高压物理学报

高压处理后水稻抗氧化酶活性及对逆境胁迫的响应

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白成科 ¹^,² , 李桂双 ¹ , 段俊 ¹ , 彭长连 ³ , 翁克难 ⁴ , 徐世平 ⁴

高压物理学报 | 2005,19(3): 235-240

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高压物理学报 | 2005 , 19 (3) : 235 -240

高压处理后水稻抗氧化酶活性及对逆境胁迫的响应

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白成科¹^,², 李桂双¹, 段俊¹, 彭长连³, 翁克难⁴, 徐世平⁴

作者信息

^1.中国科学院华南植物园，广东广州　510650

^2.陕西师范大学生命科学学院，陕西西安　710062

^3.华南师范大学生命科学学院，广东广州，510631

^4.中国科学院广州地球化学研究所，广东广州　510640

通讯作者:

段俊，男，博士生导师，主要研究方向：作物遗传育种与生物技术. E-mail：duanj@scbg.ac.cn

作者简介:

白成科（1976—），男，博士，讲师，E-mail：baichengke@snnu.edu.cn

Activities of Antioxidative Enzymes and the Responds to Cold Stress of Rice Treated by High Hydrostatic Pressure

Cheng-Ke BAI¹^,², Gui-Shuang LI¹, Jun DUAN¹, Chang-Lian PENG³, Ke-Nan WENG⁴, Shi-Ping XU⁴

Affiliations

^1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China

^2.College of Life Sciences, Shaanxi Normal University, Xi'an 710062, China

^3.College of Life Sciences, South China Normal University, Guangzhou 510631, China

^4.Guangzhou Institute of Geochemistry, Chinese Academy of Sciences, Guangzhou 510640, China

出版时间: 2005-09-05

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摘要

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对经高静水压处理的水稻粤香占（Oryza sativa L. cv. Yuexiangzhan）种子播种后的植株进行了抗氧化酶活性的测定和对逆境胁迫响应的研究。结果表明，高压处理抑制了水稻生长早期（12 d）色素和蛋白质含量的增加，并使早期抗氧化酶活性表现较对照低。随着播种天数增加，经高压处理的材料中的色素、蛋白质含量不同程度地增加，抗氧化酶活性发生改变，至播种后32 d时，高压处理的叶绿素含量和可溶性蛋白含量都高于对照粤香占。在自然低温条件下，经高压（干压、湿压）处理的植株其Rubisco大、小亚基含量和光合色素含量较高，抗光抑制能力增强，具一定的耐低温性。

关键词

水稻 / 高静水压 / 抗氧化 / 胁迫

Abstract

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Rice seeds (Oryza sativa L. cv. Yuexiangzhan) were soaked for 10 h or not soaked, then exposed to 75 MPa high hydrostatic pressure (HHP) for 12 h. The seeds treated by HHP and their control were sowed in the field. Activities of antioxidative enzymes (SOD, AsA-POD) in the leaves, Rubisco content, F_v/F_m and Φ_PSⅡ were measured to evaluate the effect of HHP on the growth and development of rice. Results indicated that HHP inhibited the increasing of pigment and protein content in the early growth stage (12 d). Activities of antioxidative enzymes were lower than that of the control at the moment. With days after sowing increasing, the content of pigment and protein in the treatment were higher than that of the control (Yuexiangzhan). Antioxidative ability of rice treated by HHP was stronger than that of the control at the 32th days after sowing. The content of Rubisco, including Rubisco large subunit and Rubisco small subunit, was higher in the treated rice than that of the control at the natural low temperature.F_v/F_m and Φ_PSⅡ of treatment and control decreased at noon but can recover at evening, and recovery of treatment was better than that of the control. These results suggested that difference in growth between the treatment and control was due to the effect of HHP on the activities of antioxidative enzymes of rice possibly. Furthermore, HHP may enhance rice's resistance to low temperature.

Key words

rice / high hydrostatic pressure / antioxidative / stress

引用本文

白成科, 李桂双, 段俊, 彭长连, 翁克难, 徐世平. 高压处理后水稻抗氧化酶活性及对逆境胁迫的响应. 高压物理学报, 2005 , 19 (3) : 235 -240 .

Cheng-Ke BAI, Gui-Shuang LI, Jun DUAN, Chang-Lian PENG, Ke-Nan WENG, Shi-Ping XU. Activities of Antioxidative Enzymes and the Responds to Cold Stress of Rice Treated by High Hydrostatic Pressure[J]. Chinese Journal of High Pressure Physics, 2005 , 19 (3) : 235 -240 .

基金

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国家自然科学基金(30471002)
中国科学院农业项目办公室重点资助项目和国家重大基础研究发展项目(G1998010100)

参考文献

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2005年第19卷第3期

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接收时间：2004-08-05
首发时间：2026-10-31
出版时间：2005-09-05

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出版历史

收稿日期：2004-08-05
修回日期：2004-11-26

基金

国家自然科学基金(30471002)

中国科学院农业项目办公室重点资助项目和国家重大基础研究发展项目(G1998010100)

作者信息

^1.中国科学院华南植物园，广东广州　510650

^2.陕西师范大学生命科学学院，陕西西安　710062

^3.华南师范大学生命科学学院，广东广州，510631

^4.中国科学院广州地球化学研究所，广东广州　510640

通讯作者:

段俊，男，博士生导师，主要研究方向：作物遗传育种与生物技术. E-mail：duanj@scbg.ac.cn

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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