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Article(id=1292765503899071404, tenantId=1146029695717560320, journalId=1291416979304972313, issueId=1291824268032110598, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1005-4847.2026.03.003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1749484800000, receivedDateStr=2025-06-10, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1786148914550, onlineDateStr=2026-08-08, pubDate=1774800000000, pubDateStr=2026-03-30, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1786148914550, onlineIssueDateStr=2026-08-08, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1786148914550, creator=13701087609, updateTime=1786148914550, updator=13701087609, issue=Issue{id=1291824268032110598, tenantId=1146029695717560320, journalId=1291416979304972313, year='2026', volume='34', issue='3', pageStart='313', pageEnd='468', issueExtLink='null', onlineDate='null', pubDate='1774800000000', pubDateStr='2026-03-30', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1785924506432, creator='ztmeta', updateTime=1786331936953, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1293533155567366441, tenantId=1146029695717560320, journalId=1291416979304972313, issueId=1291824268032110598, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1293533155567366442, tenantId=1146029695717560320, journalId=1291416979304972313, issueId=1291824268032110598, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=339, endPage=351, ext={EN=ArticleExt(id=1292765504108786605, articleId=1292765503899071404, tenantId=1146029695717560320, journalId=1291416979304972313, language=EN, title=Anti-osteoporosis effect and mechanism of calcium zinc vitamin oral liquid in zebrafish and its absorption characteristics in Caco-2 cells, columnId=null, journalTitle=Acta Laboratorium Animalis Scientia Sinica, columnName=null, runingTitle=null, highlight=null, articleAbstract=
Objective

To determine the absorption rate and anti-osteoporosis efficacy of calcium zinc vitamin D vitamin K oral solution (YQJ) and its components in Caco-2 cells and zebrafish, and explore its potential mechanism based on transcriptome sequencing.

Methods

We compared the calcium-ion absorption rates of YQJ and its components in Caco-2 cells, to simulate gastrointestinal absorption. An osteoporosis model was induced using dexamethasone. Bone density was detected by Alizarin Red staining and dual validation of drug efficacy was analyzed using transgenic (ola. sp7: nlsGFP) osteoblast fluorescence. Zebrafish were divided into YQJ 250 μg/mL, MC, and NC groups (3 biological replicates each). Transcriptome sequencing was carried out and differentially expressed genes at the intersection of the three groups were screened using | log2 fold-change| ≥ 2 and Q value ≤ 0.001. Differential genes underwent functional annotation and enrichment analyses to screen out the expression pathway of YQJ anti-osteoporosis genes, followed by quantitative polymerase chain reaction validation.

Results

The absolute calcium-absorption rate in YQJ-treated Caco-2 cells was 42.6%, which was 1.70 fold higher than that of the calcium citrate-alone group, 1.59, 1.58, and 1.56 fold higher than the calcium citrate + vitamin (V) D3, calcium citrate + VK2, and calcium citrate + (casein phosphopeptides) CPP groups, respectively, and 1.33 fold higher than the calcium citrate + zinc gluconate + D3 group, indicating a significant synergistic effect of YQJ on promoting calcium absorption. YQJ significantly restored zebrafish skull density and increased osteogenic fluorescence intensity, and 125 μg/mL YQJ increased the anti-osteoporosis effect by 24% ~ 25% compared with calcium citrate alone. Sequencing identified 335 and 231 differentially expressed genes in MC group/NC group and YQJ group/MC group, respectively, with 58 overlapping genes at the intersection of the three groups. Functional enrichment of overlapping genes revealed that YQJ's anti-osteoporosis effect involved four pathways and 11 key genes. Using qPCR verification, YQJ significantly restored the mRNA expression levels of c3a. 1, cp, coro1a, cldni, and bmpr1aa.

Conclusions

The in vitro calcium-absorption rate and anti-osteoporosis efficacy of YQJ both demonstrated synergistic effects, with its anti-osteoporosis effect specifically increasing skull density and osteoblast signaling intensity. Transcriptome sequencing revealed that YQJ primarily functions by modulating the complement and coagulation cascade, ferroptosis, phagosome, oxidative stress response signaling pathways, and other functional proteins, involving the regulation of key genes such as c3a. 1, c3a. 2, c3a. 6, c9, c5, cfb, coro1a, cp, mpx, cldni, and bmpr1aa.

, authors=Zumeng XIA1, Xutian KAN1, *, Qinwen WANG2, Weichao HUANG1, Guanming LI2, Mingzhu DAI2, Lijuan WANG3, authorsList=Zumeng XIA, Xutian KAN, Qinwen WANG, Weichao HUANG, Guanming LI, Mingzhu DAI, Lijuan WANG, authorCompany=null, correspAuthors=Xutian KAN, authorNote=null, correspAuthorsNote=
KAN Xutian. E-mail:
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目的

对YQJ钙锌维生素D维生素K口服液（简称“YQJ”）及其拆方进行体外吸收率和斑马鱼抗骨质疏松功效检测，基于转录组测序术探讨潜在作用机制。

方法

利用Caco-2细胞模拟胃肠道吸收，比较YQJ及其拆方钙离子吸收率。使用地塞米松诱导骨质疏松模型，通过茜素红钙染骨密度及Tg（ola. sp7：nlsGFP）成骨细胞荧光双重验证药效。设置YQJ 250 μg/mL组、MC组和NC组，均3个生物学重复，对斑马鱼进行转录组测序，以|log2fold-change| ≥ 2、Q value ≤ 0.001严格筛选3组交集的差异基因，进行功能注释和富集分析，筛出YQJ抗骨质疏松基因的表达通路，并进行qPCR验证。

结果

在Caco-2肠屏障体外模型中，YQJ组钙离子吸收率为42.6%，相比为单一柠檬酸钙组吸收率的1.70倍，为柠檬酸钙 + VD3组、柠檬酸钙 +VK2组、柠檬酸钙 + CPP组的1.59、1.58、1.56倍，为柠檬酸钙 + 葡萄糖酸锌 + VD3组的1.33倍，产品YQJ协同促进钙吸收效果显著。YQJ能显著恢复斑马鱼头骨密度、提高成骨荧光强度，且同125 μg/mL浓度下YQJ抗骨质疏松功效较单一柠檬酸钙增加24% ~ 25%。测序结果MC组/NC组、YQJ组/MC组差异基因为340、231个，3组交集58个重叠。对重叠基因进行功能富集，YQJ抗骨质疏松涉及4条通路和11个关键基因。利用qPCR验证，YQJ可明显恢复c3a. 1、cp、coro1a、cldni、bmpr1aa mRNA的表达水平。

结论

YQJ的体外钙吸收率及抗斑马鱼骨质疏松功效均呈现出了协同增效作用，其中抗斑马鱼骨质疏松功效具体指YQJ可增加头骨密度和成骨细胞信号强度。转录组测序发现YQJ主要是通过调节补体和凝血级联反应、铁死亡、吞噬体、氧化应激反应信号通路和其他功能蛋白发挥作用，具体涉及c3a. 1、c3a. 2、c3a. 6、c9、c5、cfb、coro1a、cp、mpx、cldni和bmpr1aa关键基因调控。

, authors=夏祖猛1, 阚绪甜1, *, 王钦文2, 黄炜超1, 李观明2, 戴明珠2, 王丽娟3, authorsList=夏祖猛, 阚绪甜, 王钦文, 黄炜超, 李观明, 戴明珠, 王丽娟, authorCompany=null, correspAuthors=阚绪甜, authorNote=

夏祖猛，男，硕士，研究方向：健康食品技术研究和产品开发。Email：

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阚绪甜，男，硕士，研究方向：健康食品技术研究及产品开发。Email：
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夏祖猛，男，硕士，研究方向：健康食品技术研究和产品开发。Email：

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夏祖猛，男，硕士，研究方向：健康食品技术研究和产品开发。Email：

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钙锌维生素口服液抗斑马鱼骨质疏松作用和机制及其在Caco-2细胞中吸收特性研究
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夏祖猛 1 , 阚绪甜 1, * , 王钦文 2 , 黄炜超 1 , 李观明 2 , 戴明珠 2 , 王丽娟 3
中国实验动物学报 | 研究报告 2026,34(3): 339-351
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中国实验动物学报 |研究报告 2026 , 34 (3) : 339 -351
钙锌维生素口服液抗斑马鱼骨质疏松作用和机制及其在Caco-2细胞中吸收特性研究
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夏祖猛1 , 阚绪甜1, * , 王钦文2, 黄炜超1, 李观明2, 戴明珠2, 王丽娟3
作者信息
  • 1．无限极（中国）有限公司，广州　510403
  • 2．杭州环特生物科技股份有限公司，杭州　310051
  • 3．中国疾病预防控制中心营养与健康所，北京　102211
通讯作者:
阚绪甜，男，硕士，研究方向：健康食品技术研究及产品开发。Email：
作者简介:

夏祖猛，男，硕士，研究方向：健康食品技术研究和产品开发。Email：

Anti-osteoporosis effect and mechanism of calcium zinc vitamin oral liquid in zebrafish and its absorption characteristics in Caco-2 cells
Zumeng XIA1 , Xutian KAN1, * , Qinwen WANG2, Weichao HUANG1, Guanming LI2, Mingzhu DAI2, Lijuan WANG3
Affiliations
  • 1.Infinitus (China) Company Ltd., Guangzhou 510403, China
  • 2.Hunter Biotechnology, Inc., Hangzhou 310051, China
  • 3.National Institute for Nutrition and Health, Chinese Center for Disease Control and Prevention, Beijing 102211, China
    • Corresponding Author:
    KAN Xutian. E-mail:
出版时间: 2026-03-30 doi: 10.3969/j.issn.1005-4847.2026.03.003
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摘要
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目的

对YQJ钙锌维生素D维生素K口服液（简称“YQJ”）及其拆方进行体外吸收率和斑马鱼抗骨质疏松功效检测，基于转录组测序术探讨潜在作用机制。

方法

利用Caco-2细胞模拟胃肠道吸收，比较YQJ及其拆方钙离子吸收率。使用地塞米松诱导骨质疏松模型，通过茜素红钙染骨密度及Tg（ola. sp7：nlsGFP）成骨细胞荧光双重验证药效。设置YQJ 250 μg/mL组、MC组和NC组，均3个生物学重复，对斑马鱼进行转录组测序，以|log2fold-change| ≥ 2、Q value ≤ 0.001严格筛选3组交集的差异基因，进行功能注释和富集分析，筛出YQJ抗骨质疏松基因的表达通路，并进行qPCR验证。

结果

在Caco-2肠屏障体外模型中，YQJ组钙离子吸收率为42.6%，相比为单一柠檬酸钙组吸收率的1.70倍，为柠檬酸钙 + VD3组、柠檬酸钙 +VK2组、柠檬酸钙 + CPP组的1.59、1.58、1.56倍，为柠檬酸钙 + 葡萄糖酸锌 + VD3组的1.33倍，产品YQJ协同促进钙吸收效果显著。YQJ能显著恢复斑马鱼头骨密度、提高成骨荧光强度，且同125 μg/mL浓度下YQJ抗骨质疏松功效较单一柠檬酸钙增加24% ~ 25%。测序结果MC组/NC组、YQJ组/MC组差异基因为340、231个，3组交集58个重叠。对重叠基因进行功能富集，YQJ抗骨质疏松涉及4条通路和11个关键基因。利用qPCR验证，YQJ可明显恢复c3a. 1、cp、coro1a、cldni、bmpr1aa mRNA的表达水平。

结论

YQJ的体外钙吸收率及抗斑马鱼骨质疏松功效均呈现出了协同增效作用，其中抗斑马鱼骨质疏松功效具体指YQJ可增加头骨密度和成骨细胞信号强度。转录组测序发现YQJ主要是通过调节补体和凝血级联反应、铁死亡、吞噬体、氧化应激反应信号通路和其他功能蛋白发挥作用，具体涉及c3a. 1、c3a. 2、c3a. 6、c9、c5、cfb、coro1a、cp、mpx、cldni和bmpr1aa关键基因调控。

斑马鱼  /  钙吸收  /  骨质疏松  /  Caco-2细胞  /  转录组学
Objective

To determine the absorption rate and anti-osteoporosis efficacy of calcium zinc vitamin D vitamin K oral solution (YQJ) and its components in Caco-2 cells and zebrafish, and explore its potential mechanism based on transcriptome sequencing.

Methods

We compared the calcium-ion absorption rates of YQJ and its components in Caco-2 cells, to simulate gastrointestinal absorption. An osteoporosis model was induced using dexamethasone. Bone density was detected by Alizarin Red staining and dual validation of drug efficacy was analyzed using transgenic (ola. sp7: nlsGFP) osteoblast fluorescence. Zebrafish were divided into YQJ 250 μg/mL, MC, and NC groups (3 biological replicates each). Transcriptome sequencing was carried out and differentially expressed genes at the intersection of the three groups were screened using | log2 fold-change| ≥ 2 and Q value ≤ 0.001. Differential genes underwent functional annotation and enrichment analyses to screen out the expression pathway of YQJ anti-osteoporosis genes, followed by quantitative polymerase chain reaction validation.

Results

The absolute calcium-absorption rate in YQJ-treated Caco-2 cells was 42.6%, which was 1.70 fold higher than that of the calcium citrate-alone group, 1.59, 1.58, and 1.56 fold higher than the calcium citrate + vitamin (V) D3, calcium citrate + VK2, and calcium citrate + (casein phosphopeptides) CPP groups, respectively, and 1.33 fold higher than the calcium citrate + zinc gluconate + D3 group, indicating a significant synergistic effect of YQJ on promoting calcium absorption. YQJ significantly restored zebrafish skull density and increased osteogenic fluorescence intensity, and 125 μg/mL YQJ increased the anti-osteoporosis effect by 24% ~ 25% compared with calcium citrate alone. Sequencing identified 335 and 231 differentially expressed genes in MC group/NC group and YQJ group/MC group, respectively, with 58 overlapping genes at the intersection of the three groups. Functional enrichment of overlapping genes revealed that YQJ's anti-osteoporosis effect involved four pathways and 11 key genes. Using qPCR verification, YQJ significantly restored the mRNA expression levels of c3a. 1, cp, coro1a, cldni, and bmpr1aa.

Conclusions

The in vitro calcium-absorption rate and anti-osteoporosis efficacy of YQJ both demonstrated synergistic effects, with its anti-osteoporosis effect specifically increasing skull density and osteoblast signaling intensity. Transcriptome sequencing revealed that YQJ primarily functions by modulating the complement and coagulation cascade, ferroptosis, phagosome, oxidative stress response signaling pathways, and other functional proteins, involving the regulation of key genes such as c3a. 1, c3a. 2, c3a. 6, c9, c5, cfb, coro1a, cp, mpx, cldni, and bmpr1aa.

zebrafish  /  calcium absorption  /  osteoporosis  /  Caco-2 cells  /  transcriptomics
夏祖猛, 阚绪甜, 王钦文, 黄炜超, 李观明, 戴明珠, 王丽娟. 钙锌维生素口服液抗斑马鱼骨质疏松作用和机制及其在Caco-2细胞中吸收特性研究. 中国实验动物学报, 2026 , 34 (3) : 339 -351 . DOI: 10.3969/j.issn.1005-4847.2026.03.003
Zumeng XIA, Xutian KAN, Qinwen WANG, Weichao HUANG, Guanming LI, Mingzhu DAI, Lijuan WANG. Anti-osteoporosis effect and mechanism of calcium zinc vitamin oral liquid in zebrafish and its absorption characteristics in Caco-2 cells[J]. Acta Laboratorium Animalis Scientia Sinica, 2026 , 34 (3) : 339 -351 . DOI: 10.3969/j.issn.1005-4847.2026.03.003
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  • 国家重点研发计划(2023YFF1103703)
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2026年第34卷第3期
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doi: 10.3969/j.issn.1005-4847.2026.03.003
  • 接收时间：2025-06-10
  • 首发时间：2026-08-08
  • 出版时间：2026-03-30
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  • 收稿日期：2025-06-10
基金
National Key Research and Development Program(2023YFF1103703)
国家重点研发计划(2023YFF1103703)
作者信息
    1．无限极（中国）有限公司，广州　510403
    2．杭州环特生物科技股份有限公司，杭州　310051
    3．中国疾病预防控制中心营养与健康所，北京　102211

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阚绪甜，男，硕士，研究方向：健康食品技术研究及产品开发。Email：
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https://castjournals.cast.org.cn/joweb/zgsydwxb/CN/10.3969/j.issn.1005-4847.2026.03.003
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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