微生物学杂志

不同地点手掌参内生细菌群落多样性分析

PDF下载

尹秀 ¹^,²^,³ , 张二豪 ¹^,²^,³ , 禄亚洲 ¹^,²^,³^,⁴ , 兰小中 ¹^,²^,³^,⁴^,^*

微生物学杂志 | 研究报告 2026,46(1): 44-55

收起

微生物学杂志 |研究报告 2026 , 46 (1) : 44 -55

不同地点手掌参内生细菌群落多样性分析

全屏

尹秀¹^,²^,³, 张二豪¹^,²^,³, 禄亚洲¹^,²^,³^,⁴, 兰小中¹^,²^,³^,⁴^,^*

作者信息

^1．西藏农牧大学食品科学学院，西藏林芝　860000

^2．西藏农牧大学西藏自治区藏药资源保护与利用重点实验室，西藏林芝　860000

^3．西藏农牧大学西藏特色农牧资源研发省部共建协同创新中心，西藏林芝　860000

^4．西藏农牧学院西藏中（藏）药资源中心，西藏林芝　860000

通讯作者:

^* 通讯作者。男，教授，博士，博士生导师。主要从事药用植物分子生物学研究。E-mail: lanxiaozhong@163.com

作者简介:

尹秀　女，讲师，硕士。主要从事药用植物分子生物学研究。E-mail: 624146260@qq.com

Analysis on the Community Diversity of Endophytic Bacteria in Gymnadenia conopsea at Different Locations

Xiu YIN¹^,²^,³, Er-hao ZHANG¹^,²^,³, Ya-zhou LU¹^,²^,³^,⁴, Xiao-zhong LAN¹^,²^,³^,⁴^,^*

Affiliations

^1.Food Science College, Xizang Agriculture and Animal Husbandry University, Nyingchi 860000

^2.Key Laboratory of Xizang Medicine Resources Conservation and Utilization of Xizang Autonomous Region, Xizang Agriculture and Animal Husbandry University, Nyingchi 860000

^3.The Provincial and Ministerial Co-founded Collaborative Innovation Center for R&D in Xizang Characteristic Agricultural and Animal Husbandry Resources, Xizang Agriculture and Animal Husbandry University, Nyingchi 860000

^4.The Center for Xizang Chinese (Tibetan) Medicine Resource, Xizang Agriculture and Animal Husbandry University, Nyingchi 860000

出版时间: 2026-02-20 doi: 10.3969/j.issn.1005-7021.2026.01.005

文章导航

摘要

收起

以西藏自治区林芝市色季拉山4个采样点手掌参（Gymnadenia conopsea）为材料，利用高通量测序技术对其16S rRNA进行测序，以探索不同环境因子对手掌参内生细菌群落多样性的影响。结果表明，从所有手掌参样品中共获得715 920条有效序列，聚类为2 365个OTUs，隶属于5门38科62属。优势菌门为变形菌门（Proteobacteria），优势菌属为假单胞菌属（Pseudomonas）、甲基杆菌属（Methylobacterium）和鞘脂单胞菌属（Sphingomonas）。多样性分析表明所有样品中林海观景台（LH）采样点手掌参茎样品（LHS）内生细菌群落丰富度最高，雷达站（LD）采样点手掌参果荚样品（LDF）内生细菌群落丰富度最低；废弃木材厂（FM）采样点手掌参茎样品（FMS）内生细菌群落多样性最丰富，该采样点手掌参的茎、叶和果荚细菌群落多样性指数均高于其他3个采样点。四种组织样品中，茎、叶和根中内生细菌群落丰富度均高于果荚，果荚中内生细菌群落多样性和丰富度均随海拔升高而降低，雷达站（LD）采样点手掌参果荚样品（LDF）中内生细菌群落丰富度和多样性均最低。不同生境下相同组织内生细菌群落多样性具有显著差异性，相同生境下不同组织内生细菌群落多样性也存在一定差异。主坐标分析（Principal Coordinates Analysis, PCoA）表明内生细菌的群落组成受到环境因素和组织类型的影响，同时冗余分析（redundancy analysis, RDA）和相关性热图均表明全磷（TP）、全钾（TK）、pH和海拔为主要影响因子，与内生细菌群落组成存在显著相关性。PICRSt功能预测分析表明手掌参内生细菌主要富集在新陈代谢（Metabolism）、环境信息处理（Environmental information processing）、遗传信息处理（Genetic information processing）三个一级代谢通路，大多代谢通路随着海拔高度升高呈下降趋势。本研究采用高通量测序技术分析环境因子和组织差异对手掌参内生细菌的多样性和群落结构的影响，旨在丰富和完善野生手掌参内生细菌资源，为今后有益内生细菌的资源开发及手掌参野生资源保护提供参考。

关键词

手掌参 / 内生细菌 / 多样性 / 细菌群落结构 / 环境因子

Abstract

收起

In this study, we performed 16S rRNA high-throughput sequencing to examine the diversity of endophytic bacterial communities in Gymnadenia conopsea growing at four different locations on Mount Sejila. The results revealed that a total of 715 920 effective sequences were obtained from all samples, which were associated with 2 365 operational taxonomic units, classified within five phyla, 38 families, and 62 genera. Proteobacteria was identified as the dominant bacterial phylum and the predominant genera were Pseudomonas, Methylbacterium, and Sphingomonas. Diversity analysis revealed that among the collected samples, the stem samples（LHS）collected at the Linhai Observation Deck were characterized by the highest endophytic bacterial community richness, whereas the richness of fruit samples（LDF）collected from the Radar Station was the lowest. The stem samples（FMS）from the Abandoned Wood Factory site were found to have the highest diversity of endophytic bacterial communities, and the diversity indices of communities in the stems, leaves, and fruits of plants growing at this sampling point（FM）at a lower altitude were higher than those recorded at the other three sampling points. Among the four types of tissue collected, the richness of endophytic bacterial communities in the stems, leaves, and roots was higher than that in the fruits, and we found that the diversity and richness of endophytic bacterial communities in fruits declined with increasing altitude, with the values obtained for the endophytic bacteria in fruit pods（LDF）being the lowest. Furthermore, we detected significant differences in the diversity of endophytic bacterial communities in the same tissue in different habitats, whereas there were differences in the diversity of endophytic bacteria within the different tissues of plants growing in the same habitat. Principal coordinate analysis indicated that the composition of endophytic bacterial communities was influenced by environmental factors and tissue types, and redundancy analysis and correlation heatmaps provided evidence to indicate that total phosphorus, total potassium, pH, and altitude were the main influencing factors, significantly correlated with the composition of these endophytic bacterial communities. Moreover, PICRSt prediction analysis revealed that the endophytic bacteria in G. conopsea were mainly enriched in three primary metabolic pathways, namely, metabolism, environmental information processing, and genetic information processing, and the activity of most of the identified metabolic pathways was found to show a declining trend with increasing altitude. This study employed high-throughput sequencing technology to analyze the effects of environmental factors and tissue types on the diversity and community structure of endophytic bacteria in G. conopsea. It aims to enrich and improve the resources of endophytic bacteria in wild G. conopsea, laying a foundation for the future resource development of beneficial endophytic bacteria and the protection of wild G. conopsea.

Key words

Gymnadenia conopsea / endophytic bacteria / diversity / bacterial community structure / environmental factors

引用本文

尹秀, 张二豪, 禄亚洲, 兰小中. 不同地点手掌参内生细菌群落多样性分析. 微生物学杂志, 2026 , 46 (1) : 44 -55 . DOI: 10.3969/j.issn.1005-7021.2026.01.005

Xiu YIN, Er-hao ZHANG, Ya-zhou LU, Xiao-zhong LAN. Analysis on the Community Diversity of Endophytic Bacteria in Gymnadenia conopsea at Different Locations[J]. Journal of Microbiology, 2026 , 46 (1) : 44 -55 . DOI: 10.3969/j.issn.1005-7021.2026.01.005

基金

收起

第四次全国中药资源普查西藏自治区中（藏）药资源普查项目(20200501-542301)
生物技术一流专业建设项目(2024-007)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

2026年第46卷第1期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.3969/j.issn.1005-7021.2026.01.005

接收时间：2025-11-09
首发时间：2026-10-10
出版时间：2026-02-20

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-11-09

基金

第四次全国中药资源普查西藏自治区中（藏）药资源普查项目(20200501-542301)

生物技术一流专业建设项目(2024-007)

作者信息

^1．西藏农牧大学食品科学学院，西藏林芝　860000

^2．西藏农牧大学西藏自治区藏药资源保护与利用重点实验室，西藏林芝　860000

^3．西藏农牧大学西藏特色农牧资源研发省部共建协同创新中心，西藏林芝　860000

^4．西藏农牧学院西藏中（藏）药资源中心，西藏林芝　860000

通讯作者:

^* 通讯作者。男，教授，博士，博士生导师。主要从事药用植物分子生物学研究。E-mail: lanxiaozhong@163.com

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/wswxzz/CN/10.3969/j.issn.1005-7021.2026.01.005

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT