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In this study, fungal diversity was analyzed and antimicrobial activity isolates were screened from Eling Lake to enrich the fungi diversity in the extreme environment and to provide basic materials for subsequent development of biocontrol agents. Ten sediment samples were collected from shoreside of Eling Lake, and fungi were isolated by dilution separation method using seven selective mediums. The isolated fungi were identified by morphological characteristics and rDNA-ITS sequence phylogenetic analysis, and antimicrobial activity isolates were screened by confront culture method. One hundred and forty-four fungi isolates were obtained, belonging to totally 22 genera of Ascomycota, Mucoromycota and Zygomycota. Seven high antimicrobial activity isolates were screened from one hundred and forty-four fungi isolates, they had a broad spectrum of antimicrobial activity on eight fungi pathogens from maize, highland barley and wheat. Moreover, they were Phaeosphaeria and Emericellopsis, respectively. These results showed that sediment fungi resource in Eling Lake was abundant and have certain biocontrol and utilization value.

, authors=He-xing QI*, Cheng-wei XIN, Run-yan YANG, Kai-fu ZHENG, authorsList=He-xing QI, Cheng-wei XIN, Run-yan YANG, Kai-fu ZHENG, authorCompany=null, correspAuthors=He-xing QI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1315696655958135383, articleId=1315696655488373333, tenantId=1146029695717560320, journalId=1315121759093096577, language=CN, title=黄河源区鄂陵湖真菌多样性及抑菌活性菌株的筛选, columnId=1315696197449405326, journalTitle=微生物学杂志, columnName=研究报告, runingTitle=null, highlight=null, articleAbstract=

为解析鄂陵湖真菌多样性并筛选抑菌活性菌株,探究高寒极端湖泊湿地真菌多样性,从黄河源区鄂陵湖岸边采集底泥样本10份,使用7种选择性培养基并采用稀释涂板法分离和纯化真菌,基于ITS基因序列构建系统发育树并结合形态学特征鉴定真菌,使用对峙培养法筛选抑菌活性菌株,共分离得到144株真菌,分别隶属于子囊菌门(Ascomycota)、毛霉门(Mucoromycota)和接合菌门(Zygomycota)的22个属。并从中筛选得到7株对玉米、青稞和小麦来源的8种病原真菌具有广谱抑菌活性的菌株,分别隶属于暗球腔菌属(Phaeosphaeria)和翅孢壳属(Emericellopsis)。结果表明,鄂陵湖底泥真菌资源丰富,具有一定的生物防治利用价值。

, authors=祁鹤兴*, 辛承伟, 杨闰艳, 郑开福, authorsList=祁鹤兴, 辛承伟, 杨闰艳, 郑开福, authorCompany=null, correspAuthors=祁鹤兴, authorNote=

祁鹤兴 女,副教授,博士,硕士生导师。主要研究方向为植物病原真菌及病害生物防治。E-mail:

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* 通讯作者。
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祁鹤兴 女,副教授,博士,硕士生导师。主要研究方向为植物病原真菌及病害生物防治。E-mail:

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祁鹤兴 女,副教授,博士,硕士生导师。主要研究方向为植物病原真菌及病害生物防治。E-mail:

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黄河源区鄂陵湖真菌多样性及抑菌活性菌株的筛选
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祁鹤兴 * , 辛承伟 , 杨闰艳 , 郑开福
微生物学杂志 | 研究报告 2026,46(1): 24-32
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微生物学杂志 |研究报告 2026 , 46 (1) : 24 -32
黄河源区鄂陵湖真菌多样性及抑菌活性菌株的筛选
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祁鹤兴* , 辛承伟, 杨闰艳, 郑开福
作者信息
  • 青海大学 农牧学院,青海 西宁 810016
通讯作者:
* 通讯作者。
作者简介:

祁鹤兴 女,副教授,博士,硕士生导师。主要研究方向为植物病原真菌及病害生物防治。E-mail:

Fungi Diversity of Eling Lake in the Source Region of the Yellow River and Screening of Antimicrobial Activity Strains
He-xing QI* , Cheng-wei XIN, Run-yan YANG, Kai-fu ZHENG
Affiliations
  • College of Agriculture and Animal Husbandry, Qinghai University, Xining 810016
出版时间: 2026-02-20 doi: 10.3969/j.issn.1005-7021.2026.01.003
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为解析鄂陵湖真菌多样性并筛选抑菌活性菌株,探究高寒极端湖泊湿地真菌多样性,从黄河源区鄂陵湖岸边采集底泥样本10份,使用7种选择性培养基并采用稀释涂板法分离和纯化真菌,基于ITS基因序列构建系统发育树并结合形态学特征鉴定真菌,使用对峙培养法筛选抑菌活性菌株,共分离得到144株真菌,分别隶属于子囊菌门(Ascomycota)、毛霉门(Mucoromycota)和接合菌门(Zygomycota)的22个属。并从中筛选得到7株对玉米、青稞和小麦来源的8种病原真菌具有广谱抑菌活性的菌株,分别隶属于暗球腔菌属(Phaeosphaeria)和翅孢壳属(Emericellopsis)。结果表明,鄂陵湖底泥真菌资源丰富,具有一定的生物防治利用价值。

黄河源区  /  鄂陵湖  /  湿地真菌  /  形态学  /  多样性  /  抑菌活性菌株

In this study, fungal diversity was analyzed and antimicrobial activity isolates were screened from Eling Lake to enrich the fungi diversity in the extreme environment and to provide basic materials for subsequent development of biocontrol agents. Ten sediment samples were collected from shoreside of Eling Lake, and fungi were isolated by dilution separation method using seven selective mediums. The isolated fungi were identified by morphological characteristics and rDNA-ITS sequence phylogenetic analysis, and antimicrobial activity isolates were screened by confront culture method. One hundred and forty-four fungi isolates were obtained, belonging to totally 22 genera of Ascomycota, Mucoromycota and Zygomycota. Seven high antimicrobial activity isolates were screened from one hundred and forty-four fungi isolates, they had a broad spectrum of antimicrobial activity on eight fungi pathogens from maize, highland barley and wheat. Moreover, they were Phaeosphaeria and Emericellopsis, respectively. These results showed that sediment fungi resource in Eling Lake was abundant and have certain biocontrol and utilization value.

the Source Region of the Yellow River  /  Eling Lake  /  wetland fungi  /  morphology  /  fungi diversity  /  antimicrobial activity strains
祁鹤兴, 辛承伟, 杨闰艳, 郑开福. 黄河源区鄂陵湖真菌多样性及抑菌活性菌株的筛选. 微生物学杂志, 2026 , 46 (1) : 24 -32 . DOI: 10.3969/j.issn.1005-7021.2026.01.003
He-xing QI, Cheng-wei XIN, Run-yan YANG, Kai-fu ZHENG. Fungi Diversity of Eling Lake in the Source Region of the Yellow River and Screening of Antimicrobial Activity Strains[J]. Journal of Microbiology, 2026 , 46 (1) : 24 -32 . DOI: 10.3969/j.issn.1005-7021.2026.01.003
  • 青海省自然科学基金项目(2022-ZJ-977Q)
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doi: 10.3969/j.issn.1005-7021.2026.01.003
  • 接收时间:2025-05-09
  • 首发时间:2026-10-10
  • 出版时间:2026-02-20
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  • 收稿日期:2025-05-09
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青海省自然科学基金项目(2022-ZJ-977Q)
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    青海大学 农牧学院,青海 西宁 810016

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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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