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Article(id=1277254171086680897, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.01.019, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1758384000000, receivedDateStr=2025-09-21, revisedDate=null, revisedDateStr=null, acceptedDate=1760803200000, acceptedDateStr=2025-10-19, onlineDate=1782450724622, onlineDateStr=2026-06-26, pubDate=1769270400000, pubDateStr=2026-01-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782450724622, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782450724622, creator=13701087609, updateTime=1782450724622, updator=13701087609, issue=Issue{id=1277253949203809194, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='1', pageStart='1', pageEnd='280', issueExtLink='null', onlineDate='null', pubDate='1769270400000', pubDateStr='2026-01-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782450671721, creator='13701087609', updateTime=1782451647086, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277258041263256431, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277258041263256432, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=197, endPage=208, ext={EN=ArticleExt(id=1277254171384476483, articleId=1277254171086680897, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Effects of Subculture Times on Somatic Embryogenesis and Key Organic Substance Metabolism in Litchi Embryogenic Callus, columnId=1236256434120348225, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Cultivation, Physiology & Biochemistry, runingTitle=null, highlight=null, articleAbstract=

Long-term subculture preservation significantly impacts somatic embryogenesis (SE) in various plant species. Studies on its effects in litchi (Litchi chinensis Sonn.) remain scarce. This study aimed to investigate the effects of subculture times on SE in litchi embryogenic callus (EC) and to uncover the metabolic basis for its decline, thereby providing a theoretical foundation for improving SE efficiency. Using 'Feizixiao' litchi EC materials from different subculture times (denoted as J11, J22, J48, J65 and J86). Somatic embryo induction rate, phenotypic characteristics, and dynamic changes in soluble sugars, organic acids, free amino acids, and energy-related substances were measured. The results showed that subcultures times significantly influenced SE in litchi EC. The embryo induction rate initially increased and then decreased with increasing subculture times, while the yield of normal somatic embryos (monocotyledonous, dicotyledonous, and polycotyledonous embryos) was also significantly affected. Among the five groups, J22 exhibited the highest induction rate (257.33%) and the greatest number of milky-white embryos. Sucrose content showed a significant positive correlation with SE, highlighting its role as the key carbon source for litchi SE. Fluctuations in soluble sugar content were primarily attributed to variations in sucrose. Low-subculture materials (J11 and J22) showed significant decreases in citric acid, succinic acid, and malic acid during the middle-late SE induction stages, indicating a stronger tricarboxylic acid (TCA) cycle flux compared to high-subculture materials (J48, J65, J86). The total amino acid content in EC initially increased and then stabilized during SE induction, with low-subculture materials maintaining higher overall amino acid levels. The contents of two major amino acids (glutamine and alanine) were significantly more abundant in low-subculture materials. Furthermore, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) initially increased and then decreased, with AMP being the most abundant. Low-subculture materials maintained significantly higher AMP levels during the first 49 days of SE induction, particularly in J22. The results demonstrate that subculture times is a critical factor influencing SE in litchi EC. Long-term subculture induces embryogenic decline by impacting energy supply and amino acid metabolism.

, authors=null, authorsList=Qiurong SUN, Guo WANG, Zhaoyin GAO, Shujun WANG, Huanling LI, Hongna ZHANG, Xiaoxu LI, Jiabao WANG, authorCompany=null, correspAuthors=Xiaoxu LI, Jiabao WANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277254171724215109, articleId=1277254171086680897, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=继代次数对荔枝胚性愈伤组织体胚诱导及重要有机物代谢的影响, columnId=1236256434313286224, journalTitle=热带作物学报, columnName=作物栽培与生理生化, runingTitle=null, highlight=null, articleAbstract=

长期继代保存会对多种植物的体胚发生效率产生影响，但目前在荔枝（Litchi Chinensis Sonn.）上尚未见相关研究报道。本研究旨在明确继代次数对荔枝胚性愈伤组织（embryogenic callus, EC）体细胞胚胎发生（somatic embryogenesis, SE）的影响，揭示其胚性衰退的代谢基础，为提高愈伤组织体胚发生效率提供理论依据。本研究以妃子笑荔枝不同继代次数（继代11、22、48、65、86代）愈伤组织为材料，测定其体胚诱导率及表型状态，分析可溶性糖、有机酸、游离氨基酸及能量物质含量的动态变化。结果显示：继代次数对荔枝愈伤组织的体胚发生具有显著影响，体胚诱导率随继代次数的增加呈先升高后降低的趋势，同时对正常体胚（单子叶胚、双子叶胚和多子叶胚）的诱导数量也有显著影响。在5组材料中，J22的体胚诱导率最高，为257.33%，乳白胚数量最多。蔗糖含量与体胚诱导率呈显著正相关，是荔枝体胚发生的关键碳源，可溶性糖含量的变化主要源于蔗糖含量的变化。低继代次数材料（J11和J22）在体胚诱导中后期，柠檬酸、琥珀酸和苹果酸含量显著下降，表明其TCA循环通量要强于高继代次数材料（J48、J65和J86）。在体胚诱导过程中，愈伤组织中的总氨基酸含量先上升后趋于平稳，总体上，低继代次数材料EC总氨基酸含量要高于高继代次数材料。低继代次数材料中2种主要氨基酸（谷氨酰胺和丙氨酸）含量显著高于高继代次数材料。此外，腺苷三磷酸（ATP）、腺苷二磷酸（ADP）、腺苷一磷酸（AMP）含量均表现出先增加后降低的趋势，其中AMP含量最高。低继代次数材料愈伤组织中AMP含量在体胚诱导0~49 d时均显著高于高继代次数材料，其中J22愈伤组织AMP的含量最高。这些结果表明，继代次数是荔枝愈伤组织体胚发生的关键影响因子。长期继代通过影响能量供应与氨基酸代谢，导致荔枝愈伤组织胚性衰退。

, authors=

孙秋容（2001—），女，硕士研究生，研究方向：荔枝离体再生机制。

, authorsList=孙秋容, 王果, 高兆银, 王树军, 李焕苓, 张红娜, 李晓旭, 王家保, authorCompany=null, correspAuthors=李晓旭, 王家保, authorNote=null, correspAuthorsNote=
* 王家保（WANG Jiabao），E-mail:
李晓旭（LI Xiaoxu），E-mail: 。
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2.Institute of Environment and Plant Protection Research, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
3.National Key Labora-tory for Tropical Crop Breeding, Haikou, Hainan 571101, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1277254174618284890, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277254171086680897, authorId=1277254174123357013, language=CN, stringName=孙秋容, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=1, 2, 3, address=1.海南大学热带农林学院，海南儋州　571737
2.中国热带农业科学院环境与植物保护研究所，海南海口　571101
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孙秋容（2001—），女，硕士研究生，研究方向：荔枝离体再生机制。

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孙秋容（2001—），女，硕士研究生，研究方向：荔枝离体再生机制。

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继代次数对荔枝胚性愈伤组织体胚诱导及重要有机物代谢的影响
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孙秋容 1, 2, 3 , 王果 2, 3 , 高兆银 4 , 王树军 2, 3 , 李焕苓 2, 3 , 张红娜 1 , 李晓旭 2, 3, * , 王家保 3, 4, *
热带作物学报 | 作物栽培与生理生化 2026,47(1): 197-208
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热带作物学报 |作物栽培与生理生化 2026 , 47 (1) : 197 -208
继代次数对荔枝胚性愈伤组织体胚诱导及重要有机物代谢的影响
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孙秋容（2001—），女，硕士研究生，研究方向：荔枝离体再生机制。

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孙秋容（2001—），女，硕士研究生，研究方向：荔枝离体再生机制。

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孙秋容1, 2, 3, 王果2, 3, 高兆银4, 王树军2, 3, 李焕苓2, 3, 张红娜1, 李晓旭2, 3, * , 王家保3, 4, *
作者信息
  • 1.海南大学热带农林学院，海南儋州　571737
  • 2.中国热带农业科学院环境与植物保护研究所，海南海口　571101
  • 3.热带作物生物育种全国重点实验室，海南海口　571101
  • 4.中国热带农业科学院热带作物品种资源研究所，海南海口　571101
通讯作者:
* 王家保（WANG Jiabao），E-mail:
李晓旭（LI Xiaoxu），E-mail: 。
Effects of Subculture Times on Somatic Embryogenesis and Key Organic Substance Metabolism in Litchi Embryogenic Callus
Qiurong SUN1, 2, 3, Guo WANG2, 3, Zhaoyin GAO4, Shujun WANG2, 3, Huanling LI2, 3, Hongna ZHANG1, Xiaoxu LI2, 3, * , Jiabao WANG3, 4, *
Affiliations
  • 1.School of Tropical Agriculture and Forestry, Hainan University, Danzhou, Hainan 571737, China
  • 2.Institute of Environment and Plant Protection Research, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
  • 3.National Key Labora-tory for Tropical Crop Breeding, Haikou, Hainan 571101, China
  • 4.Institute of Tropical Crops Germplasm Resources Research, Chinese Academy of Tropical Agricultural Sciences, Haikou, Hainan 571101, China
出版时间: 2026-01-25 doi: 10.3969/j.issn.1000-2561.2026.01.019
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长期继代保存会对多种植物的体胚发生效率产生影响，但目前在荔枝（Litchi Chinensis Sonn.）上尚未见相关研究报道。本研究旨在明确继代次数对荔枝胚性愈伤组织（embryogenic callus, EC）体细胞胚胎发生（somatic embryogenesis, SE）的影响，揭示其胚性衰退的代谢基础，为提高愈伤组织体胚发生效率提供理论依据。本研究以妃子笑荔枝不同继代次数（继代11、22、48、65、86代）愈伤组织为材料，测定其体胚诱导率及表型状态，分析可溶性糖、有机酸、游离氨基酸及能量物质含量的动态变化。结果显示：继代次数对荔枝愈伤组织的体胚发生具有显著影响，体胚诱导率随继代次数的增加呈先升高后降低的趋势，同时对正常体胚（单子叶胚、双子叶胚和多子叶胚）的诱导数量也有显著影响。在5组材料中，J22的体胚诱导率最高，为257.33%，乳白胚数量最多。蔗糖含量与体胚诱导率呈显著正相关，是荔枝体胚发生的关键碳源，可溶性糖含量的变化主要源于蔗糖含量的变化。低继代次数材料（J11和J22）在体胚诱导中后期，柠檬酸、琥珀酸和苹果酸含量显著下降，表明其TCA循环通量要强于高继代次数材料（J48、J65和J86）。在体胚诱导过程中，愈伤组织中的总氨基酸含量先上升后趋于平稳，总体上，低继代次数材料EC总氨基酸含量要高于高继代次数材料。低继代次数材料中2种主要氨基酸（谷氨酰胺和丙氨酸）含量显著高于高继代次数材料。此外，腺苷三磷酸（ATP）、腺苷二磷酸（ADP）、腺苷一磷酸（AMP）含量均表现出先增加后降低的趋势，其中AMP含量最高。低继代次数材料愈伤组织中AMP含量在体胚诱导0~49 d时均显著高于高继代次数材料，其中J22愈伤组织AMP的含量最高。这些结果表明，继代次数是荔枝愈伤组织体胚发生的关键影响因子。长期继代通过影响能量供应与氨基酸代谢，导致荔枝愈伤组织胚性衰退。

荔枝  /  愈伤组织  /  继代次数  /  体胚发生  /  代谢调控

Long-term subculture preservation significantly impacts somatic embryogenesis (SE) in various plant species. Studies on its effects in litchi (Litchi chinensis Sonn.) remain scarce. This study aimed to investigate the effects of subculture times on SE in litchi embryogenic callus (EC) and to uncover the metabolic basis for its decline, thereby providing a theoretical foundation for improving SE efficiency. Using 'Feizixiao' litchi EC materials from different subculture times (denoted as J11, J22, J48, J65 and J86). Somatic embryo induction rate, phenotypic characteristics, and dynamic changes in soluble sugars, organic acids, free amino acids, and energy-related substances were measured. The results showed that subcultures times significantly influenced SE in litchi EC. The embryo induction rate initially increased and then decreased with increasing subculture times, while the yield of normal somatic embryos (monocotyledonous, dicotyledonous, and polycotyledonous embryos) was also significantly affected. Among the five groups, J22 exhibited the highest induction rate (257.33%) and the greatest number of milky-white embryos. Sucrose content showed a significant positive correlation with SE, highlighting its role as the key carbon source for litchi SE. Fluctuations in soluble sugar content were primarily attributed to variations in sucrose. Low-subculture materials (J11 and J22) showed significant decreases in citric acid, succinic acid, and malic acid during the middle-late SE induction stages, indicating a stronger tricarboxylic acid (TCA) cycle flux compared to high-subculture materials (J48, J65, J86). The total amino acid content in EC initially increased and then stabilized during SE induction, with low-subculture materials maintaining higher overall amino acid levels. The contents of two major amino acids (glutamine and alanine) were significantly more abundant in low-subculture materials. Furthermore, the contents of adenosine triphosphate (ATP), adenosine diphosphate (ADP), and adenosine monophosphate (AMP) initially increased and then decreased, with AMP being the most abundant. Low-subculture materials maintained significantly higher AMP levels during the first 49 days of SE induction, particularly in J22. The results demonstrate that subculture times is a critical factor influencing SE in litchi EC. Long-term subculture induces embryogenic decline by impacting energy supply and amino acid metabolism.

Litchi chinensis Sonn.  /  callus tissue  /  subcultures number  /  somatic embryogenesis (SE)  /  metabolic regulation
孙秋容, 王果, 高兆银, 王树军, 李焕苓, 张红娜, 李晓旭, 王家保. 继代次数对荔枝胚性愈伤组织体胚诱导及重要有机物代谢的影响. 热带作物学报, 2026 , 47 (1) : 197 -208 . DOI: 10.3969/j.issn.1000-2561.2026.01.019
Qiurong SUN, Guo WANG, Zhaoyin GAO, Shujun WANG, Huanling LI, Hongna ZHANG, Xiaoxu LI, Jiabao WANG. Effects of Subculture Times on Somatic Embryogenesis and Key Organic Substance Metabolism in Litchi Embryogenic Callus[J]. Chinese Journal of Tropical Crops, 2026 , 47 (1) : 197 -208 . DOI: 10.3969/j.issn.1000-2561.2026.01.019
基金
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  • 海南省科技专项(ZDYF2022XDNY253)
  • 国家自然科学基金项目(32402522)
  • 国家荔枝龙眼产业技术体系项目(CARS-32)
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doi: 10.3969/j.issn.1000-2561.2026.01.019
  • 接收时间：2025-09-21
  • 首发时间：2026-06-26
  • 出版时间：2026-01-25
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  • 收稿日期：2025-09-21
  • 录用日期：2025-10-19
基金
海南省科技专项(ZDYF2022XDNY253)
国家自然科学基金项目(32402522)
国家荔枝龙眼产业技术体系项目(CARS-32)
作者信息
    1.海南大学热带农林学院，海南儋州　571737
    2.中国热带农业科学院环境与植物保护研究所，海南海口　571101
    3.热带作物生物育种全国重点实验室，海南海口　571101
    4.中国热带农业科学院热带作物品种资源研究所，海南海口　571101

通讯作者:

* 王家保（WANG Jiabao），E-mail:
李晓旭（LI Xiaoxu），E-mail: 。
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https://castjournals.cast.org.cn/joweb/rdzwxb/CN/10.3969/j.issn.1000-2561.2026.01.019
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2种不同金属材料的力学参数
科
Family		 属数
Number of
genus		 种数
Number of
species		 占总种数比例
Percentage of
total species (%)		 属
Genus		 种数
Number of
species		 占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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