Article(id=1277254153067954902, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.02.015, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1763308800000, receivedDateStr=2025-11-17, revisedDate=null, revisedDateStr=null, acceptedDate=1764950400000, acceptedDateStr=2025-12-06, onlineDate=1782450720325, onlineDateStr=2026-06-26, pubDate=1771948800000, pubDateStr=2026-02-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782450720325, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782450720325, creator=13701087609, updateTime=1782450720325, updator=13701087609, issue=Issue{id=1277254112370623080, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='2', pageStart='281', pageEnd='551', issueExtLink='null', onlineDate='null', pubDate='1771948800000', pubDateStr='2026-02-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782450710622, creator='13701087609', updateTime=1782451594955, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277257821632729830, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277257821632729831, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277254112370623080, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=444, endPage=458, ext={EN=ArticleExt(id=1277254154338829018, articleId=1277254153067954902, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Construction and Comprehensive Evaluation of an Evaluation System for Low-temperature and Weak-light Tolerance in Melon at the Seedling Stage, columnId=1236256434120348225, journalTitle=Chinese Journal of Tropical Crops, columnName=Plant Cultivation, Physiology & Biochemistry, runingTitle=null, highlight=null, articleAbstract=

Ten melon germplasm accessions were treated under simulated low-temperature and weak-light conditions in this study to establish an efficient identification system for low-temperature and weak-light tolerance of melon (Cucumis melo L.) germplasm at the seedling stage. The relative changes in 8 growth indicators (including plant height, stem diameter, leaf area, etc.) and 10 physiological indicators (including chlorophyll content, root activity, relative electrical conductivity, etc.) were determined, and the damage indexs were calculated to initially evaluate the low-temperature and weak-light tolerance of each germplasm. Based on the membership function method in fuzzy mathematics, 19 identification indicators were standardized. Dimensionality reduction was performed via principal component analysis (PCA), and 5 principal components with strong correlations to the low-temperature and weak-light tolerance index, peroxidase (POD) activity, root length, root activity, and soluble protein content were extracted (cumulative contribution rate: 87.026%). Cluster analysis was conducted on the 10 melon germplasm accessions, which were classified into three groups. Group Ⅰ including Vedrantais and M18, was classified as strong low-temperature and weak-light tolerant. Group Ⅱ includding IranH, Topmark, M4 and Nantais oblong, was classified as moderate low-temperature and weak-light tolerant. Group Ⅲ including PI124111, MR1, M76 and PMR5, was classified as weak low-temperature and weak-light tolerant. Finally, stepwise linear regression was used to establish a prediction model for low-temperature and weak-light tolerance of melon at the seedling stage, D=-0.058+0.008X₁₅+0.442X₉+0.058X₁-0.017X₁₉ (R²=0.998, P<0.001), X₁₅ is proline content; X₉ is root length; X₁ is low temperature and low light tolerance index; X₁₉ is peroxidase activity. To verify the reliability of the above identification results, the expression patterns of CmADF1 and CmCYP195 (key genes responding to low-temperature and weak-light stress) were analyzed in germplasm accessions M4, PI124111 and PMR5 at different time points after low-temperature and weak-light treatment. The results showed that the expression characteristics of CmADF1 and CmCYP195 were consistent with the identified low-temperature and weak-light tolerance types of the germplasm, which further confirmed the reliability of the identification system. The results could serve as identification criteria for evaluating low-temperature and weak-light tolerance of different melon germplasm accessions and provide breeding materials for subsequent improvement of melon varieties with low-temperature and weak-light tolerance.

, authors=null, authorsList=Shihan ZENG, Sang SHANG, Libo TIAN, Feifan CHEN, Xuzhen LI, authorCompany=null, correspAuthors=Sang SHANG, Libo TIAN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277254156926714590, articleId=1277254153067954902, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=甜瓜苗期耐低温弱光评价体系构建及综合评价, columnId=1236256434313286224, journalTitle=热带作物学报, columnName=作物栽培与生理生化, runingTitle=null, highlight=null, articleAbstract=

为建立甜瓜种质资源苗期耐低温弱光性的高效鉴定体系，本研究模拟低温弱光环境处理10份甜瓜种质，测定株高、茎粗、叶面积等8个生长指标和叶绿素含量、根系活力、相对电导率等10个生理指标的相对变化值，并计算伤害指数，初步评价各种质的耐低温弱光性。基于模糊数学中的隶属函数法对19个鉴定指标进行标准化处理，并通过主成分分析降维，提取出与耐低温弱光指数、过氧化物酶活性、根长、根系活力和可溶性蛋白含量关系较大的5个主成分（累积贡献率为87.026%）。对10份甜瓜种质进行聚类分析，第Ⅰ类包括Vedrantais和M18，属于强耐低温弱光型；第Ⅱ类包括IranH、Topmark、M4和Nantais oblong，属于中等耐低温弱光型；第Ⅲ类包括PI124111、MR1、M76和PMR5，属于弱耐低温弱光型。最后进行逐步线性回归，建立甜瓜苗期耐低温弱光性的预测模型：D=-0.058+0.008X₁₅+0.442X₉+0.058X₁-0.017X₁₉（R²=0.998，P<0.001），X₁₅为脯氨酸含量；X₉为根长；X₁为耐低温弱光指数；X₁₉为过氧化物酶活性。为验证上述鉴定结果的可靠性，分析了M4、PI124111和PMR5在低温弱光处理后不同时间点的响应低温弱光胁迫的关键基因CmADF1和CmCYP195的表达模式，结果表明，CmADF1和CmCYP195的表达特征与鉴定出的种质耐低温弱光类型一致，这进一步证实了该鉴定体系的可靠性。这些结果可作为鉴定不同甜瓜种质耐低温弱光性的鉴定标准，为后续甜瓜耐低温弱光品种改良提供育种材料。

, authors=, authorsList=曾诗涵, 商桑, 田丽波, 陈菲帆, 李许真, authorCompany=null, correspAuthors=商桑, 田丽波, authorNote=null, correspAuthorsNote=, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=OAwB43NGhJA+MS4Exipmyw==, magXml=DeLlMk54ZyK6ER1C6drj+w==, pdfUrl=null, pdf=ATIUy9FJqg1y1nGEJwVV5A==, pdfFileSize=2408455, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=jOkPij56vQodjE+At5TJQw==, mapNumber=null, fund=null)}, authors=[Author(id=1277254158155645672, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277254153067954902, orderNo=0, firstName=null, middleName=null, lastName=null, nameCn=null, orcid=null, stid=null, country=null, authorPic=null, dead=0, email=null, emailSecond=null, emailThird=null, correspondingAuthor=0, authorType=1, ext={EN=AuthorExt(id=1277254158503772907, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277254153067954902, authorId=1277254158155645672, language=EN, stringName=Shihan ZENG, firstName=Shihan, middleName=null, lastName=ZENG, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1.School of Life and Health Sciences, Hainan University, Haikou, Hainan 570228, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1277254160189883116, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277254153067954902, authorId=1277254158155645672, language=CN, stringName=曾诗涵, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=¹, address=^1．海南大学生命健康学院，海南海口　570228, bio={"content":"

曾诗涵（2002—），女，硕士研究生，研究方向：甜瓜耐低温弱光相关基因挖掘。

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曾诗涵（2002—），女，硕士研究生，研究方向：甜瓜耐低温弱光相关基因挖掘。

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