Article(id=1322924404279223240, tenantId=1146029695717560320, journalId=1322258771877134531, issueId=1322924314793750542, articleNumber=null, orderNo=null, doi=10.14067/j.cnki.1673-923x.2026.10.019, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1768320000000, receivedDateStr=2026-01-14, revisedDate=1776873600000, revisedDateStr=2026-04-23, acceptedDate=null, acceptedDateStr=null, onlineDate=1793339356726, onlineDateStr=2026-10-30, pubDate=1792857600000, pubDateStr=2026-10-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793339356726, onlineIssueDateStr=2026-10-30, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793339356726, creator=13701087609, updateTime=1793339356726, updator=13701087609, issue=Issue{id=1322924314793750542, tenantId=1146029695717560320, journalId=1322258771877134531, year='2026', volume='46', issue='10', pageStart='1', pageEnd='256', issueExtLink='null', onlineDate='null', pubDate='1792857600000', pubDateStr='2026-10-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1793339335392, creator='13701087609', updateTime=1793344604640, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322946415693017274, tenantId=1146029695717560320, journalId=1322258771877134531, issueId=1322924314793750542, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322946415693017275, tenantId=1146029695717560320, journalId=1322258771877134531, issueId=1322924314793750542, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=210, endPage=219, ext={EN=ArticleExt(id=1322924404681876425, articleId=1322924404279223240, tenantId=1146029695717560320, journalId=1322258771877134531, language=EN, title=Establishment of an
in vitro rapid propagation system for the rare and endangered tree species
Ormosia microphylla, columnId=null, journalTitle=Journal of Central South University of Forestry & Technology, columnName=null, runingTitle=null, highlight=null, articleAbstract=
【Objective】 Ormosia microphylla is a rare and endangered tree species endemic to China and is listed as a Class I nationally protected wild plant. It possesses high economic and ecological value. Due to limited seed sources, traditional seed propagation methods have a low propagation coefficient; therefore, tissue culture technology has become an important approach for the rapid propagation of O. microphylla. This study aimed to establish an in vitro rapid propagation system for O. microphylla.
【Method】 Mature seeds of O. microphylla were used to establish an aseptic seedling culture system, and the optimal seed sterilization protocol was screened. Bud-bearing stem segments from germinated aseptic seedlings were used as explants, and an L9(33) orthogonal experimental design was employed to determine the optimal medium for shoot induction and proliferation. The effects of different subculture cycles on proliferation were analyzed during the subculture stage. Finally, rooting and transplanting experiments were conducted to screen for the optimal rooting medium and the most suitable transplanting substrate.
【Result】 The optimal sterilization protocol was to retain the seed coat and disinfect the seeds with 2% NaClO for 12 min, under which no contamination occurred, with an aseptic seedling rate of 70.37% and a browning rate of 23.23%. The optimal proliferation medium was MS supplemented with 1.5 mg·L-1 6-BA and 0.15 mg·L-1 IAA, yielding a proliferation coefficient of 5.23 and an average shoot height of 3.59 cm. The third subculture cycle showed the best proliferation performance, with a proliferation coefficient of 5.33. The optimal rooting medium was 1/2 MS supplemented with 0.3 mg·L-1 NAA, resulting in a rooting rate of 93.33%, an average of 3.95 roots per shoot, and an average root length of 3.63 cm. The highest survival rate (95.56%) was achieved when vermiculite∶perlite (1∶1) was used as the substrate.
【Conclusion】 This study established a relatively stable and efficient in vitro rapid propagation system for O. microphylla. Based on experimental investigations of key procedures including explant sterilization, bud induction and proliferation, subculture, rooting and acclimatization, this work provides a theoretical basis and technical support for the germplasm conservation and large-scale propagation of this species.
, authors=Chaoxia Long, Yan Wang, Penglu Yan, Xiaoli Wei, authorsList=Chaoxia Long, Yan Wang, Penglu Yan, Xiaoli Wei, authorCompany=null, correspAuthors=Xiaoli Wei, authorNote=null, correspAuthorsNote=null, copyrightStatement=© 2026 Journal of Central South University of Forestry & Technology, copyrightOwner=Journal of Central South University of Forestry & Technology, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322924406699336652, articleId=1322924404279223240, tenantId=1146029695717560320, journalId=1322258771877134531, language=CN, title=珍稀濒危树种小叶红豆离体快繁技术体系的构建, columnId=1322924272984929210, journalTitle=中南林业科技大学学报, columnName=生物学, runingTitle=null, highlight=null, articleAbstract=
【目的】 小叶红豆Ormosia microphylla是我国特有的珍稀濒危树种，属于国家一级保护野生植物，具有极高的经济与生态价值。由于种子来源有限，传统的种子育苗方法繁殖系数低，因而组织培养技术成为小叶红豆快速繁殖的重要途径，本研究旨在探讨小叶红豆的组培快繁体系。
【方法】 以小叶红豆成熟种子为材料进行无菌苗培养试验，筛选出最佳种子消毒方案；以萌发的无菌苗带芽茎段为外植体，采用L9（33）正交试验设计筛选出最佳诱导与增殖培养基；在继代培养阶段，分析了不同继代次数对增殖效果的影响；最后，通过生根及移栽试验，筛选出最佳的生根培养基和最适移栽基质。
【结果】 无菌苗培养的最佳种子消毒方案为保留种皮并用2% NaClO溶液消毒12 min，该条件下未出现污染，无菌苗获得率为70.37%，褐化率为23.23%。最佳增殖培养基为MS+1.5 mg·L-1 6-BA+0.15 mg·L-1 IAA，增殖系数为5.23，平均芽苗高为3.59 cm。继代培养中，第3次继代的增殖效果最佳，增殖系数为5.33。最佳生根培养基为1/2 MS + 0.3 mg·L-1 NAA，生根率93.33%，平均生根数3.95条，平均根长3.63 cm。以蛭石∶珍珠岩（1∶1）为基质移栽成活率最高，为95.56%。
【结论】 本研究建立了一套较稳定高效的小叶红豆离体快繁技术体系，通过对外植体灭菌、芽诱导与增殖、继代培养和生根移栽等关键环节的试验研究，为小叶红豆种质资源保护与规模化繁殖提供理论依据与技术支撑。
, authors=龙超霞, 王言, 颜鹏露, 韦小丽, authorsList=龙超霞, 王言, 颜鹏露, 韦小丽, authorCompany=null, correspAuthors=韦小丽, authorNote=
, correspAuthorsNote=
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龙超霞（18585886082@163.com），硕士研究生。
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龙超霞（18585886082@163.com），硕士研究生。
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