中南林业科技大学学报

西伯利亚杏MAPK基因家族鉴定及其在低温胁迫下的表达模式分析

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孙春晓 ¹ , 王淋 ² , 敖敦 ¹ , 马明 ¹ , 陈一潇 ¹ , 陈俊兴 ¹ , 夏举国 ¹ , 包文泉 ¹

中南林业科技大学学报 | 生物学 2026,46(8): 216-228

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中南林业科技大学学报 |生物学 2026 , 46 (8) : 216 -228

西伯利亚杏MAPK基因家族鉴定及其在低温胁迫下的表达模式分析

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孙春晓¹, 王淋², 敖敦¹, 马明¹, 陈一潇¹, 陈俊兴¹, 夏举国¹, 包文泉¹

作者信息

^1.内蒙古农业大学　a. 林学院；b. 资源与环境学院，内蒙古　呼和浩特　010000

^2.中国林业科学研究院经济林研究所，河南　郑州　450003

通讯作者:

包文泉（bwq@imau.edu.cn），教授，博士，博士研究生导师。

作者简介:

孙春晓（sunchunxiao0424@163.com），硕士研究生。

Identification and expression analysis of MAPK gene family under low-temperature stress in Prunus sibirica

Chunxiao Sun¹, Lin Wang², Dun Ao¹, Ming Ma¹, Yixiao Chen¹, Junxing Chen¹, Juguo Xia¹, Wenquan Bao¹

Affiliations

^1.a. College of Forestry; b. College of Resources and Environmental Sciences, Inner Mongolia Agricultural University, Hohhot 010000, Inner Mongolia, China

^2.Research Institute of Non-Timber Forestry, Chinese Academy of Forestry, Zhengzhou 450003, Henan, China

出版时间: 2026-08-25 doi: 10.14067/j.cnki.1673-923x.2026.08.021

文章导航

摘要

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【目的】

基于西伯利亚杏全基因组数据，系统鉴定MAPK基因家族成员，分析其生物信息学特征及在低温胁迫下的表达模式，揭示MAPK家族成员参与低温应答的潜在功能。

【方法】

以西伯利亚杏Prunus sibirica基因组数据和转录组数据为基础，通过全基因组鉴定、保守基序分析、进化树构建以及共线性分析等方法，明确MAPK家族基因的基本信息、理化性质、基因结构、系统进化关系及其在低温胁迫下的响应机制。

【结果】

共鉴定出84个西伯利亚杏MAPK基因，其中包括11个PsMAPK基因、10个PsMAPKK基因和63个PsMAPKKK基因，广泛分布于8条染色体上；系统发育分析显示，这些基因分为MAPK、MAPKK和MAPKKK（含MEKK、ZIK、Raf）3个亚家族，各亚家族基序保守。在44个基因的启动子区域中存在低温应答元件。低温胁迫条件下共有14个基因发生差异表达，其中PsMAPK1、PsMAPKKK2等基因的表达量随着温度的降低显著上调。

【结论】

通过对西伯利亚杏MAPK基因家族成员的基因结构、染色体分布及进化关系进行全面分析，明确了MAPK家族成员的理化性质和分子特征。表达分析结果表明，PsMAPK1、PsMAPKK7等14个基因参与了调控低温胁迫响应的关键信号通路。

关键词

MAPK基因 / 全基因组鉴定 / 低温胁迫 / 表达分析 / 西伯利亚杏

Abstract

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【Objective】

To systematically identify the MAPK gene family members based on the whole-genome data of Prunus sibirica, analyze their bioinformatics characteristics and expression patterns under low-temperature stress, and to reveal the potential functions of these family members in response to low-temperature stress.

【Method】

Based on the genomic and transcriptomic data of P. sibirica, methods such as genome-wide identification, conserved motif analysis, phylogenetic tree construction, and syntenic analysis were used to determine the basic information, physicochemical properties, gene structures, phylogenetic relationships, and response mechanisms under low-temperature stress of MAPK family genes.

【Result】

A total of 84 MAPK genes were identified in P. sibirica, including 11 PsMAPK, 10 PsMAPKK and 63 PsMAPKKK genes, which were widely distributed across eight chromosomes. Phylogenetic analysis classified all MAPK family members into three subfamilies: MAPK, MAPKK, and MAPKKK (including MEKK, ZIK, and Raf). Conserved motif analysis revealed that motifs within each subfamily were conserved. Promoter cis-element prediction showed that the promoter regions of 44 genes were enriched in low-temperature-responsive elements. Transcriptome data indicated that 14 MAPK genes were differentially expressed under low-temperature stress, and the expression levels of genes such as PsMAPK1 and PsMAPKKK2 were significantly upregulated with decreasing temperature.

【Conclusion】

Through comprehensive analysis of the gene structure, chromosomal distribution, and phylogenetic relationships of the MAPK gene family members in P. sibirica, the physicochemical properties and molecular characteristics of the MAPK family members were clarified. Further expression analysis indicated that 14 genes, including PsMAPK1 and PsMAPKK7, are involved in important signaling pathways responding to low-temperature stress.

Key words

MAPK gene / genome-wide identification / low-temperature stress / expression analysis / Prunus sibirica

引用本文

孙春晓, 王淋, 敖敦, 马明, 陈一潇, 陈俊兴, 夏举国, 包文泉. 西伯利亚杏MAPK基因家族鉴定及其在低温胁迫下的表达模式分析. 中南林业科技大学学报, 2026 , 46 (8) : 216 -228 . DOI: 10.14067/j.cnki.1673-923x.2026.08.021

Chunxiao Sun, Lin Wang, Dun Ao, Ming Ma, Yixiao Chen, Junxing Chen, Juguo Xia, Wenquan Bao. Identification and expression analysis of MAPK gene family under low-temperature stress in Prunus sibirica[J]. Journal of Central South University of Forestry & Technology, 2026 , 46 (8) : 216 -228 . DOI: 10.14067/j.cnki.1673-923x.2026.08.021

基金

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内蒙古自治区自然科学基金杰出青年基金项目(2024JQ13)
国家自然科学基金地区科学基金项目(32360400)

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2026年第46卷第8期

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文章信息

doi: 10.14067/j.cnki.1673-923x.2026.08.021

接收时间：2025-09-22
首发时间：2026-10-30
出版时间：2026-08-25

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出版历史

收稿日期：2025-09-22
修回日期：2025-12-07

基金

内蒙古自治区自然科学基金杰出青年基金项目(2024JQ13)

国家自然科学基金地区科学基金项目(32360400)

作者信息

^1.内蒙古农业大学　a. 林学院；b. 资源与环境学院，内蒙古　呼和浩特　010000

^2.中国林业科学研究院经济林研究所，河南　郑州　450003

通讯作者:

包文泉（bwq@imau.edu.cn），教授，博士，博士研究生导师。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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