高压物理学报

高静水压诱导水稻产生突变的研究

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李桂双 ¹^,² , 白成科 ² , 段俊 ¹ , 彭长连 ³

高压物理学报 | 2006,20(4): 421-428

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高压物理学报 | 2006 , 20 (4) : 421 -428

高静水压诱导水稻产生突变的研究

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李桂双¹^,², 白成科², 段俊¹, 彭长连³

作者信息

^1.中国科学院华南植物园，广东广州　510650

^2.陕西师范大学生命科学学院，陕西西安　710062

^3.华南师范大学生命科学学院，广东广州　510631

通讯作者:

段俊，男，博士生导师，主要研究方向为作物遗传育种与生物技术. E-mail：duanj@scib.ac.cn

作者简介:

李桂双（1975—），女，博士研究生. E-mail：guishuangli@scbg.ac.cn

Research on Mutation in Rice (Oryza Sativa L.) Induced by High Hydrostatic Pressure

Gui-Shuang LI¹^,², Cheng-Ke BAI², Jun DUAN¹, Chang-Lian PENG³

Affiliations

^1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China

^2.College of Life Sciences, Shaanxi Normal University, Shaanxi Xi'an 710062, China

^3.College of Life Sciences, South China Normal University, Guangzhou 510640, China

出版时间: 2006-12-05

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摘要

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以78 MPa高静水压力处理已浸泡10 h的水稻（Oryza sativa L.）粤香占、粤丰占和测64种子12 h，成苗后移栽至大田，对其处理当代和后代的农艺性状进行调查和分析，结果表明，处理当代中，粤香占、粤丰占和测64的穗粒数分别增加了5.2%、1.2%和17.0%，且测64达到显著性差异水平；测64处理的生育期延长10 d，而粤香占和粤丰占则分别早熟5 d和2 d。在处理的当代和后代中出现了可遗传的突变体，突变性状中既有质量性状也有数量性状，其中紫色叶鞘和穗下节伸长属于质量性状突变，株高、粒长和穗粒数等属于数量性状突变。突变频率因品种不同而存在差异，表现为：测64>粤丰占>粤香占。通过4年6代的筛选，共获得近100份包括叶、茎、穗、粒、育性和生育期等性状发生明显变异的突变体，已稳定的突变株系22个，其中7个突变株系的综合性状明显较对照好，具有良好的应用前景。

关键词

高静水压 / 水稻 / 突变

Abstract

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Seeds of three rice (Oryza sativa L.) cultivars, Yuexiangzhan, Yuefengzhan and Ce 64, were soaked for 10 h, and then were treated by high hydrostatic pressure (78 MPa) for 12 h. The seedlings of high-pressure treatment and control were transplanted in the field after sowing of 30 d. Mutation type of characters and selection of excellent varieties were investigated. The results indicated that: (1) In M₁ generation, grain numbers per panicle of Yuexiangzhan, Yuefengzhan and Ce 64 treated by high hydrostatic pressure (HHP) increased by 5.2%, 1.2% and 17.0%, respectively. Ce 64 delayed 10 d but Yuexiangzhan and Yuefengzhan advanced 5 d and 2 d in period of duration. (2) There were quality and quantity traits among the mutation traits by HHP. For instance, dominant mutation in leaf sheath made green leaf sheath become purple leaf sheath, while recessive mutation in the node length below panicle made node length of treatment longer than that of control. Mutation in plant height, awn length and grain numbers per panicle belonged to mutation of quantity traits. (3) Mutation frequency and the time from mutant to stable mutation lines varied among species. Mutation frequency of Ce 64 in M₁ generation was 8% while that of Yuefengzhan and Yuexiangzhan was 1‰ and 5‰, respectively. (4) Currently one hundred mutants were found in the field. Twenty-two mutants had no significant separations and seven of them displayed remarkable advantages in comprehensive traits than their control.

Key words

high hydrostatic pressure / rice / mutation

引用本文

李桂双, 白成科, 段俊, 彭长连. 高静水压诱导水稻产生突变的研究. 高压物理学报, 2006 , 20 (4) : 421 -428 .

Gui-Shuang LI, Cheng-Ke BAI, Jun DUAN, Chang-Lian PENG. Research on Mutation in Rice (Oryza Sativa L.) Induced by High Hydrostatic Pressure[J]. Chinese Journal of High Pressure Physics, 2006 , 20 (4) : 421 -428 .

基金

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国家自然科学基金(30471002)
广东省自然科学基金重点项目(05103637)

参考文献

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2006年第20卷第4期

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接收时间：2005-09-04
首发时间：2026-11-01
出版时间：2006-12-05

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收稿日期：2005-09-04
修回日期：2005-10-24

基金

国家自然科学基金(30471002)

广东省自然科学基金重点项目(05103637)

作者信息

^1.中国科学院华南植物园，广东广州　510650

^2.陕西师范大学生命科学学院，陕西西安　710062

^3.华南师范大学生命科学学院，广东广州　510631

通讯作者:

段俊，男，博士生导师，主要研究方向为作物遗传育种与生物技术. E-mail：duanj@scib.ac.cn

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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