Article(id=1236367340812686252, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236367331312595268, articleNumber=null, orderNo=null, doi=10.3969/j.issn.1000-2561.2023.04.004, pmid=null, cstr=null, oa=null, hot=null, price=null, onlineType=0, articleFormat=0, articleType=null, articleTypeStr=null, receivedDate=1655740800000, receivedDateStr=2022-06-21, revisedDate=1657468800000, revisedDateStr=2022-07-11, acceptedDate=null, acceptedDateStr=null, onlineDate=1772702544644, onlineDateStr=2026-03-05, pubDate=1682352000000, pubDateStr=2023-04-25, doiRegisterDate=null, doiRegisterDateStr=null, onlineIssueDate=1772702544644, onlineIssueDateStr=2026-03-05, onlineJustAcceptDate=null, onlineJustAcceptDateStr=null, onlineFirstDate=null, onlineFirstDateStr=null, sourceXml=null, magXml=null, createTime=1772702544644, creator=13701087609, updateTime=1772702544644, updator=13701087609, issue=Issue{id=1236367331312595268, tenantId=1146029695717560320, journalId=1235980609244409860, year='2023', volume='44', issue='4', pageStart='661', pageEnd='866', issueExtLink='null', onlineDate='null', pubDate='null', beforeIssueId=null, nextIssueId=null, price=null, status=1, issueComplete=1, articleOrder=1, issueType=-1, specialIssue=null, createTime=1772702542379, creator=13701087609, updateTime=1772702636855, updator=13701087609, preIssue=null, nextIssue=null, ext={EN=IssueExt(id=1236367727644955170, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236367331312595268, language=EN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=), CN=IssueExt(id=1236367727644955171, tenantId=1146029695717560320, journalId=1235980609244409860, issueId=1236367331312595268, language=CN, specialIssueTitle=, coverIllustrator=null, specialIssueEditor=, specialIssueAbout=)}, issueFiles=null}, startPage=689, endPage=698, ext={EN=ArticleExt(id=1236367341076927426, articleId=1236367340812686252, tenantId=1146029695717560320, journalId=1235980609244409860, language=EN, title=Characterization and Phylogenetic Analysis of the Complete Chloplast Genome of Erigeron annuus, columnId=1236256430337085821, journalTitle=Chinese Journal of Tropical Crops, columnName=Omics & Biotechnology, runingTitle=null, highlight=null, articleAbstract=

Erigeron annuus, a member of the Asteraceae family, is widely distributed in tropic, subtropical and temperate regions of China. It is a plant native to North America, which was introduced to China at the end of the nineteenth century as an ornamental plant. E. annuus has great medicinal value. Its whole plant has been used in folk medicine for the treatment of indigestion, enteritis, epidemic hepatitis, lymphadenitis, hematuria, acute inflammation, malaria and obesity. E. annuus could be a phytoremediator for metal- and metalloid-contaminated soils. It can degrade diesel effectively, has strong tolerance and accumulation for uranium, cadmium, zinc, lead, and antimony, and can be distributed in soils with high pollution levels. Hence, In this study, it was to explore the characteristics of chloroplast (cp) genome and its phylogenetic relationship, laying a foundation for the further development of species identification, control and full utilization of E. annuus. The complete cp genome of E. annuus was assembled and sequenced by Illumina Nova Seq 6000 Sequencing platform and SPAdes v3.10. software. Taking the cp genome of E. canadensis (MT806101.1) as a reference, the genome feature and phylogenetic analysis study were investigated. The cp genome of E. annuus was a circular and quadripartite structure with a total length of 153 283 bp. The GC and AT content of the cp genome was 36.77%and 63.23%, respectively. The cp genome of E. annuus had four typical regions, containing a pair of inverted repeats (IRs, 25 019 bp) that were separated by the large single-copy (LSC, 84 833 bp) and small single-copy (SSC, 18 412 bp). In total, 132 genes were predicted, consisting of 88 protein-coding genes, 36 transfer RNA genes, and 8 ribosomal RNA genes. The genes were divided into 4 categories according to the functions, including 45 photosynthesis genes, 73 self-replication genes, 6 other genes and 8 genes of unknown function. SSR site analysis revealed that the cp genome sequence contained 200 SSR loci in total, which were spaced disproportionately through the genome with the largest number of SSRs distributed in the LSC region, followed by the SSC and IR regions. LSC, SSC and IR regions had 126, 40, and 34 SSRs, respectively. The number of SSRs in LSC, SSC and IR regions was accounted with 63%, 20% and 17%, respectively. The SSRs were mononucleotides, especially for A/T. The second most abundant motif type was the trinucleotide type, especially TAA, TAT and TTA. Phylogenetic analysis showed that E. annuus was closely related to the plant of E. canadensis. This study revealed the cp genomic information of E. annuus and would provide essential data for further studies on genetics, phylogeny, utilization and prevention of this species. This study revealed the chloroplast genome information and identified the phylogenetic branches of E. annuus, which would provide molecular theoretical support for further research on population genetics, phylogenetic geography, adaptive mechanism and management of E. annuus.

, correspAuthors=Yihui GONG, authorNote=null, correspAuthorsNote=null, copyrightStatement=null, copyrightOwner=null, extLink=null, articleAbsUrl=null, sourceXml=null, magXml=null, pdfUrl=null, pdf=null, pdfFileSize=null, pdfExtLink=null, richHtmlUrl=null, mobilePdfUrl=null, reviewReport=null, pdfFirstPage=null, abstractGraph=null, abstractGraphContent=null, abstractVideo=null, citation=null, cebUrl=null, magXmlContent=null, mapNumber=null, authorCompany=null, fund=null, authors=null, authorsList=Yunyun ZHOU, Min GUO, Yingquan PENG, Chenzhong JIN, Xuejiao ZHANG, Yihong HU, Gong CHEN, Yihui GONG), CN=ArticleExt(id=1236367341408277465, articleId=1236367340812686252, tenantId=1146029695717560320, journalId=1235980609244409860, language=CN, title=一年蓬叶绿体基因组特征及系统进化分析, columnId=1236256430517440904, journalTitle=热带作物学报, columnName=组学与生物技术, runingTitle=null, highlight=null, articleAbstract=

一年蓬(Erigeron annuus)作为一种入侵植物,对我国农林业发展、本地物种多样性及生态系统具有严重的影响。本研究利用Illumina NovaSeq 6000测定一年蓬的DNA序列,采用SPAdes v3.10.1软件组装一年蓬叶绿体基因组,以小蓬草(Erigeron canadensis, MT806101.1)叶绿体基因组为参考,对一年蓬叶绿体基因组结构、特征、SSR位点、IR边界以及进化树进行分析,结果表明:一年蓬叶绿体基因组全长为153 283 bp,AT、GC含量分别为62.95%、37.05%。包括大单拷贝区(LSC)84 833 bp,小单拷贝区(SSC)18 412 bp,反向重复区(IRa和IRb)25 019 bp,一年蓬叶绿体基因组共编码到132个基因,包括蛋白编码基因88个,转运RNA基因36个,核糖体RNA基因8个。按照功能分类将它们分成四大类:光合作用相关基因共45个、自我复制相关基因共73个、其他基因共6个、未知功能基因共8个。在一年蓬叶绿体基因组中共查找200个符合条件的SSR位点,分布在LSC、SSC、IR区域的SSR数量分别为126、40、34个,分别占总SSR位点数比重为63%、20%、17%,以单核苷酸重复占绝对优势,主要是A/T碱基,其次是三核苷酸重复单元类型,主要是TTA、TAA和TAT。进化树分析表明,一年蓬与小篷草亲缘关系最近。本研究揭示了一年蓬叶绿体基因组信息,并确定了一年蓬系统发育所属分支,为进一步开展一年蓬种群遗传学、系统发育地理学、适应性机制及防控与利用等研究提供分子理论支撑。

, correspAuthors=龚意辉, authorNote=null, correspAuthorsNote=
* 龚意辉(GONGYihui),E-mail:
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周芸芸(1986—),女,博士,讲师,研究方向:野生动植物资源管理和分子生态学。

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周芸芸(1986—),女,博士,讲师,研究方向:野生动植物资源管理和分子生态学。

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周芸芸(1986—),女,博士,讲师,研究方向:野生动植物资源管理和分子生态学。

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一年蓬叶绿体基因组特征及系统进化分析
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周芸芸 1 , 郭敏 1 , 彭英权 1 , 金晨钟 1 , 张雪娇 2 , 胡一鸿 1 , 陈功 1 , 龚意辉 1, *
热带作物学报 | 组学与生物技术 2023,44(4): 689-698
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热带作物学报 | 组学与生物技术 2023, 44(4): 689-698
一年蓬叶绿体基因组特征及系统进化分析
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周芸芸1, 郭敏1, 彭英权1, 金晨钟1, 张雪娇2, 胡一鸿1, 陈功1, 龚意辉1, *
作者信息
  • 1.湖南省农田杂草防控技术与应用协同创新中心/湖南人文科技学院农作物有害生物绿色防控重点实验室,湖南娄底 417000
  • 2.邵阳学院,湖南邵阳 422000
  • 周芸芸(1986—),女,博士,讲师,研究方向:野生动植物资源管理和分子生态学。

通讯作者:

* 龚意辉(GONGYihui),E-mail:
Characterization and Phylogenetic Analysis of the Complete Chloplast Genome of Erigeron annuus
Yunyun ZHOU1, Min GUO1, Yingquan PENG1, Chenzhong JIN1, Xuejiao ZHANG2, Yihong HU1, Gong CHEN1, Yihui GONG1, *
Affiliations
  • 1.Hunan Provincial Collaborative Innovation Center for Field Weeds Control/Key Laboratory of Green Control of Crop Pests in Hunan Higher Education, Hunan University of Humanities, Science and Technology, Loudi, Hunan 417000, China
  • 2.Shaoyang University, Shaoyang, Hunan 422000, China
出版时间: 2023-04-25 doi: 10.3969/j.issn.1000-2561.2023.04.004
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一年蓬(Erigeron annuus)作为一种入侵植物,对我国农林业发展、本地物种多样性及生态系统具有严重的影响。本研究利用Illumina NovaSeq 6000测定一年蓬的DNA序列,采用SPAdes v3.10.1软件组装一年蓬叶绿体基因组,以小蓬草(Erigeron canadensis, MT806101.1)叶绿体基因组为参考,对一年蓬叶绿体基因组结构、特征、SSR位点、IR边界以及进化树进行分析,结果表明:一年蓬叶绿体基因组全长为153 283 bp,AT、GC含量分别为62.95%、37.05%。包括大单拷贝区(LSC)84 833 bp,小单拷贝区(SSC)18 412 bp,反向重复区(IRa和IRb)25 019 bp,一年蓬叶绿体基因组共编码到132个基因,包括蛋白编码基因88个,转运RNA基因36个,核糖体RNA基因8个。按照功能分类将它们分成四大类:光合作用相关基因共45个、自我复制相关基因共73个、其他基因共6个、未知功能基因共8个。在一年蓬叶绿体基因组中共查找200个符合条件的SSR位点,分布在LSC、SSC、IR区域的SSR数量分别为126、40、34个,分别占总SSR位点数比重为63%、20%、17%,以单核苷酸重复占绝对优势,主要是A/T碱基,其次是三核苷酸重复单元类型,主要是TTA、TAA和TAT。进化树分析表明,一年蓬与小篷草亲缘关系最近。本研究揭示了一年蓬叶绿体基因组信息,并确定了一年蓬系统发育所属分支,为进一步开展一年蓬种群遗传学、系统发育地理学、适应性机制及防控与利用等研究提供分子理论支撑。

一年蓬  /  叶绿体  /  基因组  /  结构特征  /  系统发育

Erigeron annuus, a member of the Asteraceae family, is widely distributed in tropic, subtropical and temperate regions of China. It is a plant native to North America, which was introduced to China at the end of the nineteenth century as an ornamental plant. E. annuus has great medicinal value. Its whole plant has been used in folk medicine for the treatment of indigestion, enteritis, epidemic hepatitis, lymphadenitis, hematuria, acute inflammation, malaria and obesity. E. annuus could be a phytoremediator for metal- and metalloid-contaminated soils. It can degrade diesel effectively, has strong tolerance and accumulation for uranium, cadmium, zinc, lead, and antimony, and can be distributed in soils with high pollution levels. Hence, In this study, it was to explore the characteristics of chloroplast (cp) genome and its phylogenetic relationship, laying a foundation for the further development of species identification, control and full utilization of E. annuus. The complete cp genome of E. annuus was assembled and sequenced by Illumina Nova Seq 6000 Sequencing platform and SPAdes v3.10. software. Taking the cp genome of E. canadensis (MT806101.1) as a reference, the genome feature and phylogenetic analysis study were investigated. The cp genome of E. annuus was a circular and quadripartite structure with a total length of 153 283 bp. The GC and AT content of the cp genome was 36.77%and 63.23%, respectively. The cp genome of E. annuus had four typical regions, containing a pair of inverted repeats (IRs, 25 019 bp) that were separated by the large single-copy (LSC, 84 833 bp) and small single-copy (SSC, 18 412 bp). In total, 132 genes were predicted, consisting of 88 protein-coding genes, 36 transfer RNA genes, and 8 ribosomal RNA genes. The genes were divided into 4 categories according to the functions, including 45 photosynthesis genes, 73 self-replication genes, 6 other genes and 8 genes of unknown function. SSR site analysis revealed that the cp genome sequence contained 200 SSR loci in total, which were spaced disproportionately through the genome with the largest number of SSRs distributed in the LSC region, followed by the SSC and IR regions. LSC, SSC and IR regions had 126, 40, and 34 SSRs, respectively. The number of SSRs in LSC, SSC and IR regions was accounted with 63%, 20% and 17%, respectively. The SSRs were mononucleotides, especially for A/T. The second most abundant motif type was the trinucleotide type, especially TAA, TAT and TTA. Phylogenetic analysis showed that E. annuus was closely related to the plant of E. canadensis. This study revealed the cp genomic information of E. annuus and would provide essential data for further studies on genetics, phylogeny, utilization and prevention of this species. This study revealed the chloroplast genome information and identified the phylogenetic branches of E. annuus, which would provide molecular theoretical support for further research on population genetics, phylogenetic geography, adaptive mechanism and management of E. annuus.

Erigeron annuus  /  chloroplast  /  genome  /  structure characteristics  /  phylogenetic
周芸芸, 郭敏, 彭英权, 金晨钟, 张雪娇, 胡一鸿, 陈功, 龚意辉. 一年蓬叶绿体基因组特征及系统进化分析. 热带作物学报, 2023 , 44 (4) : 689 -698 . DOI: 10.3969/j.issn.1000-2561.2023.04.004
Yunyun ZHOU, Min GUO, Yingquan PENG, Chenzhong JIN, Xuejiao ZHANG, Yihong HU, Gong CHEN, Yihui GONG. Characterization and Phylogenetic Analysis of the Complete Chloplast Genome of Erigeron annuus[J]. Chinese Journal of Tropical Crops, 2023 , 44 (4) : 689 -698 . DOI: 10.3969/j.issn.1000-2561.2023.04.004
  • 湖南省科技创新计划项目(2019NK4170)
  • 湖南省教育厅科学研究重点项目(20A281)
  • 湖南省教育厅重点项目(21A0480)
2023年第44卷第4期
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doi: 10.3969/j.issn.1000-2561.2023.04.004
  • 接收时间:2022-06-21
  • 首发时间:2026-03-05
  • 出版时间:2023-04-25
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  • 收稿日期:2022-06-21
  • 修回日期:2022-07-11
基金
湖南省科技创新计划项目(2019NK4170)
湖南省教育厅科学研究重点项目(20A281)
湖南省教育厅重点项目(21A0480)
作者信息
    1.湖南省农田杂草防控技术与应用协同创新中心/湖南人文科技学院农作物有害生物绿色防控重点实验室,湖南娄底 417000
    2.邵阳学院,湖南邵阳 422000

通讯作者:

* 龚意辉(GONGYihui),E-mail:
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2种不同金属材料的力学参数

Family
属数
Number of
genus
种数
Number of
species
占总种数比例
Percentage of
total species (%)

Genus
种数
Number of
species
占总种数比例
Percentage of total
species (%)
鹅膏菌科Amanitaceae 2 11 5.26 鹅膏菌属 Amanita 10 4.78
小菇科 Mycenaceae 2 12 5.74 丝盖伞属 Inocybe 5 2.39
多孔菌科 Polyporaceae 8 14 6.70 蜡蘑属 Laccaria 5 2.39
红菇科 Russulaceae 3 23 11.00 小皮伞属 Marasmius 6 2.87
小菇属 Mycena 11 5.26
光柄菇属 Pluteus 5 2.39
红菇属 Russula 17 8.13
栓菌属 Trametes 5 2.39
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