of betaine lipids in marine algae[J]. Phytochemistry, 1996, 42(5): 1341−1345.
19
Flaim G, Obertegger U, Anesi A, et al. Temperature-induced changes in lipid biomarkers and mycosporine-like amino acids in the psychrophilic dinoflagellate Peridinium aciculiferum[J]. Freshwater Biology, 2014, 59(5): 985−997.
20
Anesi A, Guella G. A fast liquid chromatography-mass spectrometry methodology for membrane lipid profiling through hydrophilic interaction liquid chromatography[J]. Journal of Chromatography A, 2015, 1384: 44−52.
21
Van Mooy B A S, Fredricks H F. Bacterial and eukaryotic intact polar lipids in the eastern subtropical South Pacific: water-column distribution, planktonic sources, and fatty acid composition[J]. Geochimica et Cosmochimica Acta, 2010, 74(22): 6499−6516.
22
Haigh W G, Yoder T F, Ericson L, et al. The characterisation and cyclic production of a highly unsaturated homoserine lipid in Chlorella minutissima[J]. Biochimica et Biophysica Acta (BBA)—Lipids and Lipid Metabolism, 1996, 1299(2): 183−190.
23
Galloway A W E, Britton-Simmons K H, Duggins D O, et al. Fatty acid signatures differentiate marine macrophytes at ordinal and family ranks[J]. Journal of Phycology, 2012, 48(4): 956−965.
24
Dalsgaard J, John M S, Kattner G, et al. Fatty acid trophic markers in the pelagic marine environment[J]. Advances in Marine Biology, 2003, 46: 225−340.
25
Thomas F, Ladislav H, Imke L, et al. Fatty acid profiles and their distribution patterns in microalgae: a comprehensive analysis of more than 2000 strains from the SAG culture collection[J]. BMC Plant Biology, 2011, 11(1): 124.
26
Popendorf K J, Tanaka T, Pujo-Pay M, et al. Gradients in intact polar diacylglycerolipids across the Mediterranean Sea are related to phosphate availability[J]. Biogeosciences, 2011, 8(12): 3733−3745.
27
Brandsma J, Hopmans E C, Brussaard C P D, et al. Spatial distribution of intact polar lipids in North Sea surface waters: relationship with environmental conditions and microbial community composition[J]. Limnology and Oceanography, 2013, 57(4): 959−973.
2020年第42卷第12期
PDF下载
88
38
引用本文
BibTeX
文章信息
doi: 10.3969/j.issn.0253-4193.2020.12.008
  • 接收时间：2019-09-23
  • 首发时间：2026-03-27
  • 出版时间：2020-12-25
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期：2019-09-23
  • 修回日期：2020-01-08
基金
国家重点研发计划（2019YFD0900400）；浙江省科技重大专项（2019C02057）；宁波市“科技创新2025”重大专项（2019B10006）；国家现代农业产业技术体系建设专项资金项目(CARS-49)；宁波市十三五海洋经济创新发展示范项目（NBHY-2017-P2）。
作者信息
    1 宁波海洋研究院，浙江 宁波 315832
    2 宁波大学 应用海洋生物技术教育部重点实验室，浙江 宁波 315832

通讯作者:

*徐继林（1965-），男，研究员，主要从事微藻生物化学研究。E-mail：
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/hyxb/CN/10.3969/j.issn.0253-4193.2020.12.008
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏
相关链接
论文
最新文章
热读文章
热点专题
内参
蓝皮书
产业发展报告
传播力报告
期刊分级目录
资讯
学术新闻
行业新闻
学术会议
行业会议
关于
关于我们
联系我们
北京市海淀区学院南路86号
100081
010-62199257
qkjq@cast.org.cn

中国科学技术协会版权所有 京ICP 备10216604号-4 海淀分局备案 1101084647
科技导报社主办 中国科协信息中心技术支持