Article(id=1315696205003338422, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696195121558078, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1005-7021.2026.04.003, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1764604800000, receivedDateStr=2025-12-02, revisedDate=null, revisedDateStr=null, acceptedDate=null, acceptedDateStr=null, onlineDate=1791616019725, onlineDateStr=2026-10-10, pubDate=1787155200000, pubDateStr=2026-08-20, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1791616019725, onlineIssueDateStr=2026-10-10, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1791616019725, creator=13701087609, updateTime=1791616019725, updator=13701087609, issue=Issue{id=1315696195121558078, tenantId=1146029695717560320, journalId=1315121759093096577, year='2026', volume='46', issue='4', pageStart='1', pageEnd='144', issueExtLink='null', onlineDate='null', pubDate='1787155200000', pubDateStr='2026-08-20', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1791616017369, creator='13701087609', updateTime=1791616161314, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1315696798950339227, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696195121558078, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1315696798950339228, tenantId=1146029695717560320, journalId=1315121759093096577, issueId=1315696195121558078, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=28, endPage=41, ext={EN=ArticleExt(id=1315696205175304887, articleId=1315696205003338422, tenantId=1146029695717560320, journalId=1315121759093096577, language=EN, title=Genomic Characteristics and Fermentation Function Analysis on a Strain of Lactobacillus plantarum Employed in Silage Preparation, columnId=null, journalTitle=Journal of Microbiology, columnName=null, runingTitle=null, highlight=null, articleAbstract=

This study aims to investigate the effect ofdominant lactic acid bacteria (LAB) on the quality of silage feed and its genomic characteristics related to lignocellulose degradation and lactic acid metabolism. Four dominant strains of Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, Weissella cibaria, Pediococcus acidilacticiwere isolated from silage feed and then used as microbial additives for a 50-day silage fermentation. The results showed that Lactiplantibacillus plantarum ZR exhibited superior performance compared to other lactic acid bacteria, with better retention of dry matter content and higher cellulase activity. Additionally, harmful substances such as ADF, NDF, and ammonia nitrogen were significantly reduced compared to other treatment groups. The genomic analysis revealed that Lactiplantibacillus plantarum ZR is 3 398 411 bp in length, consisting of 1 chromosome and 6 plasmids, with a GC content of 44.38%. CAZyme analysis indicated that the genome of Lactiplantibacillus plantarum ZR contains 534 CAZyme genes, and 30 genes related to lactic acid synthesis. Comparative genomic analysis revealed the presence of insertions, deletions, and inversions in the genome of Lactiplantibacillus plantarum ZR, involving genes such as scrR, bglA, malL, galE, agrA, oppA, opuA and others. In conclusion, this study elucidates that Lactiplantibacillus plantarum ZR exhibiting robust silage fermentation capabilities. Additionally, it reveals the genomic characteristics of the strain Lactiplantibacillus plantarum ZR. These findings provide a theoretical basis for the application of this strain at an industrial level.

, authors=Yi-xin HAO1, Ping CHEN1, Yao-xing ZHANG1, Yu-wei ZHAO2, Yan-shun NIE3, Wen-xing HE1, Wen-bo WANG1, *, authorsList=Yi-xin HAO, Ping CHEN, Yao-xing ZHANG, Yu-wei ZHAO, Yan-shun NIE, Wen-xing HE, Wen-bo WANG, authorCompany=null, correspAuthors=Wen-bo WANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1315696205850587832, articleId=1315696205003338422, tenantId=1146029695717560320, journalId=1315121759093096577, language=CN, title=一株用于青贮饲料制备的植物乳杆菌基因组特征及发酵功能分析, columnId=1315696197449405326, journalTitle=微生物学杂志, columnName=研究报告, runingTitle=null, highlight=null, articleAbstract=

为解析优势乳酸菌(Lactic acid bacteria, LAB)对构叶青贮饲料品质的影响及与青贮发酵功能相关的基因组特征,从构叶青贮饲料中分离得到植物乳杆菌(Lactiplantibacillus plantarum)ZR、乳酸片球菌(Pediococcus acidilactici)RP、融合魏斯氏菌(Weissella cibaria)RW和副干酪乳杆菌(Lacticaseibacillus paracasei)R8四株优势菌株,作为菌种添加剂进行为期50 d的青贮发酵实验。结果发现Lactiplantibacillus plantarum ZR在构叶青贮饲料中干物质含量保持以及木质纤维素降解酶活性方面均优于其他菌株;酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)和氨态氮等有害物质含量较其他处理组有不同程度降低。全基因组测序分析结果表明,Lactiplantibacillus plantarum ZR基因组全长3 398 411 bp,包含1条染色体和6个质粒,GC含量为44.38%。CAZymes注释结果表明,Lactiplantibacillus plantarum ZR基因组包含534个CAZyme基因和30个乳酸合成相关基因。比较基因组分析发现,Lactiplantibacillus plantarum ZR基因组局部位置中存在插入、缺失和倒位等现象,涉及scrR、bglA、malL、galE、agrA、oppA、opuA等基因。本研究结果表明,Lactiplantibacillus plantarum ZR具有较强的青贮发酵功能,揭示了Lactiplantibacillus plantarum ZR的基因组特征,为该菌株的工业应用提供参考。

, authors=郝怡欣1, 陈萍1, 张耀星1, 赵煜炜2, 聂言顺3, 何文兴1, 王文波1, *, authorsList=郝怡欣, 陈萍, 张耀星, 赵煜炜, 聂言顺, 何文兴, 王文波, authorCompany=null, correspAuthors=王文波, authorNote=

郝怡欣 女,硕士研究生。研究方向为微生物学。E-mail:

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* 通讯作者。男,副教授,博士,硕士生导师。研究方向为微生物生态学。E-mail:
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一株用于青贮饲料制备的植物乳杆菌基因组特征及发酵功能分析
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郝怡欣 1 , 陈萍 1 , 张耀星 1 , 赵煜炜 2 , 聂言顺 3 , 何文兴 1 , 王文波 1, *
微生物学杂志 | 研究报告 2026,46(4): 28-41
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微生物学杂志 |研究报告 2026 , 46 (4) : 28 -41
一株用于青贮饲料制备的植物乳杆菌基因组特征及发酵功能分析
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郝怡欣 女,硕士研究生。研究方向为微生物学。E-mail:

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郝怡欣1 , 陈萍1, 张耀星1, 赵煜炜2, 聂言顺3, 何文兴1, 王文波1, *
作者信息
  • 1.济南大学 生物科学与技术学院,山东 济南 250022
  • 2.烟台龙大养殖有限公司,山东 烟台 265200
  • 3.丰唐生态农业技术研发(山东)有限公司,山东 泰安 271400
通讯作者:
* 通讯作者。男,副教授,博士,硕士生导师。研究方向为微生物生态学。E-mail:
作者简介:

郝怡欣 女,硕士研究生。研究方向为微生物学。E-mail:

Genomic Characteristics and Fermentation Function Analysis on a Strain of Lactobacillus plantarum Employed in Silage Preparation
Yi-xin HAO1 , Ping CHEN1, Yao-xing ZHANG1, Yu-wei ZHAO2, Yan-shun NIE3, Wen-xing HE1, Wen-bo WANG1, *
Affiliations
  • 1.School of Biological Science and Technology, University of Jinan, Jinan 250022
  • 2.Yantai Longda Breeding Co., Yantai 265200
  • 3.Fengtang ecological agriculture technology research and development (Shandong) Co., Taian 271400
出版时间: 2026-08-20 doi: 10.3969/j.issn.1005-7021.2026.04.003
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为解析优势乳酸菌(Lactic acid bacteria, LAB)对构叶青贮饲料品质的影响及与青贮发酵功能相关的基因组特征,从构叶青贮饲料中分离得到植物乳杆菌(Lactiplantibacillus plantarum)ZR、乳酸片球菌(Pediococcus acidilactici)RP、融合魏斯氏菌(Weissella cibaria)RW和副干酪乳杆菌(Lacticaseibacillus paracasei)R8四株优势菌株,作为菌种添加剂进行为期50 d的青贮发酵实验。结果发现Lactiplantibacillus plantarum ZR在构叶青贮饲料中干物质含量保持以及木质纤维素降解酶活性方面均优于其他菌株;酸性洗涤纤维(ADF)、中性洗涤纤维(NDF)和氨态氮等有害物质含量较其他处理组有不同程度降低。全基因组测序分析结果表明,Lactiplantibacillus plantarum ZR基因组全长3 398 411 bp,包含1条染色体和6个质粒,GC含量为44.38%。CAZymes注释结果表明,Lactiplantibacillus plantarum ZR基因组包含534个CAZyme基因和30个乳酸合成相关基因。比较基因组分析发现,Lactiplantibacillus plantarum ZR基因组局部位置中存在插入、缺失和倒位等现象,涉及scrR、bglA、malL、galE、agrA、oppA、opuA等基因。本研究结果表明,Lactiplantibacillus plantarum ZR具有较强的青贮发酵功能,揭示了Lactiplantibacillus plantarum ZR的基因组特征,为该菌株的工业应用提供参考。

乳酸菌  /  全基因组测序  /  构树青贮  /  乳酸合成途径  /  比较基因组

This study aims to investigate the effect ofdominant lactic acid bacteria (LAB) on the quality of silage feed and its genomic characteristics related to lignocellulose degradation and lactic acid metabolism. Four dominant strains of Lacticaseibacillus paracasei, Lactiplantibacillus plantarum, Weissella cibaria, Pediococcus acidilacticiwere isolated from silage feed and then used as microbial additives for a 50-day silage fermentation. The results showed that Lactiplantibacillus plantarum ZR exhibited superior performance compared to other lactic acid bacteria, with better retention of dry matter content and higher cellulase activity. Additionally, harmful substances such as ADF, NDF, and ammonia nitrogen were significantly reduced compared to other treatment groups. The genomic analysis revealed that Lactiplantibacillus plantarum ZR is 3 398 411 bp in length, consisting of 1 chromosome and 6 plasmids, with a GC content of 44.38%. CAZyme analysis indicated that the genome of Lactiplantibacillus plantarum ZR contains 534 CAZyme genes, and 30 genes related to lactic acid synthesis. Comparative genomic analysis revealed the presence of insertions, deletions, and inversions in the genome of Lactiplantibacillus plantarum ZR, involving genes such as scrR, bglA, malL, galE, agrA, oppA, opuA and others. In conclusion, this study elucidates that Lactiplantibacillus plantarum ZR exhibiting robust silage fermentation capabilities. Additionally, it reveals the genomic characteristics of the strain Lactiplantibacillus plantarum ZR. These findings provide a theoretical basis for the application of this strain at an industrial level.

lactic acid bacteria  /  genome-wide sequencing  /  Broussonetia papyrifera silage  /  lactic acid biosynthesis pathway  /  comparative genomics
郝怡欣, 陈萍, 张耀星, 赵煜炜, 聂言顺, 何文兴, 王文波. 一株用于青贮饲料制备的植物乳杆菌基因组特征及发酵功能分析. 微生物学杂志, 2026 , 46 (4) : 28 -41 . DOI: 10.3969/j.issn.1005-7021.2026.04.003
Yi-xin HAO, Ping CHEN, Yao-xing ZHANG, Yu-wei ZHAO, Yan-shun NIE, Wen-xing HE, Wen-bo WANG. Genomic Characteristics and Fermentation Function Analysis on a Strain of Lactobacillus plantarum Employed in Silage Preparation[J]. Journal of Microbiology, 2026 , 46 (4) : 28 -41 . DOI: 10.3969/j.issn.1005-7021.2026.04.003
  • 山东省重点研发计划项目(2023TZXD038)
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doi: 10.3969/j.issn.1005-7021.2026.04.003
  • 接收时间:2025-12-02
  • 首发时间:2026-10-10
  • 出版时间:2026-08-20
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  • 收稿日期:2025-12-02
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山东省重点研发计划项目(2023TZXD038)
作者信息
    1.济南大学 生物科学与技术学院,山东 济南 250022
    2.烟台龙大养殖有限公司,山东 烟台 265200
    3.丰唐生态农业技术研发(山东)有限公司,山东 泰安 271400

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* 通讯作者。男,副教授,博士,硕士生导师。研究方向为微生物生态学。E-mail:
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2种不同金属材料的力学参数
科
Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)
属
Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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