Article(id=1315696650224521754, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696649406624175, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1005-7021.2026.01.004, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1759680000000, receivedDateStr=2025-10-06, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791616125875, onlineDateStr=2026-10-10, pubDate=1771516800000, pubDateStr=2026-02-20, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791616125875, onlineIssueDateStr=2026-10-10, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791616125875, creator=13701087609, updateTime=1791616125875, updator=13701087609, issue=Issue{id=1315696649406624175, tenantId=1146029695717560320, journalId=1315121759093096577, year='2026', volume='46', issue='1', pageStart='1', pageEnd='136', issueExtLink='null', onlineDate='null', pubDate='1771516800000', pubDateStr='2026-02-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1791616125680, creator='13701087609', updateTime=1791616242659, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1315697140115026638, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696649406624175, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1315697140115026639, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696649406624175, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=33, endPage=43, ext={EN=ArticleExt(id=1315696650564260379, articleId=1315696650224521754, tenantId=1146029695717560320, journalId=1315121759093096577, language=EN, title=Isolation and Identification of Heterotrophic Nitrifying Bacteria from Cow Manure and Their Nitrification Capacity, columnId=null, journalTitle=Journal of Microbiology, columnName=null, runingTitle=null, highlight=null, articleAbstract=

To reduce nitrogen loss during aerobic composting, enhance compost fertility, and minimize environmental pollution, this study used cattle manure as the material to isolate a highly efficient heterotrophic nitrifying strain of NB-2. Through morphological characterization and 16S rRNA gene sequence analysis, the strain was identified as Alcaligenes faecalis. Further research showed that NB-2 exhibited significantly enhanced ammonia oxidation capacity under the conditions of sodium citrate as the carbon source, C/N ratio of 15, temperature of 35 ℃, and pH of 9.0. The ammonia nitrogen () removal rate reached 100% at 32 hours, with a removal rate as high as 33.38 mg/(L·h). This study demonstrated the potential of strain NB-2 in composting applications to reduce nitrogen losses while improving fertilizer quality and efficiency, thereby promoting the sustainable development of the livestock industry.

, authors=Jia-li WANG, Zi-yu WANG, Yong-qi MA, Yun-peng JIANG, Liang-jia AN, Xiang-min HAN, Li-kun SUN*, authorsList=Jia-li WANG, Zi-yu WANG, Yong-qi MA, Yun-peng JIANG, Liang-jia AN, Xiang-min HAN, Li-kun SUN, authorCompany=null, correspAuthors=Li-kun SUN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1315696651499590176, articleId=1315696650224521754, tenantId=1146029695717560320, journalId=1315121759093096577, language=CN, title=牛粪中异养硝化细菌的分离鉴定及其硝化能力的研究, columnId=1315696197449405326, journalTitle=微生物学杂志, columnName=研究报告, runingTitle=null, highlight=null, articleAbstract=

为降低好氧堆肥氮素的损失,提高堆肥肥力,减少环境污染,以牛粪为主要材料,筛选出一株高效异养硝化菌株NB-2,并通过形态学特征及16S rRNA基因序列分析,确认其为粪产碱杆菌(Alcaligenes faecalis)。进一步研究显示,在以柠檬酸三钠为碳源、C/N比为15、温度为35 ℃以及pH值为9.0的条件下,菌株NB-2的硝化能力显著提升,在好氧发酵32 h时,菌株NB-2的氨氮()去除率为100%,去除速率高达33.38 mg/(L·h)。本研究结果表明,菌株NB-2应用于堆肥具有减少氮素损失的潜力,同时能提高肥料的质量和效率,从而促进畜牧业的可持续发展。

, authors=王佳丽, 王梓宇, 马咏琪, 姜云鹏, 安亮嘉, 韩向敏, 孙丽坤*, authorsList=王佳丽, 王梓宇, 马咏琪, 姜云鹏, 安亮嘉, 韩向敏, 孙丽坤, authorCompany=null, correspAuthors=孙丽坤, authorNote=

王佳丽与王梓宇为并列第一作者。

王佳丽 女,硕士研究生。主要研究方向为畜牧微生物。E-mail:

王梓宇 男,硕士研究生。主要研究方向为畜牧微生物。E-mail:

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* 通讯作者。女,副教授,博士。主要研究方向为畜牧环境微生物。E-mail:
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牛粪中异养硝化细菌的分离鉴定及其硝化能力的研究
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王佳丽 , 王梓宇 , 马咏琪 , 姜云鹏 , 安亮嘉 , 韩向敏 , 孙丽坤 *
微生物学杂志 | 研究报告 2026,46(1): 33-43
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微生物学杂志 |研究报告 2026 , 46 (1) : 33 -43
牛粪中异养硝化细菌的分离鉴定及其硝化能力的研究
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王佳丽 , 王梓宇 , 马咏琪, 姜云鹏, 安亮嘉, 韩向敏, 孙丽坤*
作者信息
  • 甘肃农业大学 动物科学技术学院,甘肃 兰州 730070
通讯作者:
* 通讯作者。女,副教授,博士。主要研究方向为畜牧环境微生物。E-mail:
作者简介:

王佳丽与王梓宇为并列第一作者。

王佳丽 女,硕士研究生。主要研究方向为畜牧微生物。E-mail:

王梓宇 男,硕士研究生。主要研究方向为畜牧微生物。E-mail:

Isolation and Identification of Heterotrophic Nitrifying Bacteria from Cow Manure and Their Nitrification Capacity
Jia-li WANG , Zi-yu WANG , Yong-qi MA, Yun-peng JIANG, Liang-jia AN, Xiang-min HAN, Li-kun SUN*
Affiliations
  • College of Animal Science and Technology, Gansu Agricultural University, Lanzhou 730070
出版时间: 2026-02-20 doi: 10.3969/j.issn.1005-7021.2026.01.004
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为降低好氧堆肥氮素的损失,提高堆肥肥力,减少环境污染,以牛粪为主要材料,筛选出一株高效异养硝化菌株NB-2,并通过形态学特征及16S rRNA基因序列分析,确认其为粪产碱杆菌(Alcaligenes faecalis)。进一步研究显示,在以柠檬酸三钠为碳源、C/N比为15、温度为35 ℃以及pH值为9.0的条件下,菌株NB-2的硝化能力显著提升,在好氧发酵32 h时,菌株NB-2的氨氮()去除率为100%,去除速率高达33.38 mg/(L·h)。本研究结果表明,菌株NB-2应用于堆肥具有减少氮素损失的潜力,同时能提高肥料的质量和效率,从而促进畜牧业的可持续发展。

硝化细菌  /  氮素转化  /  异养硝化  /  粪产碱杆菌(Alcaligenes faecalis)  /  硝化特性

To reduce nitrogen loss during aerobic composting, enhance compost fertility, and minimize environmental pollution, this study used cattle manure as the material to isolate a highly efficient heterotrophic nitrifying strain of NB-2. Through morphological characterization and 16S rRNA gene sequence analysis, the strain was identified as Alcaligenes faecalis. Further research showed that NB-2 exhibited significantly enhanced ammonia oxidation capacity under the conditions of sodium citrate as the carbon source, C/N ratio of 15, temperature of 35 ℃, and pH of 9.0. The ammonia nitrogen () removal rate reached 100% at 32 hours, with a removal rate as high as 33.38 mg/(L·h). This study demonstrated the potential of strain NB-2 in composting applications to reduce nitrogen losses while improving fertilizer quality and efficiency, thereby promoting the sustainable development of the livestock industry.

nitrifying bacteria  /  nitrogen transformation  /  heterotrophic nitrification  /  Alcaligenes faecalis  /  nitrification characteristics
王佳丽, 王梓宇, 马咏琪, 姜云鹏, 安亮嘉, 韩向敏, 孙丽坤. 牛粪中异养硝化细菌的分离鉴定及其硝化能力的研究. 微生物学杂志, 2026 , 46 (1) : 33 -43 . DOI: 10.3969/j.issn.1005-7021.2026.01.004
Jia-li WANG, Zi-yu WANG, Yong-qi MA, Yun-peng JIANG, Liang-jia AN, Xiang-min HAN, Li-kun SUN. Isolation and Identification of Heterotrophic Nitrifying Bacteria from Cow Manure and Their Nitrification Capacity[J]. Journal of Microbiology, 2026 , 46 (1) : 33 -43 . DOI: 10.3969/j.issn.1005-7021.2026.01.004
  • 国家自然科学基金项目(32160756; 32260354)
  • 甘肃省自然科学基金计划项目(24JRRA644)
  • 甘肃农业大学“伏羲杰出人才培育计划”项目(GAUfx-04J03)
  • 甘肃生猪产业配套技术研发与应用项目(GNKJ-2024-46)
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2026年第46卷第1期
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doi: 10.3969/j.issn.1005-7021.2026.01.004
  • 接收时间:2025-10-06
  • 首发时间:2026-10-10
  • 出版时间:2026-02-20
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  • 收稿日期:2025-10-06
基金
国家自然科学基金项目(32160756; 32260354)
甘肃省自然科学基金计划项目(24JRRA644)
甘肃农业大学“伏羲杰出人才培育计划”项目(GAUfx-04J03)
甘肃生猪产业配套技术研发与应用项目(GNKJ-2024-46)
作者信息
    甘肃农业大学 动物科学技术学院,甘肃 兰州 730070

通讯作者:

* 通讯作者。女,副教授,博士。主要研究方向为畜牧环境微生物。E-mail:
参考文献
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https://castjournals.cast.org.cn/joweb/wswxzz/CN/10.3969/j.issn.1005-7021.2026.01.004
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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