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Article(id=1325115056077284121, tenantId=1146029695717560320, journalId=1255847867265597444, issueId=1325114700899426483, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1289923200000, receivedDateStr=2010-11-17, revisedDate=1297872000000, revisedDateStr=2011-02-17, acceptedDate=null, acceptedDateStr=null, onlineDate=1793861648815, onlineDateStr=2026-11-05, pubDate=1334419200000, pubDateStr=2012-04-15, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793861648815, onlineIssueDateStr=2026-11-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793861648815, creator=13701087609, updateTime=1793861648815, updator=13701087609, issue=Issue{id=1325114700899426483, tenantId=1146029695717560320, journalId=1255847867265597444, year='2012', volume='26', issue='2', pageStart='121', pageEnd='240', issueExtLink='null', onlineDate='null', pubDate='1334419200000', pubDateStr='2012-04-15', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1793861564134, creator='13701087609', updateTime=1793861564134, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=235, endPage=240, ext={EN=ArticleExt(id=1325115059512419101, articleId=1325115056077284121, tenantId=1146029695717560320, journalId=1255847867265597444, language=EN, title=Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii, columnId=null, journalTitle=Chinese Journal of High Pressure Physics, columnName=null, runingTitle=null, highlight=null, articleAbstract=

The activity of polyphenol oxidase (PPO) produced from Trametes Trogii was determined through using tea polyphenols as substrate. The pH value and the substrate concentration of strongest activity of PPO are 5.0 and 4 mg/mL, respectively. The activity of PPO processed by ultrahigh pressure of 100, 300 and 500 MPa for 10 min accounted for 93%, 87% and 86% of that of untreated one. The CD spectra of PPO processed by 100 and 300 MPa pressure respectively displayed folding shape and tillering shape of Cotton effect peak located in 190-210 nm, while the retention time of the first peak fraction (i.e., the maximum molecular weight fraction) of the two processed PPO on HPGPC profiles were advanced to 1.514 and 2.296 min, respectively from 6.678 min. Compared with control, CD spectrum and HPGPC of PPO treaded by 500 MPa showed more significant difference with only one positive Cotton effect peaks on CD spectra and reduced peak area of main components on HPGPC profiles (ie, the response value of 280 nm decreased). The disparity of CD and HPGPC of PPO treated by various UHPP showed the corresponding difference in the degree

, authors=Xiao-Qiang CHEN1, 2, Yin-Jun ZHANG3, Shi-Kang ZHANG1, Jian-Rong LI2, 6, Jian-Chang JIN4, Na ZHENG5, Xiu-Fang YANG1, authorsList=Xiao-Qiang CHEN, Yin-Jun ZHANG, Shi-Kang ZHANG, Jian-Rong LI, Jian-Chang JIN, Na ZHENG, Xiu-Fang YANG, authorCompany=null, correspAuthors=Shi-Kang ZHANG, Jian-Rong LI, Jian-Chang JIN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1325115063039828767, articleId=1325115056077284121, tenantId=1146029695717560320, journalId=1255847867265597444, language=CN, title=超高压处理对毛栓菌多酚氧化酶的影响, columnId=0, journalTitle=高压物理学报, columnName=, runingTitle=null, highlight=null, articleAbstract=

以茶多酚为底物，测定毛栓菌多酚氧化酶（Polyphenol Oxidase，PPO）活性的最适pH值为5.0、最适底物浓度为4 mg/mL，经100、300和500 MPa超高压处理10 min的PPO酶活分别是未处理的93%、87%和86%。圆二色谱和高效液相凝胶渗透色谱分析结果显示，100和300 MPa压力处理PPO的圆二色谱位于190~210 nm的正Cotton效应峰分别产生“褶皱”和“分蘖”；高效液相凝胶渗透色谱中的第一时间出峰组分（即最大分子量组分）的保留时间由6.678 min分别提前至1.514和2.296 min。500 MPa压力处理PPO的圆二色谱和高效液相凝胶渗透色谱与对照相比差异更加显著，其圆二色谱中只有一个正Cotton效应峰；其高效液相凝胶渗透色谱中主要组分的色谱峰面积减小（即280 nm的响应值降低）。不同压力处理的PPO的圆二色谱与高效液相凝胶渗透色谱图的差异程度具有对应关系。

, authors=陈小强1, 2, 章银军3, 张士康1, 励建荣2, 6, 金建昌4, 郑娜5, 杨秀芳1, authorsList=陈小强, 章银军, 张士康, 励建荣, 金建昌, 郑娜, 杨秀芳, authorCompany=null, correspAuthors=张士康, 励建荣, 金建昌, authorNote=

陈小强（1978—），男，副研究员，主要从事天然产物研究. E-mail：

, correspAuthorsNote=
张士康（1965—），男，博士，高级工程师，主要从事农副产品加工研究. E-mail：
励建荣（1964—），男，博士，教授，主要从事生鲜食品贮藏加工研究. E-Mail：
金建昌（1978—），男，博士，讲师，主要从事天然产物研究. E-mail：
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陈小强（1978—），男，副研究员，主要从事天然产物研究. E-mail：

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陈小强（1978—），男，副研究员，主要从事天然产物研究. E-mail：

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超高压处理对毛栓菌多酚氧化酶的影响
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陈小强 1, 2 , 章银军 3 , 张士康 1 , 励建荣 2, 6 , 金建昌 4 , 郑娜 5 , 杨秀芳 1
高压物理学报 | 2012,26(2): 235-240
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高压物理学报 | 2012 , 26 (2) : 235 -240
超高压处理对毛栓菌多酚氧化酶的影响
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陈小强1, 2 , 章银军3, 张士康1 , 励建荣2, 6 , 金建昌4 , 郑娜5, 杨秀芳1
作者信息
  • 1.中华全国供销合作总社杭州茶叶研究院，浙江 杭州　310016
  • 2.浙江工商大学食品与生物工程学院，浙江 杭州　310035
  • 3.浙江工业大学生物与环境工程学院，浙江 杭州　310014
  • 4.浙江树人大学生物与环境工程学院，浙江 杭州　310015
  • 5.浙江大学化学工程联合国家重点实验室，浙江 杭州　310027
  • 6.渤海大学，辽宁 锦州　121013
通讯作者:
张士康（1965—），男，博士，高级工程师，主要从事农副产品加工研究. E-mail：
励建荣（1964—），男，博士，教授，主要从事生鲜食品贮藏加工研究. E-Mail：
金建昌（1978—），男，博士，讲师，主要从事天然产物研究. E-mail：
作者简介:

陈小强（1978—），男，副研究员，主要从事天然产物研究. E-mail：

Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii
Xiao-Qiang CHEN1, 2 , Yin-Jun ZHANG3, Shi-Kang ZHANG1 , Jian-Rong LI2, 6 , Jian-Chang JIN4 , Na ZHENG5, Xiu-Fang YANG1
Affiliations
  • 1.Hangzhou Tea Research Institute, China COOP, Hangzhou 310016, China
  • 2.College of Food Science and Biotechnology, Zhejiang Gongshang University, Hangzhou 310035, China
  • 3.College of Biological and Envrionmental Engineering, Zhejiang University of Technology, Hangzhou 310014, China
  • 4.College of Biology and Environmental Engineering, Zhejiang Shuren University, Hangzhou 310015, China
  • 5.State Key Laboratory of Chemical Engineering, Zhejiang University, Hangzhou 310027, China
  • 6.Bohai University, Jinzhou 121013, China
出版时间: 2012-04-15
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摘要
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以茶多酚为底物，测定毛栓菌多酚氧化酶（Polyphenol Oxidase，PPO）活性的最适pH值为5.0、最适底物浓度为4 mg/mL，经100、300和500 MPa超高压处理10 min的PPO酶活分别是未处理的93%、87%和86%。圆二色谱和高效液相凝胶渗透色谱分析结果显示，100和300 MPa压力处理PPO的圆二色谱位于190~210 nm的正Cotton效应峰分别产生“褶皱”和“分蘖”；高效液相凝胶渗透色谱中的第一时间出峰组分（即最大分子量组分）的保留时间由6.678 min分别提前至1.514和2.296 min。500 MPa压力处理PPO的圆二色谱和高效液相凝胶渗透色谱与对照相比差异更加显著，其圆二色谱中只有一个正Cotton效应峰；其高效液相凝胶渗透色谱中主要组分的色谱峰面积减小（即280 nm的响应值降低）。不同压力处理的PPO的圆二色谱与高效液相凝胶渗透色谱图的差异程度具有对应关系。

超高压处理  /  多酚氧化酶  /  圆二色光谱  /  高效液相凝胶渗透色谱  /  毛栓菌

The activity of polyphenol oxidase (PPO) produced from Trametes Trogii was determined through using tea polyphenols as substrate. The pH value and the substrate concentration of strongest activity of PPO are 5.0 and 4 mg/mL, respectively. The activity of PPO processed by ultrahigh pressure of 100, 300 and 500 MPa for 10 min accounted for 93%, 87% and 86% of that of untreated one. The CD spectra of PPO processed by 100 and 300 MPa pressure respectively displayed folding shape and tillering shape of Cotton effect peak located in 190-210 nm, while the retention time of the first peak fraction (i.e., the maximum molecular weight fraction) of the two processed PPO on HPGPC profiles were advanced to 1.514 and 2.296 min, respectively from 6.678 min. Compared with control, CD spectrum and HPGPC of PPO treaded by 500 MPa showed more significant difference with only one positive Cotton effect peaks on CD spectra and reduced peak area of main components on HPGPC profiles (ie, the response value of 280 nm decreased). The disparity of CD and HPGPC of PPO treated by various UHPP showed the corresponding difference in the degree

ultrahigh pressure processing (UHPP)  /  polyphenol oxidase (PPO)  /  circular dichroism (CD)  /  high performance gel permeation chromatography (HPGPC)  /  Trametes Trogii
陈小强, 章银军, 张士康, 励建荣, 金建昌, 郑娜, 杨秀芳. 超高压处理对毛栓菌多酚氧化酶的影响. 高压物理学报, 2012 , 26 (2) : 235 -240 .
Xiao-Qiang CHEN, Yin-Jun ZHANG, Shi-Kang ZHANG, Jian-Rong LI, Jian-Chang JIN, Na ZHENG, Xiu-Fang YANG. Effect of High Pressure Processing on Polyphenol Oxidase from Trametes Trogii[J]. Chinese Journal of High Pressure Physics, 2012 , 26 (2) : 235 -240 .
基金
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  • 科技部农业科技成果转化基金(2010GB24420685)
  • 中华全国供销合作总社杭州茶叶研究院资助项目(2011YJ03)
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2012年第26卷第2期
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文章信息
  • 接收时间：2010-11-17
  • 首发时间：2026-11-05
  • 出版时间：2012-04-15
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出版历史
  • 收稿日期：2010-11-17
  • 修回日期：2011-02-17
基金
科技部农业科技成果转化基金(2010GB24420685)
中华全国供销合作总社杭州茶叶研究院资助项目(2011YJ03)
作者信息
    1.中华全国供销合作总社杭州茶叶研究院，浙江 杭州　310016
    2.浙江工商大学食品与生物工程学院，浙江 杭州　310035
    3.浙江工业大学生物与环境工程学院，浙江 杭州　310014
    4.浙江树人大学生物与环境工程学院，浙江 杭州　310015
    5.浙江大学化学工程联合国家重点实验室，浙江 杭州　310027
    6.渤海大学，辽宁 锦州　121013

通讯作者:

张士康（1965—），男，博士，高级工程师，主要从事农副产品加工研究. E-mail：
励建荣（1964—），男，博士，教授，主要从事生鲜食品贮藏加工研究. E-Mail：
金建昌（1978—），男，博士，讲师，主要从事天然产物研究. E-mail：
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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