中南林业科技大学学报

根萌复幼对刺槐扦插苗叶片形态、养分及光合生理的影响

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朱凯轩 ¹ , 张子涵 ¹ , 彭祚登 ¹ , 温静 ² , 许沐阳 ³ , 冯雨晗嫣 ¹ , 赵任义 ²

中南林业科技大学学报 | 林学 2026,46(10): 102-113

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中南林业科技大学学报 |林学 2026 , 46 (10) : 102 -113

根萌复幼对刺槐扦插苗叶片形态、养分及光合生理的影响

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朱凯轩¹, 张子涵¹, 彭祚登¹, 温静², 许沐阳³, 冯雨晗嫣¹, 赵任义²

作者信息

^1.北京林业大学　林木资源高效生产全国重点实验室，北京　100083

^2.北京市西山试验林场管理处，北京　100093

^3.干旱半干旱地区森林培育及生态系统研究国家林草局重点实验室，北京　100083

通讯作者:

彭祚登（zuodengpeng@sina.com），教授，博士，博士研究生导师。

作者简介:

朱凯轩（zkx20010612@163.com），硕士研究生。

Effects of rejuvenation of root-sprout on leaf morphology, nutrients and photosynthetic physiology of Robinia pseudoacacia cuttings

Kaixuan Zhu¹, Zihan Zhang¹, Zuodeng Peng¹, Jing Wen², Muyang Xu³, Yuhanyan Feng¹, Renyi Zhao²

Affiliations

^1.State Key Laboratory of Efficient Production of Forest Resources, Beijing Forestry University, Beijing 100083, China

^2.Beijing Xishan Experimental Forest Farm, Beijing 100093, China

^3.Key Laboratory for Silviculture and Forest Ecosystem Research in Arid and Semi-arid Region of State Forestry and Grassland Administration, Beijing 100083, China

出版时间: 2026-10-25 doi: 10.14067/j.cnki.1673-923x.2026.10.009

文章导航

摘要

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【目的】

探究根萌复幼措施对刺槐扦插苗叶片生长与生理的影响，为今后生产实践中刺槐复幼体系的建立提供理论依据。

【方法】

以刺槐根萌条和母树半木质化枝条为繁殖材料进行扦插育苗，设置刺槐1年生播种苗为对照，比较不同繁殖材料所培育苗木的叶片形态与解剖结构、干质量、鲜质量、气体交换参数、叶绿素荧光参数、光合酶活性及养分生理的差异。

【结果】

与播种苗和母树枝条扦插苗相比，根萌复幼处理显著提高了刺槐扦插苗叶片的叶长、叶宽、叶周长、叶面积、干质量、鲜质量和叶轴长等，显著降低了叶片上表皮厚度、栅栏组织厚度、叶片厚度；复幼后的苗木与播种苗相比，海绵组织厚度、叶片紧密度、叶片疏松度、栅海比无显著变化；根萌复幼处理显著提高了刺槐苗光合色素含量、净光合速率、蒸腾速率、F_v/F_m、F_v/F_o、NPQ和叶绿素荧光参数，其中一年生根萌条最高；根萌复幼后苗木的果糖1,6-二磷酸醛缩酶（FBA）、核酮糖-1,5-二磷酸羧化酶/加氧酶（Rubisco）活性较母树枝条扦插苗有显著提高，但与播种苗仍存在显著差异；根萌复幼处理提高了叶片C含量，降低了叶片N、P含量，但C/N、N/P、NSC和淀粉含量及叶片干、鲜质量增加，经根萌复幼后的苗木具有更高的养分利用效率和干物质积累量，其中一年生根萌条最高。

【结论】

经根萌复幼的刺槐扦插苗具有更强的生长潜力与幼化性，这为刺槐的良种繁育和壮苗培育提供了理论支持。

关键词

刺槐 / 根萌复幼 / 光合生理 / 无性繁殖

Abstract

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【Objective】

The aim of this experiment was to explore the effects of root sprouting rejuvenation on the growth of Robinia pseudoacacia cutting seedlings, and provide a theoretical basis for the establishment of Robinia pseudoacacia rejuvenation system in the future production practice.

【Method】

The cuttings of Robinia pseudoacacia root sprouts and semi-lignified branches of the mother tree were used as propagation materials, and the 1-year-old seedlings of Robinia pseudoacacia were set as the control. The differences in leaf morphology and anatomical structure, dry and fresh weight, gas exchange parameters, chlorophyll fluorescence parameters, photosynthetic enzyme activity and nutrient physiology of seedlings cultivated with different propagation materials were determined.

【Result】

The results showed that, compared with the seedlings and mother tree branch cuttings, the root sprouting and rejuvenation treatment significantly increased the leaf length, leaf width, leaf perimeter, leaf area, dry and fresh weight, and leaf axis length of Robinia pseudoacacia cuttings, and significantly reduced the thickness of the upper epidermis, palisade tissue, and leaf thickness. Compared with seeded seedlings, the thickness of spongy tissue, leaf compactness, leaf porosity and the P/S ratio had no significant changes. The content of photosynthetic pigment, net photosynthetic rate, transpiration rate, F_v/F_m, F_v/F_o, NPQ and other gas exchange parameters, chlorophyll fluorescence parameters of Robinia pseudoacacia seedlings were significantly increased by root sprouting and rejuvenation treatment, and the rooting sprouts in one year were the highest. The activities of fructose 1,6-bisphosphate aldolase (FBA) and ribulose-1,5-diphosphate carboxylase/oxygenase (Rubisco) in the seedlings after root sprouting and rejuvenation were significantly higher than those in the mother branch cuttings, but there were still significant differences between the seedlings and the seedlings; However, the C/N, N/P, NSC and starch contents as well as the dry and fresh weight of leaves increased. The seedlings after root sprouting and rejuvenation had higher nutrient utilization efficiency and dry matter accumulation, and the one-year-old rooting shoots was the highest.

【Conclusion】

The cuttings of Robinia pseudoacacia, which have been rejuvenated by root sprouting, have stronger growth potential and juvenility, which provides theoretical support for the breeding of improved varieties and strong seedlings of Robinia pseudoacacia.

Key words

Robinia pseudoacacia / rejuvenation of root-sprout / photosynthetic physiology / clonal propagation

引用本文

朱凯轩, 张子涵, 彭祚登, 温静, 许沐阳, 冯雨晗嫣, 赵任义. 根萌复幼对刺槐扦插苗叶片形态、养分及光合生理的影响. 中南林业科技大学学报, 2026 , 46 (10) : 102 -113 . DOI: 10.14067/j.cnki.1673-923x.2026.10.009

Kaixuan Zhu, Zihan Zhang, Zuodeng Peng, Jing Wen, Muyang Xu, Yuhanyan Feng, Renyi Zhao. Effects of rejuvenation of root-sprout on leaf morphology, nutrients and photosynthetic physiology of Robinia pseudoacacia cuttings[J]. Journal of Central South University of Forestry & Technology, 2026 , 46 (10) : 102 -113 . DOI: 10.14067/j.cnki.1673-923x.2026.10.009

基金

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“十四五”国家重点研发计划项目课题(2024YFD2200205)

参考文献

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文献

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参考文献引证文献

排序方式：

[1]

康向阳. 关于无性系林业若干问题的认识和建议—以杨树为例[J]. 北京林业大学学报, 2017, 39(9): 1-7.

Kang

X Y

. Cognition and suggestions on some issues related to clonal forestry: taking poplar as an example[J]. Journal of Beijing Forestry University, 2017, 39(9): 1-7.

[2]

Prescott

C E

, Grayston

S J

, Helmisaari

H S

, et al. Surplus carbon drives allocation and plant-soil interactions[J]. Trends in Ecology & Evolution, 2020, 35(12): 1110-1118.

[3]

康向阳. 新一轮毛白杨遗传改良策略的思考和实践[J]. 北京林业大学学报, 2016, 38(7): 1-8.

Kang

X Y

. Thinking and practices for strategy on a new round genetic improvement of Populus tomentosa[J]. Journal of Beijing Forestry University, 2016, 38(7): 1-8.

[4]

朱之悌. 树木的无性繁殖与无性系育种[J]. 林业科学, 1986, 22(3): 280-290.

Zhu

Z T

. Vegetative propagation and clonal breeding of forest trees[J]. Scientia Silvae Sinicae, 1986, 22(3): 280-290.

[5]

杨舜垚. 湿加松平茬复幼机理研究[D]. 北京: 北京林业大学, 2022: 3-4.

Yang

S Y

. The mechanism of Pinus elliottii×P. caribaea rejuvenation after stem pruning[D]. Beijing: Beijing Forestry University, 2022: 3-4.

[6]

彭方仁. 无性繁殖的进展及其在无性系林业中应用前景[J]. 世界林业研究, 1990, 3(4): 74-81.

Peng

F R

. Development of asexual reproduction and its application prospect in clonal forestry[J]. World Forestry Research, 1990, 3(4): 74-81.

[7]

刘杰, 孙宇涵, 袁存权, 等. 林木根萌复幼方式的研究进展[J]. 分子植物育种, 2022, 20(14): 4867-4872.

Liu

, Sun

Y H

, Yuan

C Q

, et al. A review for rejuvenation of root-sprout in trees[J]. Molecular Plant Breeding, 2022, 20(14): 4867-4872.

[8]

裴东, 谷瑞升. 树木复幼的研究概述[J]. 植物学通报, 2005, 22(6): 753-760.

Pei

, Gu

R S

. A review on the rejuvenation of mature trees[J]. Chinese Bulletin of Botany, 2005, 22(6): 753-760.

[9]

Zhang

Z J

, Sun

Y H

, Li

. Plant rejuvenation: from phenotypes to mechanisms[J]. Plant Cell Reports, 2020, 39: 1249-1262.

[10]

, Chen

T T

, Khan

W U

, et al. Regulatory roles of miRNAs associated with the aging pathway in tree vegetative phase changes[J]. Forestry Research, 2023, 3: 9.

[11]

张长庆. 黄土高原丘陵沟壑区刺槐人工林和狼牙刺天然林生殖与更新研究[D]. 杨凌: 西北农林科技大学, 2008: 4.

Zhang

C Q

. Contrast study on the reproduction and regeneration of Robinia pseudoacacia plantation and Sophora davidii natural community in hilly-gully region of the Loess Plateau[D]. Yangling: Northwest A & F University, 2008: 4.

[12]

史戈. 基于SSR分子标记技术的刺槐萌蘖扩散格局的研究[D]. 泰安: 山东农业大学, 2020: 9-10.

Shi

. The dispersal pattern of sprouting regeneration of Robinia pseudoacacia based on SSR molecular marker technology[D]. Tai'an: Shandong Agricultural University, 2020: 9-10.

[13]

姜雅轩, 孔德馨, 雷培, 等. 生物菌肥对四倍体刺槐植株生长的影响[J]. 中南林业科技大学学报, 2023, 43(8): 27-35.

Jiang

Y X

, Kong

D X

, Lei

, et al. Effects of biological fungus fertilizers on the growth of tetraploid Robinia pseudoacacia L.[J]. Journal of Central South University of Forestry & Technology, 2023, 43(8): 27-35.

[14]

张子杰. 刺槐根繁幼化的生理差异与分子机制研究[D]. 北京: 北京林业大学, 2022: 2-12.

Zhang

Z J

. Physiological differences and molecular mechanisms of rejuvenation during root-based vegetative propagation in Robinia pseudoacacia[D]. Beijing: Beijing Forestry University, 2022: 2-12.

[15]

耿兵, 王华田, 王延平, 等. 刺槐萌生林与实生林的生长比较[J]. 中国水土保持科学, 2013, 11(2): 59-64.

Geng

, Wang

H T

, Wang

Y P

, et al. Comparative study of coppice and seeding forest of Robinia pseudoacacia L.[J]. Science of Soil and Water Conservation, 2013, 11(2): 59-64.

[16]

李合生, 孙群, 赵世杰, 等. 植物生理生化实验原理和技术[M] 北京: 高等教育出版社, 2000: 134-137.

H S

, Sun

, Zhao

S J

, et al. Principles and techniques of plant physiological biochemical experiment[M]. Beijing: Higher Education Press, 2000: 134-137.

[17]

王学奎, 黄见良. 植物生理生化实验原理和技术[M]. 3版. 北京: 高等教育出版社, 2015: 138-140.

Wang

X K

, Huang

J L

. Principles and techniques of plant physiological and biochemical experiments[M]. 3rd ed. Beijing: Higher Education Press, 2015: 138-140.

[18]

张星. 刺槐萌生林化学计量特征及其土壤细菌多样性研究[D]. 泰安: 山东农业大学, 2019: 13-14.

Zhang

. Stoichiometric characteristics and soil microbial diversity of Robinia pseudoacacia sprout forests[D]. Taian: Shandong Agricultural University, 2019: 13-14.

[19]

常云妮. 采穗圃营建方式对杉木繁殖体幼化的影响机制[D]. 福州: 福建师范大学, 2023: 92-95.

Chang

Y N

. The influencing mechanism of different establishment methods in cutting orchard on rejuvenation for vegetative propagation of Cunninghamia lanceolata[D]. Fuzhou: Fujian Normal University, 2023: 92-95.

[20]

张舒乐, 彭祚登, 李香. 土壤水分条件对刺槐实生苗叶片功能性状及生长潜力的影响[J]. 安徽农业大学学报, 2025, 52(3): 386-396.

Zhang

S L

, Peng

Z D

, Li

. Effects of soil moisture conditions on the leaf functional traits and the growth potential of Robinia pseudoacacia seedlings[J]. Journal of Anhui Agricultural University, 2025, 52(3): 386-396.

[21]

Zhang

, Zhang

L L

, Chai

Y Y

, et al. Physiology and proteomics research on the leaves of ancient Platycladus orientalis (L.) during winter[J]. Journal of Proteomics, 2015, 126: 263-278.

[22]

Pijut

P M

, Wowste

K E

, Michler

C H

. Promotion of adventitious root formation of difficult-to-root hardwood tree species[J]. Horticultural Reviews, 2011, 38: 213-251.

[23]

刘全宏, 王孝安, 田先华, 等. 太白红杉(Larix chinensis)叶的形态解剖学特征与环境因子的关系[J]. 西北植物学报, 2001, 21(5): 885-893.

Liu

Q H

, Wang

X A

, Tian

X H

, et al. Morphological and anatomical characteristics of leaf of Larix chinensis and their relationship to environmental factors in Taibaishan mountain[J]. Acta Botanica Boreali-Occidentalia Sinica, 2001, 21(5): 885-893.

[24]

Arnaud

, Franclet

, Tranvan

, et al. Micropropagation and rejuvenation of Sequoia sempervirens (Lamb) Endl: a review[J]. Annales des Sciences Forestières, 1993, 50: 273-295.

[25]

韩文哲, 刘焕臻, 王兆宁, 等. 复幼对五角槭生长､解剖结构和生理指标的影响[J]. 西北林学院学报, 2025, 40(4): 157-165.

Han

W Z

, Liu

H Z

, Wang

Z N

, et al. Effects of rejuvenation on growth, anatomy and physiological indices of Acer mono[J]. Journal of Northwest Forestry University, 2025, 40(4): 157-165.

[26]

Hussain

, Shah

, Ali

, et al. Role of nitric oxide in plant senescence[J]. Frontiers in Plant Science, 2022, 13: 851631.

[27]

张劲. 雄性毛白杨古树良种选育及老化机理研究[D]. 北京: 北京林业大学, 2023: 6.

Zhang

. Studies on the selecting and breeding of improved varieties and aging mechanism of the old male Populus tomentosa[D]. Beijing: Beijing Forestry University, 2023: 6.

[28]

宋慧, 冯佰利, 高小丽, 等. 不同小豆品种(系)叶片衰老与活性氧代谢[J]. 作物学报, 2010, 36(2): 347-353.

Song

, Feng

B L

, Gao

X L

, et al. Leaf senescence and reactive oxygen metabolism in different adzuki bean cultivars (lines)[J]. Acta Agronomica Sinica, 2010, 36(2): 347-353.

[29]

Basheer-Salimia

, Patakas

, Noitsakis

, et al. Changes of morphological and physiological markers induced by growth phases in leaves of olive tree (Olea europaea L.)[J]. Journal of Biological Research, 2004, 2: 105-114.

[30]

洪汉辉. 插穗年龄对白杨生根过程生化特征及新生苗生长的影响[D]. 北京: 北京林业大学, 2018: 41-42.

Hong

H H

. Effect of cutting age on the biochemical feature of rooting process and the growth of new-born seedlings in populus[D]. Beijing: Beijing Forestry University, 2018: 41-42.

[31]

刘欢欢, 王艳, 陈婉莹, 等. 玉米穗位叶快速叶绿素荧光参数及产量相关性状QTL定位[J]. 分子植物育种, 2022, 20(6): 1872-1882.

Liu

H H

, Wang

, Chen

W Y

, et al. QTL mapping for fast chlorophyll fluorescence parameters of ear leaves and yield-related traits in maize[J]. Molecular Plant Breeding, 2022, 20(6): 1872-1882.

[32]

Demmig-Adams

, Koh

S C

, Cohu

C M

, et al. Non-photochemical fluorescence quenching in contrasting plant species and environments[M]//Non-photochemical quenching and energy dissipation in plants, algae and cyanobacteria, Dordrecht: Springer, 2014: 531-552.

[33]

王鑫喆, 彭祚登, 李一鸣. 施磷肥对刺槐萌生林生长与营养积累的影响[J]. 中南林业科技大学学报, 2025, 45(8): 95-102.

Wang

X Z

, Peng

Z D

, Li

Y M

. Effects of phosphorus fertilization on the growth and nutrient accumulation of Robinia pseudoacacia germination forests[J]. Journal of Central South University of Forestry & Technology, 2025, 45(8): 95-102.

[34]

Townsend

A R

, Cleveland

C C

, Asner

G P

, et al. Controls over foliar N∶P ratios in tropical rain forests[J]. Ecology, 2007, 88(1): 107-118.

[35]

孙华. 土壤质量对植物光合生理生态功能的影响研究进展[J]. 中国生态农业学报, 2005, 13(1): 122-124.

Sun

. Influence of soil qualities on the photosynthesis, physiological and ecological functions of vegetable[J]. Chinese Journal of Eco-Agriculture, 2005, 13(1): 122-124.

[36]

叶洲辰, 杨顺, 于添化, 等. 复幼处理对小黑杨叶片营养成分及光合特性的影响[J]. 森林工程, 2025, 41(6): 1145-1155.

Z C

, Yang

, Yu

T H

, et al. Effects of rejuvenation treatment on nutritional composition and photosynthetic characteristics of Populus simonii×P. nigra leaves[J]. Forest Engineering, 2025, 41(6): 1145-1155.

[37]

张富森. 根瘤菌和丛枝菌根真菌与刺槐共生对镉及低氮胁迫的响应机制研究[D]. 重庆: 西南大学, 2023: 12-13.

Zhang

F S

. Responses of rhizobia and arbuscular mycorrhizal fungi to cadmium and nitrogen stress in Robinia pseudoacaci[D]. Chongqing: Southwest University, 2023: 12-13.

[38]

Sardans

, Penuelas

. The role of plants in the effects of global change on nutrient availability and stoichiometry in the plant-soil system[J]. Plant Physiology, 2012, 160: 1741-1761.

2026年第46卷第10期

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doi: 10.14067/j.cnki.1673-923x.2026.10.009

接收时间：2025-09-23
首发时间：2026-10-30
出版时间：2026-10-25

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出版历史

收稿日期：2025-09-23
修回日期：2025-12-26

基金

“十四五”国家重点研发计划项目课题(2024YFD2200205)

作者信息

^1.北京林业大学　林木资源高效生产全国重点实验室，北京　100083

^2.北京市西山试验林场管理处，北京　100093

^3.干旱半干旱地区森林培育及生态系统研究国家林草局重点实验室，北京　100083

通讯作者:

彭祚登（zuodengpeng@sina.com），教授，博士，博士研究生导师。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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