Article(id=1277254046452941121, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2026.01.016, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1756396800000, receivedDateStr=2025-08-29, revisedDate=null, revisedDateStr=null, acceptedDate=1760716800000, acceptedDateStr=2025-10-18, onlineDate=1782450694907, onlineDateStr=2026-06-26, pubDate=1769270400000, pubDateStr=2026-01-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782450694907, onlineIssueDateStr=2026-06-26, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782450694907, creator=13701087609, updateTime=1782450694907, updator=13701087609, issue=Issue{id=1277253949203809194, tenantId=1146029695717560320, journalId=1235980609244409860, year='2026', volume='47', issue='1', pageStart='1', pageEnd='280', issueExtLink='null', onlineDate='null', pubDate='1769270400000', pubDateStr='2026-01-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782450671721, creator='13701087609', updateTime=1782451647086, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1277258041263256431, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1277258041263256432, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1277253949203809194, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=165, endPage=175, ext={EN=ArticleExt(id=1277254046738153795, articleId=1277254046452941121, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Bioinformatics and Expression Analysis of the AcoCPKs Gene Family in Pineapple, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Calcium-dependent protein kinases (CPKs) are a class of plant-specific key signal transduction factors. In pineapple, an important tropical economic crop, the identification, classification, and functional characterization of the CPKs gene family are still in their infancy, and the molecular mechanisms by which they participate in stress responses remain unclear, which limits their application in the improvement of stress-resistant pineapple varieties. In this study, we systematically investigated the AcoCPKs gene family in pineapple by integrating genomic analysis, chromosomal localization, bioinformatic prediction, and qRT-PCR–based molecular approaches. A total of 18 AcoCPKs family members were identified at the whole-genome level in pineapple. Chromosomal mapping showed that these AcoCPKs genes are unevenly distributed across the nine chromosomes of pineapple. Bioinformatic analysis revealed that all AcoCPKs sproteins share similar conserved motifs and domain architectures. qRT-PCR expression profiling further demonstrated that the 18 AcoCPKs genes are expressed in multiple tissues, including fruits, leaves, fruit peduncles, and flower buds, with distinct tissue-specific expression patterns; moreover, the transcription levels of several AcoCPKs genes were markedly up-regulated under high-temperature, low-temperature, and NaCl salt stress treatments.Taken together, this study systematically elucidates the physicochemical properties, genetic characteristics, and stress-responsive expression patterns of members of the AcoCPKs gene family in pineapple. These findings not only provide an important theoretical basis for further dissecting the molecular mechanisms underlying AcoCPKs-mediated stress responses in pineapple, but also lay a solid foundation for the targeted improvement of stress tolerance and the breeding of high-quality, stress-resistant pineapple cultivars through gene editing, transgenic approaches, and other modern molecular breeding technologies.

, authors=null, authorsList=Linpan CHEN, Miaolin ZHANG, Yanli YAO, Xiaowan HOU, Qingsong WU, Junjun HE, Wuqiang MA, Chuanling LI, Xiumei ZHANG, authorCompany=null, correspAuthors=Chuanling LI, Xiumei ZHANG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1277254048797557061, articleId=1277254046452941121, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=菠萝AcoCPKs家族基因鉴定及表达分析, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

钙依赖性蛋白激酶（calcium-dependent protein kinases, CPKs）是植物特有的重要信号转导因子，在重要热带经济作物菠萝中，CPKs基因家族的鉴定、分类及功能研究仍处于起步阶段，其参与逆境响应的分子机制尚不清晰，难以满足菠萝抗逆品种改良的实践需求。本研究综合运用基因组学分析、染色体定位、生物信息学预测及qRT-PCR等分子生物学技术，对菠萝AcoCPKs基因家族进行系统研究。结果显示：从基因组水平共鉴定出18个AcoCPKs基因家族成员，这些AcoCPKs不均匀地分布于菠萝的9条染色体上。生物信息学分析显示，所有AcoCPKs蛋白均具有相似的保守基序与结构域特征。定量实时荧光PCR（qRT-PCR）表达分析进一步表明，18个AcoCPKs基因在果实、叶片、果柄和花蕾等不同组织中均有表达，且呈现明显的组织特异性；其中部分AcoCPKs基因在高温、低温及NaCl盐胁迫处理条件下转录水平显著上调。 综上，本研究系统解析了菠萝AcoCPKs基因家族成员的理化性质、遗传特征及其在逆境胁迫下的表达响应规律，不仅为深入揭示AcoCPKs介导的菠萝逆境响应分子机制提供重要理论依据，也为利用基因编辑、转基因等现代分子育种技术定向提升菠萝抗逆性、培育耐逆性强的优质新品种奠定坚实基础。

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^2.Institute of South Subtropical Crops, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs / Key Laboratory of Postharvest Physiology and Preservation of Tropical Horticultural Products, Hainan Province, Zhanjiang, Guangdong 524091, China, bio=null, bioImg=null, bioContent=null, aboutCorrespAuthor=null), CN=AuthorExt(id=1277254049896464728, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1277254046452941121, authorId=1277254049447674195, language=CN, stringName=陈林攀, firstName=null, middleName=null, lastName=null, prefix=null, suffix=null, authorComment=null, nameInitials=null, affiliation=null, department=null, xref=^{1, 2}, address=^1.海南大学热带农林学院，海南海口　570228
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陈林攀（2000—），男，硕士研究生，研究方向：菠萝抗病关键基因挖掘。

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陈林攀（2000—），男，硕士研究生，研究方向：菠萝抗病关键基因挖掘。

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