中南林业科技大学学报

新疆野苹果LEA基因家族鉴定与表达分析

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房震 , 李静 , 李美芸 , 旷莹香 , 叶春秀

中南林业科技大学学报 | 生物学 2026,46(6): 184-199

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中南林业科技大学学报 |生物学 2026 , 46 (6) : 184 -199

新疆野苹果LEA基因家族鉴定与表达分析

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房震, 李静, 李美芸, 旷莹香, 叶春秀

作者信息

新疆农业大学　林学与风景园林学院，新疆　乌鲁木齐　830052

通讯作者:

叶春秀（yecx2008@163.com），副教授，博士，硕士研究生导师。

作者简介:

房震（fz199971@163.com），硕士研究生。

Identification and expression analysis of LEA gene family in Malus sieversii

Zhen FANG, Jing LI, Meiyun LI, Yingxiang KUANG, Chunxiu YE

Affiliations

College of Forestry and Landscape Architecture, Xinjiang Agricultural University, Urumqi 830052, Xinjiang, China

出版时间: 2026-06-25 doi: 10.14067/j.cnki.1673-923x.2026.06.018

文章导航

摘要

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【目的】

全面解析晚期胚胎发生丰富蛋白在高等植物胚胎发育和非生物胁迫反应中的调控机制。

【方法】

利用隐马尔可夫模型和生物信息学方法，从新疆野苹果全基因组中筛选鉴定LEA基因家族，对其序列特征、保守基序、基因结构、顺式作用元件和表达模式等进行分析。

【结果】

新疆野苹果全基因组中共鉴定出119个LEA基因。根据它们的保守结构域和系统发育关系，共分为8个不同的组（LEA1-LEA6，DHN，SMP），其中MsiLEA2组的基因最丰富。顺式作用元件的分析结果表明，大多数MsiLEA基因与光响应元件、非生物胁迫响应相关。此外，一些基因与生物合成途径有关，如脱落酸响应、生长激素响应、茉莉酸甲酯响应和分生组织表达等。新疆野苹果LEA基因在种子低温层积的不同时期和不同器官中的表达模式分析显示，MsiDHN3在种子低温层积过程中表达量下降，在所有器官中表达量较高，表明MsiDHN3在种子低温层积过程中发挥作用。

【结论】

本研究为新疆野苹果MsiLEA基因家族的形成提供了新见解，同时也加深了对这些基因在新疆野苹果种子胚胎发育阶段和非生物胁迫响应过程中的潜在作用的理解。这些结果对今后MsiLEA基因家族的功能研究具有一定的指导意义，并为探索MsiLEA基因在新疆野苹果种子胚胎发育阶段的作用提供理论依据。

关键词

新疆野苹果 / LEA基因家族 / 全基因组鉴定 / 表达分析

Abstract

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【Objective】

To comprehensively elucidate the regulatory mechanisms of late embryogenesis abundant (LEA) proteins in higher plant embryogenesis and abiotic stress responses.

【Method】

Hidden Markov model and bioinformatics were used to screen and identify LEA genes family from the whole genome of Malus sieversii, and their sequence features, conserved motifs, gene structures, cis-acting elements, and expression patterns were analyzed.

【Result】

The 119 LEA genes were identified in the whole genome of Malus sieversii. According to their conserved structural domains and phylogenetic relationships, they were categorized into eight different groups (LEA1-LEA6, DHN, SMP), among which the MsiLEA2 group was the most gene-rich. Analysis of the cis-acting elements showed that most of the MsiLEA genes were associated with light response elements, abiotic stress response. In addition, some genes were related to biosynthetic pathways, such as abscisic acid response, growth hormone response, methyl jasmonate response, and meristematic tissue expression. The analysis of the expression pattern of Malus sieversii LEA genes in different periods of seed low-temperature stratification and in different organs showed that the expression of MsiDHN3 decreased during seed low-temperature stratification and was higher in all organs, indicating that MsiDHN3 plays a role in the low-temperature stratification of Malus sieversii seeds.

【Conclusion】

This study provides new insights into the formation of the MsiLEA genes family in Malus sieversii, it also deepens the understanding of the potential roles of these genes during embryonic developmental stages and abiotic stress response in Malus sieversii seeds. These results are instructive for future functional studies of the MsiLEA genes family and provide a theoretical basis for exploring the role of MsiLEA genes in the embryonic developmental stages of Malus sieversii seeds.

Key words

Malus sieversii / LEA gene family / genome-wide identification / expression analysis

引用本文

房震, 李静, 李美芸, 旷莹香, 叶春秀. 新疆野苹果LEA基因家族鉴定与表达分析. 中南林业科技大学学报, 2026 , 46 (6) : 184 -199 . DOI: 10.14067/j.cnki.1673-923x.2026.06.018

Zhen FANG, Jing LI, Meiyun LI, Yingxiang KUANG, Chunxiu YE. Identification and expression analysis of LEA gene family in Malus sieversii[J]. Journal of Central South University of Forestry & Technology, 2026 , 46 (6) : 184 -199 . DOI: 10.14067/j.cnki.1673-923x.2026.06.018

基金

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新疆维吾尔自治区自然科学基金面上项目(2023D01A28)

参考文献

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2026年第46卷第6期

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doi: 10.14067/j.cnki.1673-923x.2026.06.018

接收时间：2025-06-03
首发时间：2026-10-30
出版时间：2026-06-25

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收稿日期：2025-06-03
修回日期：2025-08-21

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新疆维吾尔自治区自然科学基金面上项目(2023D01A28)

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新疆农业大学　林学与风景园林学院，新疆　乌鲁木齐　830052

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叶春秀（yecx2008@163.com），副教授，博士，硕士研究生导师。

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2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

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