Article(id=1277255592171405429, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.03.014, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1760544000000, receivedDateStr=2025-10-16, revisedDate=null, revisedDateStr=null, acceptedDate=1764950400000, acceptedDateStr=2025-12-06, onlineDate=1782451063435, onlineDateStr=2026-06-26, pubDate=1774368000000, pubDateStr=2026-03-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782451063435, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782451063435, creator=13701087609, updateTime=1782451063435, updator=13701087609, issue=Issue{id=1277255461552390652, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='3', pageStart='553', pageEnd='823', issueExtLink='null', onlineDate='null', pubDate='1774368000000', pubDateStr='2026-03-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=1, specialIssue=null, createTime=1782451032293, creator='13701087609', updateTime=1782451557265, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277257664249852732, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277257664249852733, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277255461552390652, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=694, endPage=701, ext={EN=ArticleExt(id=1277255593761046647, articleId=1277255592171405429, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Establishment of a Nested PCR Detection Method for 16SrII Phytoplasma Associated with Areca Palm Yellow Leaf Disease, columnId=1236292524264968282, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Protection & Bio-safety, runingTitle=null, highlight=null, articleAbstract=

In China, areca palm yellow leaf disease (AYLD) is primarily attributed to infection with phytoplasmas from the 16SrI, 16SrII and 16SrXXXII groups. Currently, detection methods predominantly target the 16SrI group or non-specifically detect all three groups, lacking specific identification methods for the 16SrII group phytoplasmas. To achieve specific detection of 16SrII group phytoplasmas, specific nested PCR primers were designed and screened in this study based on the ribosomal protein (rp) gene sequence of 16SrII group phytoplasmas, and the characteristics and application efficacy of this method were subsequently evaluated. Results showed that the method could accurately distinguish positive samples from healthy ones. In the sensitivity comparison, the sensitivity of the method was higher than that of the universal nested PCR targeting the 16S rRNA and tuf genes. In the specificity evaluation, it showed no cross-reactivity with phytoplasmas from the 16SrI or 16SrXXXII groups, or with other tested areca palm pathogens and endophytic bacteria. In practical application, among 22 areca palm samples collected from Wenchang, Hainan Province, 4 samples were detected as positive; additionally, the method could detect 16SrII group phytoplasmas including Parthenium hysterophorus witches'-broom phytoplasma, Cleome rutidosperma witches'-broom phytoplasma, and peanut witches'-broom phytoplasma, demonstrating good applicability. In conclusion, the nested PCR method established in this study based on the rp gene has good specificity for the 16SrII group areca palm yellow leaf phytoplasmas, and could provide technical reference for field diagnosis and disease monitoring of the 16SrII group areca palm yellow leaf phytoplasmas.

, authors=null, authorsList=Zhaowei LIN, Qinghua TANG, Shaoshuai YU, Shida LONG, Weiwei SONG, authorCompany=null, correspAuthors=Weiwei SONG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277255594314694777, articleId=1277255592171405429, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=16SrII组槟榔黄化植原体巢式PCR检测方法的建立, columnId=1236292524520820846, journalTitle=热带作物学报, columnName=植物保护与生物安全, runingTitle=null, highlight=null, articleAbstract=

在中国,引起槟榔黄化病(areca palm yellow leaf disease, AYLD)的植原体主要有16SrI组、16SrII组及16SrXXXII组3个种群,但目前的检测技术主要针对16SrI组或3个种群的共同检测,缺乏对16SrII组植原体的特异性识别手段。为了对16SrII组槟榔黄化植原体进行特异性检测,本研究基于16SrII组植原体核糖体蛋白(rp)基因序列,设计并筛选特异性巢式PCR引物,并对该方法的特性及其应用效果进行评估。结果显示,在准确性方面,该方法能准确区分出阳性和阴性样品;在敏感性比较中,该方法敏感性高于16S rRNA和tuf基因的通用巢式PCR检测;在特异性评估中,16SrI组和16SrXXXII组植原体、供试的槟榔病原及内生菌均对本方法未造成干扰;在应用中,从海南文昌采集的22份槟榔样品检测出4份阳性样品,并且可检测出属于16SrII组的银胶菊丛枝植原体、皱子鸟足菜丛枝植原体及花生丛枝植原体,表现出较好的适用性。综上,本研究建立的基于rp基因的巢式PCR方法对16SrII组槟榔黄化植原体检测具有较好的特异性,可为16SrII组槟榔黄化植原体的田间诊断、病情监测提供技术参考。

, authors=

林兆威(1993—),男,硕士研究生,研究方向:热带经济作物主要病害综合防治。

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* 宋薇薇(SONG Weiwei),E-mail:
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林兆威(1993—),男,硕士研究生,研究方向:热带经济作物主要病害综合防治。

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林兆威(1993—),男,硕士研究生,研究方向:热带经济作物主要病害综合防治。

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16SrII组槟榔黄化植原体巢式PCR检测方法的建立
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林兆威 , 唐庆华 , 于少帅 , 龙仕达 , 宋薇薇 *, *
热带作物学报 | 植物保护与生物安全 2026,47(3): 694-701
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热带作物学报 |植物保护与生物安全 2026 , 47 (3) : 694 -701
16SrII组槟榔黄化植原体巢式PCR检测方法的建立
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林兆威, 唐庆华, 于少帅, 龙仕达, 宋薇薇*, *
作者信息
  • 中国热带农业科学院椰子研究所/海南省院士团队创新中心,海南文昌 571339
通讯作者:
* 宋薇薇(SONG Weiwei),E-mail:
Establishment of a Nested PCR Detection Method for 16SrII Phytoplasma Associated with Areca Palm Yellow Leaf Disease
Zhaowei LIN, Qinghua TANG, Shaoshuai YU, Shida LONG, Weiwei SONG
Affiliations
  • Coconut Research Institute, Chinese Academy of Tropical Agricultural Sciences/Hainan Innovation Center of Academician Team, Wenchang, Hainan 571339, China
出版时间: 2026-03-25 doi: 10.3969/j.issn.1000-2561.2026.03.014
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在中国,引起槟榔黄化病(areca palm yellow leaf disease, AYLD)的植原体主要有16SrI组、16SrII组及16SrXXXII组3个种群,但目前的检测技术主要针对16SrI组或3个种群的共同检测,缺乏对16SrII组植原体的特异性识别手段。为了对16SrII组槟榔黄化植原体进行特异性检测,本研究基于16SrII组植原体核糖体蛋白(rp)基因序列,设计并筛选特异性巢式PCR引物,并对该方法的特性及其应用效果进行评估。结果显示,在准确性方面,该方法能准确区分出阳性和阴性样品;在敏感性比较中,该方法敏感性高于16S rRNA和tuf基因的通用巢式PCR检测;在特异性评估中,16SrI组和16SrXXXII组植原体、供试的槟榔病原及内生菌均对本方法未造成干扰;在应用中,从海南文昌采集的22份槟榔样品检测出4份阳性样品,并且可检测出属于16SrII组的银胶菊丛枝植原体、皱子鸟足菜丛枝植原体及花生丛枝植原体,表现出较好的适用性。综上,本研究建立的基于rp基因的巢式PCR方法对16SrII组槟榔黄化植原体检测具有较好的特异性,可为16SrII组槟榔黄化植原体的田间诊断、病情监测提供技术参考。

槟榔  /  槟榔黄化病  /  植原体检测  /  巢式PCR

In China, areca palm yellow leaf disease (AYLD) is primarily attributed to infection with phytoplasmas from the 16SrI, 16SrII and 16SrXXXII groups. Currently, detection methods predominantly target the 16SrI group or non-specifically detect all three groups, lacking specific identification methods for the 16SrII group phytoplasmas. To achieve specific detection of 16SrII group phytoplasmas, specific nested PCR primers were designed and screened in this study based on the ribosomal protein (rp) gene sequence of 16SrII group phytoplasmas, and the characteristics and application efficacy of this method were subsequently evaluated. Results showed that the method could accurately distinguish positive samples from healthy ones. In the sensitivity comparison, the sensitivity of the method was higher than that of the universal nested PCR targeting the 16S rRNA and tuf genes. In the specificity evaluation, it showed no cross-reactivity with phytoplasmas from the 16SrI or 16SrXXXII groups, or with other tested areca palm pathogens and endophytic bacteria. In practical application, among 22 areca palm samples collected from Wenchang, Hainan Province, 4 samples were detected as positive; additionally, the method could detect 16SrII group phytoplasmas including Parthenium hysterophorus witches'-broom phytoplasma, Cleome rutidosperma witches'-broom phytoplasma, and peanut witches'-broom phytoplasma, demonstrating good applicability. In conclusion, the nested PCR method established in this study based on the rp gene has good specificity for the 16SrII group areca palm yellow leaf phytoplasmas, and could provide technical reference for field diagnosis and disease monitoring of the 16SrII group areca palm yellow leaf phytoplasmas.

areca  /  areca palm yellow leaf disease  /  phytoplasmas detection  /  nested PCR
林兆威, 唐庆华, 于少帅, 龙仕达, 宋薇薇. 16SrII组槟榔黄化植原体巢式PCR检测方法的建立. 热带作物学报, 2026 , 47 (3) : 694 -701 . DOI: 10.3969/j.issn.1000-2561.2026.03.014
Zhaowei LIN, Qinghua TANG, Shaoshuai YU, Shida LONG, Weiwei SONG. Establishment of a Nested PCR Detection Method for 16SrII Phytoplasma Associated with Areca Palm Yellow Leaf Disease[J]. Chinese Journal of Tropical Crops, 2026 , 47 (3) : 694 -701 . DOI: 10.3969/j.issn.1000-2561.2026.03.014
  • 中央级公益性科研院所基本科研业务费专项(1630152024006)
  • 农业农村部农作物病虫鼠害疫情监测与防治经费项目(102125211630150009005)
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doi: 10.3969/j.issn.1000-2561.2026.03.014
  • 接收时间:2025-10-16
  • 首发时间:2026-06-26
  • 出版时间:2026-03-25
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  • 收稿日期:2025-10-16
  • 录用日期:2025-12-06
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中央级公益性科研院所基本科研业务费专项(1630152024006)
农业农村部农作物病虫鼠害疫情监测与防治经费项目(102125211630150009005)
作者信息
    中国热带农业科学院椰子研究所/海南省院士团队创新中心,海南文昌 571339

通讯作者:

* 宋薇薇(SONG Weiwei),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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