热带作物学报

咖啡S-腺苷甲硫氨酸合成酶基因CaSAMS3的克隆及表达模式分析

PDF下载

王曦奥 ¹^,²^,³^,⁴^,⁵ , 廖振阳 ¹^,²^,³^,⁴^,⁵^,⁶ , 安娜 ¹^,²^,³^,⁴^,⁵ , 胡丽松 ¹^,²^,³^,⁴^,⁵^,⁶ , 黄丽芳 ¹^,²^,³^,⁴^,⁵ , 赵新传 ⁷ , 熊文艳 ¹^,²^,³^,⁴^,⁵ , 庞永青 ¹^,²^,³^,⁴^,⁵ , 王韫镭 ¹^,⁴^,⁵ , 王晓阳 ¹^,²^,³^,⁴^,⁵ , William Solano SANCHEZ ³^,⁸ , 闫林 ¹^,²^,³^,⁴^,⁵^,^*^,^*

热带作物学报 | 组学与生物技术 2026,47(2): 302-312

收起

热带作物学报 |组学与生物技术 2026 , 47 (2) : 302 -312

咖啡S-腺苷甲硫氨酸合成酶基因CaSAMS3的克隆及表达模式分析

全屏

王曦奥¹^,²^,³^,⁴^,⁵, 廖振阳¹^,²^,³^,⁴^,⁵^,⁶, 安娜¹^,²^,³^,⁴^,⁵, 胡丽松¹^,²^,³^,⁴^,⁵^,⁶, 黄丽芳¹^,²^,³^,⁴^,⁵, 赵新传⁷, 熊文艳¹^,²^,³^,⁴^,⁵, 庞永青¹^,²^,³^,⁴^,⁵, 王韫镭¹^,⁴^,⁵, 王晓阳¹^,²^,³^,⁴^,⁵, William Solano SANCHEZ³^,⁸, 闫林¹^,²^,³^,⁴^,⁵^,^*^,^*

作者信息

^1．中国热带农业科学院香料饮料研究所，海南万宁　571533

^2．咖啡海南省工程研究中心，海南万宁　571533

^3．热带香料饮料国际联合研究中心，海南万宁　571533

^4．农业部香辛饮料作物遗传资源利用重点实验室，海南万宁　571533

^5．海南省热带香辛饮料作物遗传改良与品质调控重点实验室，海南万宁　571533

^6．热带作物生物育种全国重点实验室，海南三亚　572024

^7．云南农业大学热带作物学院，云南普洱　665000

^8．热带农业研究与高等教育中心，哥斯达黎加图里亚尔巴　30501

通讯作者:

^* 闫林（YAN Lin），E-mail: yanlin2575@163.com。

Molecular Cloning and Expression Pattern Analysis of S-adenosylmethionine Synthase Gene CaSAMS3 in Coffee

Xi'ao WANG¹^,²^,³^,⁴^,⁵, Zhenyang LIAO¹^,²^,³^,⁴^,⁵^,⁶, Na AN¹^,²^,³^,⁴^,⁵, Lisong HU¹^,²^,³^,⁴^,⁵^,⁶, Lifang HUANG¹^,²^,³^,⁴^,⁵, Xinchuan ZHAO⁷, Wenyan XIONG¹^,²^,³^,⁴^,⁵, Yongqing PANG¹^,²^,³^,⁴^,⁵, Yunlei WANG¹^,⁴^,⁵, Xiaoyang WANG¹^,²^,³^,⁴^,⁵, William Solano SANCHEZ³^,⁸, Lin YAN¹^,²^,³^,⁴^,⁵

Affiliations

^1.Spice and Beverage Research Institute, Chinese Academy of Tropical Agricultural Sciences, Wanning, Hainan 571533, China

^2.Coffee Hainan Engineering Research Center, Wanning, Hainan 571533, China

^3.International Joint Research Center for Tropical Spice and Beverage, Wanning, Hainan 571533, China

^4.Key Laboratory of Genetic Resources Utilization of Spice and Beverage Crops, Ministry of Agriculture & Rural Affairs, Wanning, Hainan 571533, China

^5.Hainan Provincial Key Laboratory of Genetic Improvement and Quality Regulation for Tropical Spice and Beverage Crops, Wanning, Hainan 571533, China

^6.National Key Laboratory of Tropical Crop Biological Breeding, Sanya, Hainan 572024, China

^7.College of Tropical Crops, Yunnan Agricultural University, Pu'er, Yunnan 665000, China

^8.Center for Tropical Agricultural Research and Higher Education, Turrialba 30501, Costa Rica

出版时间: 2026-02-25 doi: 10.3969/j.issn.1000-2561.2026.02.003

文章导航

摘要

收起

S-腺苷甲硫氨酸合成酶（S-adenosylmethionine synthase, SAMS）是催化S-腺苷甲硫氨酸（S-adenosylmethionine,SAM）合成的关键酶，广泛参与植物的表观遗传调控、氧化胁迫及生长发育等多项生物学过程。本研究克隆咖啡CaSAMS3基因的全长cDNA序列，对其编码蛋白进行理化性质、蛋白结构、保守结构域和进化特征分析，通过顺式作用元件分析和蛋白互作网络预测，探讨CaSAMS3参与的代谢过程，并结合qRT-PCR与亚细胞定位分析其表达模式。结果表明：CaSAMS3的开放阅读框长1173 bp，编码一个亲水性蛋白（分子量为42.57 kDa，等电点为6.07），其二级结构以α-螺旋和无规则卷曲为主，定位于细胞膜和细胞核；该蛋白具有典型SAMS保守结构域，与猕猴桃SAMS同源性最高；其启动子涉及光、激素、胁迫及生长发育相关响应元件，包含MYB和MYC结合位点；CaSAMS3存在组织特异性表达，且在发育阶段的表达趋势与咖啡因积累一致。本研究结果为解析CaSAMS3在咖啡因合成调控中的生物学功能提供重要理论依据，也为咖啡品质与抗性改良提供潜在靶点。

关键词

咖啡 / 咖啡因 / S-腺苷甲硫氨酸合成酶 / 基因克隆 / 表达模式

Abstract

收起

S-adenosylmethionine synthase (SAMS) is a key enzyme catalyzing the synthesis of S-adenosylmethionine (SAM, S-adenosylmethionine), widely involved in various biological processes in plants, such as epigenetic regulation, oxidative stress, and growth and development. In this study, the full-length cDNA sequence of CaSAMS3 was successfully cloned from coffee, and the physicochemical properties, protein structure, conserved domains, and evolutionary characteristics of the encoded CaSAMS3 protein were analyzed. Through cis-acting element analysis and protein interaction network prediction, and the metabolic processes involving CaSAMS3 were explored, and its expression pattern was analyzed in combination with qPCR and subcellular localization studies. The results showed that the open reading frame length of CaSAMS3 was 1173 bp, encoding a hydrophilic protein (molecular weight 42.57 kDa, theoretical isoelectric point 6.07), with a secondary structure mainly composed of α- helices and irregular curls, and located on cell membrane and nucleus. The protein contained a typical SAMS conserved domain and had the highest homology with SAMS in kiwifruit. Its promoter contained response elements related to light, hormones, stress, and growth and development, including MYB and MYC binding sites. CaSAMS3 exhibited tissue-specific expression, and its expression trend during developmental stages was consistent with accumulation pattern of caffeine. This study would provide an important theoretical basis for elucidating the biological function of CaSAMS3 in the regulation of caffeine synthesis, and provides a potential target for improving coffee quality and resistance.

Key words

coffee / caffeine / S-adenosylmethionine synthase / gene cloning / expression pattern

引用本文

王曦奥, 廖振阳, 安娜, 胡丽松, 黄丽芳, 赵新传, 熊文艳, 庞永青, 王韫镭, 王晓阳, William Solano SANCHEZ, 闫林. 咖啡S-腺苷甲硫氨酸合成酶基因CaSAMS3的克隆及表达模式分析. 热带作物学报, 2026 , 47 (2) : 302 -312 . DOI: 10.3969/j.issn.1000-2561.2026.02.003

Xi'ao WANG, Zhenyang LIAO, Na AN, Lisong HU, Lifang HUANG, Xinchuan ZHAO, Wenyan XIONG, Yongqing PANG, Yunlei WANG, Xiaoyang WANG, William Solano SANCHEZ, Lin YAN. Molecular Cloning and Expression Pattern Analysis of S-adenosylmethionine Synthase Gene CaSAMS3 in Coffee[J]. Chinese Journal of Tropical Crops, 2026 , 47 (2) : 302 -312 . DOI: 10.3969/j.issn.1000-2561.2026.02.003

基金

收起

国家现代农业产业技术体系项目(CARS-11)
海南省自然科学基金青年基金项目(324QN309)

参考文献

收起

文献

收起

参考文献引证文献

排序方式：

2026年第47卷第2期

PDF下载

引用本文

BibTeX

文章信息

doi: 10.3969/j.issn.1000-2561.2026.02.003

接收时间：2025-11-03
首发时间：2026-06-26
出版时间：2026-02-25

补充材料

相关文章

文章信息

作者

出版历史

收稿日期：2025-11-03
录用日期：2025-11-18

基金

国家现代农业产业技术体系项目(CARS-11)

海南省自然科学基金青年基金项目(324QN309)

作者信息

^1．中国热带农业科学院香料饮料研究所，海南万宁　571533

^2．咖啡海南省工程研究中心，海南万宁　571533

^3．热带香料饮料国际联合研究中心，海南万宁　571533

^4．农业部香辛饮料作物遗传资源利用重点实验室，海南万宁　571533

^5．海南省热带香辛饮料作物遗传改良与品质调控重点实验室，海南万宁　571533

^6．热带作物生物育种全国重点实验室，海南三亚　572024

^7．云南农业大学热带作物学院，云南普洱　665000

^8．热带农业研究与高等教育中心，哥斯达黎加图里亚尔巴　30501

通讯作者:

^* 闫林（YAN Lin），E-mail: yanlin2575@163.com。

参考文献

分享链接

https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2026.02.003

分享至

全文二维码

扫描看全文

引用本文

BibTeX

本文的引用情况

2种不同金属材料的力学参数

科 Family	属数 Number of genus	种数 Number of species	占总种数比例 Percentage of total species (%)	属 Genus	种数 Number of species	占总种数比例 Percentage of total species (%)
鹅膏菌科Amanitaceae	2	11	5.26	鹅膏菌属 Amanita	10	4.78
小菇科 Mycenaceae	2	12	5.74	丝盖伞属 Inocybe	5	2.39
多孔菌科 Polyporaceae	8	14	6.70	蜡蘑属 Laccaria	5	2.39
红菇科 Russulaceae	3	23	11.00	小皮伞属 Marasmius	6	2.87
				小菇属 Mycena	11	5.26
				光柄菇属 Pluteus	5	2.39
				红菇属 Russula	17	8.13
				栓菌属 Trametes	5	2.39

关闭全屏

BibTeX
EndNote
RefWorks
TxT