Article(id=1322942664483676715, tenantId=1146029695717560320, journalId=1255847867265597444, issueId=1322941937514316514, articleNumber=null, orderNo=null, doi=null, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1196179200000, receivedDateStr=2007-11-28, revisedDate=1206633600000, revisedDateStr=2008-03-28, acceptedDate=null, acceptedDateStr=null, onlineDate=1793343710299, onlineDateStr=2026-10-30, pubDate=1228406400000, pubDateStr=2008-12-05, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793343710299, onlineIssueDateStr=2026-10-30, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793343710299, creator=13701087609, updateTime=1793343710299, updator=13701087609, issue=Issue{id=1322941937514316514, tenantId=1146029695717560320, journalId=1255847867265597444, year='2008', volume='22', issue='4', pageStart='337', pageEnd='448', issueExtLink='null', onlineDate='null', pubDate='1228406400000', pubDateStr='2008-12-05', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1793343536975, creator='13701087609', updateTime=1793343536975, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext=null, issueFiles=null, downloadFileDto=null}, startPage=370, endPage=376, ext={EN=ArticleExt(id=1322942666153009708, articleId=1322942664483676715, tenantId=1146029695717560320, journalId=1255847867265597444, language=EN, title=Construction and Analysis of SSH-cDNA Library Induced by High Pressure in Rice Seeds during Germination, columnId=null, journalTitle=Chinese Journal of High Pressure Physics, columnName=null, runingTitle=null, highlight=null, articleAbstract=

In order to study the effect of expression pattern of high hydrostatic pressure on high plant, a SSH-cDNA library was constructed with rice germinating seeds in this paper. Suppression subtractive hybridization (SSH) in combination with mirror orientation selection (MOS) was performed using the mRNA from rice germinating seeds treated by 75 MPa high hydrostatic pressure as tester and the mRNA from control (seeds untreated by high hydrostatic pressure) as the driver. 580 recombinant clones were picked through blue/white screening. PCR detection of the recombinant clones showed that the size of inserts mainly ranged from 200 bp to 1 000 bp, and the average size of these inserts were about 575 bp. Among the 580 recombinant clones, 542 clones (93.3%) were identified to be effective single band ones. After sequencing some of the recombinant clones, it was found that the genes induced by high hydrostatic pressure were mainly involved in metabolism, stress response and transcriptional regulation. The research paved the way for studying the gene expression patterns of rice seeds responding to high hydrostatic pressure.

, authors=Xun-Cheng LIU1, 2, Mei ZHANG1, Jun DUAN1, authorsList=Xun-Cheng LIU, Mei ZHANG, Jun DUAN, authorCompany=null, correspAuthors=Jun DUAN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322942666358530605, articleId=1322942664483676715, tenantId=1146029695717560320, journalId=1255847867265597444, language=CN, title=水稻种子萌发期高压诱导的SSH-cDNA文库构建与分析, columnId=null, journalTitle=高压物理学报, columnName=, runingTitle=null, highlight=null, articleAbstract=

为了研究高压处理对高等植物基因表达的影响,以萌发过程中经高压处理后的水稻种子的mRNA作为检测子(Tester),以对照样品的mRNA作为驱动子(Driver),采用抑制差减杂交(SSH)结合镜像选择技术(MOS)构建了高压处理后水稻种子萌发过程中诱导表达的差减cDNA文库,并通过蓝白斑筛选获得了580个重组克隆。菌液PCR检测结果表明,这些克隆中的插入片段的大小主要分布在200~1 000 bp之间,平均大小约为575 bp,其中单一扩增条带的有效重组克隆数为542个,有效的重组克隆率为93.3%。对部分重组克隆进行测序发现,受高压诱导表达的基因主要涉及到代谢、胁迫响应、转录调控等方面。为高压处理后水稻种子基因表达模式变化的研究打下了基础。

, authors=刘勋成1, 2, 张美1, 段俊1, authorsList=刘勋成, 张美, 段俊, authorCompany=null, correspAuthors=段俊, authorNote=

刘勋成(1979—),男,博士研究生,主要从事植物分子生物学研究. E-mail:

, correspAuthorsNote=
段俊,男,博士,研究员,博士生导师,主要从事植物遗传育种与生物技术研究. E-mail:
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=NvXsufya15FMbHk2ixyMMQ==, magXml=Esps6lx/77nqFLr/LCWFMg==, pdfUrl=null, pdf=8gCceQgPzktfv2sNrSPaow==, pdfFileSize=3279591, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=xXsKcOz9U3gi/eE8HEA8fg==, mapNumber=null, fund=null)}, authors=[Author(id=1322943857419231470, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=lxc06981@163.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322943857524089073, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857419231470, language=EN, stringName=Xun-Cheng LIU, firstName=Xun-Cheng, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322943857612169458, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857419231470, language=CN, stringName=刘勋成, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1.中国科学院华南植物园,广东 广州 510650
2.中国科学院研究生院,北京 100039, bio={"content":"

刘勋成(1979—),男,博士研究生,主要从事植物分子生物学研究. E-mail:

"}, bioImg=null, bioContent=

刘勋成(1979—),男,博士研究生,主要从事植物分子生物学研究. E-mail:

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322943857003995367, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=1., ext=[AuthorCompanyExt(id=1322943857016578280, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China), AuthorCompanyExt(id=1322943857024966889, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国科学院华南植物园,广东 广州 510650)]), AuthorCompany(id=1322943857113047274, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=2., ext=[AuthorCompanyExt(id=1322943857121435883, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857113047274, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China), AuthorCompanyExt(id=1322943857134018796, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857113047274, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.中国科学院研究生院,北京 100039)])]), Author(id=1322943857842856180, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322943857918353654, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857842856180, language=EN, stringName=Mei ZHANG, firstName=Mei, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322943857993851127, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857842856180, language=CN, stringName=张美, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.中国科学院华南植物园,广东 广州 510650, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322943857003995367, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=1., ext=[AuthorCompanyExt(id=1322943857016578280, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China), AuthorCompanyExt(id=1322943857024966889, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国科学院华南植物园,广东 广州 510650)])]), Author(id=1322943858073542905, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=duanj@scib.ac.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1322943858136457467, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943858073542905, language=EN, stringName=Jun DUAN, firstName=Jun, middleName=null, lastName=DUAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322943858216149244, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943858073542905, language=CN, stringName=段俊, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.中国科学院华南植物园,广东 广州 510650, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322943857003995367, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=1., ext=[AuthorCompanyExt(id=1322943857016578280, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China), AuthorCompanyExt(id=1322943857024966889, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国科学院华南植物园,广东 广州 510650)])])], keywords=[Keyword(id=1322943860019699965, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, language=EN, orderNo=1, keyword=high hydrostatic pressure), Keyword(id=1322943860111974654, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, language=EN, orderNo=2, keyword=suppression subtractive hybridization (SSH)), Keyword(id=1322943860191666431, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, language=EN, orderNo=3, keyword=rice), Keyword(id=1322943860267163904, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, language=CN, orderNo=1, keyword=高压), Keyword(id=1322943860334272769, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, language=CN, orderNo=2, keyword=抑制差减杂交), Keyword(id=1322943860409770242, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, language=CN, orderNo=3, keyword=水稻)], refs=[Reference(id=1322943860938252550, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[1], rfOrder=0, authorNames=null, journalName=null, refType=null, unstructuredReference=Winter R. High Pressure Effects in Molecular Biophysics[A]//Proceedings of the International School of Physics “Enrico Fermi”, Course CXLVII on High Pressure Phenomena[C]. Amsterdam: IOS, 2002: 413-453., articleTitle=null, refAbstract=null), Reference(id=1322943861034721543, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=null, journalName=null, refType=null, unstructuredReference=Kaarniranta K, Elo M, Sironen R, et al. Hsp70 Accumulation in Chondrocytic Cells Exposed to High Continuous Hydrostatic Pressure Coincides with mRNA Stabilization Rather than Transcriptional Activation[J]. Proc Natl Acad Sci USA, 1998, 95: 2319-2324., articleTitle=null, refAbstract=null), Reference(id=1322943861110219016, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=null, journalName=null, refType=null, unstructuredReference=Dixon D R, Pruski A M, Dixon L R J, et al. The Effects of Hydrostatic Pressure Change on DNA Integrity and Embryonic Development in the Hydrothermal-Vent Mussel Bathymodiolus Azoricus: Implications for Future Deep-Sea Mutagenicity Studies[J]. Mutation Research, 2004, 552: 235-246., articleTitle=null, refAbstract=null), Reference(id=1322943861181522185, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=null, journalName=null, refType=null, unstructuredReference=Iwahashi H, Odani M, Ishidou E, et al. Adaptation of Saccharomyces Cerevisiae to High Hydrostatic Pressure Causing Growth Inhibition[J]. FEBS Letters, 2005, 579: 2847-2852., articleTitle=null, refAbstract=null), Reference(id=1322943861261213962, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=null, journalName=null, refType=null, unstructuredReference=Fernandes P M, Domitrovic T, Kao C M, et al. Genomic Expression Pattern in Saccharomyces Cerevisiae Cells in Response to High Hydrostatic Pressure[J]. FEBS Letters, 2004, 556: 153-160., articleTitle=null, refAbstract=null), Reference(id=1322943861361877259, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=null, journalName=null, refType=null, unstructuredReference=Vogel R F, Pavlovic M, Hormann S, et al. High Pressure-Sensitive Gene Expression in Lactobacillus Sanfranciscensis[J]. Braz J Med Biol Res, 2005, 38: 1247-1252., articleTitle=null, refAbstract=null), Reference(id=1322943861466734860, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=null, journalName=null, refType=null, unstructuredReference=Li G S, Bai C K, Duan J, et a1. Research on Mutation in Rice (Oryza Sativa L.) Induced by High Hydrostatic Pressure[J]. Chinese Journal of High Pressure Physics, 2006, 20(4): 421-428. (in Chinese), articleTitle=null, refAbstract=null), Reference(id=1322943861584175373, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[7], rfOrder=7, authorNames=null, journalName=null, refType=null, unstructuredReference=李桂双, 白成科, 段俊, 等. 高静水压诱导水稻产生突变的研究[J]. 高压物理学报, 2006, 20(4): 421-428., articleTitle=null, refAbstract=null), Reference(id=1322943861672255758, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=8, authorNames=null, journalName=null, refType=null, unstructuredReference=Rebrikov D V, Britanova O V, Gurskaya N G, et al. Mirror Orientation Selection (MOS): A Method for Eliminating False Positive Clones from Libraries Generated by Suppression Subtractive Hybridization[J]. Nucleic Acids Res, 2000, 28: E90., articleTitle=null, refAbstract=null), Reference(id=1322943861768724751, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=9, authorNames=null, journalName=null, refType=null, unstructuredReference=Sambrook J, Russell D. Molecular Clone: A Laboratory Manual (3rd ed)[M]. Translated by Huang P T. Beijing: Science Press, 2002: 93-96. (in Chinese) Sambrook J, Russell D., articleTitle=null, refAbstract=null), Reference(id=1322943861881970960, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[9], rfOrder=10, authorNames=null, journalName=null, refType=null, unstructuredReference=分子克隆实验指南(第3版)[M]. 黄培堂, 译. 北京: 科学出版社, 2002: 93-96., articleTitle=null, refAbstract=null), Reference(id=1322943861982634257, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[10], rfOrder=11, authorNames=null, journalName=null, refType=null, unstructuredReference=Ji W, Wright M B, Cai L, et al. Efficacy of SSH PCR in Isolating Differentially Expressed Genes[J]. BMC Genomics, 2002, 20: 3-12., articleTitle=null, refAbstract=null), Reference(id=1322943862074908946, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[11], rfOrder=12, authorNames=null, journalName=null, refType=null, unstructuredReference=Diatchenko L, Lau Y F, Campbell A P, et a1. Suppression Subtractive Hybridization: A Method for Generating Differentially Regulated or Tissue-Specific cDNA Probes and Libraries[J]. Proc Natl Acad Sci USA, 1996, 93(12): 6025-6030., articleTitle=null, refAbstract=null), Reference(id=1322943862167183635, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=13, authorNames=null, journalName=null, refType=null, unstructuredReference=Li G C, Jin L P, Xie K Y, et al. The Principle of Suppression Subtractive Hybridization Technique and Its Application in Plant Gene Isolation[J]. China Biotechnology, 2004, 24(9): 26-34. (in Chinese), articleTitle=null, refAbstract=null), Reference(id=1322943862272041236, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=14, authorNames=null, journalName=null, refType=null, unstructuredReference=李广存, 金黎平, 谢开云, 等. 抑制差减杂交(SSH)技术及其在植物基因分离上的应用[J]. 中国生物工程杂志, 2004, 24(9): 26-34., articleTitle=null, refAbstract=null), Reference(id=1322943862368510229, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[13], rfOrder=15, authorNames=null, journalName=null, refType=null, unstructuredReference=Su P H, Yu S M, Chen C S, et a1. Spatial and Temporal Expression of a Rice Prolamin Gene RP5 Promoter in Transgenic Tobacco and Rice[J]. Plant Physiol, 2001, 158(2): 247-254., articleTitle=null, refAbstract=null), Reference(id=1322943862452396310, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[14], rfOrder=16, authorNames=null, journalName=null, refType=null, unstructuredReference=Mandal S, Mandal R K. Seed Storage Proteins and Approaches for Improvement of Their Nutritional Quality by Genetic Engineering[J]. Current Science, 2000, 79(5): 576-589., articleTitle=null, refAbstract=null), Reference(id=1322943862532088087, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=17, authorNames=null, journalName=null, refType=null, unstructuredReference=Bai C K, Li G S, Duan J, et a1. Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)[J]. Chinese Journal of High Pressure Physics, 2003, 17(4): 283-289. (in Chinese), articleTitle=null, refAbstract=null), Reference(id=1322943862624362776, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[15], rfOrder=18, authorNames=null, journalName=null, refType=null, unstructuredReference=白成科, 李桂双, 段俊, 等. 高静水压对水稻种子萌发及同工酶的影响[J]. 高压物理学报, 2003, 17(4): 283-289., articleTitle=null, refAbstract=null), Reference(id=1322943862695665945, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[16], rfOrder=19, authorNames=null, journalName=null, refType=null, unstructuredReference=McCord J M, Fridovich I. Superoxide Dismutase. An Enzymic Function for Erythrocuprein (Hemocuprein)[J]. J Biol Chem, 1969, 244(3): 6049-6055., articleTitle=null, refAbstract=null), Reference(id=1322943864381776154, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, doi=null, pmid=null, pmcid=null, year=null, volume=null, issue=null, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=20, authorNames=null, journalName=null, refType=null, unstructuredReference=Hwang I K, Eum W S, Yoo K-Y, et al. Copper Chaperone for Cu, Zn-SOD Supplement Potentiates the Cu, Zn-SOD Function of Neuroprotective Effects against Ischemic Neuronal Damage in the Gerbil Hippocampus[J]. Free Radic Biol Med, 2005, 39(3): 392-402., articleTitle=null, refAbstract=null)], funds=[Fund(id=1322943860585931011, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, awardId=05103637, language=CN, fundingSource=广东省自然科学基金重点项目(05103637), fundOrder=null, country=null), Fund(id=1322943860674011396, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, awardId=0553011001, language=CN, fundingSource=华南植物园科研启动经费(0553011001), fundOrder=null, country=null), Fund(id=1322943860745314565, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, awardId=2006B20201016, language=CN, fundingSource=广东省科技攻关计划项目(2006B20201016), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1322943857003995367, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=1., ext=[AuthorCompanyExt(id=1322943857016578280, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China), AuthorCompanyExt(id=1322943857024966889, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国科学院华南植物园,广东 广州 510650)]), AuthorCompany(id=1322943857113047274, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=2., ext=[AuthorCompanyExt(id=1322943857121435883, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857113047274, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China), AuthorCompanyExt(id=1322943857134018796, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857113047274, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.中国科学院研究生院,北京 100039)])], figs=null, attaches=null, journal=Journal(id=1255846718311514113, delFlag=0, nameCn=高压物理学报, nameEn=Chinese Journal of High Pressure Physics, nameHistory1=null, nameHistory2=null, issn=1000-5773, eissn=null, cn=51-1147/O4, coden=null, periodic=0, language=CN, oaType=null, ccby=null, superviseOffice=null, ownerOffice=null, pubOffice=null, editorOffice=null, officeType=null, aims=null, clcCode=null, officeProv=null, officeCity=null, officeAddr=null, officeZip=null, officeEmail=null, officePhone=null, editDirector=null, officeDirector=null, officeDirectorPhone=null, officeStaffNum=null, officeEmpNum=null, coverPicUrl=KWePOcsfwWv2cMGG6VIsRw==, journalPrice=null, startedYear=null, abbrevIsoEn=Chinese Journal of High Pressure Physics, journalRemark=null, publicationField=null, createdTime=1777346790154, updatedTime=1788952099200, createdBy=18614031015, updatedBy=13041195026, firstLetterCn=G, firstLetterEn=G, subjectCode=Science, subjectName=null, subjectCodeEn=Science, subjectNameEn=null, picCn=KWePOcsfwWv2cMGG6VIsRw==, picEn=yfL33fksqTlch2QW39glNg==, jcr=null, cjcr=null, exts=[JournalExt(id=1304522912573182860, language=CN, name=高压物理学报, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788952099221, updatedTime=1788952099221, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://gywlxbauthor.manuscriptcloud.com/, submissionEditorUrl=https://gywlxbeditor.manuscriptcloud.com/, submissionReviewUrl=https://gywlxbauthor.manuscriptcloud.com/, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""}), JournalExt(id=1304522912619320205, language=EN, name=Chinese Journal of High Pressure Physics, nameHistory1=null, nameHistory2=null, managedBy=, sponsoredBy=, publishedBy=, editorOffice=, officeProv=null, officeCity=null, officeAddr=, officeZip=, editDirector=, officeDirector=null, officePhone=null, coverPicUrl=null, journalRemark=, submitArticleUrl=null, websiteUrl=, createdTime=1788952099232, updatedTime=1788952099232, createdBy=13041195026, updatedBy=13041195026, submissionGuidelinesUrl=, submissionAuthorUrl=https://gywlxbauthor.manuscriptcloud.com/, submissionEditorUrl=https://gywlxbeditor.manuscriptcloud.com/, submissionReviewUrl=https://gywlxbauthor.manuscriptcloud.com/, submissionCeEditorUrl=, submissionAeEditorUrl=, option={"copyright":""})], databaseList=null, tenantJournalId=1255847867265597444, websiteList=[Website(id=1255847989789208939, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1255847867265597444, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/gywlxb/CN, language=CN, createTime=1777347093294, createBy=18614031015, updateTime=1777347319285, updateBy=18614031015, name=高压物理学报-中文, tplId=1146099689490845704, title=高压物理学报, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1255849119986364878, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=articleTextType, value=kx, createTime=1777347362753, updateTime=1777347362753, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849119952810443, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=banner, value=null, createTime=1777347362745, updateTime=1777347362745, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849120045085137, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=grayFlag, value=0, createTime=1777347362767, updateTime=1777347362767, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849119944421834, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=logo, value=https://castjournals.cast.org.cn/joweb/gywlxb/CN/file/pic?fileId=5J9nsSD+WX0mc+nIBFk2Hg==, createTime=1777347362743, updateTime=1777347362743, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849120368046547, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=minRunFlag, value=0, createTime=1777347362844, updateTime=1777347362844, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849119973781965, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/gywlxb/CN/file/pic, createTime=1777347362750, updateTime=1777347362750, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849120347075026, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=silenceFlag, value=0, createTime=1777347362839, updateTime=1777347362839, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849119965393356, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_cn_619/, createTime=1777347362748, updateTime=1777347362748, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849120007336399, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=themeColor, value=null, createTime=1777347362758, updateTime=1777347362758, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849120019919312, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989789208939, code=themeStyle, value=null, createTime=1777347362761, updateTime=1777347362761, creator=18614031015, updator=18614031015)]), Website(id=1255847989940203908, webName=null, webTitle=null, webDomain=null, webCopyrigh=null, webIpcNo=null, seoTitle=null, seoKeywords=null, seoDescription=null, tenantJournalId=null, journalId=1255847867265597444, journalNameCn=null, journalNameEn=null, grayFlag=null, tenantId=1146029695717560320, platformId=null, journalGroupId=null, journalGroupNameCn=null, journalGroupNameEn=null, type=1, domain=https://castjournals.cast.org.cn/joweb/gywlxb/EN, language=EN, createTime=1777347093329, createBy=18614031015, updateTime=1777347316256, updateBy=18614031015, name=高压物理学报-英文, tplId=1146101810881728533, title=Chinese Journal of High Pressure Physics, delFlag=0, indexPage=/home, props=[WebsiteProps(id=1255849089909010882, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=articleTextType, value=kx, createTime=1777347355582, updateTime=1777347355582, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089871262143, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=banner, value=null, createTime=1777347355573, updateTime=1777347355573, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089934176709, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=grayFlag, value=0, createTime=1777347355588, updateTime=1777347355588, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089858679230, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=logo, value=https://castjournals.cast.org.cn/joweb/gywlxb/EN/file/pic?fileId=5J9nsSD+WX0mc+nIBFk2Hg==, createTime=1777347355570, updateTime=1777347355570, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089950953927, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=minRunFlag, value=0, createTime=1777347355592, updateTime=1777347355592, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089888039361, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=picServerUrl, value=https://castjournals.cast.org.cn/joweb/gywlxb/EN/file/pic, createTime=1777347355577, updateTime=1777347355577, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089942565318, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=silenceFlag, value=0, createTime=1777347355590, updateTime=1777347355590, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089879650752, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=staticResourcePath, value=https://castjournals.cast.org.cn/joweb/cast_kjdb_en_623/, createTime=1777347355575, updateTime=1777347355575, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089917399491, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=themeColor, value=null, createTime=1777347355584, updateTime=1777347355584, creator=18614031015, updator=18614031015), WebsiteProps(id=1255849089925788100, tenantId=1146029695717560320, journalId=null, journalGroupId=null, siteId=1255847989940203908, code=themeStyle, value=null, createTime=1777347355586, updateTime=1777347355586, creator=18614031015, updator=18614031015)])], journalTitle=高压物理学报, weixinUrl=null, journalUrl=https://www.gywlxb.cn, iacademicId=null, status=1, seqNo=null, journalTitleEn=Chinese Journal of High Pressure Physics, journalPhotoCn=KWePOcsfwWv2cMGG6VIsRw==, journalPhotoEn=yfL33fksqTlch2QW39glNg==, journalFirstLetter=G, journalRecommend=null, journalNew=null, journalCollection=null, jcrJf=null, cjcrJf=null, jcrJfStr=null, cjcrJfStr=null, submissionFirstDecision=null, sciSubjectClassification=null, casSubjectClassification=null, citeScore=null, totalCitationFrequency=null, icpCode=null, psCode=null, advertisingLicenseCode=null, copyrightInformation=null, country=null, option=, provinceCode=null, provinceName=null, collectFlag=false, interPubPlatform=, interPubPlatformUrl=null), detailUrlCn=https://castjournals.cast.org.cn/joweb/gywlxb/CN/Y2008/V22/I4/370, detailUrlEn=https://castjournals.cast.org.cn/joweb/gywlxb/EN/Y2008/V22/I4/370, pdfUrlCn=https://castjournals.cast.org.cn/joweb/gywlxb/CN/PDF/Y2008/V22/I4/370, pdfUrlEn=https://castjournals.cast.org.cn/joweb/gywlxb/EN/PDF/Y2008/V22/I4/370, aliStartDate=0, aliEndDate=0, collectionFlag=false, citedCount=null, citedUrl=null, previewStatus=0, delFlag=0, hasFullText=0, orderTime=1228406400000, fullTextJson=null, articleText=null, reference=null)
收藏切换
水稻种子萌发期高压诱导的SSH-cDNA文库构建与分析
收藏切换
PDF下载
刘勋成 1, 2 , 张美 1 , 段俊 1
高压物理学报 | 2008,22(4): 370-376
收起
收藏切换
高压物理学报 | 2008 , 22 (4) : 370 -376
水稻种子萌发期高压诱导的SSH-cDNA文库构建与分析
全屏
[Author(id=1322943857419231470, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=lxc06981@163.com, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322943857524089073, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857419231470, language=EN, stringName=Xun-Cheng LIU, firstName=Xun-Cheng, middleName=null, lastName=LIU, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China
2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322943857612169458, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857419231470, language=CN, stringName=刘勋成, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, address=1.中国科学院华南植物园,广东 广州 510650
2.中国科学院研究生院,北京 100039, bio={"content":"

刘勋成(1979—),男,博士研究生,主要从事植物分子生物学研究. E-mail:

"}, bioImg=null, bioContent=

刘勋成(1979—),男,博士研究生,主要从事植物分子生物学研究. E-mail:

, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322943857003995367, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=1., ext=[AuthorCompanyExt(id=1322943857016578280, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China), AuthorCompanyExt(id=1322943857024966889, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国科学院华南植物园,广东 广州 510650)]), AuthorCompany(id=1322943857113047274, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=2., ext=[AuthorCompanyExt(id=1322943857121435883, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857113047274, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China), AuthorCompanyExt(id=1322943857134018796, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857113047274, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.中国科学院研究生院,北京 100039)])]), Author(id=1322943857842856180, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, orderNo=1, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1322943857918353654, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857842856180, language=EN, stringName=Mei ZHANG, firstName=Mei, middleName=null, lastName=ZHANG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322943857993851127, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943857842856180, language=CN, stringName=张美, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.中国科学院华南植物园,广东 广州 510650, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322943857003995367, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=1., ext=[AuthorCompanyExt(id=1322943857016578280, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China), AuthorCompanyExt(id=1322943857024966889, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国科学院华南植物园,广东 广州 510650)])]), Author(id=1322943858073542905, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, orderNo=2, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=duanj@scib.ac.cn, emailSecond=null, emailThird=null, correspondingAuthor=1, authorType=1, ext={EN=AuthorExt(id=1322943858136457467, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943858073542905, language=EN, stringName=Jun DUAN, firstName=Jun, middleName=null, lastName=DUAN, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1322943858216149244, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, authorId=1322943858073542905, language=CN, stringName=段俊, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, address=1.中国科学院华南植物园,广东 广州 510650, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null)}, companyList=[AuthorCompany(id=1322943857003995367, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, xref=1., ext=[AuthorCompanyExt(id=1322943857016578280, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China), AuthorCompanyExt(id=1322943857024966889, tenantId=1146029695717560320, journalId=1255847867265597444, articleId=1322942664483676715, companyId=1322943857003995367, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.中国科学院华南植物园,广东 广州 510650)])])]
刘勋成1, 2 , 张美1, 段俊1
作者信息
  • 1.中国科学院华南植物园,广东 广州 510650
  • 2.中国科学院研究生院,北京 100039
通讯作者:
段俊,男,博士,研究员,博士生导师,主要从事植物遗传育种与生物技术研究. E-mail:
作者简介:

刘勋成(1979—),男,博士研究生,主要从事植物分子生物学研究. E-mail:

Construction and Analysis of SSH-cDNA Library Induced by High Pressure in Rice Seeds during Germination
Xun-Cheng LIU1, 2 , Mei ZHANG1, Jun DUAN1
Affiliations
  • 1.South China Botanic Garden, Chinese Academy of Sciences, Guangzhou 510650, China
  • 2.Graduate University of Chinese Academy of Sciences, Beijing 100039, China
出版时间: 2008-12-05
文章导航
收藏切换

为了研究高压处理对高等植物基因表达的影响,以萌发过程中经高压处理后的水稻种子的mRNA作为检测子(Tester),以对照样品的mRNA作为驱动子(Driver),采用抑制差减杂交(SSH)结合镜像选择技术(MOS)构建了高压处理后水稻种子萌发过程中诱导表达的差减cDNA文库,并通过蓝白斑筛选获得了580个重组克隆。菌液PCR检测结果表明,这些克隆中的插入片段的大小主要分布在200~1 000 bp之间,平均大小约为575 bp,其中单一扩增条带的有效重组克隆数为542个,有效的重组克隆率为93.3%。对部分重组克隆进行测序发现,受高压诱导表达的基因主要涉及到代谢、胁迫响应、转录调控等方面。为高压处理后水稻种子基因表达模式变化的研究打下了基础。

高压  /  抑制差减杂交  /  水稻

In order to study the effect of expression pattern of high hydrostatic pressure on high plant, a SSH-cDNA library was constructed with rice germinating seeds in this paper. Suppression subtractive hybridization (SSH) in combination with mirror orientation selection (MOS) was performed using the mRNA from rice germinating seeds treated by 75 MPa high hydrostatic pressure as tester and the mRNA from control (seeds untreated by high hydrostatic pressure) as the driver. 580 recombinant clones were picked through blue/white screening. PCR detection of the recombinant clones showed that the size of inserts mainly ranged from 200 bp to 1 000 bp, and the average size of these inserts were about 575 bp. Among the 580 recombinant clones, 542 clones (93.3%) were identified to be effective single band ones. After sequencing some of the recombinant clones, it was found that the genes induced by high hydrostatic pressure were mainly involved in metabolism, stress response and transcriptional regulation. The research paved the way for studying the gene expression patterns of rice seeds responding to high hydrostatic pressure.

high hydrostatic pressure  /  suppression subtractive hybridization (SSH)  /  rice
刘勋成, 张美, 段俊. 水稻种子萌发期高压诱导的SSH-cDNA文库构建与分析. 高压物理学报, 2008 , 22 (4) : 370 -376 .
Xun-Cheng LIU, Mei ZHANG, Jun DUAN. Construction and Analysis of SSH-cDNA Library Induced by High Pressure in Rice Seeds during Germination[J]. Chinese Journal of High Pressure Physics, 2008 , 22 (4) : 370 -376 .
  • 广东省自然科学基金重点项目(05103637)
  • 华南植物园科研启动经费(0553011001)
  • 广东省科技攻关计划项目(2006B20201016)
参考文献 引证文献
排序方式:
[1]
Winter R. High Pressure Effects in Molecular Biophysics[A]//Proceedings of the International School of Physics “Enrico Fermi”, Course CXLVII on High Pressure Phenomena[C]. Amsterdam: IOS, 2002: 413-453.
[2]
Kaarniranta K, Elo M, Sironen R, et al. Hsp70 Accumulation in Chondrocytic Cells Exposed to High Continuous Hydrostatic Pressure Coincides with mRNA Stabilization Rather than Transcriptional Activation[J]. Proc Natl Acad Sci USA, 1998, 95: 2319-2324.
[3]
Dixon D R, Pruski A M, Dixon L R J, et al. The Effects of Hydrostatic Pressure Change on DNA Integrity and Embryonic Development in the Hydrothermal-Vent Mussel Bathymodiolus Azoricus: Implications for Future Deep-Sea Mutagenicity Studies[J]. Mutation Research, 2004, 552: 235-246.
[4]
Iwahashi H, Odani M, Ishidou E, et al. Adaptation of Saccharomyces Cerevisiae to High Hydrostatic Pressure Causing Growth Inhibition[J]. FEBS Letters, 2005, 579: 2847-2852.
[5]
Fernandes P M, Domitrovic T, Kao C M, et al. Genomic Expression Pattern in Saccharomyces Cerevisiae Cells in Response to High Hydrostatic Pressure[J]. FEBS Letters, 2004, 556: 153-160.
[6]
Vogel R F, Pavlovic M, Hormann S, et al. High Pressure-Sensitive Gene Expression in Lactobacillus Sanfranciscensis[J]. Braz J Med Biol Res, 2005, 38: 1247-1252.
[7]
Li G S, Bai C K, Duan J, et a1. Research on Mutation in Rice (Oryza Sativa L.) Induced by High Hydrostatic Pressure[J]. Chinese Journal of High Pressure Physics, 2006, 20(4): 421-428. (in Chinese)
李桂双, 白成科, 段俊, 等. 高静水压诱导水稻产生突变的研究[J]. 高压物理学报, 2006, 20(4): 421-428.
[8]
Rebrikov D V, Britanova O V, Gurskaya N G, et al. Mirror Orientation Selection (MOS): A Method for Eliminating False Positive Clones from Libraries Generated by Suppression Subtractive Hybridization[J]. Nucleic Acids Res, 2000, 28: E90.
[9]
Sambrook J, Russell D. Molecular Clone: A Laboratory Manual (3rd ed)[M]. Translated by Huang P T. Beijing: Science Press, 2002: 93-96. (in Chinese) Sambrook J, Russell D.
分子克隆实验指南(第3版)[M]. 黄培堂, 译. 北京: 科学出版社, 2002: 93-96.
[10]
Ji W, Wright M B, Cai L, et al. Efficacy of SSH PCR in Isolating Differentially Expressed Genes[J]. BMC Genomics, 2002, 20: 3-12.
[11]
Diatchenko L, Lau Y F, Campbell A P, et a1. Suppression Subtractive Hybridization: A Method for Generating Differentially Regulated or Tissue-Specific cDNA Probes and Libraries[J]. Proc Natl Acad Sci USA, 1996, 93(12): 6025-6030.
[12]
Li G C, Jin L P, Xie K Y, et al. The Principle of Suppression Subtractive Hybridization Technique and Its Application in Plant Gene Isolation[J]. China Biotechnology, 2004, 24(9): 26-34. (in Chinese)
李广存, 金黎平, 谢开云, 等. 抑制差减杂交(SSH)技术及其在植物基因分离上的应用[J]. 中国生物工程杂志, 2004, 24(9): 26-34.
[13]
Su P H, Yu S M, Chen C S, et a1. Spatial and Temporal Expression of a Rice Prolamin Gene RP5 Promoter in Transgenic Tobacco and Rice[J]. Plant Physiol, 2001, 158(2): 247-254.
[14]
Mandal S, Mandal R K. Seed Storage Proteins and Approaches for Improvement of Their Nutritional Quality by Genetic Engineering[J]. Current Science, 2000, 79(5): 576-589.
[15]
Bai C K, Li G S, Duan J, et a1. Effect of High Hydrostatic Pressure on Seeds Germination and Seedling Isoenzyme in Rice (Oryza sativa L.)[J]. Chinese Journal of High Pressure Physics, 2003, 17(4): 283-289. (in Chinese)
白成科, 李桂双, 段俊, 等. 高静水压对水稻种子萌发及同工酶的影响[J]. 高压物理学报, 2003, 17(4): 283-289.
[16]
McCord J M, Fridovich I. Superoxide Dismutase. An Enzymic Function for Erythrocuprein (Hemocuprein)[J]. J Biol Chem, 1969, 244(3): 6049-6055.
[17]
Hwang I K, Eum W S, Yoo K-Y, et al. Copper Chaperone for Cu, Zn-SOD Supplement Potentiates the Cu, Zn-SOD Function of Neuroprotective Effects against Ischemic Neuronal Damage in the Gerbil Hippocampus[J]. Free Radic Biol Med, 2005, 39(3): 392-402.
2008年第22卷第4期
PDF下载
13
4
引用本文
BibTeX
文章信息
  • 接收时间:2007-11-28
  • 首发时间:2026-10-30
  • 出版时间:2008-12-05
补充材料
相关文章
文章信息
作者
出版历史
  • 收稿日期:2007-11-28
  • 修回日期:2008-03-28
基金
广东省自然科学基金重点项目(05103637)
华南植物园科研启动经费(0553011001)
广东省科技攻关计划项目(2006B20201016)
作者信息
    1.中国科学院华南植物园,广东 广州 510650
    2.中国科学院研究生院,北京 100039

通讯作者:

段俊,男,博士,研究员,博士生导师,主要从事植物遗传育种与生物技术研究. E-mail:
参考文献
分享链接
https://castjournals.cast.org.cn/joweb/gywlxb/CN/1322942664483676715
分享至
全文二维码

扫描看全文

引用本文
BibTeX
本文的引用情况
2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
关闭全屏