Article(id=1315696197176775564, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696195121558078, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1005-7021.2026.04.004, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1765123200000, receivedDateStr=2025-12-08, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791616017860, onlineDateStr=2026-10-10, pubDate=1787155200000, pubDateStr=2026-08-20, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791616017860, onlineIssueDateStr=2026-10-10, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791616017860, creator=13701087609, updateTime=1791616017860, updator=13701087609, issue=Issue{id=1315696195121558078, tenantId=1146029695717560320, journalId=1315121759093096577, year='2026', volume='46', issue='4', pageStart='1', pageEnd='144', issueExtLink='null', onlineDate='null', pubDate='1787155200000', pubDateStr='2026-08-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1791616017369, creator='13701087609', updateTime=1791616161314, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1315696798950339227, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696195121558078, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1315696798950339228, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696195121558078, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=42, endPage=53, ext={EN=ArticleExt(id=1315696197340353421, articleId=1315696197176775564, tenantId=1146029695717560320, journalId=1315121759093096577, language=EN, title=In Situ Enrichment of Actinoplanes Using Polyaspartic Acid Water Retaining Agent and Screening of Its Secondary Metabolic Potential, columnId=null, journalTitle=Journal of Microbiology, columnName=null, runingTitle=null, highlight=null, articleAbstract=

The Tarim River Basin is rich in microbial resources. To explore the species diversity of Actinoplanes in its soil, uncover new Actinoplanes resources, and enrich the germplasm resource bank of the Tarim River Basin, the in situ enrichment of Actinoplanes was achieved using polyaspartic acid water-retaining agent (PASP). The fermentation crude extracts of Actinoplanes were screened for antimicrobial activity using seven pathogenic bacteria as target strains. This was combined with antiSMASH gene cluster analysis and detection of antibiotic biosynthesis genes. The results indicate that 244 strains isolated and identified from soil samples enriched in situ with PASP belong to 3 phyla, 5 classes, 4 orders, 11 families, 16 genera, and 59 species, respectively. Among these, 228 strains belong to the phylum Actinomycetota, accounting for 93%. The genus Actinoplanes exhibited a high isolation rate, with 103 isolated strains and 21 species, representing 42%. Seven strains of Actinoplanes with both antimicrobial activity and metabolic potential were screened. Soil samples with high moisture content along the Tarim River harbor abundant rare actinobacterial resources. The PASP in situ enrichment method can selectively isolate Actinoplanes, thereby facilitating further research on the diversity of rare actinobacteria in the soil microorganisms of the Tarim River Basin and on the exploration and utilization of Actinoplanes resources.

, authors=Pei-zhi DING^{1, 2}, Hong-ling SHEN^{1, 2}, Qing-tang DAI¹, Han GAO¹, Xing-xin HUANG¹, Chuan-xing WAN^{1, 2, *}, authorsList=Pei-zhi DING, Hong-ling SHEN, Qing-tang DAI, Han GAO, Xing-xin HUANG, Chuan-xing WAN, authorCompany=null, correspAuthors=Chuan-xing WAN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1315696197780755343, articleId=1315696197176775564, tenantId=1146029695717560320, journalId=1315121759093096577, language=CN, title=聚天冬氨酸保水剂原位富集游动放线菌及其次级代谢潜力筛选, columnId=1315696197449405326, journalTitle=微生物学杂志, columnName=研究报告, runingTitle=null, highlight=null, articleAbstract=

塔里木河流域具有丰富的微生物资源，为了探索该区域土壤中游动放线菌属（Actinoplans）的物种多样性，挖掘新的游动放线菌资源，丰富塔里木河流域种质资源库，通过聚天冬氨酸（polyaspartic acid, PASP）保水剂实现游动放线菌的原位富集，采用7种病原菌作为靶标菌对游动放线菌的发酵粗提物进行抗菌活性筛选，结合antiSMASH基因簇分析并检测抗生素生物合成基因。结果表明，经PASP保水剂原位富集的土样中分离鉴定出244株菌株，分属于3门5纲4目11科16属59种，其中放线菌门（Actinomycetota）菌株228株，占93%，游动放线菌属的分离率高，分离得到103株，21种，占42%。筛选出7株兼具抗菌活性与代谢潜力的游动放线菌菌株，说明在塔里木河沿岸含水量高的土样中存在丰富的稀有放线菌资源，PASP原位富集方法可选择性地分离获得游动放线菌，这有利于进一步研究塔里木河流域土壤微生物中稀有放线菌的多样性，为开发利用游动放线菌资源提供参考。

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丁培治　女，博士研究生。研究方向为微生物资源挖掘与利用。E-mail: 1797487953@qq.com

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丁培治　女，博士研究生。研究方向为微生物资源挖掘与利用。E-mail: 1797487953@qq.com

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