Article(id=1322924664934249221, tenantId=1146029695717560320, journalId=1322258771877134531, issueId=1322924574345670676, articleNumber=null, orderNo=null, doi=10.14067/j.cnki.1673-923x.2026.07.020, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1753891200000, receivedDateStr=2025-07-31, revisedDate=1760889600000, revisedDateStr=2025-10-20, acceptedDate=null, acceptedDateStr=null, onlineDate=1793339418872, onlineDateStr=2026-10-30, pubDate=1784908800000, pubDateStr=2026-07-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1793339418872, onlineIssueDateStr=2026-10-30, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1793339418872, creator=13701087609, updateTime=1793339418872, updator=13701087609, issue=Issue{id=1322924574345670676, tenantId=1146029695717560320, journalId=1322258771877134531, year='2026', volume='46', issue='7', pageStart='1', pageEnd='234', issueExtLink='null', onlineDate='null', pubDate='1784908800000', pubDateStr='2026-07-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1793339397274, creator='13701087609', updateTime=1793344662547, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1322946659029746602, tenantId=1146029695717560320, journalId=1322258771877134531, issueId=1322924574345670676, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1322946659029746603, tenantId=1146029695717560320, journalId=1322258771877134531, issueId=1322924574345670676, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=216, endPage=224, ext={EN=ArticleExt(id=1322924665089438470, articleId=1322924664934249221, tenantId=1146029695717560320, journalId=1322258771877134531, language=EN, title=Cloning and functional analysis of phe-novel-miR276 in
Phyllostachys edulis, columnId=null, journalTitle=Journal of Central South University of Forestry & Technology, columnName=null, runingTitle=null, highlight=null, articleAbstract=
【Objective】 To explore the regulatory effect of novel miRNA phe-novel-miR276 on lignin synthesis in moso bamboo, so as to provide theoretical reference for material quality improvement and molecular breeding of moso bamboo.
【Method】 Using bioinformatics methods, we analyzed the novel differentially expressed miRNA (phe-novel-miR276) screened in the preliminary study of moso bamboo. qRT-PCR was applied to detect its expression patterns in different moso bamboo tissues, followed by calculation of relative expression levels and differential analysis. The phe-novel-miR276 was cloned to construct an overexpression plant vector, which was transformed into rice via Agrobacterium mediation for screening positive transgenic lines. Lignin content as well as POD and SOD activities of transgenic and wild-type rice were determined, and intergroup differences of each index were compared and analyzed.
【Result】 1) Sequence analysis revealed that phe-novel-miR276 shares high homology with the miR398 family, suggesting it is a new member of the miR398 family; 2) phe-novel-miR276 is expressed in the roots, stems, and leaves of P. edulis, with the highest relative expression level in the roots; 3) Phloroglucinol staining showed that the lignin staining depth in the stems and leaves of transgenic lines was lighter; meanwhile, the results of the microdetermination method indicated that the total lignin content in the stems and leaves of transgenic lines was significantly reduced; 4) qRT-PCR detection demonstrated that, compared with wild-type rice, the expression level of Os10g36848-the rice homologous target gene of phe-novel-miR276 (encoding ferulate 5-hydroxylase, F5H)-was significantly downregulated in transgenic lines. Additionally, the POD and SOD activities in transgenic lines were significantly higher than those in wild-type rice, respectively.
【Conclusion】 In P. edulis, phe-novel-miR276 exerts its regulatory role by downregulating the expression of lignin biosynthesis-related target genes, and indirectly affects the activity of the antioxidant enzyme system in plants.
, authors=Li Han, Yuanmeng Gao, Xiaofang Lin, Ying Li, Xueping Li, authorsList=Li Han, Yuanmeng Gao, Xiaofang Lin, Ying Li, Xueping Li, authorCompany=null, correspAuthors=Xueping Li, authorNote=null, correspAuthorsNote=null, copyrightStatement=© 2026 Journal of Central South University of Forestry & Technology, copyrightOwner=Journal of Central South University of Forestry & Technology, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1322924665353679623, articleId=1322924664934249221, tenantId=1146029695717560320, journalId=1322258771877134531, language=CN, title=毛竹phe-novel-miR276的克隆和功能分析, columnId=1322924272984929210, journalTitle=中南林业科技大学学报, columnName=生物学, runingTitle=null, highlight=null, articleAbstract=
【目的】 探究毛竹新miRNA phe-novel-miR276对木质素合成的调控作用,为毛竹材质改良与分子育种提供理论参考。
【方法】 采用生物信息学方法分析前期筛选获得的毛竹差异表达的新miRNA(phe-novel-miR276);通过qRT-PCR检测其在毛竹不同组织中的表达模式,计算相对表达量并进行差异分析;克隆phe-novel-miR276,构建过表达植物表达载体,经农杆菌介导转化水稻并筛选阳性转基因株系;测定转基因水稻与野生型水稻的木质素含量及POD、SOD活性,对比分析各项指标的组间差异。
【结果】 1)序列分析表明,phe-novel-miR276与miR398家族具有高度同源性,推测其为miR398家族的新成员;2)phe-novel-miR276在毛竹根、茎和叶中均有表达,且根部相对表达量最高;3)间苯三酚染色显示转基因株系茎、叶木质素染色深度变浅,微量法测定木质素含量的结果表明其茎、叶木质素总含量显著降低;4)qRT-PCR检测显示,与野生型水稻相比,转基因株系中phe-novel-miR276的水稻同源靶基因Os10g36848(编码F5H)表达量显著下调。此外,转基因株系POD和SOD活性分别显著高于野生型。
【结论】 在毛竹中,phe-novel-miR276可通过下调靶向调控木质素合成相关基因的表达发挥调控作用,并间接影响植株抗氧化酶系统活性。
, authors=韩莉, 高圆梦, 林小芳, 李英, 李雪平, authorsList=韩莉, 高圆梦, 林小芳, 李英, 李雪平, authorCompany=null, correspAuthors=李雪平, authorNote=
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