Article(id=1307407752972759134, tenantId=1146029695717560320, journalId=1306273872116547672, issueId=1307407751768986283, articleNumber=null, orderNo=null, doi=10.16819/j.1001-7216.2026.251016, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1761667200000, receivedDateStr=2025-10-29, revisedDate=1769356800000, revisedDateStr=2026-01-26, acceptedDate=null, acceptedDateStr=null, onlineDate=1789639898770, onlineDateStr=2026-09-17, pubDate=1783612800000, pubDateStr=2026-07-10, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1789639898770, onlineIssueDateStr=2026-09-17, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1789639898770, creator=13701087609, updateTime=1789639898770, updator=13701087609, issue=Issue{id=1307407751768986283, tenantId=1146029695717560320, journalId=1306273872116547672, year='2026', volume='40', issue='4', pageStart='425', pageEnd='568', issueExtLink='null', onlineDate='null', pubDate='1783612800000', pubDateStr='2026-07-10', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1789639898482, creator='13701087609', updateTime=1790746138035, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1312047656814343051, tenantId=1146029695717560320, journalId=1306273872116547672, issueId=1307407751768986283, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1312047656814343052, tenantId=1146029695717560320, journalId=1306273872116547672, issueId=1307407751768986283, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=447, endPage=459, ext={EN=ArticleExt(id=1307407753195057247, articleId=1307407752972759134, tenantId=1146029695717560320, journalId=1306273872116547672, language=EN, title=Identification and Functional Analysis of the Rice Vein Development Gene OsLVD1, columnId=1307407385149071973, journalTitle=Chinese Journal of Rice Science, columnName=Research Papers, runingTitle=null, highlight=null, articleAbstract=
【Objective】

Photosynthesis is a major determinant of dry matter accumulation in rice, with chlorophyll content and vein density being critical for photosynthetic efficiency. Here, a rice gene regulating chlorophyll content and vein density, OsLVD1, was identified and functionally analyzed.

【Method】

OsLVD1 was identified based on homology to maize vein development genes, and its protein features, phylogeny, conserved motifs, and subcellular localization were analyzed bioinformatically. An lvd1 mutant was generated using CRISPR/Cas9, with ZH11 as the wild type. Chlorophyll content, vein density, soluble sugar content, and major agronomic traits were examined. The expression of genes related to chlorophyll metabolism and vein development was analyzed by qRT-PCR.

【Result】

OsLVD1 encodes a nuclear-localized WRKY transcription factor, and the lvd1 mutation caused a frameshift leading to premature termination. Compared with ZH11, the lvd1 mutant showed reductions of 25.90%, 30.75%, and 27.05% in chlorophyll a, chlorophyll b, and total chlorophyll content, respectively, accompanied by increases in minor vein density and bulliform cell number. Plant height was reduced and growth duration was slightly delayed, whereas soluble sugar content, seed-setting rate, and thousand-grain weight were unaffected. qRT-PCR showed that the expression levels of chlorophyll biosynthesis genes OsHEMA1 and OsCHLI were downregulated, while those of the chlorophyll degradation gene OsNYC1 and the vein development gene OsPIN1b were upregulated in the lvd1 mutant. OsLVD1 was predominantly expressed in leaves, with the highest expression at the jointing stage.

【Conclusion】

Loss of OsLVD1 resulted in a "low chlorophyll-high vein density" phenotype, which may compensate for reduced photosynthesis by enhancing vein-mediated transport and water retention, thereby maintaining yield stability. This study demonstrates a novel role of OsLVD1 in coordinating leaf pigment metabolism and vascular development in rice.

, authors=Yijie QIU, Feiyang QI, Hewei DU, Min HUANG, Manfei LI, authorsList=Yijie QIU, Feiyang QI, Hewei DU, Min HUANG, Manfei LI, authorCompany=null, correspAuthors=Min HUANG, Manfei LI, authorNote=null, correspAuthorsNote=

*

email:
, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1307407753421549664, articleId=1307407752972759134, tenantId=1146029695717560320, journalId=1306273872116547672, language=CN, title=水稻叶脉基因OsLVD1的鉴定及功能分析, columnId=1307407385430090343, journalTitle=中国水稻科学, columnName=研究报告, runingTitle=null, highlight=null, articleAbstract=
【目的】

光合作用决定水稻干物质积累,叶绿素含量和叶脉密度与光合作用密切相关。本研究在水稻中鉴定调控叶绿素含量和叶脉密度的基因OsLVD1,并对其功能进行解析。

【方法】

通过玉米叶脉发育基因同源比对获得OsLVD1,并利用生物信息学分析其蛋白特性、系统发育关系、信号肽、跨膜结构域、保守结构域及亚细胞定位。通过CRISPR获得lvd1编辑株,以野生型ZH11为对照,测定叶绿素含量、叶脉密度、可溶性糖含量及株高、分蘖数、每穗粒数等主要农艺性状,并通过qRT-PCR分析相关基因的表达水平。

【结果】

生物信息学分析表明,OsLVD1编码WRKY家族转录因子,定位于细胞核。lvd1因移码突变导致蛋白提前终止。与ZH11相比,lvd1叶绿素a、叶绿素b和总叶绿素分别下降25.90%、30.75%和27.05%,剑叶小脉密度和泡状细胞数目增加。lvd1株高降低且生育期略延迟,但叶片可溶性糖、结实率、千粒重等均无显著差异。qRT-PCR分析表明,lvd1中叶绿素合成相关基因OsHEMA1和OsCHLI的表达显著下调,而叶绿素降解基因OsNYC1及叶脉发育相关基因OsPIN1b表达上调。组织表达分析显示,OsLVD1在叶片中高表达,且在拔节期达到峰值。

【结论】

OsLVD1功能缺失导致叶绿素含量降低、叶脉密度增加,可能通过增加叶脉密度和改变泡状细胞发育补偿光合产物运输与水分保持,从而维持产量稳定。本研究揭示OsLVD1在叶片色素代谢与维管系统发育中的新功能,为光合结构优化提供理论基础。

, authors=邱一洁, 齐飞扬, 杜何为, 黄敏*, *, 李曼菲*, *, authorsList=邱一洁, 齐飞扬, 杜何为, 黄敏, 李曼菲, authorCompany=null, correspAuthors=黄敏, 李曼菲, authorNote=null, correspAuthorsNote=

*

email:
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水稻叶脉基因OsLVD1的鉴定及功能分析
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邱一洁 , 齐飞扬 , 杜何为 , 黄敏 *, * , 李曼菲 *, *
中国水稻科学 | 研究报告 2026,40(4): 447-459
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中国水稻科学 |研究报告 2026 , 40 (4) : 447 -459
水稻叶脉基因OsLVD1的鉴定及功能分析
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邱一洁, 齐飞扬, 杜何为, 黄敏*, * , 李曼菲*, *
作者信息
  • 长江大学生命科学学院,湖北 荆州 434025
通讯作者:
Identification and Functional Analysis of the Rice Vein Development Gene OsLVD1
Yijie QIU, Feiyang QI, Hewei DU, Min HUANG , Manfei LI
Affiliations
  • College of Life Sciences, Yangtze University, Jingzhou 434025, China
  • Corresponding Author:
出版时间: 2026-07-10 doi: 10.16819/j.1001-7216.2026.251016
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【目的】

光合作用决定水稻干物质积累,叶绿素含量和叶脉密度与光合作用密切相关。本研究在水稻中鉴定调控叶绿素含量和叶脉密度的基因OsLVD1,并对其功能进行解析。

【方法】

通过玉米叶脉发育基因同源比对获得OsLVD1,并利用生物信息学分析其蛋白特性、系统发育关系、信号肽、跨膜结构域、保守结构域及亚细胞定位。通过CRISPR获得lvd1编辑株,以野生型ZH11为对照,测定叶绿素含量、叶脉密度、可溶性糖含量及株高、分蘖数、每穗粒数等主要农艺性状,并通过qRT-PCR分析相关基因的表达水平。

【结果】

生物信息学分析表明,OsLVD1编码WRKY家族转录因子,定位于细胞核。lvd1因移码突变导致蛋白提前终止。与ZH11相比,lvd1叶绿素a、叶绿素b和总叶绿素分别下降25.90%、30.75%和27.05%,剑叶小脉密度和泡状细胞数目增加。lvd1株高降低且生育期略延迟,但叶片可溶性糖、结实率、千粒重等均无显著差异。qRT-PCR分析表明,lvd1中叶绿素合成相关基因OsHEMA1和OsCHLI的表达显著下调,而叶绿素降解基因OsNYC1及叶脉发育相关基因OsPIN1b表达上调。组织表达分析显示,OsLVD1在叶片中高表达,且在拔节期达到峰值。

【结论】

OsLVD1功能缺失导致叶绿素含量降低、叶脉密度增加,可能通过增加叶脉密度和改变泡状细胞发育补偿光合产物运输与水分保持,从而维持产量稳定。本研究揭示OsLVD1在叶片色素代谢与维管系统发育中的新功能,为光合结构优化提供理论基础。

水稻  /  OsLVD1  /  叶绿素含量  /  叶脉密度  /  WRKY转录因子  /  光合作用
【Objective】

Photosynthesis is a major determinant of dry matter accumulation in rice, with chlorophyll content and vein density being critical for photosynthetic efficiency. Here, a rice gene regulating chlorophyll content and vein density, OsLVD1, was identified and functionally analyzed.

【Method】

OsLVD1 was identified based on homology to maize vein development genes, and its protein features, phylogeny, conserved motifs, and subcellular localization were analyzed bioinformatically. An lvd1 mutant was generated using CRISPR/Cas9, with ZH11 as the wild type. Chlorophyll content, vein density, soluble sugar content, and major agronomic traits were examined. The expression of genes related to chlorophyll metabolism and vein development was analyzed by qRT-PCR.

【Result】

OsLVD1 encodes a nuclear-localized WRKY transcription factor, and the lvd1 mutation caused a frameshift leading to premature termination. Compared with ZH11, the lvd1 mutant showed reductions of 25.90%, 30.75%, and 27.05% in chlorophyll a, chlorophyll b, and total chlorophyll content, respectively, accompanied by increases in minor vein density and bulliform cell number. Plant height was reduced and growth duration was slightly delayed, whereas soluble sugar content, seed-setting rate, and thousand-grain weight were unaffected. qRT-PCR showed that the expression levels of chlorophyll biosynthesis genes OsHEMA1 and OsCHLI were downregulated, while those of the chlorophyll degradation gene OsNYC1 and the vein development gene OsPIN1b were upregulated in the lvd1 mutant. OsLVD1 was predominantly expressed in leaves, with the highest expression at the jointing stage.

【Conclusion】

Loss of OsLVD1 resulted in a "low chlorophyll-high vein density" phenotype, which may compensate for reduced photosynthesis by enhancing vein-mediated transport and water retention, thereby maintaining yield stability. This study demonstrates a novel role of OsLVD1 in coordinating leaf pigment metabolism and vascular development in rice.

Oryza sativa  /  OsLVD1  /  chlorophyll content  /  leaf vein density  /  WRKY transcription factor  /  photosynthesis
邱一洁, 齐飞扬, 杜何为, 黄敏, 李曼菲. 水稻叶脉基因OsLVD1的鉴定及功能分析. 中国水稻科学, 2026 , 40 (4) : 447 -459 . DOI: 10.16819/j.1001-7216.2026.251016
Yijie QIU, Feiyang QI, Hewei DU, Min HUANG, Manfei LI. Identification and Functional Analysis of the Rice Vein Development Gene OsLVD1[J]. Chinese Journal of Rice Science, 2026 , 40 (4) : 447 -459 . DOI: 10.16819/j.1001-7216.2026.251016
  • “十四五”国家重点研发计划资助项目(2025YFF1000200)
  • 湖北洪山实验室重大项目(2022hszd029)
  • 湖北省农业微生物产业发展重大专项(NYWSWZX2025-2027-13)
  • 国家自然科学基金青年项目(32301910; Yz2025268)
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文章信息
doi: 10.16819/j.1001-7216.2026.251016
  • 接收时间:2025-10-29
  • 首发时间:2026-09-17
  • 出版时间:2026-07-10
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出版历史
  • 收稿日期:2025-10-29
  • 修回日期:2026-01-26
基金
“十四五”国家重点研发计划资助项目(2025YFF1000200)
湖北洪山实验室重大项目(2022hszd029)
湖北省农业微生物产业发展重大专项(NYWSWZX2025-2027-13)
国家自然科学基金青年项目(32301910; Yz2025268)
作者信息
    长江大学生命科学学院,湖北 荆州 434025

通讯作者:

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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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