Article(id=1277254189273183124, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.01.017, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1759334400000, receivedDateStr=2025-10-02, revisedDate=null, revisedDateStr=null, acceptedDate=1761494400000, acceptedDateStr=2025-10-27, onlineDate=1782450728957, onlineDateStr=2026-06-26, pubDate=1769270400000, pubDateStr=2026-01-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782450728957, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782450728957, creator=13701087609, updateTime=1782450728957, updator=13701087609, issue=Issue{id=1277253949203809194, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='1', pageStart='1', pageEnd='280', issueExtLink='null', onlineDate='null', pubDate='1769270400000', pubDateStr='2026-01-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782450671721, creator='13701087609', updateTime=1782451647086, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277258041263256431, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277258041263256432, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=176, endPage=185, ext={EN=ArticleExt(id=1277254189784888215, articleId=1277254189273183124, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Screening and Verification of Reference Genes for qRT-PCR Analysis in Camellia hainanica under Low Phosphorus Stress, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Plants are prone to low inorganic phosphorus (Pi) abiotic stress, which has adverse effects on the growth, development and physiological metabolic processes. Camellia hainanica, a local variety in Hainan, mostly grows in low-phosphorus environments. C. hainanica has unique genetic characteristics that distinguish it from C. oleifera. In this study, a tropical cultivar Haida Oil-tea 4 (H4) was used as the research object to screen reference genes suitable for quantitative real-time polymerase chain reaction (qRT-PCR) analysis of C. hainanica under low Pi stress. The expression levels of six candidate reference genes, EF1α1, GAPDH, Actin, UBQ, ETIF3H and TUB2, were detected by qRT-PCR. The stability of each reference gene was comprehensively evaluated using ΔCt, geNorm, NormFinder, BestKeeper software and RefFinder online analysis tools, and ETIF3H was identified as the optimal reference gene. Using ETIF3H as the reference gene, we validated the expression levels of three low Pi responsive genes. The results showed that the target genes were differentially expressed in response to low Pi, indicating that ETIF3H is suitable as a reference gene for detecting gene differential expression under low Pi stress conditions. This work would provide appropriate reference genes for transcript normalization in H4 under low Pi stress, which will facilitate subsequent study of phosphorus signal-response gene expression in C. hainanica.

, authors=null, authorsList=Xiao WANG, Chengting YANG, Cen LIN, Jian WANG, Jing LI, authorCompany=null, correspAuthors=Jing LI, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277254191491969945, articleId=1277254189273183124, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=低磷胁迫下海南油茶实时荧光定量PCR内参基因筛选及验证, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

低磷（inorganic phosphate, Pi）胁迫是一种常见的非生物胁迫，对植物的生长发育及生理代谢过程产生不利影响。海南油茶多生长在低磷环境中，是特异地理小种，具有区别于内地普通油茶的独特遗传特性。本研究以海南热带油茶—海大油茶4号（H4）为材料，筛选适合在低磷胁迫下用于实时荧光定量PCR（qRT-PCR）分析的最佳内参基因。以低磷处理不同时间点的H4的根和叶组织为材料，使用qRT-PCR检测6个候选内参基因EF1α1、GAPDH、Actin、UBQ、ETIF3H、TUB2的表达水平，并采用ΔCt、geNorm、NormFinder、BestKeeper软件和RefFinder在线分析工具对各内参基因的稳定性进行综合评价，确定ETIF3H为最佳内参基因。以ETIF3H为内参基因，对3个低磷响应基因进行表达水平验证，结果显示目的基因均响应低磷且差异表达，表明ETIF3H适合作为低磷胁迫条件下基因差异表达检测的内参基因。本研究为低磷胁迫下H4油茶的转录本标准化提供了合适的参考基因，有利于后续对海南油茶中低磷响应基因的差异表达进行研究。

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王潇（2000—），女，硕士研究生，研究方向：分子植物育种。

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王潇（2000—），女，硕士研究生，研究方向：分子植物育种。

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