中南林业科技大学学报

油茶根际耐旱促生菌的筛选及其促生潜能分析

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张栩萌 ¹ , 李星语 ¹ , 林婧 ² , 王晓岩 ¹ , 苏喜玉 ¹ , 王慧敏 ¹ , 何苑皞 ¹^,³ , 邓小军 ^1d^,³

中南林业科技大学学报 | 林学 2026,46(9): 120-130

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中南林业科技大学学报 |林学 2026 , 46 (9) : 120 -130

油茶根际耐旱促生菌的筛选及其促生潜能分析

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张栩萌¹, 李星语¹, 林婧², 王晓岩¹, 苏喜玉¹, 王慧敏¹, 何苑皞¹^,³, 邓小军^1d^,³

作者信息

^1.中南林业科技大学　a. 森林有害生物防控湖南省重点实验室；b. 经济林培育与保护教育部重点实验室；c. 森林生物资源与有害生物综合管理湖南省普通高等学校重点实验室；d. 生命与环境科学学院，湖南　长沙　410004

^2.广西壮族自治区国有博白林场，广西　玉林　537600

^3.广西林业科学研究院　a. 国家林业和草原局中南速生材繁育实验室；b. 广西优良用材林资源培育重点实验室，广西　南宁　530002

^1d.中南林业科技大学　生命与环境科学学院，湖南　长沙　410004

通讯作者:

何苑皞（yuanhaohe@csuft.edu.cn），副教授，博士，硕士研究生导师

邓小军（dengxiaojun2008@sina.com），正高级工程师，博士。

作者简介:

张栩萌（1281972147@qq.com），硕士研究生。

Screening of drought tolerant plant growth-promoting rhizobacteria from Camellia oleifera rhizosphere and analysis of their growth-promoting potential

Xumeng Zhang¹, Xingyu Li¹, Jing Lin², Xiaoyan Wang¹, Xiyu Su¹, Huimin Wang¹, Yuanhao He¹^,³, Xiaojun Deng^1d^,³

Affiliations

^1.a. Hunan Provincial Key Laboratory for Control of Forest Diseases and Pests; b. Key Laboratory of Cultivation and Protection for Non-wood Forest Trees, Ministry of Education; c. Key Laboratory of Integrated Management of Forest Biological Resources and Pests of Universities in Hunan Province; d. College of Life and Environmental Sciences, Central South University of Forestry & Technology, Changsha 410004, Hunan, China

^2.Guangxi State-owned Bobai Forest Farm, Yulin 537600, Guangxi, China

^3.a. Zhongnan Fast-growing Wood Breeding Laboratory of National Forestry and Grassland Administration; b. Guangxi Key Laboratory of Superior Timber Tree Resource Cultivation, Guangxi Forestry Research Institute, Nanning 530002, Guangxi, China

^1d.College of Life and Environmental Sciences, Central South University of Forestry & Technology, Changsha 410004, Hunan, China

出版时间: 2026-09-25 doi: 10.14067/j.cnki.1673-923x.2026.09.011

文章导航

摘要

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【目的】

筛选对油茶有良好促生和耐旱效果的细菌，明确其系统分类地位，初步探讨其机理，为提高油茶抗旱性提供新的研究方向。

【方法】

利用聚乙二醇（Polyethylene glycol，PEG）6000模拟干旱胁迫，从干旱环境下长势良好的油茶根际土壤中分离并筛选耐旱菌，测定其固氮、解磷、解钾、分泌吲哚-3-乙酸（Indole-3-acetic acid，IAA）、产铁载体以及产生1-氨基环丙烷-1-羧酸（1-aminocyclopropane-1-carboxylate，ACC）脱氨酶等促生能力，结合形态学、生理生化测定及16 S rDNA序列分析，明确其系统分类地位，通过种子萌发试验验证促生性和抗旱性，并研究其对油茶根部抗氧化酶活性及抗旱相关基因表达的影响。

【结果】

筛选出可耐300 g/L PEG6000干旱胁迫的菌株23株，其中固氮菌21株，解磷菌16株，产IAA菌5株，分泌铁载体菌11株，分泌ACC脱氨酶菌6株；结合耐旱和促生效果，筛选出最佳菌株1J81，结合形态特征、生理生化特征和16 S rDNA测序结果，鉴定为四川肠杆菌Enterobacter sichuanensis；在干旱和非干旱条件下菌株1J81对油茶均有明显的促生效果，同时能够提高油茶根部过氧化氢酶、过氧化物酶、超氧化物歧化酶等酶的活性以及相关抗旱基因POD、SOD1、PR1B1的表达量。

【结论】

来源于油茶根际土壤的四川肠杆菌1J81可作为微生物肥料菌种资源，具有提高油茶抗旱性的潜力。

关键词

油茶 / 耐旱性 / 根际促生菌 / 干旱胁迫

Abstract

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【Objective】

To screen strains with good growth-promoting and drought-tolerant effects on C. oleifera, clarify their systematic classification status, and preliminarily explore their mechanisms, which provides a new research direction for improving the drought resistance of C. oleifera.

【Method】

Drought-tolerant bacteria were isolated and screened from the rhizosphere soil of C. oleifera growing well in a drought environment by using polyethylene glycol (PEG) 6000 to simulate drought stress. Their nitrogen fixation, phosphate solubilization, potassium solubilization, secretion of indole-3-acetic acid (IAA), siderophore and 1-aminocyclopropane-1-carboxylate (ACC) deaminase were determined. Combined with morphological, physiological and biochemical tests and 16S rDNA sequence analysis, the phylogenetic status was clarified. The growth-promoting and drought-resistance were verified by seed germination experiments, and their effects on the activity of antioxidant enzymes and the expression of related resistance genes in the roots of C. oleifera were studied.

【Result】

A total of 23 strains of bacteria that tolerate 300 g/L PEG6000 drought stress were screened, including 21 strains of nitrogen-fixing bacteria, 16 strains of phosphate-solubilizing bacteria, 5 strains of IAA-producing bacteria, 11 strains of siderophore-secreting bacteria and 6 strains of ACC deaminase-secreting bacteria. Combined with drought tolerance and growth-promoting effects, the optimal strain 1J81 is screened and identified as Enterobacter sichuanensis by morphological characteristics, physiological and biochemical tests and 16S rDNA sequencing results. Under both drought and non-drought conditions, strain 1J81 significantly promotes the activities of catalase (CAT), peroxidase (POD), superoxide dismutase (SOD) and other enzymes in the roots of C. oleifera and the expression levels of related resistance genes POD, SOD1 and PR1B1.

【Conclusion】

As a microbial fertilizer strain resource, E. sichuanensis 1J81 derived from the rhizosphere soil of C. oleifera has the potential to improve the drought resistance of C. oleifera.

Key words

Camellia oleifera / drought tolerance / plant growth-promoting rhizobacteria / drought stress

引用本文

张栩萌, 李星语, 林婧, 王晓岩, 苏喜玉, 王慧敏, 何苑皞, 邓小军. 油茶根际耐旱促生菌的筛选及其促生潜能分析. 中南林业科技大学学报, 2026 , 46 (9) : 120 -130 . DOI: 10.14067/j.cnki.1673-923x.2026.09.011

Xumeng Zhang, Xingyu Li, Jing Lin, Xiaoyan Wang, Xiyu Su, Huimin Wang, Yuanhao He, Xiaojun Deng. Screening of drought tolerant plant growth-promoting rhizobacteria from Camellia oleifera rhizosphere and analysis of their growth-promoting potential[J]. Journal of Central South University of Forestry & Technology, 2026 , 46 (9) : 120 -130 . DOI: 10.14067/j.cnki.1673-923x.2026.09.011

基金

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广西自筹经费林业科技项目(2024GXZCLK11)
湖南省教育厅科学研究重点项目(24A0213)

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2026年第46卷第9期

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doi: 10.14067/j.cnki.1673-923x.2026.09.011

接收时间：2025-11-19
首发时间：2026-10-30
出版时间：2026-09-25

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出版历史

收稿日期：2025-11-19
修回日期：2026-04-10

基金

广西自筹经费林业科技项目(2024GXZCLK11)

湖南省教育厅科学研究重点项目(24A0213)

作者信息

^2.广西壮族自治区国有博白林场，广西　玉林　537600

^3.广西林业科学研究院　a. 国家林业和草原局中南速生材繁育实验室；b. 广西优良用材林资源培育重点实验室，广西　南宁　530002

^1d.中南林业科技大学　生命与环境科学学院，湖南　长沙　410004

通讯作者:

何苑皞（yuanhaohe@csuft.edu.cn），副教授，博士，硕士研究生导师

邓小军（dengxiaojun2008@sina.com），正高级工程师，博士。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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