中南林业科技大学学报

直干桉高效组培快繁体系构建及高繁殖能力基因型筛选

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周玉吉 ¹ , 曹越 ² , 王鸿嶙 ¹ , 孔令荣 ¹ , 吕少念 ¹ , 侯玉帆 ¹ , 陆庄跃 ¹ , 何真敏 ¹ , 曹子林 ¹ , 王晓丽 ¹

中南林业科技大学学报 | 生物学 2026,46(8): 205-215

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中南林业科技大学学报 |生物学 2026 , 46 (8) : 205 -215

直干桉高效组培快繁体系构建及高繁殖能力基因型筛选

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周玉吉¹, 曹越², 王鸿嶙¹, 孔令荣¹, 吕少念¹, 侯玉帆¹, 陆庄跃¹, 何真敏¹, 曹子林¹, 王晓丽¹

作者信息

^1.西南林业大学　a. 西南山地森林资源保育与利用教育部重点实验室；b. 生态与水土保持学院，云南　昆明　650224

^2.湖北省林业科学研究院，湖北　武汉　430075

通讯作者:

王晓丽（ynwangxiaoli@swfu.edu.cn），教授，博士，博士研究生导师。

作者简介:

周玉吉（2997202147@qq.com），硕士研究生。

Construction of a highly efficient tissue culture and rapid propagation system for Eucalyptus maidenii and screening of genotypes with high reproductive capacity

Yuji Zhou¹, Yue Cao², Honglin Wang¹, Lingrong Kong¹, Shaonian Lyu¹, Yufan Hou¹, Zhuangyue Lu¹, Zhenmin He¹, Zilin Cao¹, Xiaoli Wang¹

Affiliations

^1.a. Key Laboratory of Forest Resources Conservation and Utilization in the Southwest Mountains, Ministry of Education; b. College of Ecology and Soil and Water Conservation, Southwest Forestry University, Kunming 650224, Yunnan, China

^2.Hubei Academy of Forestry, Wuhan 430075, Hubei, China

出版时间: 2026-08-25 doi: 10.14067/j.cnki.1673-923x.2026.08.020

文章导航

摘要

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【目的】

构建直干桉Eucalyptus maidenii组培快繁体系，筛选具有高繁殖能力的基因型，为直干桉工厂化育苗提供重要的理论基础和技术支持。

【方法】

以直干桉优良家系的种子为试验材料，采用全因子和正交试验等方法，探究直干桉初代培养、继代增殖、不定根诱导和炼苗移栽的最适培养条件，并进一步利用最适增殖和生根培养基对直干桉不同基因型无菌苗进行培养，从而筛选具备高增殖能力和强生根能力的基因型。

【结果】

直干桉无菌苗茎段初代培养和继代增殖的最适培养条件均为MS培养基+0.5 mg/L 6-BA+0.6 mg/L NAA+20 g/L蔗糖+4.5 g/L琼脂，腋芽诱导率可达100%，月增殖倍数为10.40倍，苗高为1.14 cm，丛芽生长健壮；不定根诱导的最适培养条件为1/2 DCR培养基+0.6 mg/L IBA+0.3 mg/L NAA+1.0 mg/L ABT1+20 g/L蔗糖+5 g/L琼脂+0.16 g/L活性炭，培养20 d时生根率为86.67%；移栽最适基质配比为腐殖质土∶蛭石∶泥炭土=1∶1∶1，成活率可达76.67%。对直干桉118个基因型进行增殖和生根培养，最终筛选出1个具备高增殖能力和强生根能力的基因型（G116），其培养60 d后增殖倍数为11.76倍，苗高为1.65 cm，丛芽生长健壮，培养20 d后生根率可达93.33%。

【结论】

成功构建了直干桉高效组培快繁体系，筛选出了1个具有高繁殖能力的基因型，提高了育苗效率。

关键词

直干桉 / 无菌实生瓶苗 / 组培快繁 / 基因型筛选

Abstract

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【Objective】

This study aims to develop an efficient tissue culture propagation system for Eucalyptus maidenii and identify high- performance genotypes, thereby establishing a theoretical and technical foundation for large-scale seedling production.

【Method】

Seeds from elite E. maidenii families were used as explants. Using full factorial and orthogonal experimental designs, the optimal culture conditions were determined for primary culture, subculture proliferation, adventitious root induction, and hardening. The selected optimal proliferation and rooting media were then applied to axenic seedlings of different genotypes to screen genotypes that exhibited both high proliferation efficiency and robust rooting competence.

【Result】

The optimal conditions for the primary culture and subculture of sterile stem segments of E. maidenii are MS medium supplemented with 0.5 mg/L 6-BA, 0.6 mg/L NAA, 20 g/L sucrose, and 4.5 g/L agar, resulting in a 100% axillary bud induction rate, a 10.40-fold monthly proliferation rate, a plantlet height of 1.14 cm, and robust shoot clusters. Adventitious root induction is optimally achieved on 1/2 DCR medium containing 0.6 mg/L IBA, 0.3 mg/L NAA, 1.0 mg/L ABT1, 20 g/L sucrose, 5 g/L agar, and 0.16 g/L activated charcoal, with a 86.67% rooting rate after 20 days. A 1:1:1 (v/v/v) mixture of humus soil, vermiculite, and peat soil provides the highest transplantation success, with a 76.67% survival rate. A total of 118 E. maidenii genotypes were evaluated for proliferation and rooting capacity, from which one superior genotype (G116) with both high proliferation efficiency and strong rooting ability was selected. After 60 days of culture, G116 exhibits a 11.76-fold proliferation rate, reaches 1.65 cm in height, and develops robust shoot clusters. This genotype also demonstrates a 93.33% rooting rate within 20 days of culture.

【Conclusion】

The research successfully developed an efficient in vitro propagation system for E. maidenii and selected one genotype exhibiting superior propagation traits. This methodology enhanced seedling production efficiency.

Key words

Eucalyptus maidenii / aseptic seedling bottle culture / tissue culture rapid propagation / genotype screening

引用本文

周玉吉, 曹越, 王鸿嶙, 孔令荣, 吕少念, 侯玉帆, 陆庄跃, 何真敏, 曹子林, 王晓丽. 直干桉高效组培快繁体系构建及高繁殖能力基因型筛选. 中南林业科技大学学报, 2026 , 46 (8) : 205 -215 . DOI: 10.14067/j.cnki.1673-923x.2026.08.020

Yuji Zhou, Yue Cao, Honglin Wang, Lingrong Kong, Shaonian Lyu, Yufan Hou, Zhuangyue Lu, Zhenmin He, Zilin Cao, Xiaoli Wang. Construction of a highly efficient tissue culture and rapid propagation system for Eucalyptus maidenii and screening of genotypes with high reproductive capacity[J]. Journal of Central South University of Forestry & Technology, 2026 , 46 (8) : 205 -215 . DOI: 10.14067/j.cnki.1673-923x.2026.08.020

基金

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国家自然科学基金项目(32260385)
云南省种子种业联合实验室项目(202205AR070001-22)
云南省高校服务重点产业科技专项(FWCY-ZNT2024022)
西南林业大学科研启动基金项目(112005)

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2026年第46卷第8期

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文章信息

doi: 10.14067/j.cnki.1673-923x.2026.08.020

接收时间：2025-07-23
首发时间：2026-10-30
出版时间：2026-08-25

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出版历史

收稿日期：2025-07-23
修回日期：2025-09-16

基金

国家自然科学基金项目(32260385)

云南省种子种业联合实验室项目(202205AR070001-22)

云南省高校服务重点产业科技专项(FWCY-ZNT2024022)

西南林业大学科研启动基金项目(112005)

作者信息

^1.西南林业大学　a. 西南山地森林资源保育与利用教育部重点实验室；b. 生态与水土保持学院，云南　昆明　650224

^2.湖北省林业科学研究院，湖北　武汉　430075

通讯作者:

王晓丽（ynwangxiaoli@swfu.edu.cn），教授，博士，博士研究生导师。

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https://castjournals.cast.org.cn/joweb/znlykj/CN/10.14067/j.cnki.1673-923x.2026.08.020

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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