Article(id=1276601064384237814, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1276600957765021779, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2024.06.001, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1686067200000, receivedDateStr=2023-06-07, revisedDate=1688054400000, revisedDateStr=2023-06-30, acceptedDate=null, acceptedDateStr=null, onlineDate=1782295011850, onlineDateStr=2026-06-24, pubDate=1719244800000, pubDateStr=2024-06-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1782295011850, onlineIssueDateStr=2026-06-24, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1782295011850, creator=13701087609, updateTime=1782295011850, updator=13701087609, issue=Issue{id=1276600957765021779, tenantId=1146029695717560320, journalId=1235980609244409860, year='2024', volume='45', issue='6', pageStart='1095', pageEnd='1302', issueExtLink='null', onlineDate='null', pubDate='1719244800000', pubDateStr='2024-06-25', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1782294986430, creator='13701087609', updateTime=1782348406834, updator='13701087609', preIssue=null, nextIssue=null, articleTotal=null, ext={EN=IssueExt(id=1276825019267285043, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1276600957765021779, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1276825019271479348, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1276600957765021779, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null, downloadFileDto=null}, startPage=1095, endPage=1101, ext={EN=ArticleExt(id=1276601064904331514, articleId=1276601064384237814, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Subcellular Localization and Multimerization Analyses of HbPIP1;4, a PIP Aquaporin from Rubber Tree (Hevea brasiliensis Muell. Arg.), columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Plasma membrane intrinsic proteins (PIPs), which belong to the aquaporin (AQP) family within the major intrinsic protein (MIP) superfamily, are known for the high water transport activity at the cell membrane. The subfamily is highly conservative and only includes two phylogenetic groups named PIP1 and PIP2. Natural rubber, an important industrial raw material and strategic material, is mainly derived from Para rubber tree (Hevea brasiliensis Muell. Arg.), a big tree originated in the Amazon basin of South America. As a special tissue for rubber synthesis and storage, the laticiferous cells possess no plasmodesmata with neighboring parenchyma cells, and the water balance is mainly mediated by PIP aquaporins. In previous studies, a highly abundant PIP gene denoted HbPIP1;4 was identified, however, no detectable water transport activity was found when heterologously expressed in Xenopus laevis oocytes, which may be due to its inability to localize to the cell membrane. To uncover the acting site and multimerization features of HbPIP1;4 in vivo, recombinant plasmids pNC-Cam1304-SubN-HbPIP1;4, pNC-BiFC-ECN-HbPIP1;4, and pNC-BiFC-ENN-HbPIP1;4 for subcellular localization and bimolecular fluorescence complementation (BiFC) were successfully constructed. Agrobacterium tumefaciens transformed with above recombinant plasmids were used to infiltrate tobacco (Nicotiana benthamiana), and transiently transformed leaves were subsequently checked using laser confocal microscopy. Compared with wide distribution of fluorescence signals throughout the cell for the control pNC-Cam1304-SubN, signals of pNC-Cam1304-GFP-HbPIP1;4 were restricted to the cell membrane, supporting the plasma membrane localization of HbPIP1;4, which is consistent with the bioinformatics prediction. The result was further supported by the BiFC analysis, which also implied that HbPIP1;4 could form homomultimer, in accordance with the 3D prediction via homology modeling. The results would lay a solid foundation for further uncovering the mechanism of water balance of the laticifer. Nevertheless, PIP aquaporins that could interact with HbPIP1;4 in rubber tree remain to be further characterized.

, authors=null, authorsList=Zhi ZOU, Yujiao ZHENG, Xueying QIAO, authorCompany=null, correspAuthors=null, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, fund=null), CN=ArticleExt(id=1276601069429985538, articleId=1276601064384237814, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=橡胶树水通道蛋白HbPIP1；4的亚细胞定位与多聚化分析, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

质膜内在蛋白（PIP）隶属于水通道蛋白家族，其以细胞膜定位并具有高效的水分转运活性而著称。PIP高度保守，主要包含PIP1和PIP2两个亚类。天然橡胶是重要的工业原料和战略物资，其主要来源为巴西橡胶树。作为橡胶合成和储藏的特异组织，乳管与邻近的薄壁细胞之间缺乏胞间连丝，其水分平衡主要由PIP介导。通过前期研究，团队鉴定到一个PIP1亚类成员HbPIP1；4，该基因在乳管中高水平表达，然而，其在蟾蜍卵母细胞中异源表达却无水分转运活性，推测因不能有效定位到细胞膜所致。为探讨HbPIP1；4在植物体内的功能部位及作用模式，本研究构建了其亚细胞定位和双分子荧光互补（BiFC）载体。通过利用农杆菌介导法瞬时转化烟草叶片，再用激光共聚焦显微镜进行观察，发现荧光信号出现在细胞膜，这与生物信息学预测的细胞膜定位结果一致。BiFC实验进一步证实HbPIP1；4定位在细胞膜，结果同时显示，HbPIP1；4可形成同源多聚体，这与3D结构预测结果一致。上述结果为深入揭示乳管的水分平衡机制奠定了坚实的基础。但HbPIP1；4的异源互作模式还有待进一步研究。

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邹智（1982—），男，研究员，研究方向：基因组进化与逆境适应机制、油脂代谢与调控；E-mail：zouzhi2008@126.com。

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邹智（1982—），男，研究员，研究方向：基因组进化与逆境适应机制、油脂代谢与调控；E-mail：zouzhi2008@126.com。

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The plasma membrane of Arabidopsis thaliana contains a mercury-insensitive aquaporin that is a homolog of the tonoplast water channel protein TIP[J]. Plant Physiology, 1994, 106(4): 1325-1333., articleTitle=The plasma membrane of Arabidopsis thaliana contains a mercury-insensitive aquaporin that is a homolog of the tonoplast water channel protein TIP, refAbstract=null), Reference(id=1276824408991859549, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2015, volume=16, issue=1, pageStart=1001, pageEnd=null, url=null, language=null, rfNumber=[2], rfOrder=1, authorNames=ZOU Z, GONG J, AN F, XIE G, WANG J, MO Y, YANG L, journalName=BMC Genomics, refType=null, unstructuredReference=ZOU Z, GONG J, AN F, XIE G, WANG J, MO Y, YANG L. Genome-wide identification of rubber tree (Hevea brasiliensis Muell. Arg.) aquaporin genes and their response to ethephon stimulation in the laticifer, a rubber-producing tissue[J]. BMC Genomics, 2015, 16(1): 1001., articleTitle=Genome-wide identification of rubber tree (Hevea brasiliensis Muell. Arg.) aquaporin genes and their response to ethephon stimulation in the laticifer, a rubber-producing tissue, refAbstract=null), Reference(id=1276824409058968414, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2015, volume=10, issue=10, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[3], rfOrder=2, authorNames=ZOU Z, GONG J, HUANG Q, MO Y Y, YANG L F, XIE G S, journalName=PLoS One, refType=null, unstructuredReference=ZOU Z, GONG J, HUANG Q, MO Y Y, YANG L F, XIE G S. Gene structures, evolution, classification and expression profiles of the aquaporin gene family in castor bean (Ricinus communis L.)[J]. PLoS One, 2015, 10(10): e0141022., articleTitle=Gene structures, evolution, classification and expression profiles of the aquaporin gene family in castor bean (Ricinus communis L.), refAbstract=null), Reference(id=1276824409251906399, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2016, volume=7, issue=null, pageStart=395, pageEnd=null, url=null, language=null, rfNumber=[4], rfOrder=3, authorNames=ZOU Z, YANG L F, GONG J, MO Y Y, WANG J K, CAO J H, AN F, XIE G S, journalName=Frontiers in Plant Science, refType=null, unstructuredReference=ZOU Z, YANG L F, GONG J, MO Y Y, WANG J K, CAO J H, AN F, XIE G S. Genome-wide identification of Jatropha curcas aquaporin genes and the comparative analysis provides insights into the gene family expansion and evolution in Hevea brasiliensis[J]. Frontiers in Plant Science, 2016, 7: 395., articleTitle=Genome-wide identification of Jatropha curcas aquaporin genes and the comparative analysis provides insights into the gene family expansion and evolution in Hevea brasiliensis, refAbstract=null), Reference(id=1276824409319015264, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2019, volume=20, issue=null, pageStart=380, pageEnd=null, url=null, language=null, rfNumber=[5], rfOrder=4, authorNames=ZOU Z, YANG J H, journalName=BMC Genomics, refType=null, unstructuredReference=ZOU Z, YANG J H. Genome-wide comparison reveals divergence of cassava and rubber aquaporin family genes after the recent whole-genome duplication[J]. BMC Genomics, 2019, 20: 380., articleTitle=Genome-wide comparison reveals divergence of cassava and rubber aquaporin family genes after the recent whole-genome duplication, refAbstract=null), Reference(id=1276824409390318433, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2006, volume=439, issue=7077, pageStart=688, pageEnd=694, url=null, language=null, rfNumber=[6], rfOrder=5, authorNames=TORNROTH-HORSEFIELD S, WANG Y, HEDFALK K, JOHANSON U, KARLSSON M, TAJKHORSHID E, NEUTZE R, KJELLBOM P, journalName=Nature, refType=null, unstructuredReference=TORNROTH-HORSEFIELD S, WANG Y, HEDFALK K, JOHANSON U, KARLSSON M, TAJKHORSHID E, NEUTZE R, KJELLBOM P. Structural mechanism of plant aquaporin gating[J]. Nature, 2006, 439(7077): 688-694., articleTitle=Structural mechanism of plant aquaporin gating, refAbstract=null), Reference(id=1276824409696502626, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2008, volume=415, issue=3, pageStart=409, pageEnd=416, url=null, language=null, rfNumber=[7], rfOrder=6, authorNames=VERDOUCQ L, GRONDIN A, MAUREL C, journalName=The Biochemical Journal, refType=null, unstructuredReference=VERDOUCQ L, GRONDIN A, MAUREL C. Structure-function analysis of plant aquaporin AtPIP2;1 gating by divalent cations and protons[J]. The Biochemical Journal, 2008, 415(3): 409-416., articleTitle=Structure-function analysis of plant aquaporin AtPIP2;1 gating by divalent cations and protons, refAbstract=null), Reference(id=1276824409759417187, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2020, volume=1862, issue=2, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[8], rfOrder=7, authorNames=WANG H, SCHOEBEL S, SCHMITZ F, DONG H, HEDFALK K, journalName=Biochimica et Biophysica Acta (BBA)–Biomembranes, refType=null, unstructuredReference=WANG H, SCHOEBEL S, SCHMITZ F, DONG H, HEDFALK K. Characterization of aquaporin-driven hydrogen peroxide transport[J]. Biochimica et Biophysica Acta (BBA)–Biomembranes, 2020, 1862(2): 183065., articleTitle=Characterization of aquaporin-driven hydrogen peroxide transport, refAbstract=null), Reference(id=1276824409830720356, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2004, volume=16, issue=1, pageStart=215, pageEnd=228, url=null, language=null, rfNumber=[9], rfOrder=8, authorNames=FETTER K, VAN WILDER V, MOSHELION M, CHAUMONT F, journalName=The Plant Cell, refType=null, unstructuredReference=FETTER K, VAN WILDER V, MOSHELION M, CHAUMONT F. Interactions between plasma membrane aquaporins modulate their water channel activity[J]. The Plant Cell, 2004, 16(1): 215-228., articleTitle=Interactions between plasma membrane aquaporins modulate their water channel activity, refAbstract=null), Reference(id=1276824410157876069, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2007, volume=104, issue=30, pageStart=12359, pageEnd=12364, url=null, language=null, rfNumber=[10], rfOrder=9, authorNames=ZELAZNY E, BORST J W, MUYLAERT M, BATOKO H, HEMMINGA M A, CHAUMONT F, journalName=Proceedings of the National Academy of Sciences, refType=null, unstructuredReference=ZELAZNY E, BORST J W, MUYLAERT M, BATOKO H, HEMMINGA M A, CHAUMONT F. FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization[J]. Proceedings of the National Academy of Sciences, 2007, 104(30): 12359-12364., articleTitle=FRET imaging in living maize cells reveals that plasma membrane aquaporins interact to regulate their subcellular localization, refAbstract=null), Reference(id=1276824410497614694, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2009, volume=57, issue=2, pageStart=346, pageEnd=355, url=null, language=null, rfNumber=[11], rfOrder=10, authorNames=ZELAZNY E, MIECIELICA U, BORST J W, HEMMINGA M A, CHAUMONT F, journalName=Plant Journal, refType=null, unstructuredReference=ZELAZNY E, MIECIELICA U, BORST J W, HEMMINGA M A, CHAUMONT F. An N-terminal diacidic motif is required for the trafficking of maize aquaporins ZmPIP2;4 and ZmPIP2;5 to the plasma membrane[J]. Plant Journal, 2009, 57(2): 346-355., articleTitle=An N-terminal diacidic motif is required for the trafficking of maize aquaporins ZmPIP2;4 and ZmPIP2;5 to the plasma membrane, refAbstract=null), Reference(id=1276824410585695079, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2018, volume=9, issue=null, pageStart=382, pageEnd=null, url=null, language=null, rfNumber=[12], rfOrder=11, authorNames=BIENERT M D, DIEHN T A, RICHET N, CHAUMONT F, BIENERT G P, journalName=Frontiers in Plant Science, refType=null, unstructuredReference=BIENERT M D, DIEHN T A, RICHET N, CHAUMONT F, BIENERT G P. Heterotetramerization of plant PIP1 and PIP2 aquaporins is an evolutionary ancient feature to guide PIP1 plasma membrane localization and function[J]. Frontiers in Plant Science, 2018, 9: 382., articleTitle=Heterotetramerization of plant PIP1 and PIP2 aquaporins is an evolutionary ancient feature to guide PIP1 plasma membrane localization and function, refAbstract=null), Reference(id=1276824410950599528, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2010, volume=30, issue=1, pageStart=85, pageEnd=92, url=null, language=null, rfNumber=[13], rfOrder=12, authorNames=邹智, 杨礼富, 王真辉, 袁坤, journalName=中国生物工程杂志, refType=null, unstructuredReference=邹智, 杨礼富, 王真辉, 袁坤. 巴西橡胶树转基因研究现状与展望[J]. 中国生物工程杂志, 2010, 30(1): 85-92., articleTitle=巴西橡胶树转基因研究现状与展望, refAbstract=null), Reference(id=1276824411021902697, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2010, volume=30, issue=1, pageStart=85, pageEnd=92, url=null, language=null, rfNumber=[13], rfOrder=13, authorNames=ZOU Z, YANG L F, WANG Z H, YUAN K, journalName=Chinese Biotechnology, refType=null, unstructuredReference=ZOU Z, YANG L F, WANG Z H, YUAN K. Advances and prospects on genetic modification of Hevea brasiliensis[J]. Chinese Biotechnology, 2010, 30(1): 85-92. (in Chinese), articleTitle=Advances and prospects on genetic modification of Hevea brasiliensis, refAbstract=null), Reference(id=1276824411357447018, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2009, volume=45, issue=12, pageStart=1231, pageEnd=1238, url=null, language=null, rfNumber=[14], rfOrder=14, authorNames=邹智, 杨礼富, 王真辉, 袁坤, journalName=植物生理学通讯, refType=null, unstructuredReference=邹智, 杨礼富, 王真辉, 袁坤. 橡胶树中橡胶的生物合成与调控[J]. 植物生理学通讯, 2009, 45(12): 1231-1238., articleTitle=橡胶树中橡胶的生物合成与调控, refAbstract=null), Reference(id=1276824413051945836, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2009, volume=45, issue=12, pageStart=1231, pageEnd=1238, url=null, language=null, rfNumber=[14], rfOrder=15, authorNames=ZOU Z, YANG L F, WANG Z H, YUAN K, journalName=Plant Physiology Journal, refType=null, unstructuredReference=ZOU Z, YANG L F, WANG Z H, YUAN K. Biosynthesis and regulation of natural rubber in Hevea[J]. Plant Physiology Journal, 2009, 45(12): 1231-1238. (in Chinese), articleTitle=Biosynthesis and regulation of natural rubber in Hevea, refAbstract=null), Reference(id=1276824413114860397, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2024, volume=45, issue=3, pageStart=443, pageEnd=449, url=null, language=null, rfNumber=[15], rfOrder=16, authorNames=邹智, 乔雪莹, 郑玉皎, 阳江华, journalName=热带作物学报, refType=null, unstructuredReference=邹智, 乔雪莹, 郑玉皎, 阳江华. 橡胶树HbPIP2;3的亚细胞定位与多聚化分析[J]. 热带作物学报, 2024, 45(3): 443-449., articleTitle=橡胶树HbPIP2;3的亚细胞定位与多聚化分析, refAbstract=null), Reference(id=1276824413186163566, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2024, volume=45, issue=3, pageStart=443, pageEnd=449, url=null, language=null, rfNumber=[15], rfOrder=17, authorNames=ZOU Z, QIAO X Y, ZHENG Y J, YANG J H, journalName=Chinese Journal of Tropical Crops, refType=null, unstructuredReference=ZOU Z, QIAO X Y, ZHENG Y J, YANG J H. Subcellular localization and multimerization analyses of HbPIP2;3, an efficient water transporter from Hevea brasiliensis[J]. Chinese Journal of Tropical Crops, 2024, 45(3): 443-449. (in Chinese), articleTitle=Subcellular localization and multimerization analyses of HbPIP2;3, an efficient water transporter from Hevea brasiliensis, refAbstract=null), Reference(id=1276824413504930671, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2022, volume=43, issue=12, pageStart=2405, pageEnd=2412, url=null, language=null, rfNumber=[16], rfOrder=18, authorNames=乔雪莹, 郑玉皎, 阳江华, 曾长英, 邹智, journalName=热带作物学报, refType=null, unstructuredReference=乔雪莹, 郑玉皎, 阳江华, 曾长英, 邹智. 橡胶树HbPIP1;1基因的克隆及蛋白的亚细胞定位与多聚化分析[J]. 热带作物学报, 2022, 43(12): 2405-2412., articleTitle=橡胶树HbPIP1;1基因的克隆及蛋白的亚细胞定位与多聚化分析, refAbstract=null), Reference(id=1276824413593011056, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2022, volume=43, issue=12, pageStart=2405, pageEnd=2412, url=null, language=null, rfNumber=[16], rfOrder=19, authorNames=QIAO X Y, ZHENG Y J, YANG J H, ZENG C Y, ZOU Z, journalName=Chinese Journal of Tropical Crops, refType=null, unstructuredReference=QIAO X Y, ZHENG Y J, YANG J H, ZENG C Y, ZOU Z. Gene cloning, subcellular localization and multimerization analysis of HbPIP1;1 from Hevea brasiliensis[J]. Chinese Journal of Tropical Crops, 2022, 43(12): 2405-2412. (in Chinese), articleTitle=Gene cloning, subcellular localization and multimerization analysis of HbPIP1;1 from Hevea brasiliensis, refAbstract=null), Reference(id=1276824413874029425, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2015, volume=10, issue=4, pageStart=null, pageEnd=null, url=null, language=null, rfNumber=[17], rfOrder=20, authorNames=AN F, ZOU Z, CAI X Q, WANG J, ROOKES J, LIN W F, CAHILL D, KONG L X, journalName=PLoS One, refType=null, unstructuredReference=AN F, ZOU Z, CAI X Q, WANG J, ROOKES J, LIN W F, CAHILL D, KONG L X. Regulation of HbPIP2;3, a latex-abundant water transporter, is associated with latex dilution and yield in the rubber tree (Hevea brasiliensis Muell. Arg.)[J]. PLoS One, 2015, 10(4): e0125595., articleTitle=Regulation of HbPIP2;3, a latex-abundant water transporter, is associated with latex dilution and yield in the rubber tree (Hevea brasiliensis Muell. Arg.), refAbstract=null), Reference(id=1276824413936943986, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2010, volume=31, issue=10, pageStart=1752, pageEnd=1756, url=null, language=null, rfNumber=[18], rfOrder=21, authorNames=邹智, 杨礼富, journalName=热带作物学报, refType=null, unstructuredReference=邹智, 杨礼富. 巴西橡胶树铁硫簇支架蛋白cDNA的克隆与分析[J]. 热带作物学报, 2010, 31(10): 1752-1756., articleTitle=巴西橡胶树铁硫簇支架蛋白cDNA的克隆与分析, refAbstract=null), Reference(id=1276824413995664243, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2010, volume=31, issue=10, pageStart=1752, pageEnd=1756, url=null, language=null, rfNumber=[18], rfOrder=22, authorNames=ZOU Z, YANG L F, journalName=Chinese Journal of Tropical Crops, refType=null, unstructuredReference=ZOU Z, YANG L F. Cloning and analysis of a cDNA encoding an iron-sulfur cluster scaffold protein ISU1 from Hevea brasiliensis Muell. Arg.[J]. Chinese Journal of Tropical Crops, 2010, 31(10): 1752-1756. (in Chinese), articleTitle=Cloning and analysis of a cDNA encoding an iron-sulfur cluster scaffold protein ISU1 from Hevea brasiliensis Muell. Arg., refAbstract=null), Reference(id=1276824414050190196, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2022, volume=43, issue=5, pageStart=923, pageEnd=929, url=null, language=null, rfNumber=[19], rfOrder=23, authorNames=徐硕, 邹智, 肖艳华, 张丽, 孔华, 郭静远, 郭安平, journalName=热带作物学报, refType=null, unstructuredReference=徐硕, 邹智, 肖艳华, 张丽, 孔华, 郭静远, 郭安平. 油莎豆块茎油脂积累相关基因CeWRI1的克隆与功能分析[J]. 热带作物学报, 2022, 43(5): 923-929., articleTitle=油莎豆块茎油脂积累相关基因CeWRI1的克隆与功能分析, refAbstract=null), Reference(id=1276824414289265525, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2022, volume=43, issue=5, pageStart=923, pageEnd=929, url=null, language=null, rfNumber=[19], rfOrder=24, authorNames=XU S, ZOU Z, XIAO Y H, ZHANG L, KONG H, GUO J Y, GUO A P, journalName=Chinese Journal of Tropical Crops, refType=null, unstructuredReference=XU S, ZOU Z, XIAO Y H, ZHANG L, KONG H, GUO J Y, GUO A P. Cloning and functional characterization of CeWRI1, a gene involved in oil accumulation from tigernut (Cyperus esculentus L.) tubers[J]. Chinese Journal of Tropical Crops, 2022, 43(5): 923-929. (in Chinese), articleTitle=Cloning and functional characterization of CeWRI1, a gene involved in oil accumulation from tigernut (Cyperus esculentus L.) tubers, refAbstract=null), Reference(id=1276824414352180086, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2023, volume=42, issue=11, pageStart=1791, pageEnd=1808, url=null, language=null, rfNumber=[20], rfOrder=25, authorNames=ZOU Z, ZHENG Y J, ZHANG Z T, XIAO Y H, XIE Z N, CHANG L L, ZHANG L, ZHAO Y G, journalName=Plant Cell Reports, refType=null, unstructuredReference=ZOU Z, ZHENG Y J, ZHANG Z T, XIAO Y H, XIE Z N, CHANG L L, ZHANG L, ZHAO Y G. Molecular characterization of oleosin genes in Cyperus esculentus, a Cyperaceae plant producing oil in underground tubers[J]. Plant Cell Reports, 2023, 42(11): 1791-1808., articleTitle=Molecular characterization of oleosin genes in Cyperus esculentus, a Cyperaceae plant producing oil in underground tubers, refAbstract=null), Reference(id=1276824414419288951, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2011, volume=168, issue=3, pageStart=253, pageEnd=262, url=null, language=null, rfNumber=[21], rfOrder=26, authorNames=TUNGNGOEN K, VIBOONJUN U, KONGSAWADWO RAKUL P, KATSUHARA M, JULIEN J L, SAKR S, CHRESTIN H, NARANGAJAVANA J, journalName=Journal of Plant Physiology, refType=null, unstructuredReference=TUNGNGOEN K, VIBOONJUN U, KONGSAWADWO RAKUL P, KATSUHARA M, JULIEN J L, SAKR S, CHRESTIN H, NARANGAJAVANA J. Hormonal treatment of the bark of rubber trees (Hevea brasiliensis) increases latex yield through latex dilution in relation with the differential expression of two aquaporin genes[J]. Journal of Plant Physiology, 2011, 168(3): 253-262., articleTitle=Hormonal treatment of the bark of rubber trees (Hevea brasiliensis) increases latex yield through latex dilution in relation with the differential expression of two aquaporin genes, refAbstract=null), Reference(id=1276824414712890232, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2024, volume=44, issue=1, pageStart=1, pageEnd=6, url=null, language=null, rfNumber=[22], rfOrder=27, authorNames=邹智, 郑玉皎, 乔雪莹, 阳江华, journalName=西南林业大学学报(自然科学), refType=null, unstructuredReference=邹智, 郑玉皎, 乔雪莹, 阳江华. 橡胶树水通道蛋白HbPIP2;7的亚细胞定位与多聚化分析[J]. 西南林业大学学报(自然科学), 2024, 44(1): 1-6., articleTitle=橡胶树水通道蛋白HbPIP2;7的亚细胞定位与多聚化分析, refAbstract=null), Reference(id=1276824414788387705, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, doi=null, pmid=null, pmcid=null, year=2024, volume=44, issue=1, pageStart=1, pageEnd=6, url=null, language=null, rfNumber=[22], rfOrder=28, authorNames=ZOU Z, ZHENG Y J, QIAO X Y, YANG J H, journalName=Journal of Southwest Forestry University (Natural Science), refType=null, unstructuredReference=ZOU Z, ZHENG Y J, QIAO X Y, YANG J H. Subcellular localization and multimerization analyses of HbPIP2;7, a PIP aquaporin from rubber tree (Hevea brasiliensis Muell. Arg.)[J]. Journal of Southwest Forestry University (Natural Science), 2024, 44(1): 1-6. (in Chinese), articleTitle=Subcellular localization and multimerization analyses of HbPIP2;7, a PIP aquaporin from rubber tree (Hevea brasiliensis Muell. 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A: Predicted conservative domain for HbPIP1;4; B: Comparison of amino acid composition of HbPIP1;4, HbPIP1;1, HbPIP2;3, SoPIP2;1, AtPIP2;1 and AtPIP2;4; C: Sequence alignment of HbPIP1;4, HbPIP1;1, HbPIP2;3, SoPIP2;1, AtPIP2;1 and AtPIP2;4; D: Predicted 3D structure for HbPIP1;4.

, figureFileSmall=fQdH1fyKfpEJNNtsX3I8yA==, figureFileBig=H2BWBFeFfUXovR0MJ9IiOg==, tableContent=null), ArticleFig(id=1276824404323599183, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, language=CN, label=图1, caption=HbPIP1；4的生物信息学分析

A：HbPIP1;4的保守结构域预测；B：HbPIP1;4、HbPIP1;1、HbPIP2;3、SoPIP2;1、AtPIP2;1和AtPIP2;4的氨基酸组成比较；C：HbPIP1;4、HbPIP1;1、HbPIP2;3、SoPIP2;1、AtPIP2;1和AtPIP2;4的序列比对；D：HbPIP1;4的3D结构预测。

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Databases, software and related websites used in this study

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数据库/软件Database/software	网址Website	用途Application
NCBI	https://www.ncbi.nlm.nih.gov/	PIP蛋白下载
BLAST	https://blast.ncbi.nlm.nih.gov/Blast.cgi	同源分析
MUSCLE	https://mega6.software.informer.com/	蛋白多序列比对
ProtParam	https://web.expasy.org/protparam/	蛋白理化特性分析
WoLF PSORT	https://www.genscript.com/wolf-psort.html	亚细胞定位预测
CDD	https://www.ncbi.nlm.nih.gov/cdd	保守结构域预测
SOPMA	https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSA/npsa_sopma.html	二级结构预测
SWISS-MODEL	https://swissmodel.expasy.org/interactive	3D结构预测
InterPro	http://www.ebi.ac.uk/interpro/search/	生物学功能预测

), ArticleFig(id=1276824406018098005, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, language=CN, label=表1, caption=

本研究所用数据库、软件及其网址

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数据库/软件Database/software	网址Website	用途Application
NCBI	https://www.ncbi.nlm.nih.gov/	PIP蛋白下载
BLAST	https://blast.ncbi.nlm.nih.gov/Blast.cgi	同源分析
MUSCLE	https://mega6.software.informer.com/	蛋白多序列比对
ProtParam	https://web.expasy.org/protparam/	蛋白理化特性分析
WoLF PSORT	https://www.genscript.com/wolf-psort.html	亚细胞定位预测
CDD	https://www.ncbi.nlm.nih.gov/cdd	保守结构域预测
SOPMA	https://npsa-prabi.ibcp.fr/cgi-bin/npsa_automat.pl?page=/NPSA/npsa_sopma.html	二级结构预测
SWISS-MODEL	https://swissmodel.expasy.org/interactive	3D结构预测
InterPro	http://www.ebi.ac.uk/interpro/search/	生物学功能预测

), ArticleFig(id=1276824406320087894, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, language=EN, label=Tab. 2, caption=

Comparison of physicochemical properties and secondary structure of HbPIP1;4, HbPIP1;1, HbPIP2;3, SoPIP2;1, AtPIP2;1 and AtPIP2;4

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蛋白Protein	aa	MW/kDa	pI	GRAVY	AI	II	MIP	α-螺旋Alpha helix/%	延伸链Extended strand/%	β-转角Beta turn/%	无规则卷曲Random coil/%
HbPIP1;4	287	30.80	8.59	0.334	95.89	28.42	44~274	32.40	17.42	2.44	47.74
HbPIP1;1	287	30.78	8.62	0.352	93.90	27.46	46~276	30.66	18.47	3.14	47.74
HbPIP2;3	286	30.58	8.50	0.449	100.38	31.68	32~267	36.36	17.48	3.50	42.66
SoPIP2;1	281	29.90	9.03	0.579	102.81	27.81	31~260	36.65	18.15	2.14	43.06
AtPIP2;1	287	30.47	8.60	0.506	97.94	30.27	30~266	40.42	16.72	4.88	37.98
AtPIP2;4	291	30.95	8.22	0.490	96.29	29.29	30~266	30.24	20.96	5.15	43.64

), ArticleFig(id=1276824406424945495, tenantId=1146029695717560320, journalId=1235980609244409860, articleId=1276601064384237814, language=CN, label=表2, caption=

HbPIP1；4与HbPIP1；1、HbPIP2；3、SoPIP2；1、AtPIP2；1和AtPIP2；4理化特性和二级结构比较

, figureFileSmall=null, figureFileBig=null, tableContent=

蛋白Protein	aa	MW/kDa	pI	GRAVY	AI	II	MIP	α-螺旋Alpha helix/%	延伸链Extended strand/%	β-转角Beta turn/%	无规则卷曲Random coil/%
HbPIP1;4	287	30.80	8.59	0.334	95.89	28.42	44~274	32.40	17.42	2.44	47.74
HbPIP1;1	287	30.78	8.62	0.352	93.90	27.46	46~276	30.66	18.47	3.14	47.74
HbPIP2;3	286	30.58	8.50	0.449	100.38	31.68	32~267	36.36	17.48	3.50	42.66
SoPIP2;1	281	29.90	9.03	0.579	102.81	27.81	31~260	36.65	18.15	2.14	43.06
AtPIP2;1	287	30.47	8.60	0.506	97.94	30.27	30~266	40.42	16.72	4.88	37.98
AtPIP2;4	291	30.95	8.22	0.490	96.29	29.29	30~266	30.24	20.96	5.15	43.64

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