Article(id=1277254285796708946, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.02.022, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1761408000000, receivedDateStr=2025-10-26, revisedDate=null, revisedDateStr=null, acceptedDate=1763568000000, acceptedDateStr=2025-11-20, onlineDate=1782450751970, onlineDateStr=2026-06-26, pubDate=1771948800000, pubDateStr=2026-02-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782450751970, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782450751970, creator=13701087609, updateTime=1782450751970, updator=13701087609, issue=Issue{id=1277254112370623080, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='2', pageStart='281', pageEnd='551', issueExtLink='null', onlineDate='null', pubDate='1771948800000', pubDateStr='2026-02-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782450710622, creator='13701087609', updateTime=1782451594955, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277257821632729830, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277257821632729831, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=519, endPage=530, ext={EN=ArticleExt(id=1277254286966919767, articleId=1277254285796708946, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Impact of Beauveria bassiana Infection on the Structure of Soil Microbial Community in Nests of Solenopsis invicta, columnId=1236292524264968282, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Protection & Bio-safety, runingTitle=null, highlight=null, articleAbstract=

The inoculation of biocontrol fungi may affect the community structure of microorganisms in the soil, and changes in soil microbial community structure may in turn affect the control effectiveness. This study focused on the most harmful invasive pest, the red imported fire ant Solenopsis invicta Buren. The highly pathogenic Beauveria bassiana strain HHY-B was applied to its nest to reveal the impact of pathogen infection on the nest microbial community structure of the red imported fire ant. The microbiome analysis was conducted on soil samples of B. bassiana infected ant nests after 48 and 96 hours, as well as uninfected ant nests using high-throughput sequencing technology. A total of 648 708 valid bacterial sequences were obtained, belonging to 38 phylum, 127 classes, 274 orders, 451 families and 897 genus. 898 443 fungal sequences were also obtained, belonging to 12 phylum, 38 classes, 88 orders, 197 families, 377 genus. The Proteobacteria and Actinobacteria dominated the soil bacterial community in ant nests. Treatment with B. bassiana significantly increased the relative abundance of Proteobacteria in ant nests (P<0.05), but had no significant effect on the relative abundance of Actinobacteria. Ascomycota dominated the fungal community in the nest soil, and its relative abundance treated with B. bassiana was significantly increased in ant nests (P<0.05). In terms of relative abundance at the genus level, the genus of Beauveria showed an dominate fungi in the two treatment groups, which was not found in the control group. The KEGG pathway analysis showed that the differential bacteria were mainly focused on metabolic pathways, biosynthesis of secondary metabolites, microbial metabolism in various environments, biosynthesis of amino acids, carbon metabolism, two-component systems, ribosomes and other metabolic pathways. The enrichment level of the pathways in the control group was higher than that in the treatment group. This study revealed the changes in the microbial community structure of the red imported fire ant nests after treatment with B. bassiana, which helps to the further understanding of the mechanism of B. bassiana on the red imported fire ants and the living environment, and provides theoretical basis for the biological control for the red imported fire ants.

, authors=null, authorsList=Qianqian MENG, Lianjie MA, Yonghao YU, Xiao GUO, Jiayi HUANGFU, Lulu GUO, Xiuzhen LONG, authorCompany=null, correspAuthors=Xiuzhen LONG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277254287881278042, articleId=1277254285796708946, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=球孢白僵菌侵染对红火蚁蚁巢内土壤微生物群落结构的影响, columnId=1236292524520820846, journalTitle=热带作物学报, columnName=植物保护与生物安全, runingTitle=null, highlight=null, articleAbstract=

生防真菌的介入会影响土壤中微生物的群落结构,而土壤微生物群落结构的改变反过来可能会影响其控制效果。本研究以最具危害性的重大入侵害虫红火蚁(Solenopsis invicta Buren)为对象,通过对蚁巢施用较强致病性的球孢白僵菌菌株HHY-B,揭示病原菌侵染前后对红火蚁蚁巢土壤微生物群落结构的影响。采用高通量测序技术对球孢白僵菌侵染蚁巢后48、96 h及未侵染蚁巢的土壤样品进行微生物组学分析,共获得细菌648 708条有效序列,隶属38门、127纲、274目、451科、897属;真菌898 443条序列,隶属12门、38纲、88目、197科、377属。变形菌门和放线菌门在蚁巢土壤细菌群落中占据主导地位,球孢白僵菌处理蚁巢显著提高了变形菌门的相对丰度(P<0.05),而对放线菌门的相对丰度无显著影响。子囊菌门在蚁巢土壤真菌群落中占据主导地位,球孢白僵菌处理蚁巢显著提高了子囊菌门的相对丰度(P<0.05)。从属水平相对丰度来看,处理组中增加了白僵菌属,且为优势菌群,对照组真菌群落中未发现有白僵菌属。差异细菌KEGG通路分析主要聚集在代谢通路、次级代谢产物的生物合成、多种环境中的微生物代谢、氨基酸的生物合成、碳代谢、双组分系统和核糖体等代谢途径中,对照组的富集程度高于处理组。本研究揭示了球孢白僵菌处理红火蚁蚁巢后巢内土壤微生物群落结构的变化,有助于深入了解球孢白僵菌对红火蚁及其生存环境的作用机制,为红火蚁的生物防治提供理论依据。

, authors=

孟倩倩(1985—),女,博士,副研究员,研究方向:害虫绿色防控。

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* 龙秀珍(LONG Xiuzhen),E-mail:
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孟倩倩(1985—),女,博士,副研究员,研究方向:害虫绿色防控。

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孟倩倩(1985—),女,博士,副研究员,研究方向:害虫绿色防控。

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球孢白僵菌侵染对红火蚁蚁巢内土壤微生物群落结构的影响
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孟倩倩 1 , 马连杰 1 , 于永浩 2 , 郭萧 1 , 皇甫佳一 1 , 郭璐璐 1 , 龙秀珍 2, *, *
热带作物学报 | 植物保护与生物安全 2026,47(2): 519-530
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热带作物学报 |植物保护与生物安全 2026 , 47 (2) : 519 -530
球孢白僵菌侵染对红火蚁蚁巢内土壤微生物群落结构的影响
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孟倩倩(1985—),女,博士,副研究员,研究方向:害虫绿色防控。

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孟倩倩(1985—),女,博士,副研究员,研究方向:害虫绿色防控。

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孟倩倩1, 马连杰1, 于永浩2, 郭萧1, 皇甫佳一1, 郭璐璐1, 龙秀珍2, *, *
作者信息
  • 1.重庆市农业科学院油料作物研究所,重庆 401329
  • 2.广西壮族自治区农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,广西南宁 530007
通讯作者:
* 龙秀珍(LONG Xiuzhen),E-mail:
Impact of Beauveria bassiana Infection on the Structure of Soil Microbial Community in Nests of Solenopsis invicta
Qianqian MENG1, Lianjie MA1, Yonghao YU2, Xiao GUO1, Jiayi HUANGFU1, Lulu GUO1, Xiuzhen LONG2
Affiliations
  • 1.Institute of Oil Crops, Chongqing Academy of Agricultural Sciences, Chongqing 401329, China
  • 2.Institute of Plant Protection, Guangxi Academy of Agricultural Sciences/Key Laboratory of Green Prevention and Control on Fruits and Vegetables in South China, Ministry of Agriculture and Rural Affairs/Guangxi Key Laboratory of Biology for Crop Diseases and Insect Pests, Nanning, Guangxi 530007, China
出版时间: 2026-02-25 doi: 10.3969/j.issn.1000-2561.2026.02.022
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生防真菌的介入会影响土壤中微生物的群落结构,而土壤微生物群落结构的改变反过来可能会影响其控制效果。本研究以最具危害性的重大入侵害虫红火蚁(Solenopsis invicta Buren)为对象,通过对蚁巢施用较强致病性的球孢白僵菌菌株HHY-B,揭示病原菌侵染前后对红火蚁蚁巢土壤微生物群落结构的影响。采用高通量测序技术对球孢白僵菌侵染蚁巢后48、96 h及未侵染蚁巢的土壤样品进行微生物组学分析,共获得细菌648 708条有效序列,隶属38门、127纲、274目、451科、897属;真菌898 443条序列,隶属12门、38纲、88目、197科、377属。变形菌门和放线菌门在蚁巢土壤细菌群落中占据主导地位,球孢白僵菌处理蚁巢显著提高了变形菌门的相对丰度(P<0.05),而对放线菌门的相对丰度无显著影响。子囊菌门在蚁巢土壤真菌群落中占据主导地位,球孢白僵菌处理蚁巢显著提高了子囊菌门的相对丰度(P<0.05)。从属水平相对丰度来看,处理组中增加了白僵菌属,且为优势菌群,对照组真菌群落中未发现有白僵菌属。差异细菌KEGG通路分析主要聚集在代谢通路、次级代谢产物的生物合成、多种环境中的微生物代谢、氨基酸的生物合成、碳代谢、双组分系统和核糖体等代谢途径中,对照组的富集程度高于处理组。本研究揭示了球孢白僵菌处理红火蚁蚁巢后巢内土壤微生物群落结构的变化,有助于深入了解球孢白僵菌对红火蚁及其生存环境的作用机制,为红火蚁的生物防治提供理论依据。

红火蚁  /  球孢白僵菌  /  蚁巢  /  土壤微生物群落  /  代谢调控

The inoculation of biocontrol fungi may affect the community structure of microorganisms in the soil, and changes in soil microbial community structure may in turn affect the control effectiveness. This study focused on the most harmful invasive pest, the red imported fire ant Solenopsis invicta Buren. The highly pathogenic Beauveria bassiana strain HHY-B was applied to its nest to reveal the impact of pathogen infection on the nest microbial community structure of the red imported fire ant. The microbiome analysis was conducted on soil samples of B. bassiana infected ant nests after 48 and 96 hours, as well as uninfected ant nests using high-throughput sequencing technology. A total of 648 708 valid bacterial sequences were obtained, belonging to 38 phylum, 127 classes, 274 orders, 451 families and 897 genus. 898 443 fungal sequences were also obtained, belonging to 12 phylum, 38 classes, 88 orders, 197 families, 377 genus. The Proteobacteria and Actinobacteria dominated the soil bacterial community in ant nests. Treatment with B. bassiana significantly increased the relative abundance of Proteobacteria in ant nests (P<0.05), but had no significant effect on the relative abundance of Actinobacteria. Ascomycota dominated the fungal community in the nest soil, and its relative abundance treated with B. bassiana was significantly increased in ant nests (P<0.05). In terms of relative abundance at the genus level, the genus of Beauveria showed an dominate fungi in the two treatment groups, which was not found in the control group. The KEGG pathway analysis showed that the differential bacteria were mainly focused on metabolic pathways, biosynthesis of secondary metabolites, microbial metabolism in various environments, biosynthesis of amino acids, carbon metabolism, two-component systems, ribosomes and other metabolic pathways. The enrichment level of the pathways in the control group was higher than that in the treatment group. This study revealed the changes in the microbial community structure of the red imported fire ant nests after treatment with B. bassiana, which helps to the further understanding of the mechanism of B. bassiana on the red imported fire ants and the living environment, and provides theoretical basis for the biological control for the red imported fire ants.

Solenopsis invicta  /  Beauveria bassiana  /  ant nest  /  soil microbial community  /  metabolic regulation
孟倩倩, 马连杰, 于永浩, 郭萧, 皇甫佳一, 郭璐璐, 龙秀珍. 球孢白僵菌侵染对红火蚁蚁巢内土壤微生物群落结构的影响. 热带作物学报, 2026 , 47 (2) : 519 -530 . DOI: 10.3969/j.issn.1000-2561.2026.02.022
Qianqian MENG, Lianjie MA, Yonghao YU, Xiao GUO, Jiayi HUANGFU, Lulu GUO, Xiuzhen LONG. Impact of Beauveria bassiana Infection on the Structure of Soil Microbial Community in Nests of Solenopsis invicta[J]. Chinese Journal of Tropical Crops, 2026 , 47 (2) : 519 -530 . DOI: 10.3969/j.issn.1000-2561.2026.02.022
  • 重庆市市级财政科技创新类项目青年专项基金项目(KYLX20240500083)
  • 广西重点研发计划项目(桂科AB23026073)
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2026年第47卷第2期
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doi: 10.3969/j.issn.1000-2561.2026.02.022
  • 接收时间:2025-10-26
  • 首发时间:2026-06-26
  • 出版时间:2026-02-25
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  • 收稿日期:2025-10-26
  • 录用日期:2025-11-20
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重庆市市级财政科技创新类项目青年专项基金项目(KYLX20240500083)
广西重点研发计划项目(桂科AB23026073)
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    1.重庆市农业科学院油料作物研究所,重庆 401329
    2.广西壮族自治区农业科学院植物保护研究所/农业农村部华南果蔬绿色防控重点实验室/广西作物病虫害生物学重点实验室,广西南宁 530007

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* 龙秀珍(LONG Xiuzhen),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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