Article(id=1277254247557230693, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.02.007, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1758384000000, receivedDateStr=2025-09-21, revisedDate=null, revisedDateStr=null, acceptedDate=1760976000000, acceptedDateStr=2025-10-21, onlineDate=1782450742854, onlineDateStr=2026-06-26, pubDate=1771948800000, pubDateStr=2026-02-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782450742854, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782450742854, creator=13701087609, updateTime=1782450742854, updator=13701087609, issue=Issue{id=1277254112370623080, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='2', pageStart='281', pageEnd='551', issueExtLink='null', onlineDate='null', pubDate='1771948800000', pubDateStr='2026-02-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782450710622, creator='13701087609', updateTime=1782451594955, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277257821632729830, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277257821632729831, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=350, endPage=363, ext={EN=ArticleExt(id=1277254249687937130, articleId=1277254247557230693, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Key Aroma Components and Biosynthesis Mechanisms Revealed by Integrated Metabolomics and Transcriptomics in Ocimum gratissimum, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Ocimum gratissimum is widely utilized due to its significant medicinal and culinary value. However, substantial variations in volatile components and medicinal metabolite content exist among different germplasms, influenced by geographical origin, cultivation conditions, and environmental factors. The variations directly impact the stability of pharmacological efficacy and industrial-scale production. To address this, the present study employed an integrated metabolomics and transcriptomics approach to systematically investigate the aroma profiles, metabolite composition, and regulatory mechanisms of two distinct O. gratissimum germplasms (G096 and G134). Sensory evaluation confirmed that both accessions exhibited a characteristic clove-like aroma. Metabolomic analysis identified 1094 volatile metabolites, with 784 showing significant differential accumulation between the two germplasms. Correlation analysis between differential metabolites and sensory attributes revealed that eugenol was the key metabolite responsible for the distinctive flavor of O. gratissimum. Transcriptomic profiling identified 54 936 differentially expressed genes (DEGs), including 27 220 upregulated and 27 716 downregulated genes. Through metabolic pathway prediction, a biosynthetic pathway for eugenol in O. gratissimum was constructed, identifying PAL, C4H, 4CL, HCT and C3'H as critical regulatory genes governing eugenol biosynthesis. This study would not only elucidate the biosynthetic mechanism of the primary flavor compounds in O. gratissimum, but also provide a crucial molecular theoretical foundation for quality improvement and standardized production. The findings would hold significant scientific and practical value for advancing the industrial development of O. gratissimum.

, authors=null, authorsList=Shidong LI, Shuren GAO, Fulai YU, Yiming FANG, Qian JIANG, Lingliang GUAN, Songbi CHEN, authorCompany=null, correspAuthors=Lingliang GUAN, Songbi CHEN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277254250484854892, articleId=1277254247557230693, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=丁香罗勒特征香味物质及代谢组、转录组联合分析其合成途径, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

丁香罗勒（Ocimum gratissimum）因其显著的药用和食用价值而被广泛应用。然而，不同地理来源的种质资源、栽培条件及环境因素导致其挥发性成分和药用代谢物含量存在显著差异，直接影响药效的稳定性和产业化发展。为此，本研究采用代谢组学和转录组学联合分析技术，系统研究2个不同来源丁香罗勒品种（G096和G134）的香味类型、代谢物组成及其调控机制。感官分析结果显示，2份材料均具有明显的丁香味；代谢组分析结果显示，2个丁香罗勒品种中共鉴定出1094种挥发性代谢物，其中差异代谢物784种；结合差异代谢物与感官分析表明，丁香酚是丁香罗勒独特香味的关键代谢物。转录组分析结果显示，2个丁香罗勒品种中共鉴定出54 936个差异表达基因，其中上调基因27 220个，下调基因27 716个；通过代谢通路预测，构建丁香罗勒中丁香酚生物合成途径，挖掘到PAL、C4H、4CL、HCT、C3’H是丁香酚生物合成的关键调控基因。本研究不仅阐明了丁香罗勒主要香味物质的生物合成机制，更为其品质改良和标准化生产提供重要的分子理论基础，对推动丁香罗勒产业化发展具有重要的科学意义和应用价值。

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李实东（2000—），男，硕士研究生，研究方向：罗勒优异种质筛选。

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李实东（2000—），男，硕士研究生，研究方向：罗勒优异种质筛选。

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