中南林业科技大学学报

福建柏不定芽诱导及内源激素研究

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卢冰冰 ^a , 肖帅 ^a , 王雨溪 ^b , 肖集泓 ^b , 王小妹 ^a , 荣俊冬 ^a , 陈礼光 ^a , 郑郁善 ^a^,^b

中南林业科技大学学报 | 林学 2026,46(8): 46-55

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中南林业科技大学学报 |林学 2026 , 46 (8) : 46 -55

福建柏不定芽诱导及内源激素研究

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卢冰冰^a, 肖帅^a, 王雨溪^b, 肖集泓^b, 王小妹^a, 荣俊冬^a, 陈礼光^a, 郑郁善^a^,^b

作者信息

^a.福建农林大学　林学院，福建　福州　350000

^b.福建农林大学　风景园林与艺术学院，福建　福州　350000

通讯作者:

陈礼光（fjclg@qq.com），副教授，博士，硕士研究生导师。

作者简介:

卢冰冰（13183090276@163.com），硕士。

Induction of adventitious buds and study on endogenous hormones in Chamaecyparis hodginsii

Bingbing Lu^a, Shuai Xiao^a, Yuxi Wang^b, Jihong Xiao^b, Xiaomei Wang^a, Jundong Rong^a, Liguang Chen^a, Yushan Zheng^a^,^b

Affiliations

^a.College of Forestry, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, China

^b.School of Landscape Architecture and Art, Fujian Agriculture and Forestry University, Fuzhou 350000, Fujian, China

出版时间: 2026-08-25 doi: 10.14067/j.cnki.1673-923x.2026.08.005

文章导航

摘要

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【目的】

聚焦福建柏Chamaecyparis hodginsii不定芽诱导体系的优化，为其种质资源保护与组培离体繁殖提供理论支撑。

【方法】

通过筛选6-BA、ZT、KT等细胞分裂素，明确最适宜的外植体、外植体消毒配方以及不定芽诱导配方，探究芽诱导培养内源激素机理。

【结果】

1）福建柏最适宜的外植体为当年生带芽茎段；2）75%乙醇消毒10 s，4%次氯酸钠消毒600 s组合处理为外植体最佳消毒配方，能显著降低污染风险；3）福建柏不定芽诱导的最佳培养基配方为MS+Sucrose 30.0 g/L+Agar 6.5 g/L+KT 0.1 mg/L，芽诱导率达85.56%。AC和VC组合处理的抗褐化效果显著，污染率低于10%，有效提升了培养效率；4）不定芽诱导过程中的内源激素动态变化表明，IAA、ABA、GA₃、CTK含量及相互间比值呈现规律性波动。其中，ABA与GA₃存在拮抗作用，而IAA/ABA和CTK/IAA的平衡是调控福建柏芽萌发的核心机制；培养7～14 d，IAA含量、IAA/ABA和CTK/IAA达峰值，这一时期是福建柏芽萌发的关键阶段，此时激素的协同作用共同构成了其适应环境、促进生长发育的调控机制。

【结论】

初步建立了福建柏低污染不定芽诱导体系，明确了不同培养条件对内源激素含量的影响，揭示了内源激素在不定芽诱导过程中的调控规律。

关键词

福建柏 / 不定芽 / 抗褐化剂 / 内源激素

Abstract

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【Objective】

Focusing on the optimization of the adventitious bud induction system of Chamaecyparis hodginsii, a native species of Fujian, this study aims to provide theoretical support for the conservation of its germplasm resources and the development of in vitro propagation techniques.

【Method】

By screening 6-BA, ZT, KT and other cytokinins, determining the most suitable implants, the best sterilization formula for implants, and the best incubation formula for adventitious bud induction, and exploring the mechanism of endogenous hormones in germination.

【Result】

1) C. hodginsii is most suitable for extraction as a young, young, sprouted stem that was born in the year; 2) 75% alcohol disinfection for 10 s, 4% sodium hypochlorite disinfection for 600 s was the best disinfection formula of explants, which significantly reduced the risk of contamination; 3) The optimum culture medium for adventitious bud induction was MS + Sucrose 30.0 g/L + Agar 6.5 g/L + 0.1 mg/L KT, the rate of bud induction was 85.56%. At the same time, for the common browning problem in tissue culture of C. hodginsii, we found that the combination of AC and VC treatment had significant anti-browning effect, and the pollution rate was below 10%, effectively improving the culture efficiency; 4) The dynamic changes in the endogenous hormone levels during the process of adventitious bud induction indicate that the concentrations and ratios of IAA, ABA, GA₃, and CTK exhibit regular fluctuations. Among these, ABA and GA₃ have antagonistic effects. The balance between the ratios of IAA/ABA and CTK/IAA is the core mechanism regulating the bud germination of C. hodginsii. The period of 7-14 days is when the concentrations of IAA and the ratios of IAA/ABA, CTK/IAA reach their peak levels, making it a critical stage for the bud germination of C. hodginsii. During this period, the synergistic action of these hormones collectively contributes to their ability to adapt to the environment and promote growth and development.

【Conclusion】

This study has preliminarily established a low-pollution adventitious bud induction system for C. hodginsii, clarified the impact of different cultivation conditions on the content of endogenous hormones, and revealed the regulatory rules of endogenous hormones in the process of adventitious bud induction.

Key words

Chamaecyparis hodginsii / adventitious buds / anti-browning agent / endogenous hormones

引用本文

卢冰冰, 肖帅, 王雨溪, 肖集泓, 王小妹, 荣俊冬, 陈礼光, 郑郁善. 福建柏不定芽诱导及内源激素研究. 中南林业科技大学学报, 2026 , 46 (8) : 46 -55 . DOI: 10.14067/j.cnki.1673-923x.2026.08.005

Bingbing Lu, Shuai Xiao, Yuxi Wang, Jihong Xiao, Xiaomei Wang, Jundong Rong, Liguang Chen, Yushan Zheng. Induction of adventitious buds and study on endogenous hormones in Chamaecyparis hodginsii[J]. Journal of Central South University of Forestry & Technology, 2026 , 46 (8) : 46 -55 . DOI: 10.14067/j.cnki.1673-923x.2026.08.005

基金

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福建省林业种苗科技攻关项目(ZMGG-0811)
福建农林大学科技创新专项(KFB23057)

参考文献

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文献

收起

参考文献引证文献

排序方式：

[1]

刘凯, 韩永振, 王希贤, 等. 不同种源福建柏生长性状的差异与评价[J]. 中南林业科技大学学报, 2023, 43(3): 62-72.

Liu

, Han

Y Z

, Wang

X X

, et al. Difference and evaluation of grow traits of Fokienia hodginsii caused by different provenances[J]. Journal of Central South University of Forestry & Technology, 2023, 43(3): 62-72.

[2]

张文亮, 张燕, 边时羽, 等. 百合组织培养技术概论[J]. 河北农业, 2025(5): 104-106.

Zhang

W L

, Zhang

, Bian

S Y

, et al. Introduction to tissue culture technology of lily[J]. Hebei Agriculture, 2025(5): 104-106.

[3]

汪长水. 福建柏茎段腋芽继代培养技术研究[J]. 林业勘察设计, 2010, 30(1): 84-87.

Wang

C S

. Axillary buds subculture technology of Fujian cypress[J]. Forestry Prospect and Design, 2010, 30(1): 84-87.

[4]

常二梅, 江泽平, 刘建锋, 等. 侧柏古树组织培养体系的建立[J]. 林业科学研究, 2023, 36(4): 41-49.

Chang

E M

, Jiang

Z P

, Liu

J F

, et al. Establishment of tissue culture system of the ancient trees of Platycladus orientalis[J]. Forest Research, 2023, 36(4): 41-49.

[5]

王小妹, 吴霞, 张禹欣, 等. 福建柏愈伤组织诱导与抗褐化研究[J]. 西北农林科技大学学报(自然科学版), 2024, 52(5): 57-68.

Wang

X M

, Wu

, Zhang

Y X

, et al. Healing tissue induction and anti-browning of Fokienia hodginsii[J]. Journal of Northwest A & F University (Natural Science Edition), 2024, 52(5): 57-68.

[6]

张秀华. 龙柏组织培养研究[J]. 安徽农业科学, 2011, 39(24): 14548-14549, 14592.

Zhang

X H

. Study on the tissue culture of Sabina chinensis cv. Kaizuka[J]. Journal of Anhui Agricultural Sciences, 2011, 39(24): 14548-14549, 14592.

[7]

赵育新. 侧柏古树组织培养再生及其端粒相关基因克隆研究[D]. 北京: 中国林业科学研究院, 2015: 44.

Zhao

Y X

. Researches on tissue culture of old Platycladus orientalis and cloning relevant genes of telomere[D]. Beijing: Chinese Academy of Forestry, 2015: 44.

[8]

赵阳, 何炎红, 林涛, 等. 胭脂红杏果实发育过程中内源激素与糖酸代谢的关联研究[J]. 果树学报, 2025, 42(9): 2080-2096.

Zhao

, He

Y H

, Lin

, et al. Association study between endogenous hormones and sugar-acid metabolism during fruit development in Yanzhihong apricot[J]. Journal of Fruit Science, 2025, 42(9): 2080-2096.

[9]

Fang

C W

, Chen

H Y

, Castillo-Díaz

, et al. Regeneration and endogenous phytohormone responses to high-temperature stress drive recruitment success in hemiepiphytic fig species[J]. Frontiers in Plant Science, 2021, 12: 754207.

[10]

Yan

, Liu

, Wu

K X

, et al. Comparative analysis of endogenous hormones and metabolite profiles in early-spring flowering plants and unflowered plants revealing the strategy of blossom[J]. Journal of Plant Growth Regulation, 2022, 41: 2421-2434.

[11]

Shareef

H J

, Al-Khayri

J M

. Photooxidative stress modulation of endogenous phytohormone and antioxidant accumulations and fruit maturity in date palm (Phoenix dactylifera L.)[J]. Journal of Plant Growth Regulation, 2020, 39: 1616-1624.

[12]

王湘莹, 黄兰清, 王晓明, 等. 不同施肥配比对紫薇叶片内源激素含量的影响[J]. 湖南林业科技, 2024, 51(4): 61-67.

Wang

X Y

, Huang

L Q

, Wang

X M

, et al. The effect of formula fertilization on the endogenous hormone content of leaves of Lagerstroemia indica[J]. Hunan Forestry Science & Technology, 2024, 51(4): 61-67.

[13]

Dziurka

, Dziurka

, Warchoł

, et al. Endogenous phytohormone profile during oat (Avena sativa L.) haploid embryo development[J]. In Vitro Cellular & Developmental Biology-Plant, 2019, 55: 221-229.

[14]

Lubna, Asaf

, Hamayum

, et al. Aspergillus niger CSR3 regulates plant endogenous hormones and secondary metabolites by producing gibberellins and indoleacetic acid[J]. Journal of Plant Interactions, 2018, 13: 100-111.

[15]

韦丽君, 俞奔驰, 宋恩亮, 等. 内源激素及氨基酸对木薯花芽分化的响应[J]. 西南农业学报, 2021, 34(7): 1400-1406.

Wei

L J

, Yu

B C

, Song

E L

, et al. Responses of endogenous hormones and amino acids to flower bud differentiation of cassava[J]. Southwest China Journal of Agricultural Sciences, 2021, 34(7): 1400-1406.

[16]

黄润生, 张晖, 廖柏勇, 等. 油茶花芽分化后期内源激素与代谢组学差异分析[J]. 经济林研究, 2021, 39(3): 99-113, 155.

Huang

R S

, Zhang

, Liao

B Y

, et al. Difference analysis of endogenous hormones and metabolomics in the late stage of flower buds differentiation of Camellia oleifera[J]. Non-wood Forest Research, 2021, 39(3): 99-113, 155.

[17]

石美丽, 宋西德. 叉子圆柏组织培养和快速繁殖研究[J]. 陕西林业科技, 2008, 36(2): 15-17, 27.

Shi

M L

, Song

X D

. Tissue culture and rapid propagation techniques of Sabina vulgaris[J]. Shaanxi Forest Science and Technology, 2008, 36(2): 15-17, 27.

[18]

章亮. 年龄对侧柏茎段培养快速繁殖的影响与黄帝手植柏克隆[D]. 杨凌: 西北农林科技大学, 2021: 18-19.

Zhang

. Effect of age on micropropagation of Platycladus orientalis and clone of the Platycladus orientalis tree plant by Huangdi[D]. Yangling: Northwest A & F University, 2021: 18-19.

[19]

张吴滔, 白卫国, 苏国芬, 等. 尾巨桉DH32-28组培再生过程中激素浓度筛选与转基因愈伤组织获得[J]. 桉树科技, 2024, 41(2): 1-10.

Zhang

W T

, Bai

W G

, Su

G F

, et al. Hormone concentrations transgenic calli production during tissue culture regeneration of Eucalyptus urophylla × E. grandis DH32-28[J]. Eucalypt Science & Technology, 2024, 41(2): 1-10.

[20]

黄华宏, 童再康, 廖望仪, 等. 香榧雌花芽部分内源激素的HPLC分析及动态变化[J]. 浙江林学院学报, 2005, 22(4): 390-395.

Huang

H H

, Tong

Z K

, Liao

W Y

, et al. High performance liquid chromatographic analysis and changes of endogenous hormones in female floral buds of Torreya grandis ‘Merrillii’[J]. Journal of Zhejiang Forestry College University, 2005, 22(4): 390-395.

[21]

朱松, 袁晓晴, 戴军, 等. QuEChERS/HPLC/DAD法同时检测果蔬中多种植物激素残留[J]. 分析测试学报, 2017, 36(4): 513-518.

Zhu

, Yuan

X Q

, Dai

, et al. Simultaneous determination of different phytohormones residues in fruits and vegetables by QuEChERS/DAD/HPLC[J]. Journal of Instrumental Analysis, 2017, 36(4): 513-518.

[22]

李雪, 李国泽, 杨玲, 等. 越桔属植物组织培养研究进展[J]. 植物生理学报, 2020, 56(5): 921-930.

, Li

G Z

, Yang

, et al. Research progress in tissue culture of Vaccinium plants[J]. Plant Physiology Communications, 2020, 56(5): 921-930.

[23]

周厚成, 赵霞, 李明, 等. 李砧木Marianna离体叶片再生体系优化研究[J]. 西北植物学报, 2011, 31(10): 2113-2118.

Zhou

H C

, Zhao

, Li

, et al. Optimizing system of plant regeneration from in vitro leaves of plum rootstock ‘Marianna’ (Prunus cerasifera×P.munsoniana)[J]. Acta Botanica Boreali-Occidentalia Sinica, 2011, 31(10): 2113-2118.

[24]

宋微, 张虎, 王磊, 等. 铁线莲‘Polish Spirit’组培快繁体系的建立[J]. 分子植物育种, 2020, 18(1): 261-267.

Song

, Zhang

, Wang

, et al. Clematis ‘Polish spirit’ establishment of tissue culture and rapid propagation system[J]. Molecular Plant Breeding, 2020, 18(1): 261-267.

[25]

郝梓博, 于水民, 张洪伟, 等. 承德地区红景天组培苗消毒条件筛选及启动培养研究[J]. 园艺与种苗, 2024, 44(3): 5-7.

Hao

Z B

, Yu

S M

, Zhang

H W

, et al. Disinfection conditions screening and initiation culture study of Rhodiola rosea tissue culture seedlings in Chengde area[J]. Horticulture & Seed, 2024, 44(3): 5-7.

[26]

王凯彬, 王晓敏, 王林, 等. 番茄幼胚离体培养影响因素的筛选[J]. 甘肃农业大学学报, 2025, 60(2): 163-171.

Wang

K B

, Wang

X M

, Wang

, et al. Study on the screening of factors affecting in vitro culture of immature embryos of tomato[J]. Journal of Gansu Agricultural University, 2025, 60(2): 163-171.

[27]

陈菲, 沈光, 曲彦婷, 等. 不同植物生长调节剂对橡胶草组培苗不定芽分化和生根的影响[J]. 北方园艺, 2017(19): 95-98.

Chen

, Shen

, Qu

Y T

, et al. Effects of different plant growth regulators on adventitious bud differentiation and rooting in tissue culture seedlings of Taraxacum koksaghyz[J]. Northern Horticulture, 2017(19): 95-98.

[28]

陈岑. 芦竹的组织培养研究[D]. 桂林: 广西师范大学, 2016: 33.

Chen

. Study on the tissue culture of Arundo donax[D]. Guilin: Guangxi Normal University, 2016: 33.

[29]

Mine

, Kitagami

, Tanikawa

, et al. Archaeal community structures associated with fine root systems of Cryptomeria japonica (Cupressaceae) in central Japan[J]. Journal of Forest Research, 2023, 28(1): 33-41.

[30]

冯代弟. 宝莲灯组培褐化作用机制的初步研究[D]. 合肥: 安徽农业大学, 2015: 21-23.

Feng

D D

. Preliminary study on browning mechanism of tissue culture of Medinilla magnifica[D]. Hefei: Anhui Agricultural University, 2015: 21-23.

[31]

高红兵, 杜凤国, 王欢. 抗褐化剂对天女木兰芽外植体褐化与酚酸氧化的影响[J]. 林业科学研究, 2017, 30(3): 525-532.

Gao

H B

, Du

F G

, Wang

, et al. Effects of browning inhibitors on bud explants browning and phenolic acids oxidation of Magnolia sieboldii[J]. Forest Research, 2017, 30(3): 525-532.

[32]

杨瑞玮. 林荫银莲花组织培养过程中褐变控制技术及相关机理研究[D]. 武汉: 华中农业大学, 2012: 30-31.

Yang

R W

. Studies on browning control technology and its mechanism in tissue culture of Anemone flaccida Fr. Schmidt[D]. Wuhan: Huazhong Agricultural University, 2012: 30-31.

[33]

Ilyas

, Nisar

, Khan

, et al. Drought tolerance strategies in plants: a mechanistic approach[J]. Journal of Plant Growth Regulation, 2021, 40: 926-944.

[34]

纪薇, 郭荣荣, 王静波, 等. 无核葡萄胚败育生理生化因子灰色关联分析[J]. 园艺学报, 2019, 46(8): 1473-1485.

, Guo

R R

, Wang

J B

, et al. Grey correlation analysis of physiological and biochemical factors in embryo abortion of seedless grape[J]. Acta Horticulturae Sinica, 2019, 46(8): 1473-1485.

[35]

Eichmann

, Richards

, Schafer

. Hormones as go-betweens in plant microbiome assembly[J]. The Plant Journal: for Cell and Molecular Biology, 2020, 105: 518-541.

[36]

Q Q

, Xu

, Chen

, et al. Synergistic interplay of ABA and BR signal in regulating plant growth and adaptation[J]. Nature Plants, 2021, 7: 1108-1118.

[37]

刘一灵, 李振华, 刘仁祥. 植物激素ABA调控种子发育与萌发的研究进展[J]. 种子, 2014, 33(10): 47-50.

Liu

Y L

, Li

Z H

, Liu

R X

. The research of ABA regulation in seed development and germination[J]. Seed, 2014, 33(10): 47-50.

[38]

Yang

, He

Y M

, et al. TMK1-based auxin signaling regulates abscisic acid responses via phosphorylating ABI1/2 in Arabidopsis[J]. Proceedings of the National Academy of Sciences of the United States of America, 2021, 118(24): e2102544118.

[39]

袁理春, 陈翠, 杨丽云, 等. 温度和赤霉素对滇重楼种子二次发育的影响[J]. 种子, 2003, 22(5): 33-34.

Yuan

L C

, Chen

, Yang

L Y

, et al. Effects of temperature and gibberellin treatments on the second growth of seeds of Paris polyphylla var. yunnanensis[J]. Seed, 2003, 22(5): 33-34.

[40]

潘琼玉. 苦瓜离体再生体系的建立及不定芽再生机理初步研究[D]. 海口: 海南大学, 2019: 34-35.

Pan

Q Y

. The establishment of in vitro regeneration system of bitter gourd and preliminary study on the mechanism of adventitious bud regeneration[D]. Haikou: Hainan University, 2019: 34-35.

[41]

周双, 孙伟, 杨光, 等. 绥李3号李叶芽萌发过程中内源激素含量的变化[J]. 中国果树, 2022(1): 45-49.

Zhou

, Sun

, Yang

, et al. Changes of endogenous hormones content in leaf buds of ‘Suili 3’[J]. China Fruits, 2022(1): 45-49.

[42]

张慧君, 栾非时, 王学征, 等. 甜瓜自交系不定芽再生过程中内源激素含量的变化[J]. 中国蔬菜, 2012(24): 43-47.

Zhang

H J

, Luan

F S

, Wang

X Z

, et al. Changes of endogenous hormone contents during in vitro regeneration of adventitious bud of Cucumis melo L. inbred line[J]. China Vegetables, 2012(24): 43-47.

[43]

Prasad

. Cytokinin and its key role to enrich the plant nutrients and growth under adverse conditions-an update[J]. Frontiers in Genetics, 2022, 13: 883924.

[44]

Schaller

G E

, Bishopp

, Kieber

J J

. The Yin-Yang of hormones: cytokinin and auxin interactions in plant development[J]. The Plant Cell, 2015, 27: 44-63.

[45]

朱宝, 赵江哲, 张可伟, 等. 水稻细胞分裂素氧化酶9参与调控水稻叶夹角发育[J]. 植物学报, 2024, 59(1): 10-21.

Zhu

, Zhao

J Z

, Zhang

K W

, et al. OsCKX9 is involved in regulating the rice Lamina joint development and leaf angle[J]. Bulletin of Botany, 2024, 59(1): 10-21.

2026年第46卷第8期

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文章信息

doi: 10.14067/j.cnki.1673-923x.2026.08.005

接收时间：2025-07-19
首发时间：2026-10-30
出版时间：2026-08-25

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出版历史

收稿日期：2025-07-19
修回日期：2025-10-09

基金

福建省林业种苗科技攻关项目(ZMGG-0811)

福建农林大学科技创新专项(KFB23057)

作者信息

^a.福建农林大学　林学院，福建　福州　350000

^b.福建农林大学　风景园林与艺术学院，福建　福州　350000

通讯作者:

陈礼光（fjclg@qq.com），副教授，博士，硕士研究生导师。

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https://castjournals.cast.org.cn/joweb/znlykj/CN/10.14067/j.cnki.1673-923x.2026.08.005

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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