Article(id=1276601400691921445, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1276601397818814642, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2025.01.010, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1716307200000, receivedDateStr=2024-05-22, revisedDate=1725897600000, revisedDateStr=2024-09-10, acceptedDate=null, acceptedDateStr=null, onlineDate=1782295092031, onlineDateStr=2026-06-24, pubDate=1737734400000, pubDateStr=2025-01-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782295092031, onlineIssueDateStr=2026-06-24, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782295092031, creator=13701087609, updateTime=1782295092031, updator=13701087609, issue=Issue{id=1276601397818814642, tenantId=1146029695717560320, journalId=1235980609244409860, year='2025', volume='46', issue='1', pageStart='1', pageEnd='245', issueExtLink='null', onlineDate='null', pubDate='1737734400000', pubDateStr='2025-01-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782295091347, creator='13701087609', updateTime=1782295207335, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1276601884408418422, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1276601397818814642, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1276601884408418423, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1276601397818814642, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=94, endPage=105, ext={EN=ArticleExt(id=1276601401409147431, articleId=1276601400691921445, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Collection, Identification and Evaluation of Narenga porphyrocoma Germplasm Resources in Guangxi, columnId=1236256430060261740, journalTitle=Chinese Journal of Tropical Crops, columnName=Germplasm Resources, Genetics & Breeding, runingTitle=null, highlight=null, articleAbstract=

Guangxi spans the southern subtropics and central subtropics, with the Tropic of Cancer crossing the central part of the province, and belongs to the subtropical monsoon climate zone, which breeds rich wild sugarcane germplasm resources. From 2016 to 2023, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences organized a team of researchers to carry out systematic surveys and collection of Narenga porphyrocoma germplasm resources in Guangxi. The team collected a total of 58 copies of N. porphyrocoma germplasm resources in 43 townships of 18 cities and districts in Guangxi. The results showed that the N. porphyrocoma germplasm resources in Guangxi were mainly distributed in the area of 60.0-602.5 m above sea level, longitude E: 107°08′01″-111°32′02″, latitude N: 23°08′02″-26°01′43″. The genetic diversity indices of the 20 descriptive quality traits of N. porphyrocoma germplasm resources in Guangxi ranged from 0 to 1.2044, with a mean value of 0.5557, in which the diversity of the color of the internodes after exposure was the highest, that of the cork was lower, and the diversity of the three traits of stem shape, aerial roots and water splitting was not reflected; the genetic diversity indices of N. porphyrocoma germplasm resources in Guangxi of the different collection areas ranged from 0.1040 to 0.4911, with Hezhou being the highest and Baise being the lowest. The phenotypic traits of N. porphyrocoma germplasm resources in Guangxi were rich in variation, and the coefficients of variation for six quantitative traits ranged from 17.3% to 53.6%, with an average of 34.8%; the coefficients of variation of N. porphyrocoma germplasm resources in Guangxi from different collection areas ranged from 29.4% to 36.9%, with Hechi being the largest and Guilin being the smallest. The results of cluster analysis showed that the 58 N. porphyrocoma germplasm resources in Guangxi were classified into six major groups, but were not closely related to the geographical origin, in which the materials in the first major group had better overall traits and excellent biomass and quality traits. The collection expanded the number of resources in Guangxi Sugarcane Germplasm Repository and would provide excellent genetic resources for sugarcane breeding and genetic improvement.

, authors=null, authorsList=Wenyi ZONG, Baoqing ZHANG, Gemin ZHANG, Yuxin HUANG, Cuifang YANG, Shan ZHOU, Yijing GAO, Faqian XIONG, Jing LIU, Shanyu LU, Xiping YANG, Weixing DUAN, authorCompany=null, correspAuthors=Xiping YANG, Weixing DUAN, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1276601402365448749, articleId=1276601400691921445, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=广西河八王种质资源考察收集与鉴定评价, columnId=1236256430219645304, journalTitle=热带作物学报, columnName=种质资源与遗传育种, runingTitle=null, highlight=null, articleAbstract=

广西地跨南亚热带和中亚热带,北回归线横贯中部,属亚热带季风气候区,孕育了丰富的野生甘蔗种质资源。2016—2023年,广西农业科学院甘蔗研究所组织考察队对广西河八王种质资源开展系统调查和收集工作,考察队在广西8个地级市18个县(市、区)43个乡镇共收集到河八王种质资源58份。调查结果表明:广西河八王种质资源主要分布在海拔60.0~602.5 m,北纬23°08′02″~26°01′43″,东经107°08′01″~111°32′02″的区域;广西河八王种质资源20个描述型质量性状的遗传多样性指数在0~1.2044之间,平均值为0.5557,其中曝光后节间颜色的多样性最高,木栓较低,而茎形、气根、水裂3个性状无多样性体现;不同收集地区的广西河八王种质资源遗传多样性指数在0.1040~0.4911之间,贺州最高,百色最低;广西河八王种质资源表型性状变异丰富,6个数量性状的变异系数为17.3%~53.6%,平均为34.8%;不同收集地区的广西河八王种质资源变异系数在29.4%~36.9%之间,河池最大,桂林最小。聚类分析结果显示,58份广西河八王种质资源被分为六大类,与地理来源无密切关系,其中第Ⅰ类群中的材料综合性状较好,生物量和品质性状优良。本次考察收集扩充了广西甘蔗种质资源圃的资源数量,为甘蔗育种和遗传改良提供了优良基因资源。

, authors=

宗文一(2001—),男,硕士研究生,研究方向:甘蔗遗传育种。

, authorsList=宗文一, 张保青, 张革民, 黄玉新, 杨翠芳, 周珊, 高轶静, 熊发前, 刘菁, 陆衫羽, 杨细平, 段维兴, authorCompany=null, correspAuthors=杨细平, 段维兴, authorNote=null, correspAuthorsNote=
* 杨细平(YANG Xiping),E-mail:
段维兴(DUAN Weixing),E-mail:
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宗文一(2001—),男,硕士研究生,研究方向:甘蔗遗传育种。

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(in Chinese), articleTitle=Phenotypic traits and genetic diversity of Erianthus arundinaceum germplasm from Guangxi, refAbstract=null), Reference(id=1276601978411151410, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2023, volume=44, issue=6, pageStart=1135, pageEnd=1145, url=null, language=null, rfNumber=[25], rfOrder=47, authorNames=徐超华, 刘洪博, 覃伟, 毛钧, 林秀琴, 陆鑫, journalName=热带作物学报, refType=null, unstructuredReference=徐超华, 刘洪博, 覃伟, 毛钧, 林秀琴, 陆鑫. 贵州省黔西南州甘蔗野生种质资源考察收集与表型性状初步研究[J]. 热带作物学报, 2023, 44(6): 1135-1145., articleTitle=贵州省黔西南州甘蔗野生种质资源考察收集与表型性状初步研究, refAbstract=null), Reference(id=1276601978469871667, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2023, volume=44, issue=6, pageStart=1135, pageEnd=1145, url=null, language=null, rfNumber=[25], rfOrder=48, authorNames=XU C H, LIU H B, QI W, MAO J, LIN X Q, LU X, journalName=Chinese Journal of Tropical Crops, refType=null, unstructuredReference=XU C H, LIU H B, QI W, MAO J, LIN X Q, LU X. Investigation and phenotypic traits analysis of wild sugarcane germplasm resource in southwest Guizhou autonomous prefecture of China[J]. Chinese Journal of Tropical Crops, 2023, 44(6): 1135-1145. (in Chinese), articleTitle=Investigation and phenotypic traits analysis of wild sugarcane germplasm resource in southwest Guizhou autonomous prefecture of China, refAbstract=null), Reference(id=1276601978528591924, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2023, volume=54, issue=11, pageStart=3125, pageEnd=3135, url=null, language=null, rfNumber=[26], rfOrder=49, authorNames=徐超华, 陈学宽, 毛钧, 赵培方, 赵俊, 赵勇, 林秀琴, 孔春艳, 陆鑫, journalName=南方农业学报, refType=null, unstructuredReference=徐超华, 陈学宽, 毛钧, 赵培方, 赵俊, 赵勇, 林秀琴, 孔春艳, 陆鑫. 191份云南野生甘蔗种质资源采集及遗传多样性分析[J]. 南方农业学报, 2023, 54(11): 3125-3135., articleTitle=191份云南野生甘蔗种质资源采集及遗传多样性分析, refAbstract=null), Reference(id=1276601978595700789, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2023, volume=54, issue=11, pageStart=3125, pageEnd=3135, url=null, language=null, rfNumber=[26], rfOrder=50, authorNames=XU C H, CHEN X K, MAO J, ZHAO P F, ZHAO J, ZHAO Y, LIN X Q, KONG C Y, LU X, journalName=Journal of Southern Agriculture, refType=null, unstructuredReference=XU C H, CHEN X K, MAO J, ZHAO P F, ZHAO J, ZHAO Y, LIN X Q, KONG C Y, LU X. 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Genetic diversity analysis based on main phenotypic characteristics of Erianthus arundinaceum germplasm from different regions[J]. Chinese Journal of Tropical Crops, 2020, 41(6): 1108-1116. 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Phenotypic correlation and genetic diversity of decaploids of Saccharum spontaneum[J]. Journal of Hunan Agricultural University (Natural Sciences). 2012, 38(6): 574-579. (in Chinese), articleTitle=Phenotypic correlation and genetic diversity of decaploids of Saccharum spontaneum, refAbstract=null), Reference(id=1276601978964799546, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2021, volume=36, issue=4, pageStart=409, pageEnd=416, url=null, language=null, rfNumber=[29], rfOrder=55, authorNames=李德铢, 蔡杰, 贺伟, 杨湘云, journalName=中国科学院院刊, refType=null, unstructuredReference=李德铢, 蔡杰, 贺伟, 杨湘云. 野生生物种质资源保护的进展和未来设想[J]. 中国科学院院刊, 2021, 36(4): 409-416., articleTitle=野生生物种质资源保护的进展和未来设想, refAbstract=null), Reference(id=1276601979036102715, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2021, volume=36, issue=4, pageStart=409, pageEnd=416, url=null, language=null, rfNumber=[29], rfOrder=56, authorNames=LI D Z, CAI J, HE W, YANG X Y, journalName=Bulletion of Chinese Academy of Sciences, refType=null, unstructuredReference=LI D Z, CAI J, HE W, YANG X Y. Progress and prospect of wild germplasm conservation[J]. Bulletion of Chinese Academy of Sciences, 2021, 36(4): 409-416. (in Chinese), articleTitle=Progress and prospect of wild germplasm conservation, refAbstract=null), Reference(id=1276601979107405884, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2024, volume=25, issue=2, pageStart=237, pageEnd=248, url=null, language=null, rfNumber=[30], rfOrder=57, authorNames=罗嗣芳, 张祖铭, 柳嘉程, 孙静贤, 杨月华, 赖圣炜, 谢丽芳, 牛王翠, 严翔, 陈兆星, journalName=植物遗传资源学报, refType=null, unstructuredReference=罗嗣芳, 张祖铭, 柳嘉程, 孙静贤, 杨月华, 赖圣炜, 谢丽芳, 牛王翠, 严翔, 陈兆星. 赣南野生山金柑种质资源收集与鉴定[J]. 植物遗传资源学报, 2024, 25(2): 237-248., articleTitle=赣南野生山金柑种质资源收集与鉴定, refAbstract=null), Reference(id=1276601979174514749, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, doi=null, pmid=null, pmcid=null, year=2024, volume=25, issue=2, pageStart=237, pageEnd=248, url=null, language=null, rfNumber=[30], rfOrder=58, authorNames=LUO S F, ZHANG Z M, LIU J C, SUN J X, YANG Y H, LAI S W, XIE L F, NIU W C, YAN X, CHEN Z X, journalName=Journal of Plant Genetic Resources, refType=null, unstructuredReference=LUO S F, ZHANG Z M, LIU J C, SUN J X, YANG Y H, LAI S W, XIE L F, NIU W C, YAN X, CHEN Z X. Collection and identification of wild Fortunella hindsii germplasm resources in Gannan, southern Jiangxi province of China[J]. Journal of Plant Genetic Resources, 2024, 25(2): 237-248. (in Chinese), articleTitle=Collection and identification of wild Fortunella hindsii germplasm resources in Gannan, southern Jiangxi province of China, refAbstract=null)], funds=[Fund(id=1276601973264740352, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, awardId=2021YFD1200204, language=CN, fundingSource=国家重点研发计划项目(2021YFD1200204), fundOrder=null, country=null), Fund(id=1276601973331849217, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, awardId=31901510, language=CN, fundingSource=国家自然科学基金项目(31901510), fundOrder=null, country=null), Fund(id=1276601973403152386, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, awardId=桂农科2022JM10, language=CN, fundingSource=广西农业科学院科技发展基金项目(桂农科2022JM10), fundOrder=null, country=null)], companyList=[AuthorCompany(id=1276601949793416097, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, xref=1., ext=[AuthorCompanyExt(id=1276601949801804706, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, companyId=1276601949793416097, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Guangxi Key Laboratory of Sugarcane Genetic Improvement / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi 530007, China), AuthorCompanyExt(id=1276601949810193315, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, companyId=1276601949793416097, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=1.广西壮族自治区农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室/农业农村部广西甘蔗生物技术与遗传改良重点实验室,广西南宁 530007)]), AuthorCompany(id=1276601949902468004, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, xref=2., ext=[AuthorCompanyExt(id=1276601949940216741, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, companyId=1276601949902468004, language=EN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.College of Agriculture, Guangxi University, Nanning, Guangxi 530004, China), AuthorCompanyExt(id=1276601949952799654, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, companyId=1276601949902468004, language=CN, country=null, province=null, city=null, postcode=null, companyName=null, departmentName=null, remark=2.广西大学农学院,广西南宁 530004)])], figs=[ArticleFig(id=1276601971591214065, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Fig. 1, caption=Habitat images of N. porphyrocoma in Guangxi, figureFileSmall=P0yBc17sgpyjEV9rZcowxA==, figureFileBig=mH9CxJA0JdIJ9vHguqckkg==, tableContent=null), ArticleFig(id=1276601971662517234, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=图1, caption=广西河八王生境图像, figureFileSmall=P0yBc17sgpyjEV9rZcowxA==, figureFileBig=mH9CxJA0JdIJ9vHguqckkg==, tableContent=null), ArticleFig(id=1276601971817706483, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Fig. 2, caption=Cluster relationship diagram of N. porphyrocoma germplasm resources in Guangxi, figureFileSmall=pLPEBRh0F+C5Jeyi9urD+Q==, figureFileBig=qGP2PNQwioNndZJ9dVVuYg==, tableContent=null), ArticleFig(id=1276601972060976116, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=图2, caption=广西河八王种质资源聚类关系图, figureFileSmall=pLPEBRh0F+C5Jeyi9urD+Q==, figureFileBig=qGP2PNQwioNndZJ9dVVuYg==, tableContent=null), ArticleFig(id=1276601972123890677, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Tab. 1, caption=

Collection and distribution of N. porphyrocoma germplasm resources in Guangxi

, figureFileSmall=null, figureFileBig=null, tableContent=
样本编号Sample No.采集地点Collection site海拔Altitude/m经度Longitude/E纬度Latitude/N生境Habitat种质群落Germplasm community自然感病Naturally infected disease
2016HBW010贺州市富川瑶族自治县葛坡镇316.4111°17′39″25°00′14″水库旁群生轮斑
2016HBW011贺州市富川瑶族自治县麦岭镇364.2111°25′31″25°01′01″公路旁散生
2016HBW012贺州市富川瑶族自治县麦岭镇387.5111°19′44″25°05′05″公路旁散生
2016HBW013贺州市富川瑶族自治县麦岭镇377.7111°19′57″25°07′48″草地散生
2016HBW014贺州市富川瑶族自治县古城镇194.3111°20′00″24°47′12″草地散生轮斑
2016HBW015贺州市富川瑶族自治县福利镇226.0111°21′46″24°52′21″农田散生
2018HBW001柳州市鹿寨县寨沙镇125.1109°57′32″24°26′07″沟底散生轮斑
2018HBW002柳州市鹿寨县寨沙镇149.9109°56′48″24°30′44″公路旁散生轮斑
2018HBW003柳州市鹿寨县鹿寨镇124.6109°43′55″24°33′22″公路旁群生褐条
2018HBW004柳州市鹿寨县平山镇139.6109°36′27″24°40′28″公路旁散生褐条
2018HBW005柳州市鹿寨县黄冕镇111.5109°51′40″24°43′00″田埂散生眼点
2018HBW006桂林市永福县三皇镇263.5109°40′30″24°59′24″公路旁散生轮斑
2018HBW007桂林市永福县三皇镇206.8109°38′52″24°51′48″溪边散生轮斑
2018HBW008桂林市永福县罗锦镇150.3110°05′38″25°02′37″田边散生
2018HBW009桂林市永福县苏桥镇144.5110°03′48″25°05′28″田边散生褐条
2018HBW010河池市环江毛南族自治县洛阳镇228.6108°14′59″25°04′19″农田散生轮斑
2018HBW011河池市环江毛南族自治县洛阳镇243.5108°13′21″25°07′59″农田散生
2018HBW012河池市环江毛南族自治县川山镇269.3108°05′40″25°07′12″农田散生
2018HBW013柳州市融安县桥板乡602.5109°33′23″24°52′39″田间群生褐斑
2018HBW014柳州市融安县桥板乡468.4109°33′01″24°52′02″田边群生
2018HBW015柳州市融安县桥板乡453.9109°33′04″24°55′03″田间群生眼点、轮斑
2018HBW016柳州市融安县桥板乡324.6109°31′30″25°01′34″田埂群生
2018HBW017柳州市融安县泗顶镇273.4109°30′12″25°03′04″溪边群生轮斑
2018HBW018柳州市融安县长安镇119.2109°24′49″25°12′34″田埂群生轮斑
2018HBW019柳州市融安县大坡乡126.5109°27′46″25°12′09″公路旁群生
2018HBW020柳州市融安县长安镇117.9109°23′19″25°09′42″田埂群生轮斑
2018HBW021柳州市融安县浮石镇114.2109°20′25″25°05′26″公路旁群生轮斑
2018HBW022柳州市融安县大良镇206.7109°19′02″24°54′29″田埂群生
2018HBW023柳州市融安县浮石镇147.4109°41′31″25°07′40″草地散生
2018HBW024柳州市融水苗族自治县永乐镇167.3109°09′16″25°00′20″公路旁散生
2019HBW001河池市罗城仫佬族自治县东门镇316.7108°53′17″24°46′11″农田散生
2019HBW002河池市罗城仫佬族自治县小长安镇100.6109°01′29″24°53′09″农田散生
2019HBW003河池市罗城仫佬族自治县黄金镇109.8108°57′56″24°55′54″农田散生
2019HBW004河池市罗城仫佬族自治县黄金镇119.7108°57′50″24°56′11″农田散生
2019HBW005河池市罗城仫佬族自治县东门镇275.1108°54′04″24°50′34″农田群生
2019HBW006河池市罗城仫佬族自治县东门镇206.7108°54′05″24°53′00″农田散生叶焦
2019HBW007河池市罗城仫佬族自治县天河镇218.2108°42′59″24°44′29″草地散生褐条
2019HBW008河池市罗城仫佬族自治县四把镇283.8108°45′28″24°43′40″农田散生
2019HBW009柳州市柳城县冲脉镇229.0108°57′12″24°37′38″湖边群生
2019HBW010柳州市柳城县冲脉镇227.0108°57′12″24°37′38″湖边群生
2019HBW021河池市金城江区五圩镇299.1107°53′06″24°32′50″农田散生
2019HBW022河池市金城江区五圩镇332.8107°52′58″24°32′05″农田散生
2019HBW023河池市金城江区河池镇262.7107°52′15″24°39′13″草地群生褐条
2019HBW031桂林市阳朔县兴坪镇157.3110°31′57″24°53′40″农田群生
2019HBW032桂林市平乐县桥亭镇153.5110°48′22″24°31′19″农田散生
2020HBW001贵港市桂平市大湾镇39.0109°55′13″23°08′02″田间群生
2020HBW002来宾市忻城县思练镇268.0108°56′15″24°02′44″田间散生
2020HBW005桂林市兴安县崔家乡248.1110°40′33″25°32′00″农田群生
2020HBW006桂林市兴安县高尚镇258.9110°35′25″25°26′21″农田群生轮斑
2020HBW007桂林市兴安县严关镇197.1110°32′07″25°36′30″农田群生
2020HBW008桂林市全州县全州镇158.2111°01′38″25°57′27″农田群生白疹
2020HBW009桂林市全州县龙水镇174.7110°59′15″26°01′43″农田群生
2020HBW010桂林市全州县凤凰镇175.1110°54′09″25°49′22″农田群生
2020HBW011贺州市钟山县钟山镇148.8111°17′14″24°37′03″农田群生
2020HBW012贺州市钟山县燕塘镇192.2111°08′34″24°29′47″农田群生
2020HBW013梧州市苍梧县沙头镇60.0111°32′02″23°54′39″农田群生
2023HBW022百色市田东县印茶镇358.0107°08′01″23°21′34″公路旁散生
2023HBW023百色市田东县江城镇288.0107°09′22″23°21′44″公路旁散生
), ArticleFig(id=1276601972274885622, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=表1, caption=

广西河八王种质资源采集和分布概况

, figureFileSmall=null, figureFileBig=null, tableContent=
样本编号Sample No.采集地点Collection site海拔Altitude/m经度Longitude/E纬度Latitude/N生境Habitat种质群落Germplasm community自然感病Naturally infected disease
2016HBW010贺州市富川瑶族自治县葛坡镇316.4111°17′39″25°00′14″水库旁群生轮斑
2016HBW011贺州市富川瑶族自治县麦岭镇364.2111°25′31″25°01′01″公路旁散生
2016HBW012贺州市富川瑶族自治县麦岭镇387.5111°19′44″25°05′05″公路旁散生
2016HBW013贺州市富川瑶族自治县麦岭镇377.7111°19′57″25°07′48″草地散生
2016HBW014贺州市富川瑶族自治县古城镇194.3111°20′00″24°47′12″草地散生轮斑
2016HBW015贺州市富川瑶族自治县福利镇226.0111°21′46″24°52′21″农田散生
2018HBW001柳州市鹿寨县寨沙镇125.1109°57′32″24°26′07″沟底散生轮斑
2018HBW002柳州市鹿寨县寨沙镇149.9109°56′48″24°30′44″公路旁散生轮斑
2018HBW003柳州市鹿寨县鹿寨镇124.6109°43′55″24°33′22″公路旁群生褐条
2018HBW004柳州市鹿寨县平山镇139.6109°36′27″24°40′28″公路旁散生褐条
2018HBW005柳州市鹿寨县黄冕镇111.5109°51′40″24°43′00″田埂散生眼点
2018HBW006桂林市永福县三皇镇263.5109°40′30″24°59′24″公路旁散生轮斑
2018HBW007桂林市永福县三皇镇206.8109°38′52″24°51′48″溪边散生轮斑
2018HBW008桂林市永福县罗锦镇150.3110°05′38″25°02′37″田边散生
2018HBW009桂林市永福县苏桥镇144.5110°03′48″25°05′28″田边散生褐条
2018HBW010河池市环江毛南族自治县洛阳镇228.6108°14′59″25°04′19″农田散生轮斑
2018HBW011河池市环江毛南族自治县洛阳镇243.5108°13′21″25°07′59″农田散生
2018HBW012河池市环江毛南族自治县川山镇269.3108°05′40″25°07′12″农田散生
2018HBW013柳州市融安县桥板乡602.5109°33′23″24°52′39″田间群生褐斑
2018HBW014柳州市融安县桥板乡468.4109°33′01″24°52′02″田边群生
2018HBW015柳州市融安县桥板乡453.9109°33′04″24°55′03″田间群生眼点、轮斑
2018HBW016柳州市融安县桥板乡324.6109°31′30″25°01′34″田埂群生
2018HBW017柳州市融安县泗顶镇273.4109°30′12″25°03′04″溪边群生轮斑
2018HBW018柳州市融安县长安镇119.2109°24′49″25°12′34″田埂群生轮斑
2018HBW019柳州市融安县大坡乡126.5109°27′46″25°12′09″公路旁群生
2018HBW020柳州市融安县长安镇117.9109°23′19″25°09′42″田埂群生轮斑
2018HBW021柳州市融安县浮石镇114.2109°20′25″25°05′26″公路旁群生轮斑
2018HBW022柳州市融安县大良镇206.7109°19′02″24°54′29″田埂群生
2018HBW023柳州市融安县浮石镇147.4109°41′31″25°07′40″草地散生
2018HBW024柳州市融水苗族自治县永乐镇167.3109°09′16″25°00′20″公路旁散生
2019HBW001河池市罗城仫佬族自治县东门镇316.7108°53′17″24°46′11″农田散生
2019HBW002河池市罗城仫佬族自治县小长安镇100.6109°01′29″24°53′09″农田散生
2019HBW003河池市罗城仫佬族自治县黄金镇109.8108°57′56″24°55′54″农田散生
2019HBW004河池市罗城仫佬族自治县黄金镇119.7108°57′50″24°56′11″农田散生
2019HBW005河池市罗城仫佬族自治县东门镇275.1108°54′04″24°50′34″农田群生
2019HBW006河池市罗城仫佬族自治县东门镇206.7108°54′05″24°53′00″农田散生叶焦
2019HBW007河池市罗城仫佬族自治县天河镇218.2108°42′59″24°44′29″草地散生褐条
2019HBW008河池市罗城仫佬族自治县四把镇283.8108°45′28″24°43′40″农田散生
2019HBW009柳州市柳城县冲脉镇229.0108°57′12″24°37′38″湖边群生
2019HBW010柳州市柳城县冲脉镇227.0108°57′12″24°37′38″湖边群生
2019HBW021河池市金城江区五圩镇299.1107°53′06″24°32′50″农田散生
2019HBW022河池市金城江区五圩镇332.8107°52′58″24°32′05″农田散生
2019HBW023河池市金城江区河池镇262.7107°52′15″24°39′13″草地群生褐条
2019HBW031桂林市阳朔县兴坪镇157.3110°31′57″24°53′40″农田群生
2019HBW032桂林市平乐县桥亭镇153.5110°48′22″24°31′19″农田散生
2020HBW001贵港市桂平市大湾镇39.0109°55′13″23°08′02″田间群生
2020HBW002来宾市忻城县思练镇268.0108°56′15″24°02′44″田间散生
2020HBW005桂林市兴安县崔家乡248.1110°40′33″25°32′00″农田群生
2020HBW006桂林市兴安县高尚镇258.9110°35′25″25°26′21″农田群生轮斑
2020HBW007桂林市兴安县严关镇197.1110°32′07″25°36′30″农田群生
2020HBW008桂林市全州县全州镇158.2111°01′38″25°57′27″农田群生白疹
2020HBW009桂林市全州县龙水镇174.7110°59′15″26°01′43″农田群生
2020HBW010桂林市全州县凤凰镇175.1110°54′09″25°49′22″农田群生
2020HBW011贺州市钟山县钟山镇148.8111°17′14″24°37′03″农田群生
2020HBW012贺州市钟山县燕塘镇192.2111°08′34″24°29′47″农田群生
2020HBW013梧州市苍梧县沙头镇60.0111°32′02″23°54′39″农田群生
2023HBW022百色市田东县印茶镇358.0107°08′01″23°21′34″公路旁散生
2023HBW023百色市田东县江城镇288.0107°09′22″23°21′44″公路旁散生
), ArticleFig(id=1276601972367160311, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Tab. 2, caption=

Genetic variation analysis of quantitative traits of N. porphyrocoma germplasm resources in Guangxi

, figureFileSmall=null, figureFileBig=null, tableContent=
性状Traits最小值Min最大值Max均值Mean标准差SD变异系数CV/%
叶长/cm68.00145.20110.1519.080±2.50517.3
叶宽/cm0.654.401.510.618±0.08140.9
株高/cm61.00298.00153.8153.745±7.05734.9
茎径/cm0.121.280.680.192±0.02528.3
节间长度/cm7.3061.0023.7312.720±1.67053.6
锤度/%3.0018.8011.383.808±0.50033.5
), ArticleFig(id=1276601972434269176, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=表2, caption=

广西河八王种质资源数量性状遗传变异分析

, figureFileSmall=null, figureFileBig=null, tableContent=
性状Traits最小值Min最大值Max均值Mean标准差SD变异系数CV/%
叶长/cm68.00145.20110.1519.080±2.50517.3
叶宽/cm0.654.401.510.618±0.08140.9
株高/cm61.00298.00153.8153.745±7.05734.9
茎径/cm0.121.280.680.192±0.02528.3
节间长度/cm7.3061.0023.7312.720±1.67053.6
锤度/%3.0018.8011.383.808±0.50033.5
), ArticleFig(id=1276601972505572345, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Tab. 3, caption=

Genetic variation analysis of quality traits of N. porphyrocoma germplasm resources in Guangxi

, figureFileSmall=null, figureFileBig=null, tableContent=
性状Trait分级频率Frequency/%H
012345678
花序颜色6.93.441.448.31.0160
花序形状6.991.41.70.3359
蒲心15.51.76.975.90.7520
57号毛群25.98.651.713.81.1752
空心60.339.70.6717
蜡粉带50.048.31.70.7673
气根100.00.0000
茎形100.00.0000
木栓98.31.70.0861
根点排列88.010.31.70.4158
芽沟81.110.38.60.6150
生长带形状13.886.20.4013
脱叶性3.496.60.1483
水裂100.00.0000
叶姿31.056.912.10.9394
芽形86.28.65.20.4927
曝光前节间颜色46.636.217.21.0264
曝光后节间颜色19.058.65.28.68.61.2044
芽位56.922.420.70.9820
节间形状98.31.70.0861
), ArticleFig(id=1276601972627207162, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=表3, caption=

广西河八王种质资源质量性状遗传变异分析

, figureFileSmall=null, figureFileBig=null, tableContent=
性状Trait分级频率Frequency/%H
012345678
花序颜色6.93.441.448.31.0160
花序形状6.991.41.70.3359
蒲心15.51.76.975.90.7520
57号毛群25.98.651.713.81.1752
空心60.339.70.6717
蜡粉带50.048.31.70.7673
气根100.00.0000
茎形100.00.0000
木栓98.31.70.0861
根点排列88.010.31.70.4158
芽沟81.110.38.60.6150
生长带形状13.886.20.4013
脱叶性3.496.60.1483
水裂100.00.0000
叶姿31.056.912.10.9394
芽形86.28.65.20.4927
曝光前节间颜色46.636.217.21.0264
曝光后节间颜色19.058.65.28.68.61.2044
芽位56.922.420.70.9820
节间形状98.31.70.0861
), ArticleFig(id=1276601972702704635, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Tab. 4, caption=

Shannon-Wiener diversity index of N. porphyrocoma germplasm resources from different collection regions of Guangxi

, figureFileSmall=null, figureFileBig=null, tableContent=
性状TraitH
桂林Guilin柳州Liuzhou贺州Hezhou河池Hechi百色Baise
花序颜色0.56230.87851.08210.65170
花序形状000.974300
蒲心0.23580.40940.37670.89820
57号毛群0.53761.43631.32080.65590
空心0.32391.13710.56230.68290.6931
蜡粉带0.82390.69170.69310.41010
气根00000
茎形00000
木栓0.20700000
根点排列00.53660.562300.6931
芽沟0.867500.73560.89210
生长带形状0.36620000
脱叶性0.20700000
水裂00000
叶姿0.95960.70800.66150.59820.6931
芽形0.86750.33640.37670.25730
曝光前节间颜色0.88761.01980.56230.75890
曝光后节间颜色1.24241.05960.56230.89210
芽位0.72140.91740.97430.65170
节间形状00.20610.376700
平均值0.44050.46680.49110.36750.1040
), ArticleFig(id=1276601972803367932, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=表4, caption=

不同采集地区广西河八王种质资源Shannon-Wiener多样性指数

, figureFileSmall=null, figureFileBig=null, tableContent=
性状TraitH
桂林Guilin柳州Liuzhou贺州Hezhou河池Hechi百色Baise
花序颜色0.56230.87851.08210.65170
花序形状000.974300
蒲心0.23580.40940.37670.89820
57号毛群0.53761.43631.32080.65590
空心0.32391.13710.56230.68290.6931
蜡粉带0.82390.69170.69310.41010
气根00000
茎形00000
木栓0.20700000
根点排列00.53660.562300.6931
芽沟0.867500.73560.89210
生长带形状0.36620000
脱叶性0.20700000
水裂00000
叶姿0.95960.70800.66150.59820.6931
芽形0.86750.33640.37670.25730
曝光前节间颜色0.88761.01980.56230.75890
曝光后节间颜色1.24241.05960.56230.89210
芽位0.72140.91740.97430.65170
节间形状00.20610.376700
平均值0.44050.46680.49110.36750.1040
), ArticleFig(id=1276601972874671101, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Tab. 5, caption=

Comparison among N. porphyrocoma germplasm resources from different collection regions of Guangxi

, figureFileSmall=null, figureFileBig=null, tableContent=
采集地区Collection region叶长Leaf length叶宽Leaf width株高Plant height茎径Stalk diameter节间长度Internode length锤度Brix
均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%
桂林117.1816.21.3331.3164.5825.00.6419.424.1250.611.2534.1
柳州101.4517.91.3635.3161.1127.00.7027.425.1663.511.7230.8
贺州112.7318.61.6433.7144.1340.70.7815.524.6637.311.6836.0
河池113.4316.41.7652.0141.8441.90.5532.421.4552.912.4525.5
百色101.5017.42.0014.1148.5039.50.5129.218.2529.16.0070.7
), ArticleFig(id=1276601972958557182, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=表5, caption=

不同采集地区广西河八王种质资源差异比较

, figureFileSmall=null, figureFileBig=null, tableContent=
采集地区Collection region叶长Leaf length叶宽Leaf width株高Plant height茎径Stalk diameter节间长度Internode length锤度Brix
均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%均值Mean/cm变异系数CV/%
桂林117.1816.21.3331.3164.5825.00.6419.424.1250.611.2534.1
柳州101.4517.91.3635.3161.1127.00.7027.425.1663.511.7230.8
贺州112.7318.61.6433.7144.1340.70.7815.524.6637.311.6836.0
河池113.4316.41.7652.0141.8441.90.5532.421.4552.912.4525.5
百色101.5017.42.0014.1148.5039.50.5129.218.2529.16.0070.7
), ArticleFig(id=1276601973038248959, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=EN, label=Tab. 6, caption=

Analysis of group characteristics of N. porphyrocoma germplasm resources in Guangxi

, figureFileSmall=null, figureFileBig=null, tableContent=
种质Germplasm类群Group数量Number叶长Leaf length/cm叶宽Leaf width/cm株高Plant height/cm茎径Stalk diameter/cm节间长度Internode length/cm锤度Brix/%
河八王47109.101.53159.350.6424.6910.42
1108.002.22254.000.7817.5018.80
7102.071.29143.110.5724.0712.06
1118.000.90161.000.8030.0012.00
1136.001.62238.000.8012.508.80
198.504.4061.000.4812.0011.50
), ArticleFig(id=1276601973113746432, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601400691921445, language=CN, label=表6, caption=

广西河八王种质资源类群特征分析

, figureFileSmall=null, figureFileBig=null, tableContent=
种质Germplasm类群Group数量Number叶长Leaf length/cm叶宽Leaf width/cm株高Plant height/cm茎径Stalk diameter/cm节间长度Internode length/cm锤度Brix/%
河八王47109.101.53159.350.6424.6910.42
1108.002.22254.000.7817.5018.80
7102.071.29143.110.5724.0712.06
1118.000.90161.000.8030.0012.00
1136.001.62238.000.8012.508.80
198.504.4061.000.4812.0011.50
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广西河八王种质资源考察收集与鉴定评价
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宗文一 1, 2 , 张保青 1 , 张革民 1 , 黄玉新 1 , 杨翠芳 1 , 周珊 1 , 高轶静 1 , 熊发前 1 , 刘菁 1 , 陆衫羽 1 , 杨细平 2, * , 段维兴 1, *
热带作物学报 | 种质资源与遗传育种 2025,46(1): 94-105
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热带作物学报 |种质资源与遗传育种 2025 , 46 (1) : 94 -105
广西河八王种质资源考察收集与鉴定评价
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宗文一1, 2, 张保青1, 张革民1, 黄玉新1, 杨翠芳1, 周珊1, 高轶静1, 熊发前1, 刘菁1, 陆衫羽1, 杨细平2, * , 段维兴1, *
作者信息
  • 1.广西壮族自治区农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室/农业农村部广西甘蔗生物技术与遗传改良重点实验室,广西南宁 530007
  • 2.广西大学农学院,广西南宁 530004
通讯作者:
* 杨细平(YANG Xiping),E-mail:
段维兴(DUAN Weixing),E-mail:
Collection, Identification and Evaluation of Narenga porphyrocoma Germplasm Resources in Guangxi
Wenyi ZONG1, 2, Baoqing ZHANG1, Gemin ZHANG1, Yuxin HUANG1, Cuifang YANG1, Shan ZHOU1, Yijing GAO1, Faqian XIONG1, Jing LIU1, Shanyu LU1, Xiping YANG2, * , Weixing DUAN1, *
Affiliations
  • 1.Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences / Guangxi Key Laboratory of Sugarcane Genetic Improvement / Key Laboratory of Sugarcane Biotechnology and Genetic Improvement (Guangxi), Ministry of Agriculture and Rural Affairs, Nanning, Guangxi 530007, China
  • 2.College of Agriculture, Guangxi University, Nanning, Guangxi 530004, China
出版时间: 2025-01-25 doi: 10.3969/j.issn.1000-2561.2025.01.010
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广西地跨南亚热带和中亚热带,北回归线横贯中部,属亚热带季风气候区,孕育了丰富的野生甘蔗种质资源。2016—2023年,广西农业科学院甘蔗研究所组织考察队对广西河八王种质资源开展系统调查和收集工作,考察队在广西8个地级市18个县(市、区)43个乡镇共收集到河八王种质资源58份。调查结果表明:广西河八王种质资源主要分布在海拔60.0~602.5 m,北纬23°08′02″~26°01′43″,东经107°08′01″~111°32′02″的区域;广西河八王种质资源20个描述型质量性状的遗传多样性指数在0~1.2044之间,平均值为0.5557,其中曝光后节间颜色的多样性最高,木栓较低,而茎形、气根、水裂3个性状无多样性体现;不同收集地区的广西河八王种质资源遗传多样性指数在0.1040~0.4911之间,贺州最高,百色最低;广西河八王种质资源表型性状变异丰富,6个数量性状的变异系数为17.3%~53.6%,平均为34.8%;不同收集地区的广西河八王种质资源变异系数在29.4%~36.9%之间,河池最大,桂林最小。聚类分析结果显示,58份广西河八王种质资源被分为六大类,与地理来源无密切关系,其中第Ⅰ类群中的材料综合性状较好,生物量和品质性状优良。本次考察收集扩充了广西甘蔗种质资源圃的资源数量,为甘蔗育种和遗传改良提供了优良基因资源。

河八王  /  甘蔗  /  种质资源  /  表型性状  /  鉴定评价  /  遗传多样性

Guangxi spans the southern subtropics and central subtropics, with the Tropic of Cancer crossing the central part of the province, and belongs to the subtropical monsoon climate zone, which breeds rich wild sugarcane germplasm resources. From 2016 to 2023, Sugarcane Research Institute, Guangxi Academy of Agricultural Sciences organized a team of researchers to carry out systematic surveys and collection of Narenga porphyrocoma germplasm resources in Guangxi. The team collected a total of 58 copies of N. porphyrocoma germplasm resources in 43 townships of 18 cities and districts in Guangxi. The results showed that the N. porphyrocoma germplasm resources in Guangxi were mainly distributed in the area of 60.0-602.5 m above sea level, longitude E: 107°08′01″-111°32′02″, latitude N: 23°08′02″-26°01′43″. The genetic diversity indices of the 20 descriptive quality traits of N. porphyrocoma germplasm resources in Guangxi ranged from 0 to 1.2044, with a mean value of 0.5557, in which the diversity of the color of the internodes after exposure was the highest, that of the cork was lower, and the diversity of the three traits of stem shape, aerial roots and water splitting was not reflected; the genetic diversity indices of N. porphyrocoma germplasm resources in Guangxi of the different collection areas ranged from 0.1040 to 0.4911, with Hezhou being the highest and Baise being the lowest. The phenotypic traits of N. porphyrocoma germplasm resources in Guangxi were rich in variation, and the coefficients of variation for six quantitative traits ranged from 17.3% to 53.6%, with an average of 34.8%; the coefficients of variation of N. porphyrocoma germplasm resources in Guangxi from different collection areas ranged from 29.4% to 36.9%, with Hechi being the largest and Guilin being the smallest. The results of cluster analysis showed that the 58 N. porphyrocoma germplasm resources in Guangxi were classified into six major groups, but were not closely related to the geographical origin, in which the materials in the first major group had better overall traits and excellent biomass and quality traits. The collection expanded the number of resources in Guangxi Sugarcane Germplasm Repository and would provide excellent genetic resources for sugarcane breeding and genetic improvement.

Narenga porphyrocoma  /  sugarcane  /  germplasm resources  /  phenotypic traits  /  identification and evaluation  /  genetic diversity
宗文一, 张保青, 张革民, 黄玉新, 杨翠芳, 周珊, 高轶静, 熊发前, 刘菁, 陆衫羽, 杨细平, 段维兴. 广西河八王种质资源考察收集与鉴定评价. 热带作物学报, 2025 , 46 (1) : 94 -105 . DOI: 10.3969/j.issn.1000-2561.2025.01.010
Wenyi ZONG, Baoqing ZHANG, Gemin ZHANG, Yuxin HUANG, Cuifang YANG, Shan ZHOU, Yijing GAO, Faqian XIONG, Jing LIU, Shanyu LU, Xiping YANG, Weixing DUAN. Collection, Identification and Evaluation of Narenga porphyrocoma Germplasm Resources in Guangxi[J]. Chinese Journal of Tropical Crops, 2025 , 46 (1) : 94 -105 . DOI: 10.3969/j.issn.1000-2561.2025.01.010
种质资源是甘蔗育种的物质基础,甘蔗种质改良和突破性育种所取得的巨大成就主要依赖于种质资源的发掘和利用程度[1],大量甘蔗种质材料的保育、开发研究为培育新的优异商业品种提供了良好的遗传材料。河八王[Narenga porphyrocoma(Hance)Bor]又名草鞋密,是禾本科甘蔗亚族河八王属的多年生植物,染色体数2n=30,主要分布在我国江苏、江西、广东、广西、四川等省(区)[2],具有耐旱耐瘠、分蘖力强、抗黑穗病、抗赤腐病等优良性状,是甘蔗抗黑穗病育种良好基因源材料。河八王须根坚韧粗壮,具有防风固沙和减少水土流失的作用[3]。河八王具有优秀的固氮能力,是氮高效利用的重要材料[4]。河八王纤维素含量高,生物质产量大,具有较高的生物质能转化潜力,是一种潜在的能源植物[5]。李海碧等[6]通过对河八王转录组研究发现,河八王转录组具有丰富的SSR和SNP位点,具有较高的遗传多态性。刘昔辉等[7]利用AFLP标记对15份广西河八王进行遗传多样性分析,结果显示河八王无性系的聚类与地域有一定程度的关系。段维兴等[8-10]通过甘蔗与河八王杂交获得F1和BC1代的抗黑穗病甘蔗亲本,探明了杂交后代F1、BC1、BC2的染色体遗传方式。以上研究表明,河八王相比其他甘蔗近缘属植物具有更高的利用潜力,遗传多样性丰富,是一种具有极高生态和经济价值的生物质资源。
广西地处中国地势第二级阶梯中的云贵高原东南边缘,地势由西北向东南倾斜,四周山地围绕,呈盆地状,也是中国降水量最丰富的地区之一,年降水量均在1070 mm以上,年均气温16.0~23.0 ℃[11-12],属亚热带季风气候和热带季风气候,孕育了丰富的甘蔗野生种质资源[13]。为探明广西河八王种质资源分布概况,丰富广西甘蔗种质资源圃的资源数量与遗传多样性,广西农业科学院甘蔗研究所组织考察队在广西开展河八王种质资源的系统调查与采集,对其主要表型性状的相关性和遗传多样性进行分析,以期挖掘到具有育种潜力的种质材料,为甘蔗育种和遗传改良提供优异亲本。
2016—2023年,广西农业科学院甘蔗研究所组织考察队在广西全区范围内14个地级市75个县(市、区)开展甘蔗种质资源系统调查与收集。资源采集方法:运用GPS仪记录采集资源的经纬度、海拔等地理信息。在调查采集过程中,填写《甘蔗及其近缘属野生种质资源调查采集表》,包括采集日期、采集人、采集编号、种质类型、采集地点、地理信息、种质群落、伴生植物、土壤类型、物候期等信息。采集植株特性图片,挖掘带土的地下根茎,与带采集编号的标签一起放入样品袋。每隔2 d将样品邮寄回广西甘蔗种质资源圃,并种植在野生资源保育水泥框(直径×深度=70 cm×60 cm)内。
在河八王资源开花期调查表型性状,随机选取5条测定其数量性状和质量性状,测定方法参照《甘蔗种质资源描述规范》[14]。6个数量性状包括正1叶长度、正1叶宽度、株高、节间长度、茎径、锤度。使用卷尺测量正1叶的叶长和叶宽,节间长度;使用塔尺测量株高;使用游标卡尺测量茎径;使用锤度计测量锤度。质量性状包括蒲心、空心、57号毛群、蜡粉带、茎形等20个性状。
运用Excel 2019软件对调查数据进行整理与分类。采用SPSS 26软件对数量性状进行变异系数(CV)分析,描述6个数量性状的离散程度,采用Shannon-Wiener多样性指数(Shannon-Wiener diversity index,H′)进行遗传多样性评价,其计算公式H′=-ΣPiLnPi,式中Pi为某性状第i个代码值出现的频率,Ln表示自然对数[15]。使用DPS 9.01软件采用非加权配对算术平均法绘制聚类关系图,并使用MEGA 11软件对聚类关系图进行美化。
经考察和收集,在广西贺州市富川瑶族自治县、钟山县,柳州市鹿寨县、融安县、融水苗族自治县、柳城县,河池市环江毛南族自治县、罗城仫佬族自治县、金城江区,桂林市永福县、阳朔县、平乐县、兴安县、全州县,梧州市苍梧县,贵港市桂平市,来宾市忻城县,百色市田东县等18个县(市、区)43个乡镇采集到58份河八王种质资源(表1)。地理水平分布表明,广西河八王种质资源主要分布在海拔60.0~602.5 m,北纬23°08′02″~26°01′43″,东经107°08′01″~111°32′02″。采集地生态环境主要在沼泽地、公路旁、草地、田间(图1)。在自然条件下,广西河八王叶片存在一定程度的感病现象,主要为轮斑病、褐条病、眼点、叶焦和白疹等。
数量性状分析结果显示(表2),广西河八王种质资源的数量性状存在较为丰富的遗传变异。叶长最高为145.20 cm,最低为68.00 cm,平均为110.15 cm;叶宽最高为4.40 cm,最低为0.65 cm,平均为1.51 cm;株高最高为298.00 cm,最低为61.00 cm,平均为153.81 cm;茎径最高为1.28 cm,最低为0.12 cm,平均为0.68 cm;节间长度最高为61.00 cm,最低为7.30 cm,平均为23.73 cm;锤度(Bx)最高为18.80%,最低为3.00%,平均11.38%。6个数量性状的平均变异系数为34.8%,变异幅度为17.3%~53.6%,其中株高、叶宽、节间长度、锤度具有较大的离散程度,节间长度的变异系数最高(53.6%),叶长的变异系数最低(17.3%)。结果表明,河八王群体数量性状的遗传变异主要来自于株高、叶宽、节间长度和锤度,而叶长和茎径2个性状相对稳定。
表3可知,58份广西河八王种质资源质量性状的Shannon-Wiener多样性指数在0~1.2044之间,平均值为0.5557。以曝光后节间颜色的多样性最高(1.2044),多样性较丰富;57号毛群(1.1752)、曝光前节间颜色(1.0264)、花序颜色(1.0160)、芽位(0.9820)、叶姿(0.9394)、蜡粉带(0.7673)、蒲心(0.7520)、空心(0.6717)、芽沟(0.6150)等的多样性中等;芽形、根点排列、生长带形状、花序形状、脱叶性、节间形状和木栓的多样性水平较低;茎形、气根、水裂等3个性状无多样性体现。总体而言,广西河八王材料的类型主要为无气根,茎形直立,无水裂,大部分材料表现为无木栓(98.3%),圆筒形节间(98.3%)、难脱叶(96.6%)、生长带不突出(86.2%),无根点(88.0%),无空心(60.3%),重度蒲心(75.9%),无蜡粉带(50.0%),曝光前节间为黄色(46.6%),曝光后节间为黄绿色(58.6%)。芽形以三角形(86.2%)为主,上芽位(56.9%),无芽沟(81.1%)。叶姿挺直叶尖下垂(56.9%),花序形状以圆锥形(91.4%)为主,颜色以淡紫(41.4%)和紫红(48.3%)为主,部分材料未开花。除部分材料无57号毛群外,其余多数材料都有,其中8.6%毛群少,51.7%毛群多,13.8%毛群较多。
对不同收集地区的广西河八王种质资源遗传多样性进行比较发现(表4),除来宾、贵港、梧州的样本量较小外,其余地区柳州河八王和贺州河八王以57号毛群的多样性最高,桂林河八王以曝光后节间颜色的多样性最高,河池河八王以蒲心的多样性最高,百色河八王以空心、根点排列和叶姿的多样性最高。而花序形状仅在贺州河八王中存在多样性,木栓仅在桂林河八王中存在多样性。5个收集地区河八王平均多样性指数在0.1040~0.4911之间,且以贺州的多样性最大,百色的多样性最小,其次依次为柳州(0.4668)>桂林(0.4405)>河池(0.3675)。
对不同采集地区广西河八王种质资源的6个数量性状进行比较可知(表5),桂林河八王平均株高(164.58 cm)和平均叶长(117.18 cm)最大,平均叶宽(1.33 cm)最低;柳州河八王平均节间长度(25.16 cm)最大,平均叶长(101.45 cm)最低;河池河八王平均锤度(12.45%)最高;百色河八王平均叶宽(2.00 cm)最高,平均锤度(6.00%)和平均节间长度(18.25 cm)最低。从不同收集地区6个数量性状的变异系数来看,叶长的变异系数以贺州(18.6%)的最大,桂林的(16.2%)最小;叶宽的变异系数以河池(52.0%)的最大,百色(14.1%)的最小;株高的变异系数以河池(41.9%)的最大,桂林(25.0%)的最小;茎径的变异系数以河池(32.4%)的最大,贺州的最小(15.5%);节间长度的变异系数以柳州(63.5%)的最大,百色(29.1%)的最小;锤度的变异系数以百色(70.7%)的最大,河池的最小(25.5%)。整体而言,桂林河八王的节间长度具有较大的遗传差异,叶长的遗传差异最低但平均叶长最高;柳州河八王除叶长外其余4个数量性状平均值与桂林河八王差异不大,除锤度外,其他5个数量性状的变异程度均比桂林河八王高;贺州河八王6个数量性状平均值均处于中间水平,叶长的遗传差异最高;河池河八王株高较低,节间长度变异系数大,但总体偏低;百色河八王锤度低但有较大的遗传差异,叶片较宽但遗传差异较小,节间较短且遗传差异也较小。
基于6个数量性状,对58份广西河八王种质资源进行聚类分析(图2)。结果显示,58份广西河八王材料之间的遗传距离变幅为0.70~5.74,其中,2018HBW019与2018HBW020之间的遗传距离最小(0.70),2016HBW010与2019HBW021之间的遗传距离最大(5.74)。当遗传距离为3.44时,58份广西河八王材料被划分为六大类群(表6)。第Ⅰ类群包含47份河八王材料,其中2份来自百色,1份来自贵港,12份来自桂林,14份来自河池,8份来自贺州,1份来自来宾,9份来自柳州。从类群特征上分析,第Ⅰ类群在全部类群中的节间长度和叶长相对较长,分别为24.69、109.10 cm。第Ⅱ类群仅包含1份来自柳州的河八王材料,第Ⅱ类群在全部类群中的株高和锤度最高,分别为254 cm和18.80%,叶宽相对较长,为2.22 cm。第Ⅲ类群包含7份河八王资源,均来自柳州,第Ⅲ类群在全部类群中节间长度及锤度相对较高,分别为24.07 cm和12.06%,茎径最细,为0.57 cm。第Ⅳ、Ⅴ、Ⅵ类群均只包含1份河八王资源,其中第Ⅳ、Ⅴ类群的河八王资源来自柳州,第Ⅵ类群的来自梧州,第Ⅳ、Ⅴ类群在全部的河八王材料中茎径最粗,为0.80 cm,第Ⅳ类群在全部的河八王材料中节间长度最长,为30.00 cm,第Ⅴ类群在全部的河八王材料中叶长最长,为136.00 cm,锤度最低,为8.80%,第Ⅵ类群在全部的河八王材料中叶宽最大,为4.40 cm,株高最低,为61.00 cm。聚类结果表明,材料的类群划分与地理分布无明显联系,但也有少数来源相同的材料优先聚在一起,如第Ⅲ类的7份材料均来自柳州。第Ⅱ、Ⅳ、Ⅴ、Ⅵ类群由于仅包含1份材料,参考价值低,对比第Ⅰ类群和第Ⅲ类群,除锤度外,第Ⅰ类群均高于第Ⅲ类群,综合性状较好,但其他四大类群材料在一些数量性状上均有明显的优势,有一定的育种利用价值,可作为特殊材料进行甘蔗育种。
遗传多样性与环境多样性共同决定了植物表型性状多样性,对表型性状的研究可以从整体上了解研究对象的多样性程度[16]。近年来,育种进程的加速使甘蔗杂交育种集中使用少数性状优良的亲本,从而大大降低了甘蔗种质基因的遗传多样性,导致新培育的甘蔗品种在有利性状上的变异幅度降低,甚至可能已达到了某种程度的极限[17-19]。甘蔗种质资源不仅是甘蔗原始科技创新和现代甘蔗种业发展的物质基础,也是保障国家食糖安全、促进西南地区农民增收及支撑蔗糖产业可持续发展的战略性资源[20]。调查和分析甘蔗种质资源的表型性状,既是种质资源研究的首要工作,也是甘蔗育种的基础性工作[21]。河八王属包含2个种,金猫尾[Narenga fallax(Balansa)Bor.]与河八王,此次收集的58份河八王资源分布在广西8个地级市18个县(市、区)43个乡镇,蔡泽霖等[13]在1979—1985年间调查广西野生甘蔗资源,除桂林市外,其余地级市均采集到河八王种质资源,在南宁、百色、钦州气候适宜雨水充足地区生长繁茂,而本次采集仅在贺州市、柳州市、河池市、桂林市、梧州市、贵港市、来宾市、百色市采集到河八王,原因可能是随着时间的推移,人类活动范围增加导致部分河八王生境被破坏。多数河八王种质资源生长于路边和农田,这与吴建明等[22]的研究结果一致。杜卫红等[23]通过分析表明在众多环境因素中海拔对河八王的生长影响较大,海拔600 m范围以内适宜河八王生长。本次考察采集的河八王种质资源均来自海拔600 m范围以内地区,与上述研究结果一致。58份广西河八王种质资源质量性状的Shannon-Wiener多样性指数在0~1.2044之间,6个数量性状的平均变异系数为34.8%,变异幅度为17.3%~53.6%,表明具有丰富的遗传多样性,与刘昔辉等[7]的分析结果相近。
本研究对广西区内不同采集地区的河八王种质资源表型差异进行比较表明,在柳州市采集的河八王材料大多属于高大型,而在河池市采集的河八王材料多为矮小型;在贺州市采集的河八王材料质量性状多样性丰富,在河池市采集的河八王材料数量性状变异大。这与黄玉新等[24]对广西斑茅的研究结果不同,原因可能是不同类型的野生甘蔗种质资源适宜气候和适宜分布范围有差异,导致在不同地区采集的河八王资源在株高、锤度、茎径等指标上出现差异,产生不同程度的变异从而适应不同的环境。本次采集的河八王种质资源与徐超华等[25-26]在贵州省黔西南州及云南采集的甘蔗野生种质资源相比,在节间长度和锤度等性状有较大的优势,在叶长、叶宽、株高、茎径等性状上各有优势。本次采集的河八王种质资源6个数量性状的平均变异系数为34.8%,变异幅度为17.3%~53.6%,与王春芳等[27]在云南、四川、江西、广东、福建、海南、西藏等省(区)采集的斑茅种质资源相比,叶长、叶宽、茎径、节间长度、锤度等5个数量性状的变异系数更高,同时平均变异系数高于云南、四川、江西、广东、海南、西藏等省份,表明本次采集的河八王种质资源存在较丰富的遗传多样性。
本研究的聚类结果表明,材料的划分与地理来源无明显的联系,但也有少部分来源相同的材料优先聚在一起,这与刘昔辉等[7]的研究结果不同,但与刘新龙等[28]的研究结果基本相同,原因在于本研究是基于河八王表型性状数据进行聚类分析,而刘昔辉等[7]是以AFLP标记基因型数据进行聚类分析,从而导致结果上的差异。另外,在河八王资源采集过程中,发现2018HBW019和2019HBW023两份材料的锤度很高,分别达到了18.80%和18.50%,这在以往的河八王资源采集中很少见。本研究通过对采集到的河八王种质资源进行表型性状评价,已初步筛选出一批综合性状优良、有育种价值的优异资源,但尚未进一步开展分子水平和基因水平的优异基因挖掘。
随着人类活动愈发深入,全球气候发生变化,许多野生资源的原生境遭到严重破坏,导致野生资源的分布范围不断变窄,因此,有必要在未采集的适生地开展更大规模的收集工作[29-30]。本研究虽采集到广西8个市的河八王材料,但各地区材料主要集中在少数几个县,且缺乏广西另外6个地区(玉林市、崇左市、钦州市、南宁市、防城港市、北海市)的材料,因此,非常有必要加强对甘蔗种质资源适生区、富集区的系统调查,并对多样性丰富的区域进行补充性采集,加快完成我国优异野生甘蔗种质资源的收集和保护工作。
  • 国家重点研发计划项目(2021YFD1200204)
  • 国家自然科学基金项目(31901510)
  • 广西农业科学院科技发展基金项目(桂农科2022JM10)
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2025年第46卷第1期
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doi: 10.3969/j.issn.1000-2561.2025.01.010
  • 接收时间:2024-05-22
  • 首发时间:2026-06-24
  • 出版时间:2025-01-25
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  • 收稿日期:2024-05-22
  • 修回日期:2024-09-10
基金
国家重点研发计划项目(2021YFD1200204)
国家自然科学基金项目(31901510)
广西农业科学院科技发展基金项目(桂农科2022JM10)
作者信息
    1.广西壮族自治区农业科学院甘蔗研究所/广西甘蔗遗传改良重点实验室/农业农村部广西甘蔗生物技术与遗传改良重点实验室,广西南宁 530007
    2.广西大学农学院,广西南宁 530004

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* 杨细平(YANG Xiping),E-mail:
段维兴(DUAN Weixing),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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